Objective To observe the effect and adverse reaction of sufentanil combined with lornoxicam in venous controlled analgesia after hip arthroplasty. Methods Fifty patients with general anesthesia orthopaedic hip arthroplasty were randomly divided into sufentanil group (group S) and finnan fluoride group ( F) , group S sufentanil 2μg/kg,lornoxicam 40mg, physiological saline diluted to 100ml canister, Group F fentanyl 20μg/kg, lomoxicam 40mg, physiological saline diluted to 100ml canister. After operation connected intravenous infusion pump analgesia. Results Group S postoperative analgesia was obviously better than the group F,48h abnormal sweating and nausea and vomiting incidence was less than the group F( all P ＜ 0.05). Conclusion The effect of sufentanil combined lornoxicam for hip arthroplasty after intravenous analgesia was better than that of fentanyl combined lornoxicam group and had fewer side effects.

Objective To observe the effect of postoperative analgesia of flurbiprofen for percutaneous vertebral angioplasty in preemptive analgesia. Methods 40 paifients with ASA Ⅰ - Ⅱ level elective through skin vertebral angioplasty resection patients were seleted and randomly divided into 40 cases, flurbiprofen preemptive analgesia group(group A), and flurbiprofen postoperative analgesia group (group B). Group A was administrated with 50mg flurbiprofen before operation through intravenous, and Group B was administrated with it in the end of operation. By visual analog scale(VAS) ,the score of postoperative 0,1,4,8 and 12h level of pain and postoperative analgesia 24h overall evaluation was assessed. And the additional analgesics and deelop aderse reactions of patients were recorded.Results After operation 24h overall VAS score of group B (1.8 + 0.6) points was above that of group A (3.2 +0. 8) points (P ＜ 0.05). Both groups had no obvious adverse reactions. Conclusion Postoperative analgesia of flurbiprofen for pereutaneous vertebral angioplasty in preemptive analgesia had exact effect,convenience and less adverse reactions.

Enforcing microbiology teaching and helping students gain comprehensive knowledge on microbiology have always been the goal for the teachers concerned,since microbiology plays an important role both in theory and in practical life.In microbiology teaching,how to stim- ulate students' interest is crucial to the teaching aim.This article mainly argues four items such as encouraging students participate in teach- ing,employing multimedia,optimizing contents of the textbook and integrating theory,and practice in that teaching.

Objective To investigate the therapeutic effect and mechanism of ultrashort-wave therapy combined with electroacupuncture on the experimental rabbits with knee osteoarthritis.Methods Ten of fifty-five experimental Japanese big-ear rabbits were randomized into the blank control group, and the other 45 were made knee osteoarthritis models by prolonged fixation of the right posterior knee joint in extension for 6 weeks. The 40 successfully made models were randomized into model control group, electroacupuncture group, ultrashort-wave group, and combined electroacupuncture and ultrashort-wave group, 10 in each. The electroacupuncture group was treated with electroacupuncture at Xuehai (SP 10), Liangqiu (ST 34), Yanglingquan (GB 34), and Zusanli (ST 36) of the right posterior leg, the ultrashort-wave group was treated with ultrashort-wave therapy, and the combined electroacupuncture and ultrashort-wave group was treated with electroacupuncture and ultrashort-wave therapy, each time 25min, once a day. After treatment for 30 days, the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and nitric oxide (NO) in serum and the movement of affected knee joint were measured respectively in each group. Results Compared with the model control group, there was a significant increase of SOD, decrease of NO and MDA and knee movement improvement in the electroacupuncture, ultrashort-wave, and combined groups (P

ObjectiveTo explore the efficacy of large trauma craniotomy with bilateral frontal coronal incision in treating contusion and laceration of bilateral frontal lobes.MethodsThe clinical data of 68 patients with contusion and laceration of bilateral frontal lobes who were treated with bilateral decompressive craniectomy were analyzed retrospectively.There were 36 cases(observation group) treated with large trauma craniotomy with bilateral frontal coronal incision and 32 cases (control group) given bilateral decompressive craniectomy by stages.The prognosis of two groups were observed and compared.The prognosis was evaluated at 6 months after surgery by Glasgow outcome scale (GOS) score.ResultsThere were 23 cases (63.89％,23/36) who got good recovery,8 cases(22.22％,8/36) with poor prognosis and 5 dead cases (13.89％,5/36) in observation group.There were 11 cases (34.38％,11/32) who got good recovery,9 cases (28.12％,9/32) with poor prognosis and 12 dead cases (37.50％,12/32) in control group.The rate of good recovery and mortality between two groups had significant differences (P ＜ 0.05).ConclusionsThe large trauma craniotomy with bilateral frontal coronal incision can significantly relieve or ease intracranial hypertension of patients with contusion and laceration of bilateral frontal lobes.And it can improve the prognosis and decrease the mortality.

Objective To study the influence on T-lymphocyte subsets in herpes zoster with adjuvant treatment of compound glycyrrhizin.Methods 93 patients with herpes zoster were randomly divided into two groups:control group(46 cases,treated with antiviral drugs) and 1 treatment group(47 cases,treated with compound glycyrrhizin and antiviral drugs).The expression of T-lymphocyte subsets on the T cell surface in patients and control group were determined by flow cytometry.Results The expression of CD3,CD4,CD8,CD4/CD8 ratio was significantly different in the treatment group and control group.Conclusion Compound glycyrrhizic adjuvant therapy could not only shorten check blister,acetanilide,scar tissue and recovery time,reduce the sequela of neuralgia,but also affect the expression state of T lymphocyte subsets with herpes zoster,improve the cellular immune function with herpes zoster.

Objective To improve the current method of spermatogonial cells transplantation, and make the new method become more operative and easy to carry out. Methods The spermatogonial cells in rC57BL6/tg14 (act-EGFO-Osb Y01) mice that expresse GFP protein were collected as the donor cells, and by using a modified syringe, and then were injected into the seminiferous tubules of pretreated wild type GFP-null mice through testicular efferent duct. The transplantation outcome was evaluated by trypan blue stainting and fluorescent microscopic examination and pathohistological analysis of transplanted testis. Results The transplanted testis of recipient mice showed green fluorescence signal, and the signal of seminiferous tubules was found significantly higher than that of the surrounding tissues. The transplanted GFP-positive cells generated colonies and spermatogenesis but pretreated wild type GFP-null mice were not found. Conclusion The expressed GFP protein spermatogonial cells in rC57BL6/tg14 mice were successfully transplanted in the wild type GFP-null recipient mice, fourthermore the improved transplantation method simplified the triditional one and achieved the same transplantation results.

Objective To investigate the levels of inflammatory cytokines in children with low absolute lymphocyte count (ALC).Methods 56 patients with low ALC as the patients group and 20 children with healthy physical examination as the control group were enrolled.Several inflammatory cytokines,namely IL-1,IL-6,IL-8 and TNF-βwere measured with enzyme linked immu-nosorbent assay(ELISA)kits.High sensitive C reactive protein(Hs-CRP)was measured by the immunoturbidimetry(ITM)kit with the automatic biochemical analyzer.Results The concentrations of IL-1,IL-6,IL-8 and TNF-βin the patients were(0.096±0.012) ng/mL,(0.118±0.026)ng/mL,(0.388 ±0.069)ng/mL and(1.256 ±0.245 )ng/mL respectively,which had no statistical differ-ences as compared with the control group(P >0.05);the concentration of HsCRP in the patients group was(5.7±2.8)mg/L,while which in the control group was(4.8±2.6)mg/L,showing no statistically significant difference between them(P >0.05).Conclusion The levels of the cytokines in low ALC patients are not increased,which may be related with the other factors.Actively using tra-ditional Chinese medicine treatment is beneficial to the recovery of disease.

Objective To explore the feasibility and accuracy of dual-source CT (DSCT)in evaluating aortic regurgitation (AR). Methods Among 78 patients who underwent both DSCT and TTE,38 patients were diagnosed as AR and 40 without AR served as controls.Maximum regurgitant orifice area (ROA)in diastole was planimetrically measured with DSCT,and measurements were compared with semiquantitative classification of TTE.Results DSCT correctly identified 37 patients with AR.Compared with TTE,two patients were false positive and three patients were false negative findings by DSCT (sensitivity 92%,specificity 95%, positive predictive value 94%,and negative predictive value 93%).ROA measured by DSCT (0.32 cm2 ±0.44 cm2 )was signifi-cantly correlated with TTE classification (r=0.85,P<0.01).With receiver operating characteristic curve analysis,discrimination between degrees of AR with DSCT was high accurate when using cutoff ROAs of 0.08 cm2 and 0.58 cm2 .Conclusion Planimetric measurement of ROA with DSCT is accurate for the quantitative evaluation of AR.

Purpose: To investigate the therapeutic effect of acupuncture and herbs in chloasma.Methods: Chloasma was divided into three patterns and treated by acupuncture plus oral administration and external application of Chinese herbs. Results: There was an obvious therapeutic effect in chloasma treated by combination of acupuncture and herbal drugs. After two courses of the treatments, the effective rate accounted for 93.3% in 45 cases. Conclusion: There was an obvious therapeutic effect in chloasma treated by combination of acupuncture and herbal drugs.