1.Impacts of different stimulation cycles on outcomes of intrauterine insemination
Changying XING ; Hongfang SHAO ; Lu FANG ; Jiang BIAN ; Lihong WANG ; Minfang TAO
The Journal of Practical Medicine 2015;(6):933-936
Objective To explore the impacts of natural ovulation cycles and stimulation cycles on the outcome of intrauterine insemination (IUI) in order to improve the clinical effects of IUI. Methods 176 women received 384 stimulation cycles. According to different ovulation stimulation protocols , the women were divided into six groups including natural cycle (NC) group, clomiphene citrate (CC) group, letrozole (LE) group;human menopausal gonadotrophin (HMG ) group, CC + HMG group, and LE + HMG group. The pregnancy rate between nature cycles and ovarian hyperstimulation cycles was compared. Results The pregnancy rate was 9.33%in the nature cycle group and 13.27% in the stimulation cycle group, with a significant difference (P < 0.05);and it dif not differ significantly among the stimulation cycle groups (P > 0.05). Conclusions Use of ovulation-induction medications is one of the important factors affecting the pregnancy rate of intrauterine insemination. There are no differences in the outcome of IUI among different ovulation stimulation protocols.
2.Quantitative assessment of ischemic liver's elastic changes in rabbits using acoustic radiation force impulse (ARFI)
Li WANG ; Pintong HUANG ; Huiliao HE ; Fanlei KONG ; Zhaoling LU ; Zhilan ZHENG ; Zongmin WANG ; Hongfang WU ; Zhequn LI
Chinese Journal of Ultrasonography 2010;19(11):989-992
Objective To evaluate the change of elasticity of rabbit liver following portal inflow vessels occlusion using acoustic radiation force impulse (ARFI). Methods Twelve Japanese white big ear rabbits were used to establish total hepatic vascular exclusion animal models by occluding the portal inflow vessels. The elasticity of rabbits' right liver was detected during the stages of baseline(T0), 5 min(T1),20min(T2) ,30min (T3)after occluding the portal inflow vessels using ARFI respectively. The elasticity of rabbit liver, levels of serum's aspartate aminotransferase (AST), alanine aminotransferase (ALT) and historical results of hepatic tissue during different stage were compared. Results Shear wave velocity(Vs)of rabbits' right livers in T2 was significantly higher than that in T0 ( P <0.05) and Vs of rabbits' right livers in T3 was significantly higher than that in T2 ( P <0.05). No significant differences of serum's AST,ALT levels between T2 and T0 were found,while the Vs of rabbit live in T2 was slightly higher than that in T0 (P>0.05), serum's AST, ALT levels in T3 were significantly higher than that in T2 ( P <0.05).Historical changes of liver ischemia and injury were observed in T2 and T3 with light microscopy. Conclusions The liver ischemia and injury severity can be accurately and objectively evaluated using ARFI.
3.Idenfication of microRNAs profiles in nasopharyngeal carcinoma.
Guohui NIE ; Feng LIU ; Yilin LIN ; Jing CHEN ; Zhendong YU ; Xiaoqing LI ; Hongfang DUAN ; Ruijing LU ; Chunjuan ZHAO ; Hongyi HU ; Xueshuang MEI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(13):975-978
OBJECTIVE:
To filtrate and prove the different microRNAs (miRs) profiles in nasopharyngeal carcinoma.
METHOD:
Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis. Then we used RT-QPCR technology to prove the accuracy of screening results.
RESULT:
There were significant expression differences of miRs between nasopharyngeal carcinoma and the control tissues, 144 human miRs had 2 or more fold the difference ratio. Compared with the inflammatory tissues, we have found that miRs-34b, miRs-449b and miRs-7-1 significantly low expressed in nasopharyngeal carcinoma, yet miRs-125b, miRs-184, miRs-196b, miRs-205 and miRs-24-1 expressed high. The results were consistent with the microarray analysis.
CONCLUSION
The difference expressed miRs might be closely related to the process of nasopharyngeal carcinoma, and the research on miRs profiles maybe provide a powerful target basis for early diagnosis and therapy of nasopharyngeal carcinoma.
Carcinoma
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Gene Expression Profiling
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Humans
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MicroRNAs
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genetics
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Nasopharyngeal Carcinoma
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Nasopharyngeal Neoplasms
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genetics
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Oligonucleotide Array Sequence Analysis
4.Expression of SP1 in esophageal squamous cell carcinoma and its effect on proliferation and apoptosis of esophageal squamous cell carcinoma cells
Hongfang HUANG ; Xiangyun LU ; Mengfei SUN ; Xiaobin CUI
Acta Universitatis Medicinalis Anhui 2023;58(12):2031-2037
Objective To investigate the expression of Specific protein1(SP1)in esophageal squamous cell carci-noma(ESCC)and adjacent normal tissues and its effect on the proliferation and apoptosis of ESCC cells.Methods The expression of SP1 protein in 121 ESCC tissues and 74 adjacent normal tissues was detected by immunohisto-chemistry.Chi-square test and Cox regression analysis were used to analyze the relationship between SP1 and clini-copathological parameters and survival prognosis of ESCC patients.SP1 siRNA(small interfering RNA)was con-structed and transfected into esophageal squamous cell carcinoma Eca1 09 and EC9706 cell lines.Western blot was used to detect the expression of SP1 after transfection.The effects of SP1 on the proliferation and apoptosis of e-sophageal squamous cell carcinoma cells were detected by cloning assay,CCK-8 cell proliferation assay and flow cytometry.Results SP1 protein was expressed in the nucleus of esophageal squamous cell carcinoma tissues,and the expression rate of SP1 protein in esophageal squamous cell carcinoma tissues was significantly higher than that in adjacent normal tissues(x2=20.568,P<0.01).Comparison between groups showed that the high expression rate of SP1 was higher in female(P=0.041),moderately or poorly differentiated(P=0.038)and T3-T4 inva-sion depth(P=0.041)ESCC(esophageal squamous cell carcinoma)patients.Log-rank test showed that the sur-vival time of patients with high expression of SP1 was shorter than that of patients with low expression of SP1(P=0.048).Multivariate Cox regression analysis showed that TNM(tumor node metastasis classification)stage(Ⅲ+Ⅳ)was a potential risk factor for shorter survival time in patients with esophageal squamous cell carcinoma(P<0.001).Cell biological experiments showed that compared with the control group,the proliferation ability of esoph-ageal squamous cell carcinoma cell lines decreased(P<0.05)and the apoptosis index increased(P<0.05)after silencing SP1.Conclusion SP1 protein is highly expressed in human esophageal squamous cell carcinoma tissues and is associated with poor prognosis in patients.Silencing SP1 can inhibit the proliferation of esophageal squamous cell carcinoma cells and promote their apoptosis.
5.Advances in the 8th Japan-China Hepato-Pancreato-Biliary Symposium
Rui TANG ; Liang WANG ; Xuedong WANG ; Shiwei YANG ; Xiaobin FENG ; Jun YAN ; Canhong XIANG ; Yuewei ZHANG ; Hongfang YIN ; Qian LU ; Jiahong DONG
Chinese Journal of Digestive Surgery 2018;17(12):1166-1175
The 8th Japan-China Hepato-Pancreato-Biliary Symposium was held in Tokyo,Japan from 22nd to 23rd November 2018.The meeting was convened coincidently with the 80th Annual Congress of Japanese Surgical Association,which attracted the participation of Chinese and Japanese hepatobiliary and pancreatic surgeons.The symposium aimed to explore the latest achievements and clinical issues of diagnosis and treatment for hepato-pancreato-biliary diseases.In this article,authors reviewed the up-to-date research information in order to share the experience,achievements and new information in the field of hepatobiliary and pancreatic diseases with colleagues.
6.ICU nurses' needs analysis of employee assistance program
Jinlin FANG ; Hongfang YE ; Yan CHEN ; Lu CHEN ; Min LI ; Huanhuan ZHU ; Zheyun WANG ; Yuanyuan YAO ; Cuiling JI ; Jin TAO
Chinese Journal of Practical Nursing 2021;37(24):1897-1901
Objective:To understand the current status of nurses' awareness and needs for the Employee Assistance Program (EAP), as to provide basis for the hospital to formulate EAP.Methods:Using the purpose sampling method, 352 ICU nurses from the Nanjing Drum Tower Hospital Affiliated of Nanjing University Medical School were selected as the research subjects from December 2019 to January 2020. Finally, 350 valid questionnaires were obtained. Use the EAP demand questionnaire to investigate the ICU nurses' awareness and demand for EAP.Results:Among the 350 nurses in the intensive unit, 214 cases (61.14%) had never understood EAP, only 8 (2.29%) had understood and experienced the EAP, and 332 cases (94.86%) believed that the hospital′s implementation of EAP was a benefit provided to nurses. The top two service requirements were: "Relief of work pressure" and "mental health consultation", and hope to receive mixed-mode EAP services; in ICU and CCU, nurses hope EAP can help resolve work-family conflicts; in EICU, they need to provide Specific job skills training services.Conclusions:ICU nurses have insufficient cognition of EAP and urgently need to obtain EAP implementation knowledge; at the same time, it is necessary to formulate an implementation plan that meets ICU nurses′ needs for EAP, that is, to relieve work pressure and carry out psychological counseling to ensure that EAP is launch successfully in ICU nurses.
8.High-risk phenotypes of genetic disease in a Neonatal Intensive Care Unit population.
Tiantian XIAO ; Qi NI ; Huiyao CHEN ; Huijun WANG ; Lin YANG ; Bingbing WU ; Yun CAO ; Guoqiang CHENG ; Laishuan WANG ; Liyuan HU ; Hongfang MEI ; Yulan LU ; Mengchun GONG ; Xinran DONG ; Wenhao ZHOU
Chinese Medical Journal 2022;135(5):625-627