Objective:To explore the clinical significance of prealbumin(PA) and γ-glutamyltranspeptidase (γ-GT) detection in evaluation of hyperbilirubinemia in neonates at different stages.Methods:From August 2017 to August 2018, 300 full-term delivery patients with neonatal hyperbilirubinemia were selected, including 210 early-stage neonates and 90 late-stage neonates.According to the severity of bilirubinemia, the patients were classified into mild group (50 cases), moderate group (150 cases), and severe group (100 cases). The blood levels of PA and γ-GT of each group were detected.Results:The levels of PA and γ-GT in late neonates were (95.81±4.58)mg/L, (44.97±5.21)IU/L, respectively, which were significantly higher than those in early neonates [(94.77±6.32)mg/L, (53.88±6.32)IU/L]( t=1.410, 11.767, P=0.160, 0.000). With the increase of bilirubin level, the blood PA level was gradually decreased( P<0.05). The blood γ-GT level of moderate and severe patients were significantly higher than that of mild ones ( t=2.222, 2.020, P=0.027, 0.046). The blood levels of γ-GT and PA had no statistically significant differences between moderate patients and severe patients ( t=0.712, 1.741; P=0.477, 0.083). The blood PA level of moderate and severe patients were significantly lower than that of mild patients ( t=2.357, 3.277, P=0.019, 0.001). The serum PA levels had no statistically significant difference between severe patients and moderate patients ( t=0.719, P=0.474); and the serum PA levels of severe and moderate patients were lower than that of mild patients ( t=3.234, 2.117, P=0.001, 0.043). The serum γ-GT levels among the three groups had no statistically significant differences (severe vs.moderate: t=0.297, P=0.767; severe vs.mild: t=0.269, P=0.788; moderate vs.mild: t=0.013, P=0.989). Conclusion:By detecting the levels of PA and γ-GT in neonatal hyperbilirubinemia in different periods, it can provide a reference for clinical judgment of the condition of the children, thus guiding clinical rational treatment.