1.The effects of IL-24 on the differentiation and function of osteoclasts
Xin WANG ; Ce SHI ; Rong ZHENG ; Xuan LIU ; Hongchen SUN ; Lipeng WU
Journal of Practical Stomatology 2014;(4):456-459
Objective:To study the effect of IL-24 on the differentiation and function of osteoclasts.Methods:Mature osteoclasts were isolated from long bones of neonate rats.Optimal multiplicity of infection(MOI)of AdCMV-EGFP was determined.Then osteo-clasts and RAW264.7 cells were transfected with AdCMV-EGFP and AdCMV-IL-24 respectively.The function of osteoblasts was studied by the observation of bone resorption lacunae,the differentiation of RAW264.7 cells was evaluated by the expression of osteo-clast related genes examined with real-time PCR.Results:Osteoclasts were TRAP positive with more than 2 neclei.MOI=400 was suitable for AdCMV-EGFP transfection.The resorption lacunae area in AdCMV-IL24 transfected cells was larger than that in AdCMV-EGFP transfected cells(P<0.05).Real-time PCR showed that under induced conditions,osteoclastic related genes NFATc1,CTSK and TRAP were changed in RAW264.7 cells transfected with AdCMV-IL-24.Conclusion:IL-24 may promote the differentiation and function of osteoclasts.
2.Studies on the extraction of DNA using carboxyl-coated magnetic particles and quantification using fluorescent dye.
Yongjie MA ; Hongchen GU ; Weiming ZHENG
Journal of Biomedical Engineering 2007;24(5):1093-1096
Based on the study of extraction of DNA using carboxyl-coated Fe3O4, here we provide a rapid, high efficient, safe and economical method. Using fluorescent dye-Sybr Green I, the quantification of double-strand DNA exhibits the advantages of high sensitivity,good repeatability and convenience.
Carbon Dioxide
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chemistry
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DNA
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blood
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isolation & purification
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Ferric Compounds
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chemistry
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Fluorescent Dyes
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Humans
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Magnetics
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Nanoparticles
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Organic Chemicals
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Particle Size
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Staining and Labeling
3.Expression of Pichia pastoris tRNAProCCG and its function.
Meng PENG ; Ming TAN ; Yan ZENG ; Hongchen ZHENG ; Hui SONG
Chinese Journal of Biotechnology 2019;35(1):70-80
Translocation ribonucleic acid (tRNA) is one of the important components in protein synthesis. In order to explore the effect of the changes of tRNAs corresponding to rare codons (rarity tRNAs) on the expression of exogenous genes, the co-expression system of rare tRNA gene and exogenous gene in Pichia pastoris was constructed. The expression of GFP in P. pastoris can be greatly reduced when a repressor region composed of four continuous proline rare codon CCG was added into the GFP gene. The expression amount of the repressed GFP could be increased about 4.9% when tRNAProCCG gene was cointegrated to the 3' of the repressed GFP gene through pPIC9K to the genome of P. pastoris GS115. Meanwhile, the expression amount of the repressed GFP increased about 12.5% by integrating the repressed GFP gene and tRNAProCCG gene to the genome of P. pastoris GS115 through pPIC9K and pFLDα, respectively. Using the same method, NFATc3T-GFP fusion gene and tRNAProCCG gene were co-expressed in P. pastoris GS115 resulting in 21.3% increased of the expression amount of NFATc3T-GFP fusion protein. In conclusion, tRNAProCCG gene has been confirmed to be a kind of rare tRNAs in P. pastoris GS115. Through co-expression of tRNAProCCG gene and heterologous genes which containing the continuous rare codon CCG, the expression of the repressed heterologous genes could be increased significantly. Furthermore, this co-expression system would contribute to screening and determining the other rare tRNAs.
Codon
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Pichia
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Recombinant Proteins
4. Study regarding the parent-of-origin effect of WNT pathway genes on non-syndromic cleft lip with or without cleft palate among the Chinese population
Siyue WANG ; Mengying WANG ; Wenyong LI ; Ren ZHOU ; Hongchen ZHENG ; Dongjing LIU ; Nan LI ; Zhibo ZHOU ; Hongping ZHU ; Tao WU
Chinese Journal of Epidemiology 2019;40(6):670-675
Objective:
Non-syndromic cleft lip with or without cleft palate (NSCL/P) is a common birth defect with its genetic evidence widely explored. This study explored the potential the parent-of-origin (PoO) effect of WNT pathway on the risks of NSCL/P, using a case-parent trio design.
Methods:
Data on the single nucleotide polymorphism (SNP) of WNT genes were selected from a genome-wide association study (GWAS). A total of 806 Chinese non-syndromic cleft lip patients, with or without cleft palate (NSCL/P) case-parent trios, were gathered from an international consortium. PoO effect of WNT pathway genes and its haplotypes were explored by log-linear models. Additional
5.Changes of the World Health Organization 2022 classification (5th edition) of salivary glands tumors.
Kai Juan ZHENG ; Mei Si REN ; Chun Yan QIAO ; Dan Dan WANG ; Ming QU ; Yu Qing ZHANG ; Tong SHA ; Xiao Ling WANG ; Ce SHI ; Hongchen SUN
Chinese Journal of Stomatology 2022;57(11):1102-1112
Pathological diagnosis of salivary gland tumors is one of the most challenging areas in all head and neck surgical pathology. The classification of salivary gland tumors was updated in the 5th edition of the World Health Organization Classification of Head and Neck Tumours, most of which were based on their molecular pathological characteristerics. This new classification features a description of several new entitiesamong benign and malignant neoplasms, salivary gland tumors with updated naming or diagnostic criteria, and lesions deleted from this section, etc.This present review focuses on the updates and changes in the new classification of salivary gland tumors, and provides some reference for head and neck surgeons and pathologists.
Humans
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Head and Neck Neoplasms
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Salivary Gland Neoplasms/pathology*
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Salivary Glands
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World Health Organization
6.Outcomes of haploidentical bone marrow transplantation in patients with severe aplastic anemia-II that progressed from non-severe acquired aplastic anemia.
Hongchen LIU ; Xiaoli ZHENG ; Chengtao ZHANG ; Jiajun XIE ; Beibei GAO ; Jing SHAO ; Yan YANG ; Hengxiang WANG ; Jinsong YAN
Frontiers of Medicine 2021;15(5):718-727
Severe aplastic anemia II (SAA-II) progresses from non-severe aplastic anemia (NSAA). The unavailability of efficacious treatment has prompted the need for haploidentical bone marrow transplantation (haplo-BMT) in patients lacking a human leukocyte antigen (HLA)-matched donor. This study aimed to investigate the efficacy of haplo-BMT for patients with SAA-II. Twenty-two patients were included and followed up, and FLU/BU/CY/ATG was used as conditioning regimen. Among these patients, 21 were successfully engrafted, 19 of whom survived after haplo-BMT. Four patients experienced grade II-IV aGvHD, including two with grade III-IV aGvHD. Six patients experienced chronic GvHD, among whom four were mild and two were moderate. Twelve patients experienced infections during BMT. One was diagnosed with post-transplant lymphoproliferative disorder and one with probable EBV disease, and both recovered after rituximab infusion. Haplo-BMT achieved 3-year overall survival and disease-free survival rate of 86.4% ± 0.73% after a median follow-up of 42 months, indicating its effectiveness as a salvage therapy. These promising outcomes may support haplo-BMT as an alternative treatment strategy for patients with SAA-II lacking HLA-matched donors.
Anemia, Aplastic/therapy*
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Bone Marrow Transplantation
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Graft vs Host Disease
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HLA Antigens
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Hematopoietic Stem Cell Transplantation
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Humans
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Transplantation Conditioning