Objective To improve the current method of spermatogonial cells transplantation, and make the new method become more operative and easy to carry out. Methods The spermatogonial cells in rC57BL6/tg14 (act-EGFO-Osb Y01) mice that expresse GFP protein were collected as the donor cells, and by using a modified syringe, and then were injected into the seminiferous tubules of pretreated wild type GFP-null mice through testicular efferent duct. The transplantation outcome was evaluated by trypan blue stainting and fluorescent microscopic examination and pathohistological analysis of transplanted testis. Results The transplanted testis of recipient mice showed green fluorescence signal, and the signal of seminiferous tubules was found significantly higher than that of the surrounding tissues. The transplanted GFP-positive cells generated colonies and spermatogenesis but pretreated wild type GFP-null mice were not found. Conclusion The expressed GFP protein spermatogonial cells in rC57BL6/tg14 mice were successfully transplanted in the wild type GFP-null recipient mice, fourthermore the improved transplantation method simplified the triditional one and achieved the same transplantation results.

Objective:To develop a domain-specific perfectionism scale for college students.Methods:Exploratory and confirmatory factor analytic approaches were used to develop and evaluate the intra-structure of the scale.Results:Exploratory principle factor analysis of the items indicated that the scale had five factors,explaining 57.538% of the total variance.Internal consistency analysis showed that the Cronbach's ? coefficient,split-half and test-retest correlation coefficients were 0.648～0.781,0.664～0.790,0.720～0.779 respectively for the factors.Confirmatory fator analysis showed that the fit indexes for ?2/df,GFI,AGFI,NFI,NNFI,RMSEA,CFI,IFI were 2.32,0.91,0.88,0.84,0.88,0.06,0.90,0.90.Conclusion:Psychometric properties analysis shows that the scale achieves from good to exellent levels of internal consistency,test-retest rliability and content,construct and criteria-related validity.

Antiviral Agents ; administration & dosage ; therapeutic use ; Child ; Drug Therapy, Combination ; Hepatitis B, Chronic ; drug therapy ; Hepatitis C, Chronic ; drug therapy ; Humans ; Interferon-alpha ; administration & dosage ; therapeutic use ; Lamivudine ; administration & dosage ; therapeutic use ; Nucleosides ; administration & dosage ; therapeutic use ; Polyethylene Glycols ; administration & dosage ; therapeutic use

The antiviral treatment for children with chronic hepatitis B virus(HBV) infection should be started in the immune active phase.Interferon (IFN) is the drug of first choice in most cases,however,the individual treatment and management of adverse effects need to be considered.Currently,Nucleos (t)ide analogues (NAs) can be used as the first choice only for those special children who can't use IFN.Efficacy will be seriously affected once the emergence of viral resistance mutants to NAs.However,there is high risk of relapse after the NAs are discontinued.Compared with the mono therapy,the therapeutic strategy of interferon combined with NAs have obtained better effect and safety in children with chronic HBV infection.

By electron microscopic observation,we found few pit cells in liver,spleen.lungs,and small intestine in Wistar rat.The pit cells show several morphologicalcharacteristics as follows:1.electron dense granules in cytoplasm,which aresurrounded by a limiting membrane;2.vesicles with rod shaped bodies incytoplasm;3.eccentric nucleus with marginal heterochromatin;4.well developedpseudopodia.In addition,the pit cells have also been found in breast carcinoma in3 patients.According to their distribution and morphology,we preliminarilydeduced they probably belong to natural killer cells and may act as a cytotoxicagent and an immunological defender against carcinoma.

Objective To evaluate parathyroidectomy combined with parathyroid tissue transplantation in forearm in the treatment of severe secondary hyperparathyroidism (SHP). Methods 9 cases treated with the procedure were reviewed.The diagnosis,operation indication and peri operative management were discussed. Results Thirty five hypertrophic parathyroids were resected from the 9 cases with SHP,and a left thyroidectomy was carried out for one case.Some parathyroid tissue has been grafted into the forearm for every patient.All of those grafts have survived.The symptoms va nished in all and the relevent laboratory tests recovered to normal after the surgery. Conclusions This surgical strategy is safe and effective for SHP not benefited from medical therapy.

OBJECTIVE:To observe the efficacy and safety of adjuvant Shensong Yangxin Capsule(SYC)in patients with stable angina(SA)receiving conventional therapy.METHODS:Fifty-eight chronic stable angina(CSA)patients were randomly assigned to 2 groups:the SYC group(n=28)treated with SYC plus conventional therapy while the control group(n=28)with conventional therapy alone.The patients were allowed to take nitroglycerine if number of the angina attacks ≥ 2 per day.The course of treatment fro both groups was 12 weeks.RESULTS:The total ischemic time during 24 hours,the ST segment decreasing amplitude and the frequency of angina pectoris attacks in two groups were all improved significantly after treatment(P

Objective To explore the clinical and electrophysiological characteristics of diabetic lumbosacral radiculoplexus neuropathy (DLRPN). Methods The clinical, electrophysiological and neuroimaging changes in 15 cases of DLRPN were investigated. Results The major clinical manifestations of 13 cases were unilateral or asymmetrical pain and progressive muscular weakness and atrophy of the proximal lower limb. The nerve conduction studies in affected nerves showed absent or reduced compound muscle action potential amplitudes and sensory responses with proportionate slowing of the conduction velocities. F waves and H reflexes were of long latencies or absent. Electromyography of affected muscles showed positive sharp waves and fibrillation potentials involving lumbar paraspinal muscles. Assessment of the motor unit action potentials (MUAPs) revealed high amplitude, long duration, and polyphasic MUAPs with reduced recruitment 50% cases have much lower R-R interval variation. Conclusions DLRPN presents disabling pain and muscular weakness and elctrophysiological examination has a paramount value in the diagnosis and evaluation of this disease

We report the results of nephrectomy under laparoscopy for 3 patients. Three patients were subjected to laparoscopy for the treatment of hydronephrosis of no function under general aneshesia. Succesiful operation was performed in the 3 patients without complication, the average operation time being 240 minutes, and hospitelization duration averaging 4.5 days.

AIM: To review variety of astragloside Ⅳ in Radix Astragali of different sources and different processed products. METHODS: HPLC-ELSD was used to determine astragloside Ⅳ in Radix Astragli. RESULTS: The distinction of astragloside Ⅳ in Radix Astragali of different sources and different processed products was obvious. The astragloside Ⅳ content reduced after processing. CONCLUSION: Radix Astragli of Henan Province has the highest astragloside Ⅳ content. And it is suggested that crude medicine should be used when astragloside Ⅳ is used as therapeutic component.