Cellular senescence is a permanent cell cycle arrest that may prevent the aged or abnormal cells from further expansion and is therefore a safeguard mechanism against unlimited cell proliferation.Recent studies demonstrated that oncogene-induced senescence could prevent neoplastic transformation in vitro and in vivo,and oncogene might cause tumors only in senescence deficient cells.Although the mechanism of the cellular senescence has not been completely illustrated,activation of P16INK4a-RB and ARF-P53 pathways has been implicated in this process.Further investigation of the signaling pathways leading to senescence may offer new avenues for tumor treatment.

Rapamycin is frequently used as an immunosuppressive agent and also plays an important role in tumor suppression.It combines to FKBP12 and forms a compound to inhibit the serine/threonine protein kinase mammalian target of rapamycin(mTOR),its activation is required for protein synthesis and cell-cycle progression.Rapamycin can block cell cycle at G1 phase and to inhibit cell proliferation.Furthermore rapamycin may inhibit cytokine production and cytokine signaling.Therefore rapamycin is used in the treatment of post-transplantation organ rejection and tumor therapy due to its dual effects of immuno-suppression and tumor inhibition.

Objective:To explore the relationship of Syk and VEGF-C expression in breast cancer with lymph node metastasis . Methods:The expression of Syk and VEGF-C in 40 cases of breast cancer were determined using immunohistochemistry assay .Then, the positive rates of Syk and VEGF-C were compared with lymph node metastasis .Results:The expression rate of VEGF-C and Syk in breast cancer was respectively 77.5%(31/40), 37.5%(15/40).VEGF-C protein expression was not significant related with the tumor diameter (P>0.05) and histological grade (P>0.05), but was significantly associated with lymph node metastasis (P<0.05).Syk protein expression was not significant related with tumor diameter (P>0.05) and the histological grade(P>0.05), and was associated with lymph node metastasis (P<0.05).The difference of Syk and VEGF-C had statistics significance(P<0.05).Con-clusion:Syk and VEGF-C involved in the lymphatic invasion and metastasis , may be an important indicator of prognosis .

Objective:To analyze the impacts of sustainable deterioration of environment quality and demographic structure adjustment on urban residents' health expenditure.Methods:Non-spatial panel model and spatial panel model were applied to use inter-provincial panel data to conduct the empirical analysis.Results:Environment quality,demographic age structure and interaction had significant impact on urban residents' health expenditure.Conclusion:Demographic structure transformation and environment quality deterioration were the two difficulties faced by China's medical and health system.The construction and reform of medical and health system should focus on these challenges.

Objective:To explore the establishment of medical equipment safety risk management system new pathways.Methods: By studying the Medical Device Clinical use of safety management practices (Trial) and the three general hospital accreditation standards Implementing Rules (2011 Edition) Chapter VI (hospital management) Article 9 (Medical Equipment Management) accreditation standards.Results: Risk control made in accordance with relevant national policies and related evaluation criteria four off management model, and to establish an effective risk management system of medical equipment safety.Conclusion: The establishment of medical equipment safety risk management system helped to ensure medical quality and safety, to provide reference for the clinical use of medical equipment to explore the system security risk management through.

Objective To explore the effect of estrogen on the permeability of retinal blood vessel by ovariectomy. Methods Twenty-two healthy rats were divided into experimental and control group randomly. Estrogen level of rats decreased due to ovariectomy in the experimental group while stabilized by sham-ovariectomy in the control group. The results were confirmed by vaginal epithelium smearing. Retinal vein occlusion was established by photodynamic method, and leakage of Evan's blue in retina was determined by spectrophotometer. Results Mature value of vaginal epithelium decreased significantly in ovariectomy rats( t=21.008,P =0.000) while not significantly in sham-ovariectomy ones ( t=0.319,P =0.756); the mean leakage of Evans blue was (25.503 0?4.378 47) ng/mg in experimental group, and (17.830 0?4.265 69) ng/mg in the control group, and the difference between the two groups is significant( t=3.969 36,P =0.001). Conclusion Ovariectomy is an useful method to study the effect of estrogen on ocular diseases, and when estrogen level decreases, the permeability of retinal blood vessel increases.

Objective To compare the clinical value of two kinds of imunoassay method including CLIA and ELISA in the detection of related antibody of patients with HCV.Methods 60 patients with HCV were chosen as study subjects,and the related antibodies of patients were detected by CLIA and ELISA.The positive rates of anti HCV antibody,anti-3E antibody,anti-GP210 antibody,anti-SP100 antibody,anti-PML antibody and anti-AMA -M2 antibody detected by CLIA and ELISA were compared.Results The positive rates of anti-HCV antibody detected by CLIA and ELISA were 83.33％ (50/60),98.33％ (59/60),respectively.The anti-HCV antibody detected by CLIA was significantly higher than by ELISA (x2 =4.73,P =0.03).The positive rates of anti-3E antibody,anti-GP210 antibody,anti-SP100 antibody,anti-PML antibody and anti-AMA-M2 antibody detected by ELISA were 11.67 ％ (7/60),6.67 ％ (4/60),11.67 ％ (7/60),10.00 ％ (6/60),11.67 ％ (7/60),respectively,those detected by CLIA were 73.33 ％ (44/60),50.00％ (30/60),38.33 ％ (23/60),58.33 ％ (35/60),80.00％(48/60),respectively.The positive rates of anti-3E antibody,anti-GP210 antibody,anti-SP1O0 antibody,anti PML antibody and anti-AMA-M2 antibody detected by CLIA were significantly higher than by ELISA(x2 =18.81,11.51,13.23,15.08,13.75,all P＜0.05).Concelusion Compared with ELISA,CLIA in the detection of related antibodies of patients with HCV can efficiently higher the positive rate of anti-HCV and autoantibody.

Objective:To examine the sodium-hydrogen exchanger(NHE-1) m RNA expression in lung of patients with congenital heart disease,and to explain the cause of pulmonary vascular remodeling.Methods:Twenty-two patients with congenital heart disease were divided into 2 groups:non-pulmonary-hypertension group(s PAP

Objective To study the curative effect of combing montelukast with inhaled corticosteroids in the treatment of cough variant asthma .Methods According to diagnosis standard guideline for diagnosis and treatment of cough in CVA,84 cases of CVA patients were chosen and divided into the treatment group (n=42)and the control group(n=42).The control group were treated with inhaled corticosteroid Budesonide .The treatment group was given monelukast based on the treatment in control group .And the clinical effects of two groups ,recurrence and the lung function index were observed .Results The total efficiency of treatment of the control group was 73.8%,and the treatment group was 95.0%.The total effective rate of observation group was apparently higher than that of the control group(χ2 =6.46,P<0.05).The lung function of the treatment group compared to the control group was significantly increased(t=2.873,P<0.05).Conclusion Montelukast combined with Glucocorticoid in the treatment of CVA can effectively improve the cure rate ,reduce the recurrence ,which can be widely used in clinical treatment .

urpose To investigate apoptosis in vitrectomy specimens of proliferative vitreoretinopathy. Methods Vitrectomy specimens from 60 cases of different classes of proliferative vitreoretinopathy were studied by TdTmediated dUTP nick end labelling(TUNEL)method. Results The characteristic change of apoptosis was observed in all vitrectomy specimens.The amount of apoptotic nonpigmentary cell is gradually decreasing along with the development of proliferative vitreoretinopathy,and apoptotic pigmentary cells are observed. Conclusion There are different kinds of apoptosis cell in vitrectomy specimens of proliferative vitreoretinopathy.It is suggested that apoptosis might be one of the important mechanisms of regulating the degree in proliferative vitreoretinopathy.