To explore the therapeutic effects and toxic side-effect of 131I-McAb (S102, T9, CL-3) in the treatment of hepatocellular carcinoma (HCC). 131I-McAb, which was labelled by IODO-GEN method, with 50-70mCi of 131I, was percutaneously injected into the tumor under B-ultrasonic guidance in 116 patients. The results showed that the effective rate (CR+PR) was 29.3% (34/116), the overall effective rate (CR+PR+MR) was 53.4% (62/116), the one-year survival rate was 38.2% (39/102), the mean survival time was 34.7 weeks, and the mean tumor developing phase was 12 weeks. The results suggested that the use of 131I-McAb in the treatment of HCC is a low toxic, effective and minimal invasive operation.

Based on a study of 33 cases of medical tangle wherein demand for compensation for mental damage was met, the paper analyzes the current status of demand for compensation for mental damage, including the cause, extent and smount of money. It also discusses the double effect of mental compensation and points out that perfecting the establishment of medical laws and regulations is the only way which must be passed in solving issues of medica1 tangle concerning mental compensation.

OBJECTIVE To evaluate the functional outcomes in patients undergoing supracricoid partial laryngectomy(SCPL).METHODS Twenty-one cases underwent supracricoid partial laryngectomy with functional reconstruction.In these cases.14 were glottic cancers(cricohyoidoepiglottopexy,CHEP),7 were supraglottic cancers(Cricohyokdopexy,CHP). RESULTS The mean time of decanulation was36.4 days,decannulation rate was 90.5%(19/21).All the patients recovered the function of speech and swallowing,but had variously hoarse.CONCLUSION The SCPL technique is effect in laryngeal functional reservation of laryngeal neoplasms.

Objective To develop a gas chromatography-mass spectrometry (GC-MS) detection method for quantification of the nonpolar amino acids in amniotic fluid of congenital malformation including alanine (Ala),leu cine (Leu),isoleucine (Ile),proline (Pro) and phenylalanine (Phe).To compare the different amino acids in amniotic fluid between pregnant women with congenital malformation and normal control and analyze the corresponding pathogenesis.Methods After precipitated protein of the 100 μL supernatant of amniotic fluid by methyl alcohol,the supernatant was dried by nitrogen.The extractions were treated with MSTFA for derivatization.Then gas chromatography-mass spectrometry was used to detect and analyze the amino acids.Results This method was proved to have good sensitivity,precision,accuracy and recoveries.Under the optimum testing conditions,the limit of detection (LOD) was 0.12-0.38 mg/L.The calibration curves showed good linearity over the investigated concentration range between 0.5 and 10 mg/L.The recoveries were 91.12％ to 104.41％.The relative standard deviation of intra and inter-day was 0.84％ to 9.33％.The developed method was applied to the quantification of 5 nonpolar amino acids in amniotic fluid of 17 pregnant women with congenital malformation and 13 normal control pregnant woman.The contents of leucine and isoleucine decreased in disease group compared to controls.The difference of the other three amino acids between the two groups had no statistical significance.Conclusions The validation results showed that the method was suitable for detection of the amino acids in amniotic fluid and having broad prospect of clinical application.Leucine may participate in the pathogenesis of congenital malformation.

Objective To study the genetic relationship of the Y chromosomal short tandem repeat gene loci in Lanzhou Han population and other 25 populations .Methods The frequency of alleles of Y-STRloci was obtained from a sample of 500 unrelated individuals living in Lanzhou City , and other 25 populations in different areas collected from the published data were used to calculate the genetic similarity coefficient and genetic distance .Phylogenetic trees based on the genetic distance were established .Results Populations of Lanzhou , Beijing, Shanxi and Inner Mongolia were in an identical cluster .Compared with minorities , the genetic distance between Lanzhou Han population and Inner Mongolia Mongolian population was dramatically smaller from other subpopulations .The populations in Malays and Indians were far from the other groups .Conclusion The Y-STR gene frequency distribution in 26 populations has identified differentiation in race, clime and evolution, and it is basically identical with the classification of human races which is similar to or according with other molecular anthropology research conclusions .

Objective To com pare the concentration of teeth DNA extracted by three different pretreatm ent m ethods and to explore a sim ple, econom ical and practical pretreatm ent m ethod w ith high concentration of extracted DNA from teeth. Methods A total num ber of 21 m olars w ere collected from 7 corpses. The pretreatm ent of 3 m olars from each individual w as random ly perform ed by tooth crum b m ethod, ball-m illing m ethod and liquid nitrogen m illing m ethod and 50 m g tooth crum b w as w eight and DNA w as extracted by A utoM ate ExpressTM forensic DNA extraction system . Subsequently, the concentration of DNA and corresponding STR genotyping of three m ethods w ere com pared. Results The DNA concentration extracted by tooth crum b m ethod, ball-m illing m ethod and liquid nitrogen m illing m ethod w as 0.055 6-1.989 1 ng/μL , 0.036 6-1.175 6 ng/μL and 0.037 8-1.249 0 ng/μL , respectively. The DNA concentration ob-tained by tooth crum b m ethod w as higher (P<0.05) and the success rate of STR genotyping w as high. Conclusion C om bined w ith A utoM ate ExpressTM forensic DNA extraction system , tooth crum b m ethod is an efficient and feasible m ethod to extract DNA from teeth, w hich can be applied in forensic practice.

Objective To determine the genetic polymorphism of 24 Y-STR loci haplotype and investigate its application value in legal physical evidence.Methods AGCU Y24 kit and 3130xl Genetic Analyzer were used to detect the distribution of 24 Y-STR loci including DYS391,DYS389Ⅰ,DYS439,DYS389Ⅱ, DYS438, DYS643, DYS456, DYS458, DYS437, DYS635, DYS448, DYS527a/b, Y-GATA-H4, DYS447, DYS19,DYS392,DYS522,DYS393,DYS388,DYS390,DYS385a/b and DYS444in 154 unrelated individuals of Dongxiang ethnic minority males in Gansu province of China.Results A total number of 153 haplo-types were detected in 154 samples, the haplotype diversity was 0.9915 and the discrimination power was 0.9940.Conclusion The 24 Y-STR loci system has high haplotype diversity and discrimination power.

The keratocystic odontogenic tumor (KCOT) is a common cystic lesions of jaw, which has a high recurrence rate but rarely undergo malignant transformation. This study reported a patient with primary intraosseous squamous cell carcinoma developed from KCOT.
Carcinoma, Squamous Cell ; etiology ; Cell Transformation, Neoplastic ; Humans ; Odontogenic Tumors ; complications

Background and purpose:Surgical operation is the main method in the treatment of laryngeal carcinoma, but different patients have different impacts on the survival time and the quality of life with different type of operation. This study was to analyze the methods of surgical treatment and its long-term effect of laryngeal carcinoma, to increase survival rates, laryngeal function reservation and reconstruction and improve life quality.Methods:A total mumber of 424 patients of laryngeal carcinoma treated with surgical treatment during between Jan. 2002 and Dec. 2012 were researched by clinical follow-up and data analysis. Surgical method: CO2 laser-assisted laryngeal microsurgery for laryngeal tumors for 50 cases, frontal partial laryngectomy or modiifed thyroid cartilage window partial laryngectomy without tracheostomy for 42 cases, vertical frontolateral partial laryngectomy for 119 cases, horizontal partial laryngectomy and extended subtotal laryngectomy for 22cases, anastomosis of pharynx and trachea for 4 cases, supra cricoid partial laryngectomy for 129 cases (CHEP of them for 103 cases, CHP for 26 cases), total laryngectomy for 58 cases, cervical lymph node dissection at the same term for 121 cases.Results:Partial laryngectomy without tracheostomy for 92 cases (21.7%), total laryngectomy for 58 cases (13.7%); decannulation rate was 86.5%, vertical frontolateral partial laryngectomy of them for 93.2%, horizontal partial laryngectomy of them for 90.9%,supra cricoid partial laryngeal of them for 82.2%; laryngeal function reservation rate for 86.3%; all patients did outpatient review and telephone follow-up, 9 cases loss to follow-up, a total of tumor recurrence, cervical lymph node metastasis and distant metastasis were 41 cases, mostly occurred in 1 year after surgery; death for 57 cases, relapse of them for 8 cases, cervical metastasis for 13 cases, pulmonary metastasis for 5 cases, hepatic metastases for 2 cases, brain metastases for 1 case, esophagus metastases for 1 case, pulmonary infection for 6 cases, acute renal failure for 2 cases, unknown reason for 19 cases, according to Kaplan-Meier to count survival rate, 3-year and 5-year survival rate were 90.7% and 84.1%, relapse and metastasis were the main causes of death.Conclusion:Surgical treatment is the main therapy mode of laryngeal carcinoma. We choose individualized surgical methods for patients according to tumor staging, invasion site, age, occupation and education background of patient, health condition and so on. On the premise that tumor is completely cut off, we always advocate function surgery and minimally invasive surgery, and adopt comprehensive treatment at the same time, in order to increase survival rates, lesson suffering and improve life quality as far as possible.

OBJECTIVE To study the distribution and drug resistance of meticillin-resistante coagulase negative Staphylococcus(MRCNS) in neonate septicemia for rational use of antibiotics in clinic. METHODS The MRCNS isolates were detected by BD120 and ATB.Susceptibility test to 12 antibiotics was performed through disk diffusion test. RESULTS Totally 262 strains coagulase negative Staphylococcus(CNS) was isolated from 344 S.aureus strains.Among 262 CNS,there were 130 S.epidermidis strains(49.6%),94 S.hemolyticus strains(35.9%),21 S.hominis(8.01%),and 17 other kinds of Staphyloccus strains(6.49%).The isolated ratio of MRCNS was 65.0%(170/262).Susceptibility test showed that the MRCNS were multiple resistant. CONCLUSIONS CNS occupys the first place in infection of neonatal blood.The isolated ratio of MRCNS is high and shows multi-drug resistance.Vancomycin and some other glycopeptide antibiotics are the first choice for MRCNS infections.