ObjectiveTo establish a rapid,sensitive and accurate method to detect Mycoplasma genitalium,and to evaluate the prevalence of M.genitalium among unlicensed prostitutes from Hezhou city in Guangxi Zhuang Autonomous Region.MethodsA pair of primers and Taqman MGB probe were designed and synthesized for the Pa gene of M.genitalium.Standard samples were prepared with the M.genitalium type strain G37.The established Taqman MGB real time fluorescence-based PCR assay was used to detect M.genitalium in the standard samples and cervical swab specimens collected from unlicensed prostitutes in Hezhou city of Guangxi Zhuang Autonomous Region.ResultsThe established Taqman MGB real time PCR exhibited a wide linear range( 1 × 10 copies/μl to 1 × 106 copies/μl,R2 =0.993),good repeatability(intra-assay variation;0.7％,inter-assay variation:1.09％) and hign sensitivity with the limit of detection being 10 copies/μl and limit of quantification being 50 copies/μl.As the assay showed,12.1％ of the 404 cervical swab samples were positive for M.genitalium.ConculsionThe Taqman MGB real time fluorescence-based PCR is a rapid and sensitive method for the quantitative and qualitative detection of M.genitalium.

Objectives To investigate the imaging ability of magnetic sensitive weighted imaging (SWI) scans with 2.0 mm and 1.0 mm slice thickness on intracerebral veins and branches.Methods 47 healthy volunteers were recruited,including 20 males and 27 females,aged 21-45 (30.1 ± 12.93).Two SWI sequences with thickness of 2.0 mm and 1.0 mm were performed in all healthy volunteers.After reconstructed by minimal intensity projection (MIP),the display rate and diameter of intracerebral veins and branches were counted by two doctors.McNemar test and paired t-test were used for statistical analysis.Results (1) The display rates of bilateral internal cerebral veins,papular veins and left ventricular veins were 100％ in both scanning schemes,while the display rates of left and right septal veins,anterior caudate nucleus veins and right ventricular veins on SWI sequence images with 2.0 mm thickness were 90.0％ (left),91.0％ (right) and 87.2％ (right),91.5％,respectively.The display rates on SWI sequence images with 1.0 mm thickness were 97.9％ (left),94.0％ (right),97.9％ (left),95.7％ (right) and 95.7％ respectively.(2) There were significant differences in the diameter of bilateral septum pellucidum veins and bilateral anterior caudate nucleus veins between the two scanning schemes (P ＜ 0.05).While there was no significant difference in the diameter of bilateral internal cerebral veins,bilateral papular veins and bilateral intraventricular veins (P ＞0.05).(3) The displaying rate of veins with diameter ＞0.9 mm was 100％ for both scanning schemes,while the displaying rate of veins with diameter ranging from 0.6-0.9 mm (including 0.6 mm and 0.9 mm) in the two scanning schemes had significant difference (P ＜ 0.05).Conclusions SWI sequence can clearly show the anatomical shape of intracerebral veins and branches.In contrast to SWI of the scan slices thickness of 2.0 mm,the SWI of scan slices thickness of 1.0 mm showed more efficiency in display thinner brain venous.Therefore,using SWI sequence scanning scheme with 1.0 mm slice thickness will be more conducive to observe and study intracerebral veins and branches.

Objective In this study,we attempted to investigate whether Electroacupuncture(EA)could promote the autophagy function in macrophages of inflammatory skin tissues by activating CB2 receptor,thus relieving inflammatory pain induced by CFA in mice,and whether activation of CB2 receptor in NR8383 macrophages cell line can simulate the effect of EA on the autophagy function and mitochondrial damage.Methods Inflammatory pain model was induced by CFA injection into the planta the hind paw of wildtype and CB2 knockout mice.EA or sham EA was applied on the left Huantiao(GB30)and Yanglingquan(GB34)sites.Thermal hyperalgesia was determined with the Hargreaves test.Mechanical sensitivity was assessed with von Frey filaments.NR8383 microphage cell line was used to study the effect of CB2 activation on macrophage function induced by CFA.The expression level of autophagy protein LC3 and p62 in wildtype and CB2 knockout mice skin tissue and NR8383 cell line were determined by Western blot.And flow cytometry analysis was applied to detect damaged mitochondria and mitochondrial superoxide.Results CFA significantly reduced the thermal and mechanical pain threshold in both wildtype and CB2 knockout mice,comparing with the vehicle control groups(P<0.01).EA significantly inhibited thermal and mechanical hyperpathia induced by CFA in wildtype mice(P<0.05),but had no effect on CB2 knockout mice with CFA(P>0.05).CFA significantly increased the expression of p62 protein and decreased LC3-II/I ratio,which was inversed by EA in wildtype mice but wasn't affected by EA in CB2 knockout mice.CFA increased the expression of p62 protein and decreased LC3-II/I ratio in NR8383 cell line,which were inversed by CB2 agonist AM1241.CFA increased mitochondria damage,which were then attenuated by CB2 agonist AM1241.Conclusion The analgesic effect of EA on inflammatory pain induced by CFA was mediated by activation of CB2 receptor,which promoted the autophagy function and the clearance of damaged mitochondria in macrophage.

Objective:To explore the mediating effect of work motivation of clinical nurses between work-family conflict and invisible absence, so as to provide a basis for reducing nurses′ invisible absence.Methods:From September to December 2022, a cross-sectional survey was conducted to investigate the mediating effect of work motivation between the work-family conflict and invisible absence by using the general information questionnaire, Work-Family Conflict Scale, Work Motivation Scale and Stanford Invisible Absence Scale among 522 nurses by convenience sampling.Results:The valid questionnaires were 511 samples. There were 15 male nurses and 496 female nurses among the 511 nurses. The work-family conflict of clinical nurses had a positive effect on invisible absence ( r=0.319, P<0.05), and work motivation had a partial mediating effect between work-family conflict and invisible absence (the effect value was 0.082), the mediating effect accounted for 35.00% of the total effect. Conclusions:Nursing managers and nurses themselves should take a variety of ways to reduce the work-family conflict, promote the work-family balance of nurses, and improve their work motivation, so as to reduce their invisible absence and promote the healthy development of clinical nurses.