Chemotherapy is one of the primary treatments for cancer patients,while tumor multidrug re-sistance( MDR) represents a major obstacle in the treatments of patients,limiting the efficacy of chemotherapy.To date,the mechanism of drug resistance has not been fully elucidated.lncRNAs are a family of long noncoding RNAs that regulate gene expression by epigenetic modification,transcription and post transcriptional processing. Recent studies have showed that the aberrant regulation of target genes by lncRNAs may be involved in the acqui-sition of tumor MDR.Therefore,targeting lncRNAs may be an attractive strategy for improving the chemosentivity and reversing chemotherapeutic resistance.This paper reviews the relationship between lncRNAs and tumor drug resistance as well as the underlying possible mechanism.

OBJECTIVE To study the changes in trace elements Zn and Cr and cell immunologic function among elderly inpatients with lower respiratory tract infection.METHODS The changes in T-lymphocyte subpopulation and trace elements Zn and Cr in plasma and red blood cell of 52 inpatients with lower respiratory tract infection were compared with a control group of 53 healthy people of the same age group.The flame atom absorbing was for blood Zn testing,the non-flame atom absorbging for blood Cr testing the indirect immunofluorescent technique was for measurement of T cell subpopulation.RESULTS Among elderly inpatients with lower respiratory tract infection the ratio of Zn [(12.68?3.26)?mol/L] and Cr [(0.033?0.029)?mol/L] in plasma and Zn [(124.12?36.55)?mol/L] and Cr [(0.412?0.025)?mol/L] in RBC was significantly lower than control group [(17.39?4.78)?mol/L] and [(0.068?0.036)?mol/L],[(145.33?20.12)?mol/L] and [(0.666?0.038)?mol/L],respectively,the function of T-lympholyte subpopulation was reduced and in disorder,CD3 [(50.48?7.62)%],CD4 [(27.36?9.37)%] and CD4/CD8(0.67?0.23) was significantly lower than control group [(64.33?4.82)%,(42.35?8.12)% and(1.57?0.22)%],respectively,while CD8 [(35.37?7.55)%] was significantly higher than control group [(28.13?1.35)%],the statistical differences were significant(P

Objective To compare the difference of clinical characteristics between patients with pituitary corticotroph macroadenoma and microadenoma. Methods From 1985 to 1998, 76 inpatients with Cushing′s disease, 12 cases of macroadenoma and 64 cases of microadenoma were reviewed. The clinical manifestations, the course before diagnosis, blood routine, biochemical and endocrinic examinations were retrospectively analyzed. Results Defect of vision field and galactorrhea were more frequent and the duration of disease before diagnosis were shorter in macroadenomas than those in microadenomas. Lymphopenia, hypokalaemia, lower creatine kinase level and hyperphosphoroemia were also more pronounced in macroadenomas. In macroadenomas, the plasma ACTH and cortisol (F) concentrations at 0:00 were obviously higher than those in microadenomas, but plasma ACTH and F at 8:00 and 24h urinary free cortisol were similar in these two groups. The relative nyctohemeral variation of ACTH was less in macroadenomas than that in microadenomas, the relative nyctohemeral variation of F was similar in these two groups. One month after operation, clinicl manifestations of macroadenomas significantly improved, but the number of these patients with significant blood ACTH decrease was significantly less than that in microadenomas. Conclusion Due to higher amount and automatism of ACTH secretion, increased blood F level induces variation of clinical characteristics and laboratorial findings in patients with macroadenoma compared to the patients with microadenoma.

Objective　To investigate the relationship between the apnea events and the correspondent sleep stage. Methods 68 normal volunteers were studied with polysomnography to confirm the sleep stage of the apnea events. Results Sleep apnea events were found in each different sleep stage. The events happened frequently in light sleep stage, most in stage 2. There were no significant sleep stage distribution difference in different sex .The number and severity of apnea events were also not relative with the sleep stage distribution. Conclusion Apnea events happened most frequently in light sleep stage. The reason is still unclear.

Objective　To study the antimicrobial resistance of isolated pathogens from surgical infections in Hubei area.Methods　The diameters of the inhibition zones of surgical isolates around antibiotic susceptibility test discs in 15 hospitals were computerfiled and analysed by the software of “WHONET-4” according to NCCLS published in 1999.Results　A total of 1314 surgical isolates were collected between October 1998 and September 1999.S.aureus,P.aeruginosa,E.coli,Enterobacter sp.and CNS were the main microorganisms.44.6% of staphylococcus was resistant to oxacillin.Imipenem,amikacin,ceftazidime and ciprofloxacin were most active against Gram-negative bacilli with the sensitities (in descending order) of 84.4% to 57.2%.Conclusion　Antimicrobial resistance should be seriously considered during surgical therapy and prohylaxis with antimicrobial agents.The tendency to antimicrobial resistance of clinical isolates should be followed up continuously.

Objective To investigate professional identity and its influencing factors in nursing students during late period of clinical practice,which may be helpful for carrying out clinical nursing teaching.Methods General information and professional identity questionnaires were used for investigation in 233 nursing students of late period of intership.Results Sense of professional identity was in the general level (86.00±8.08) points.Employment situation was the main concern for students to choose nursing profession.The professional identity score of professional knowledge and skills were 15.90 and 20.88 respectively in undergraduate students,which were lower than those of college students and secondary specialized school students.Conclusions Nursing educators should pay attention to the guidance and training of professional identity in nursing students,which may contribute to the development of the nursing profession.

Objective: To determine the relationship between resilience and social support among primary school students.Methods: 2 067 primary school students from grade 3 to 5 were surveyed with questionnaires of Social Support Questionnaire and the Student Resilience Survey revised from the California Healthy Kids Survey in Hefei. Results: (1) The average scores of resilience of students in Hefei were 49.0?8.8, Girls got higher scores (50.1?8.6) than boys on resilience(47.9?8.8,t=5.71, P

Objective To investigate the feasibility of high resolution melting(HRM)curve analysis technique for detecting β-thalassemia gene mutations.Methods The HRM method was used to detect five kinds of common β-thalassemia mutations (-28, CD17,CD41-42,CD71-72 and IVS-2-654)in Guangdong province.Sixty specimens of the patients with suspectedβ-thalassemia were performed the HRM analysis.The results of HRM analysis were confirmed by the direct DNA sequencing.Results Among 60 specimens of the patients with suspectedβ-thalassemia,12 cases of heterozygosis mutant gene were detected,including 3 cases of-28,2 cases of CD17,5 cases of CD41-42,2 cases of CD71-72 and 2 cases of IVS-2-654 gene mutation;2 cases of homozygosis mu-tant gene were detected,including 1 case of-28 and 1 case of CD41-42 homozygous mutation (both were amniotic fluid specimens). The results of HRM analysis were consistent with the DNA sequencing results.Conclusion The HRM method can accurately de-tect five kinds of common β-thalassemia mutations in Guangdong province,has the advantages of simpleness,rapidness and high sensitivity and is expected to be a new method for screening β-thalassemia mutation in clinic.

At present, the particularity and importance of ethical consciousness in the teaching of clinical obstetrics and Gynecology has become increasingly prominent while the education of medical students in the ethics and social responsibility is relatively weak. This study explored a series of practical methods under WEIGHT guide through the real cases, conducting clinical training and sides debate, com-bining multimedia and interview form, in order to strengthen the cultivation of medical students' moral quality and improve the students' comprehensive ability.

Objective:To study the effect of 5-FU polylactic acid nanospheres(5-FU-PAN)on the proliferation and apoptosis of the human tongue squamous cell carcinoma Tca83 cells.Methods:Tca83 cells were exposed to 5-FU-PAN or 5-FU at the doses(mmol/L)of 1?10-2,2?10-2 and 4?10-2 for 1,3,5,7,9 and 11 days respectively.The survival rates of the cells were examined by MTT assay,the apoptosis of the cells was observed by acridine orange fluorescent staining and the cell cycle distribution was studied by flow cytometry.Results:Both 5-FU-PAN and 5-FU inhibited the proliferation and promoted the apoptosis of the cells dose and time dependantly.5-FU-PAN showed stronger effects than 5-FU.The two agents blocked the cells at S phase and 5-FU-PAN was more potential than 5-FU.Conclusions:5-FU-PAN is more effective in the growth inhibition and apoptosis promotion of Tca83 cells.