1.Development of internet-based home tele-monitoring system for fetus
Chinese Medical Equipment Journal 1989;0(02):-
The internet -based home tele -monitoring system for fetus has the functions of monitoring fetal heart and movement, analysis and alarm. With this system, data related to fetuses can be shared and real -time analyses and suggestions from doctors can be available. Current network system is involved in, and thus it's inexpensive and practical.
2.Determination of Residues of Organochlorinated Pesticides in Polygonum multiflorum by GC-MS with Solid-phase Extraction
China Pharmacy 2005;0(19):-
OBJECTIVE:To determine the residues of 9 kinds of organochlorinated pesticides in Polygonum multiflorum.ME-THODS:Samples were extracted with ethyl acetate by ultrasonic wave assistant extraction and purified on Florisil solid-phase extraction column.The residues of organochlorinated pesticides were determined with capillary gas chromatography with GC-MS.The separation was performed on DB-5MS(30 m?0.25 mm,0.25 ?m) fused-silica capillary column with injector temperature of 230 ℃.The initial column temperature was 100 ℃,then raised to 220 ℃ at the rate of 8 ℃?min-1.The column temperature was ra-ised at 250 ℃ at the rate of 5 ℃?min-1 for 10 min.The column flow was 1 mL?min-1 and injection volume was 1 ?L.RESULTS:9 kinds of organochlorinated pesticides were completely separated within 30 minutes.The average recoveries ranged from 80.4% to 97.2%(RSD ranged from 3.5% to 7.4%,n=6).CONCLUSION:The method is sensitive and accurate for the content determination of the residues organochlorinated pesticides.
3.Progress on stent graft induced new entry after Thoracic endovascular aortic repair
Hongbo CI ; Qingbo FANG ; Xiaohu GE
International Journal of Surgery 2015;42(12):838-841
Thoracic endovascular aortic repair (TEVAR) has been increasingly used in the treatment of Stanford type B dissection.The incidence of new entry after thoracic endovascular aortic repair has been gradually increased report including at the proximal end and at the distal end of the endograft.New entry is difficult to handle following thoracic endovascular aortic repair for aortic dissection,and associates with a high substantial mortality.It need pay more attention to prevention and treatment on new entry after thoracic endovascular aortic repair.We summary and analyze the possible causes,prevention and management of new entry after thoracic endovascular aortic repair for aortic dissection.This article review and conclusion the progress on stent graft induced new entry after thoracic endovascular aortic repair.
4.The effect of growth hormone in poor responders undergoing COH
Fang NIU ; Liuming LI ; Hongbo WU
Chinese Journal of Primary Medicine and Pharmacy 2014;(19):2902-2904
Objective To observe the efficacy of growth hormone ( GH) co-treatment in poor responders undergoing IVF-ET.Methods To analyze all the ovaries low response patients treated by IVF/ICSI,80 cases were ran-domly divided into two groups:40 patients in GH group were given GH along with Gn daily until the HCG administra-tion.40 patients in the control group received the same treatment protocol except the GH co -treatment.The catching spawn number and MⅡova,fertile rate,the number of perfect embryos ,pregnant rate were observed .The serum con-centrations of IGF-I,IGFBP-3 were detected by ELISA and compared between the two groups .Results The number of oocytes retrieved ,number of MII oocytes ,cleavage ,number of embryos available of GH group were increased without statistical significance(all P>0.05 ).The serum IGFBP-3 level of 2 groups had no statistical significance .The fertile rate,pregnant rate ,serum IGF-I level were significantly higher in GH group than those in the control group ( all P<0.05).Conclusion GH co-treatment in poor responders could improve the outcome of IVF-ET,which imply that GH could improve the quality of oocytes and embryos related to the elevated IGF-1 level in serum .
5.Integrative treatment of multiple trauma patients with severe craniocerebral injury in emergency center (with 148 cases report)
Guangyu WU ; Hongbo XU ; Feng ZHAO ; Lijun TAO ; Jun FANG
Chinese Journal of Postgraduates of Medicine 2006;0(35):-
Objective To explore the significance and important measure of integrative treatment of multiple trauma patients with severe craniocerebral injury in emergency center. Methods One hundred and forty-eight multiple trauma patients with severe craniocerebral injury in emergency center from November 2002 to February 2006 were analysed retrospectively. Result In total 148 cases, 72 were cured and 26 dead, 7 were in status of plant man, 17 experienced severe deformity, and 26 did mild deformity. Conclusion Multiple trauma patients with severe craniocerebral injury usually experience a serious situation and rapid development. Therefore emergency doctors are required for organizing salvage of such patients, at the same time treatment and diagnosis are implemented. Firstly the most important key to successful salvage is appropriate disposal of fatal injury and early elimination of shock. Proper surgical choice, especially at the first time, inspection and protection of visceral functions, and attention to nutritional support are other vital methods to gain more successful salvage. ICU is also emphasized for its essentiality.
6.To explore the distribution characteristics and clinical typing methods in distal crevasses of Stanford B aortic dissection
Hao REN ; Hongbo CI ; Qingbo FANG ; Sheng GUAN ; Xiaohu GE
International Journal of Surgery 2014;41(12):824-826,封3
Objective To explore the distribution characteristics and clinical typing methods in distal crevasses of Stanford B aortic dissection.Methods Review of the cases in the People's Hospital of Xinjiang Urgur Autonomous Region from 2010 January to 2013 June were diagnosis of Stanford type B aortic dissection with computed tomographic angiography data,Observed its distal crevasses distribution and statistical its number,then summarizes the distribution characteristics of the distal crevasses and further put forward a method of clinical typing.Results Refer to 115 cases with Stanford type B aortic dissection computed tomographic angiography data,including 101 cases with distal crevasses (87.83%) and a total of 240 distal crevasses,an average of 2.37 per case.Conclusions The distal crevasses more often appear in the area involving visceral artery,combined with its different in distribution characteristics and processing methods,we put forward the classification method,namely:Type Ⅰ:the distal crevasses are located in the zone of the thoracic artery; Type Ⅱ:the distal crevasses are close to the visceral artery or involvement it; Type Ⅲ:the distal crevasses are lower than the renal artery,not involving the visceral artery; Type Ⅳ:the distal crevasses are located in the zone of the iliac artery.
7.Catheter-directed thrombolysis versus anticoagulant alone for treatment of deep venous thrombosis: a Meta-analysis of randomized trials
Sheng GUAN ; Qingbo FANG ; Hongbo CI ; Xiaohu GE
International Journal of Surgery 2015;42(12):803-807
Objective To evaluate the efficacy and safety of catheter-directed thrombolysis (CDT) combined with anticoagulant compared with traditional treatment (Anticoagulant alone A C) for deep venous thrombosis.Methods We searched Medline,Embase,Cochrane Central Register of Controlled Trials,PubMed,Chinese Biomedical Literature Data Base (CBM),Chinese Scientific Journal,Full-text Data Base (CSJD),and,added with hand searcing and other retrievals.The Cochrane Collaboration's RevMan 5.0.18 was used for Meta-analysis.Results Four randomized controlled trials were available and were included in the study.Meta-analysis showed that 6 months after treatment,iliofemoral vein patency rate of CDT group was higher than that of AC group (OR =5.13,95% CI:2.01-13.14,P =0.0006);Major complications of CDT group compared to those of AC group were not statistically significant(OR =2.74,95% CI:0.76-8.07,P =0.13),but the minor complications and total complications of CDT group were higher than those of AC group [(OR =7.86,95% CI:3.10-19.90,P <0.0001)and(OR=5.42,95%CI:1.47-20.01,P=0.01)].Conclusions CDT is a positively effective way to treat early DVT.Application of CDT in patients without contraindications to its use can have good therapeuic effect.
8.Preliminary clinical study on distal-end tear of Stanford type B aortic dissection
Yufeng XIAO ; Qingbo FANG ; Bing ZHU ; Hongbo CI ; Xiaohu GE
International Journal of Surgery 2016;43(3):178-181
Objective The objective of this article is to attempt to propose the endovascular repair principles of distal-end tear of Stanford type B aortic dissection.Methods The vascular surgery of xinjiang uygur autonomous region people's hospital received and cured 101 patients of Stanford B aortic dissection from January 2013 to January 2015.The patients are divided into two groups according different treatment principles:(1)There are 57 cases in sequential treatment group,performing endovascular repair of aortic tears from near to far,(if the tear at visceral artery is not treated then the distal-end tear is also not treated);(2) There are 44 cases in non-sequential treatment group,not performing endovascular repair of aortic tears from near to far (the tears involving visceral artery are not treated and the remaining distal-end tears are performed endovascular repair).After operation,carry out statistical analysis between two groups on the growth rate of aortic diameter of the coeliac axis,occurrence rate of main discomfort complaint,false lumen thrombosis rates.Results After operation,between the two groups,the growth rate of aortic diameter of the coeliac axis is obvious difference(P < 0.05),that the sequential group is with a low rate;there are obvious differences on the occurrence rates of main discomfort complaint and false lumen thrombosis rates (P < 0.05),that the sequential group is superior to the non-sequential group.Conclusions After a preliminary clinical study,we get a conclusion that when treating distal-end tears of Stanford type B aortic dissection,sequential treatment is better than non-sequential treatment.
9.Twelve cases report of the vagus splenic aneurysm and literature review
Qingbo FANG ; Hongbo CI ; Yufeng XIAO ; Sheng GUAN ; Xiaohu GE
International Journal of Surgery 2015;42(3):180-182
Objective To assess the treatment of splenic artery aneurysms(SAA) and curative effect evaluation.Methods Twelve SAA patients treated in our hospital from January 2012 to May 2014 were clinical analyzed.The male in Twelve patients was 4 man and others were female.The vagus splenic artery aneurysms are originated from the superior mesenteric artery,tumors are single,from 1.5cm to 2.8cm in diameter,an average of 2.1cm.Twelve cases were performed surgery,4 patients underwent elective surgery,interventional embolization of the splenic aneurysm in 3 patient,The others were performed interventional embolization + superior mesenteric artery covered stents.Results Technical success was achieved in all twelve patients,2 patients had adverse effects such as abdominal pain,fever,etc.There revealed no aneurysm recurrence was found.Twelve patients were followed for 6-24 months,the follow-up by examinations with electronic computer X-ray tomography or color Doppler ultrasonic as well as angiography every 3 months.One patient died of severe abdominal bleeding 1 year later after the operation and the other eleven patients remained in good condition with no occurrence of re-canalization of the lesions.Conclusions For the vagus splenic aneurysm with suitable for anatornic conditions,cavity therapy is safe and effective,for the vagus splenic aneurysm involving hepatic artery,need to open surgery for vascular remodeling.
10.Directional differentiation of bone marrow mesenchymal stem cells into oligodendrocytes induced by the combination of various neurotrophic factors
Yongtao XU ; Feng LI ; Tie LIU ; Hongbo YOU ; Zhong FANG
Chinese Journal of Tissue Engineering Research 2009;13(14):2793-2796
BACKGROUND: Myelination following axonal regeneration is a key factor affecting the recovery of spinal cord injury. Oligodendrocyte survival directly affects the myelination following axonal regeneration. OBJECTIVE: To investigate the feasibility of differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into oligodendrocytes induced by neurotrophic factors. DESIGN, TIME AND SETTING: The cell molecular biology in vitro study was performed at the Laboratory of Department of Orthopaedics, Tongji Hospital from September 2006 to June 2007. MATERIALS: A total of 5 Sprague Dawley rats aged 2-4 weeks, of both gender were selected. Bilateral femur and tibia bone marrow was obtained to harvest BMSCs. METHODS: At passage 4, BMSCs were incubated in serum-free medium, supplemented with N2, 20 ng/mL basic fibroblast growth factor, 20 ng/mL epidermal growth factor for 48 hours, and incubated in medium containing 500 ng/mL insulin-like growth factor I and N2 for 3 days. MAIN OUTCOME MEASURES: Morphological changes were observed using an phase contrast microscope. Semiquantitative RT-PCR was utilized to detect specific marker mRNA expression of oligodendrocytes. Using neuron marker anti-microtubule-associated protein, astrocyte marker anti-glial fibrillary acidic protein, oligodendrocyte marker anti-galactocerebroside, anti-myelin basic protein antibody, immunocytochemical staining was performed to detect the positive rate of the differentiation of BMSCs into oligodendrocytes. RESULTS: Morphological changes in BMSCs during the differentiation into oligodendrocytes: After the induction, a majority of BMSCs presented the morphological characteristics of oligodendrocytes. Cytoplasm retraction towards nucleus, cell process extension towards outwards, and strong refraction were found. With the prolongation of time, several cell processes connected and formed a typical net-shape structure. Specific marker mRNA expression of oligodendrocytes: Following induction, specific strap of myelin basic protein mRNA and galactocerebroside mRNA could be detected. Positive rate of oligodendrocytes: During induction, the positive rates of galactocarebroside, myelin basic protein and microtubule-associated protein were 65%, 45% and 10%, respectively. CONCLUSION: The combination of epidermal growth factor, basic fibroblast growth factor and insulin-like growth factor can effectively promote the directional differentiation of BMSCs into oligodendrocytes.