Objective To know the sanitary status of waterworks for centralized water supply in Anhui province,and try to enhance the implementation Standards for Drinking Water Quality (GB 5749-2006).Methods From April to November in 2008,the hygienic status and water quality self-test capacity of the waterworks at city,county,enterprise and town levels were investigated.Results As for the source water,over 70% of waterworks were up to the Grade Ⅲ standard.In the hygienic management and water treatment process,the city level water workswere better.The rates of complete water treatment were 80.63%,73.78%,58.12% and 60.46%,respectively for different level water works.The rates of the water quality test equipments fulfilled were 77.77%,42.99%,38.18% and 13.77% respectively.For water quality test,the 119 waterworks only focused on the aesthetic,general chemical and microbiological indexes.The performance rates of toxicological indicators,disinfectants indicators,radioactive indicators and other indicators were 17.65%,26.68%,15.97% and 3.49%,respectively.Conclusion Hygienic management of waterworks of levels for centralized water supply in Anhui province needs to be strengthened,and the water treatment process needs to be improved,the capacity building for self-test of water quality in the waterworks needs to be enhanced.

Seventy-one patients with variant angina (VA) admitted in the Cardiology Department from January 2003 to March 2011,were divided into non-stenosis group (stenosis ＜ 50％,n =43) and stenosis group (stenosis ≥50％,n =28) according to the degree of stenosis.The differences of the risk factors,clinical manifestations,electrocardiogram,echocardiogram and laboratory examinations between these two groups were compared.The average age of patients in stenosis group 58 ± 8 y was higher than that in non-stenosis group (52 ± 9 y,t =2.43,P =0.02).Other risk factors,including male gender,smoking,hypertension,diabetes mellitus and lipid disorder did not show any differences between the two groups.Percentage of patients with angina pectoris lasting less than 5 min was higher in stenosis group (x2 =5.98,P =0.02),while percentage of effort angina,seeking medical consultation ≤ 6 months of onset and hemodynamic disorders showed no difference.Laboratory examinations had no differences.It is difficult to determine whether the VA patient has fixed coronary stenosis by analyzing the risk factors,clinical manifestations and laboratory examinations; to determine the fixed coronary stenosis coronary angiography is necessary.

Objective To evaluate the perioperative safety of minimally invasive and open pancreaticoduodenectomy (PD).Methods The China National Knowledge Infrastructure,VIP,PubMed,The Cochrane Library and EMBASE were searched with the key words of laparoscopic,laparoscopy,robotic,da Vinci,minimally invasive,pancreaticoduodenectomy,微创,腹腔境,机器人,胰十二指肠切除术 to retrieve literatures on minimally invasive and open pancreaticoduodenectomy.All the patients were divided into the minimally invasive PD group and the open PD group,and the patients in the minimally invasive PD group were further divided into the robotic PD group and the laparoscopic PD group.A meta analysis was carried out using the RevMan 5.2 software.The count data were represented by the odds ratio (OR) and 95％ confidence interval (95％ CI),and the measurement data were represented by the weighted mean difference (WMD) and 95 ％ CI.Heterogeneity of the publication was analyzed using chi-square test,and the publication bias was analyzed using the funnel plots.The stability of results was analyzed using the sensitivity analysis.Results Ten literatures including 690 patients were selected.There were 235 patients in the minimally invasive PD group,and 455 in the open PD group; 107 patients were in the robotic PD group and 128 in the laparoscopic PD group.There were no significant difference in the incidence of postoperative complications,pancreatic fistula,delayed gastric emptying,postoperative hemorrhage,bile leakage,reoperation,mortality and number of lymph nodes dissected between the minimally invasive PD group and the open PD group (OR =0.75,1.01,0.98,1.50,0.94,0.56,1.06,WMD =2.29,95％ CI:0.40-1.41,0.67-1.53,0.55-1.77,0.76-2.94,0.37-2.38,0.26-1.20,0.48-2.32,-0.55-5.13,P ＞ 0.05).Although the operation time of the minimally invasive PD group was significantly longer than the open PD group,lesser volume of blood loss,shorter duration of postoperative hospital stay and higher R0 resection rate was detected in the minimally invasive PD group (WMD =99.57,-355.70,-3.30,OR =0.49,95％ CI:36.99-162.15,-608.38--103.01,-6.58-0.03,0.26-0.92,P ＜ 0.05).The operation time of the laparoscopic PD group was significantly longer than that of the open PD group (WMD =93.17,95％ CI:55.98-130.37,P ＜ 0.05).The operation time of the robotic PD group was comparable to that of the open PD group (WMD =122.96,95％ CI:-48.48-294.40,P ＞ 0.05).There was no significant difference in the duration of postoperative hospital stay between the laparoscopic PD group and the open PD group (WMD =-0.81,95％ CI:-5.08-3.45,P ＞ 0.05).The operation time of the robotic PD group was significantly shorter than that of the open PD group (WMD=-6.82,95％CI:-13.21--0.44,P＜0.05).Conclusions The perioperative safety of minimally invasive PD was comparable to open PD.Minimally invasive PD has the advantages of lesser blood loss and quick recovery of patients.Robotic PD could significantly decrease the operation time.Minimally invasive PD could be a reasonable alternative when the requirement of indications is fulfilled.

Objectives:B type ultrasonic diagnosis instrument is used to measure the sebum thickness and the relevance vs evaluated with sebum thickness calipers in sebum thickness determination. Methods:The study included 219 healthy adult persons and 11 cases of corpse dead of non disease reason for less than 10 hours.The measurements of sebum thickness were made by B type ultrasonic diagnosis instrument and sebum thickness calipers respectively. Results:The measurement results of two different methods were not significantly different. Conclusions:It is feasible to use B type ultrasonic diagnosis instrument for the measurement of the sebum thickness in clinic.

Objective To investigate the incidence of infection and the effect of anti-infection prophylaxis in renal transplanted patients after Basiliximab induction therapy. Methods A total of 204patients who have received renal transplantation and Basiliximab induction therapy from January 1,2001 to December 31, 2010 in our hospital have been retrospective analysed in this study. These patients were divided into a prophylaxis group (118 cases) with Ganciclovir + Sulfadiazine +Trimethoprim therapy and a control group (86 cases) without any anti-infection prophylaxis.Furthermore, 440 transplanted patients in the same peroid without any induction therapy were also analysed. They were also devided into two groups: an anti-infection prophylaxis group (206 cases)and a control group (234 cases) without any anti-infection prophylaxis. Results In the prophylaxis group with Basiliximab induction therapy, there were 23 patients (19. 5 %, 23/118)experienced hospitalization due to infection, 3 cases (13. 0 %,3/23) among them were severe infection, and 3patients (13.0 %, 3/23) died from vital infection. In the non-prophylaxis control group with Basiliximab induction therapy, 27 patients (31.4 %, 27/86) had infection complication, 7 patients (25.9 % ,7/27) among them were severe infection, and 4 patients(14. 8 % ,4/27)died. The incidence of infection between the above two groups is significantly different (P＜0. 05). In the prophylaxis group without induction therapy, the incidence of infection was 15.0 % (31/206), there were no severe infection cases but 7 patients (22. 6 %, 7/31) died from infection. In the non-prophylaxis control group without induction therapy, the incidence of infection was 12. 8 % (30/234), 3 cases among them were severe infection(10. 0 %,3/30)and 5 patients died from infection (16. 7 %, 5/30).The incidence of infection in Basiliximab induced patients without anti-infection prophylaxis is significantly higher than that in patients without induction therapy and anti-infection prophylaxis (31.4 % vs. 12.8 %,P＜0.01). Conclusion Basiliximab induction therapy increased the risk of infection, but not the rate of mortality. It is necessary to give anti-infection prophylaxis in renal transplanted patients with Basiliximab induction therapy.

Objective To study the treating effect of middle and high doses of acetone and aether extracts from Rumex.Japonicus Houtt on mouse thrombocytopenia.Methods 80 BACB/C mice were randomly divided into 5 groups(n=16): negative control group(salad oil),middle dose(0.5 g?kg-1) and high dose(5 g?kg-1)of acetone and aether extracts groups.All mice in various group were injected with cyclophos-phamide(0.3 mL/20 g) by hypodermic for 3 d to set up the thrombocytopenic model;after mouse thrombocytopenic model was successfully set up,the mice were given salad oil,middle and high doses of acetone and aether extracts,respectively,by gastric perfusion,20 d later the mice were executed,the numbers of the platelet,red cells and white cells were determined.Results ①The numbers of platelet numbers of all groups on the 14th day after making model decreased significantly(P0.05).Conclusion Middle and high doses of acetone and aether extracts have obviously curative effect on mouse thrombocytopenia.

Objective To study the effect of activated Notch signaling system on Schwann cells in vitro. Methods Schwann cells were isolated from sciatic nerves of adult SD rats. Recombinant rat jagged1/FC chimera, an activator of the Notch signaling system, and γ-secretase inhibitor (DAPT), an inhibitor of the Notch signaling system, were added into the culture medium respectively. The cells in the medium added with phosphate buffered saline were used as control group. Then the cultured cells were collected. NICD, as a mark of activated Notch system, was detected by immunofluorescence methods. MTT method was used to calculate the growth curve. The level of NGF in the cell supernatant was assayed by using ELISA. Results Schwann cells in recombinant rat jagged/FC chimera group grew better than those in the control groups. MTT method showed that activated Notch signaling significantly promote the proliferation of the cells(P ＜ 0.05). After cultured for 48 h, the amount of NGF in cell supernatant of recombinant rat jagged1/FC chimera group was significantly increased (P ＜ 0.05). Conclusion When the Notch signaling system is activated, Schwann cells not only proliferate but also secret more NGF.

Objective To observe the clinical efficacy of shenqifuzheng injection combined with sulfasalazine (SASP) in the treatment of ulcerative colitis (UC) ,and to evaluate the effect of CX3CR1 on colonic mucosa of UC in treatment with shenqifuzheng injection .Methods Fifty‐one patients with active mild to moderate UC were collected during the period from January 2012 to June 2014 in the second people′s hospital of Lianyungang ,which randomly divided into experimental group and control group ,and setting up health group of 15 cases .Experimental group were treated with shenqifuzheng injection combined with SASP ,control group were only with SASP ,health group were no‐treatment control .The modified Mayo scoring results and the expression of CX3CR1 on co‐lonic mucosa of the same lesion site were observed before and two weeks after treatment separately .Results The total positive rate of CX3CR1 on colonic mucosa in patients with ulcerative colitis was 88 .37% before treatment ,The total positive rate of CX3CR1 on colonic mucosa in health group was 20 .00% ,There was significant difference between two groups (Z= -2 .689 ,P<0 .01) .Two weeks after treatment ,the expression of CX3CR1 on colonic mucosa and the modified Mayo scoring results in the experimental group were significantly lower than those in the control group (P<0 .05) .The clinical comprehensive efficacy of the experimental group was significantly better than the control group (Z= -2 .085 ,P<0 .05) .Conclusion Combination of shenqifuzheng injection and SASP is more effective than using SASP alone in the treatment of UC .Shenqifuzheng injection may play important role in the treatment of UC by inhibiting the expression of CX3CR1 on colonic mucosa .

Objective:To establish a liquid chromatography tandem mass spectrometry ( LC-MS/MS) method for the determination of 5-fluorouracil (5-Fu) in patient’s plasma and apply it in clinics patients validation. Methods:5-Fu was analyzed on an Agela Inno-val NH2 (2. 1 mm × 50 mm, 5 μm) column. Methanol:ultra pure water (2 ∶98) was used as the mobile phase with isocratic elution. The flow rate was 0. 3 ml ·min-1 and the column temperature was set at 40℃. The ion transitions with electrospray ionization negative model were m/z 128. 8→42. 1 and m/z 188. 6→42. 1 for 5-Fu and 5-bromouracil (the internal standard), respectively. The LC-MS/MS method was verified according to the guideline of quantitative analysis validation of biological samples ( Chinese Pharmacopoeia, 2015 edition, the fourth part) . Results:The calibration curve of 5-Fu was linear within the range of 10-1 000 ng · ml-1 . The lower limit of quantification was 10 ng · ml-1 . The precision, accuracy, matrix effect and stability within the linear range were all in line with the requirements of method validation. Conclusion:The LC-MS/MS method developed in the study for the determination of 5-Fu is simple, rapid, accurate and reproducible, which can be used for the plasma concentration detection of 5-Fu in patients.

This study is to investigate the authenticity between COLD and HOT natural attribute in the famous Chinese medicine formulas--Zuojinwan (Coptis-Evodia 6 : 1) and Fanzuojinwan (Coptis-Evodia 1 : 6) based on mice temperature tropism, and establish an objective method to estimate the difference of two natural attribute by using a cold/hot plate differentiating technology. The results indicated that the COLD nature Zuojinwan could decrease significantly the remaining rate of HOT-symptom rat on warm pad (P < 0.05). That was not notable to COLD-symptom rat. The interference result of COLD-HOT temperature tropism to COLD/HOT symptom rat in Fanzuojinwan was the reverse with the COLD nature Zuojinwan. Meanwhile, biochemical indicators which are relative to energy metabolism such as ATPase enzyme activity and total anti-oxidant capability (T-AOC), had corresponding change in the organism. In the study, the COLD and HOT natural tendency in Zuojinwan and Fanzuojinwan which were composed by the same herbs with different proportion could be expressed qualitatively, quantitatively, objectively and directly with applying animal temperature tropism, and be verified to philosophical idea of treating disease theory with "expelling heat with cold herbs and cryopathy requiring warm prescription", not "expelling heat with heat herbs and cryopathy requiring cold prescription" in ancient traditional Chinese medicine, which brings a new approach in investigation of the nature theory of traditional Chinese medicine.