Objective To investigate the therapeutic effect and mechanism of ultrashort-wave therapy combined with electroacupuncture on the experimental rabbits with knee osteoarthritis.Methods Ten of fifty-five experimental Japanese big-ear rabbits were randomized into the blank control group, and the other 45 were made knee osteoarthritis models by prolonged fixation of the right posterior knee joint in extension for 6 weeks. The 40 successfully made models were randomized into model control group, electroacupuncture group, ultrashort-wave group, and combined electroacupuncture and ultrashort-wave group, 10 in each. The electroacupuncture group was treated with electroacupuncture at Xuehai (SP 10), Liangqiu (ST 34), Yanglingquan (GB 34), and Zusanli (ST 36) of the right posterior leg, the ultrashort-wave group was treated with ultrashort-wave therapy, and the combined electroacupuncture and ultrashort-wave group was treated with electroacupuncture and ultrashort-wave therapy, each time 25min, once a day. After treatment for 30 days, the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and nitric oxide (NO) in serum and the movement of affected knee joint were measured respectively in each group. Results Compared with the model control group, there was a significant increase of SOD, decrease of NO and MDA and knee movement improvement in the electroacupuncture, ultrashort-wave, and combined groups (P

Objective To observe the effect and adverse reaction of sufentanil combined with lornoxicam in venous controlled analgesia after hip arthroplasty. Methods Fifty patients with general anesthesia orthopaedic hip arthroplasty were randomly divided into sufentanil group (group S) and finnan fluoride group ( F) , group S sufentanil 2μg/kg,lornoxicam 40mg, physiological saline diluted to 100ml canister, Group F fentanyl 20μg/kg, lomoxicam 40mg, physiological saline diluted to 100ml canister. After operation connected intravenous infusion pump analgesia. Results Group S postoperative analgesia was obviously better than the group F,48h abnormal sweating and nausea and vomiting incidence was less than the group F( all P ＜ 0.05). Conclusion The effect of sufentanil combined lornoxicam for hip arthroplasty after intravenous analgesia was better than that of fentanyl combined lornoxicam group and had fewer side effects.

Objective To observe the effect of postoperative analgesia of flurbiprofen for percutaneous vertebral angioplasty in preemptive analgesia. Methods 40 paifients with ASA Ⅰ - Ⅱ level elective through skin vertebral angioplasty resection patients were seleted and randomly divided into 40 cases, flurbiprofen preemptive analgesia group(group A), and flurbiprofen postoperative analgesia group (group B). Group A was administrated with 50mg flurbiprofen before operation through intravenous, and Group B was administrated with it in the end of operation. By visual analog scale(VAS) ,the score of postoperative 0,1,4,8 and 12h level of pain and postoperative analgesia 24h overall evaluation was assessed. And the additional analgesics and deelop aderse reactions of patients were recorded.Results After operation 24h overall VAS score of group B (1.8 + 0.6) points was above that of group A (3.2 +0. 8) points (P ＜ 0.05). Both groups had no obvious adverse reactions. Conclusion Postoperative analgesia of flurbiprofen for pereutaneous vertebral angioplasty in preemptive analgesia had exact effect,convenience and less adverse reactions.

Enforcing microbiology teaching and helping students gain comprehensive knowledge on microbiology have always been the goal for the teachers concerned,since microbiology plays an important role both in theory and in practical life.In microbiology teaching,how to stim- ulate students' interest is crucial to the teaching aim.This article mainly argues four items such as encouraging students participate in teach- ing,employing multimedia,optimizing contents of the textbook and integrating theory,and practice in that teaching.

Objective:To develop a domain-specific perfectionism scale for college students.Methods:Exploratory and confirmatory factor analytic approaches were used to develop and evaluate the intra-structure of the scale.Results:Exploratory principle factor analysis of the items indicated that the scale had five factors,explaining 57.538% of the total variance.Internal consistency analysis showed that the Cronbach's ? coefficient,split-half and test-retest correlation coefficients were 0.648～0.781,0.664～0.790,0.720～0.779 respectively for the factors.Confirmatory fator analysis showed that the fit indexes for ?2/df,GFI,AGFI,NFI,NNFI,RMSEA,CFI,IFI were 2.32,0.91,0.88,0.84,0.88,0.06,0.90,0.90.Conclusion:Psychometric properties analysis shows that the scale achieves from good to exellent levels of internal consistency,test-retest rliability and content,construct and criteria-related validity.

Objective: To explore the mediation of stress between perfectionism and life satisfaction.Methods: 317 college students were surveyed with questionnaires.The data were analyzed by hierarchical regression.Results: ①Regarding general perfectionism,both male and female students' stress fully mediated negative perfectionism and life satisfaction but did not mediate positive perfectionism and life satisfaction;②Regarding domain-specific perfectionism,male students' appearance stress fully mediated related perfectionism and satisfaction,but for female students its mediation was partial.Both male and female students' personality stress fully mediated personality perfectionism and satisfaction.Mediation models involved academic,love,interpersonal relationship perfectionism and their related satisfaction were not found.Conclusion: The mediations of stress between perfectionism and life satisfaction are different in different life domains.

Objective: To explore the reliability and validity of Ruminative Responses Scale in Chinese version. Methods: A total of 912 Chinese college students completed the RRS. Results: All the indexes of confirmatory analysis including ?2、RMSEA、GFI、AGFI、NNFI、CFI、IFI met the requirements of psychological assessment. The ? coefficients of the scale and subscales and test -retest reliabilities were over 0.63. There was gender difference in rumination level. Conclusion: RRS is appropriate for the assessment of rumination in Chinese colleges.

Objective:To develop an appearance-management behavior scale for college female students.Methods:Based on literature analysis and open questionnaire,the preliminary scale was formed.A total of 822 college female students completed the scale.Exploratory and confirmatory factor analytic approaches were used to develop and evaluate the intra-structure of the scale.Results:Exploratory principle factor analysis of the items indicated that the scale had three factors.Internal consistency analysis showed that the Cronbach' ? coefficient,split-half and test-retest correlation coefficients were 0.88,0.86,0.77 respectively.Confirmatory factor analysis showed that the fit indexes for ?2/df,GFI,AGFI,NFI,NNFI,RMSEA,CFI,IFI were 3.20,0.87,0.83,0.82,0.85,0.085,0.87,0.87.Total scale had no significant correlation with SAAS,but with APS,PSS,IMS,SES (r=0.23,0.21,0.38,0.28,P

Objective To compare the biocompatible and osteconduetian behavior of the high-density porous polyethylene and hydroxyapatite/ultra-high molecular weight polyethylene. Methods Osteoectomy of the upper orbital rim was performed on 60 adult Japan rabbits. The animals were randomly divided into 3 groups with 20 of each. The hydroxyapatite/ultra-high molecular weight polyethylene, high-density porous polyethylene was implanted to the upper orbital defect of the animals in respective group. Animals were sacrificed at the 1st, 2nd, 4th and 8th weeks. Alkaline phosphatase (ALP) and Osteocalcin (OC)in the HDPE and hydroxyapatite/ultra-high molecular weight polyethylene was also detected. Result During the experimental period of 8 weeks, there was no implants extrusion or displacement. Fibrous tissue and new vessels grew into HDPE were obviously elder than those grew into hydroxyapatite/ultra high molecular weight polyethy-lene. Alkaline phosphatase and osteocalcin contents has great difference, HDPE group is the best, followed by HA combination artificial group (P<0.05). Conclusions High-density porous polyethylene and hydroxyapatite/ultra-high molecular weight polyethylene are both good orbital reconstruction materials, they all have good biocompatiblity and osteoconductian, but high-density porous polyethylene is better than hydroxyapatite/ultra-high molecular weight polyethylene in osteoconductian.

Objective To improve the current method of spermatogonial cells transplantation, and make the new method become more operative and easy to carry out. Methods The spermatogonial cells in rC57BL6/tg14 (act-EGFO-Osb Y01) mice that expresse GFP protein were collected as the donor cells, and by using a modified syringe, and then were injected into the seminiferous tubules of pretreated wild type GFP-null mice through testicular efferent duct. The transplantation outcome was evaluated by trypan blue stainting and fluorescent microscopic examination and pathohistological analysis of transplanted testis. Results The transplanted testis of recipient mice showed green fluorescence signal, and the signal of seminiferous tubules was found significantly higher than that of the surrounding tissues. The transplanted GFP-positive cells generated colonies and spermatogenesis but pretreated wild type GFP-null mice were not found. Conclusion The expressed GFP protein spermatogonial cells in rC57BL6/tg14 mice were successfully transplanted in the wild type GFP-null recipient mice, fourthermore the improved transplantation method simplified the triditional one and achieved the same transplantation results.