Objective In acidity condition, arsenic and mercury can react on newly produced H2 to produce hydride which can be detected by atomic fluorescence spectrometry. The goal of the present paper is to establish a method to determine arsenic and mercury of the water at one time. Methods A quantitative method for determination by hydride generation-atomic fluorescence spectrometry was used .The electric current of the arsenic and mercury lamp were all 80 mA. The ratio of main arsenic electric current with the assist one was 50:30. 1% KBH4 of 0.2% NaOH solution were used as the reducers. Results The linear range of the arsenic was 0-10 ?g/L with correlation coefficient r=0.999 9. The RSDs and recovery rates of the arsenic were 2.0%-5.3% and 84%-113% respectively. The mercury showed a good linear result in the range of 0-5 ?g/L either, and the correlation coefficient was 0.999 3. The RSDs and recovery rates of the method were 1.9%-5.7% and 84%-107% respectively. Conclusion This method is simple, rapid, sensitive and accurate. It can determine arsenic and mercury in water at one time.

BACKGROUND:Hematopoietic stem cel transplantation is a promising treatment for a variety of malignant and nonmalignant disorders. But noninfectious pulmonary complications folowing hematopoietic stem cel transplantation are a major cause of morbidity and mortality in these patients. And the diagnosis and treatment are difficult. OBJECTIVE: To review the noninfectious pulmonary complications after hematopoietic stem cel transplantation in terms of onset time, risk factors, clinical manifestations, characteristics of the high-resolution CT, histopathological measurement, related genes and treatment options. METHODS: A computer-based search of VIP, PubMed and Sciencedirect databases was performed for articles related to noninfectious pulmonary compli cations after hematopoietic stem cel transplantation published from January 2005 to October 2014. The key words were “HSCT, pulmonary complications, non-infectious, pirfenidone” in Chinese and English in the title and abstract. Finaly, 31 articles were included in result analysis. RESULTS AND CONCLUSION: The incidence of noninfectious pulmonary complications after hematopoietic stem cel transplantation has become more and more, with atypical clinical manifestations and limited diagnosis and treatments. According to the different clinical onset time, diagnostic criteria, clinical manifestations and the appropriate laboratory tests, clinicians can make early diagnosis and early intervention. Especialy, the usage of high-resolution chest CT and bronchofiberscope for bronchoalveolar lavage as wel as the timely drug administration can improve the survival rate of patients with noninfectious pulmonary complications after hematopoietic stem cel transplantation.

OBJECTIVE:To study the effect of kallidinogenase on senile diabetic nephropathy.METHODS:95senile cases with diabetic nephropathy treated with the basic therapy were randomly divided into treatment group(plus kallidino?genase)and control group(plus aspirin),the proteinuria ejection level,hemorheological parameters and blood lipid level of2groups in24hours were investigated before and after treatment.RESULTS:As compared with prior treatment and the coutrol group,the proteinuria ejection level decreased significantly,the hemorheology improved and the blood lipid level decreased in the treatment group.CONCLUSION:Kallidinogenase is effective to some degree in the prevention and cure of senile diabetic nephropathy and diabetic macroangiopathy.

Objective To establish the analytical methods for determining the contents of tannins in different vegetative organs of Abutilon indicum (L.) Sweet. Methods The content of tannins, expressed as gallic acid equivalents, was measured by using the phospho molybdenum tungstic acid-casein colorimetry. Results The good linearity between concentrations and absorbances of gallic acid was found in the range of 2～10 mg/L, the average recovery was 99.63% and RSD was 1.08%, the contents of tannins in leaves, stems and roots of Abutilon indicum (L.) Sweet were 0.34%, 0.16% and 0.27% respectively. Conclusion The proposed method is sensitive, precise, accurate and reproducible, and it is suitable for the determination of tannins in Abutilon indicum (L.) Sweet.

Objective To test the strength of different suture fixation of tendon or ligament end and evaluate appropriate stitches number in cruciate ligament reconstruction. Methods Twenty-four specimens of patellar tendon with free ends, which was, divided into 3 groups according 2 or 3 stitches and 6 subgroups using different suture materials, either with No1 Ethilon or 0.4 mm diameter stainless steel wire, fixed with Krackow locking stitch, the tensile test were undertaken respectively, so as to find out the least stitches and efficient suture pattern. Results Two Krackow locking stitches had more strength than that of 0.4 mm diameter stainless steel wire itself, the fixation strength of 2 stitches which used No1 Ethilon was more than 80 N, superior to the failure strength of material itself, it still maintained the same strength if the first stitch was across the tendon tissue transversely. On the basis of testing results, this modified stitches pattern was used in 17 cases, all could be well withstand an accelerated postoperative ROM rehabilitation program in clinical practice. Conclusion There is no significant difference between 2 and 3 Krackow locking stitches in suture strength statistically, the fixation strength is superior to the suture material, it can lessen the suture exposure when the first stitch is across the tendon tissue while maintains a comparable strength to other suture. If attempt to make a higher strength of suture, one should focus on selecting a stronger materials or adding the suture materials in number, therefore, a rapid early ROM rehabilitation is possible and reliable in practice.

Objective To investigate a water contamination accident caused by industrial wastewater during Feb 27th-29th ,2004,in Tuojiang river, analyze the variation of sanitary quality of drinking water during and after the accident. Methods After the occurrence of the accident,consecutive surveillance and analyses were done to discover the variation of the levels of amino-nitrogen,nitrite and nitrate in source water and finished water of Jianyang Water Works and Neijiang No 2 Water Works,which located at the lower reaches of Jianyang. Results The levels of amino-nitrogen in source water and finished water of Jianyang Water Works reached the first peak [(47.04?2.48)?(45.97?2.37)mg/L]at the 3rd day(March 3rd) after the end of discharge of industrial wastewater from contamination source,decreased at the 4th day ,reached the second peak at the 5th day[(42.26?2.88)?(39.50?2.64)mg/L], then decreased gradually, presented the normal levels at the 11th day[(0.54?0.10)?(0.30?0.12)mg/L]. The levels of nitrite in source water and finished water of Jianyang Water Works reached the first peak [(2.09?0.23)?(0.91?0.15)mg/L] at the 2 nd-3rd day after the end of discharge of industrial wastewater from contamination source,decreased at the 4th day ,reached the second peak at the 5th day[(2.18?0.33),(0.61?0.16)mg/L], then decreased gradually,presented the normal levels at the 11th day[(0.16?0.07)?(0.02?0.01)mg/L]. The levels of nitrate in source water and finished water of Jianyang Water Works(3.56～9.15 mg/L) didn't exceed the standard ruled the Sanitary Standard for Drinking Water (2001) throughout the duration of accident. The levels of amino-nitrogen and nitrite in source water and finished water of Neijiang No.2 Water Works both exceeded the related standards, the levels of nitrate met the requirement of the Sanitary Standard for Drinking Water (2001) during the period from the first day to the third day after the end of discharge of industrial wastewater from contamination source. From the forth day to the 25 th day, Neijiang No.2 Water Works was changed to be supplied by the Five-star reservoir,and produced safe and sanitary finished water meeting the requirement of the Sanitary Standard for Drinking Water (2001). Conclusion This wastewater contamination accident seriously deteriorates the quality of drinking water in Jianyang and Neijiang.

[Objective]To introduce the treatment and result of meniscal cyst by arthroscopic surgery.[Method]During the period between 1997 and 2005,20 patients suffered from meniscal cysts have been treated by arthroscopic surgery in Xijing Hospital.Meniscal cysts were excised in all cases with shaver under arthroscopic visualization,whereas outside-in suture repair of meniscus was undergone in 8 cases to close the lacuna and repair the meniscal peripheral free which were resulted from remoral of cyst.The patients were followed up from 3 to 42 months with an average of(18.7?12.2)months.The patients were assessed pre-and post-operatively by the Lysholm score and Glasgow Assessment.[Result]Sixteen of total 20 patients were followed up.Meniscal cyst recurred in a patient one year after operation.Lysholm score of the 16 patients showed a significant improvement from(71.2?8.7)pre-operation to(97.1?2.9)post-operation(t=32.948,P

[Objective]To explore the method of rehabilitation for serious open injury below knee.[Method]A fotal of 153 cases with serious open injury below knee were treated.In which 106 cases were primary repair,47cases were second repair,treated with 7 kinds of internal fixation methods,11 kinds of flap.[Result]A fotal of 132 cases were followed up from 6 months to 5 years after operation,the average time was 17 months,the postoperative satisfaction rate was 85.7%,the flap survival rate was good 25 cases with local necrosis,4 cases with non-union,8 cases with chronic infection,3 cases with osteomyelitis.[Conclusion]The improvement of the flaps enlarge the application and reduce the complication of the flaps.The study offer a reasonable method to repair serious open injury below knee.The proficiency microsurgical scissors and reasonable application of various tissue flaps was the key to repair the injury successfully.

[Objective]To discuss the clinical problems of meniscal transplantation based on 2 cases clinical practical results including the harvest and preserved methods of meniscal allograft,operative procedure,rehabilitation plan and side-effect,combining with literature review,so as to provide reference for further clinical practice and research.[Method]The meniscal allograft were perfumed with arthroscopic technique on 2 patients,younger than 50 years,suffering from severe meniscus tear or defect at the time of other procedures.The initial clinical results about relieving of symptoms,recovery of functions were evaluated after short time rehabilitations.[Result]There was no operative side-injury and signs of knee instability.The incisions healed well without exudation.The knee joint swelling disappeared in 6 weeks.Rejection reaction was not observed in all of laboratory tests.The VAS were 8,6 preoperatively and 3,2 postoperatively.The processes of rehabilitation were normal.[Conclusion]Considering the difficult in maintaining severe tear meniscus and the characteristics of immune evasion of meniscus,meniscal allograft may be expectable option in the future.Very many researches have provided theoretical base.The results of long-time following-up should be further observed and wany clinical problems need to be further solved.

The characteristics and purpose of treatment of tibiofibular comminuted fracture is reviewed in this paper.The present points of view is that tibiofibular comminuted fracture is a kind of smash fracture,that is valuable for restoring its tubular structures.The appearance of a series of new type plates and external fixations represents the trend of researching.The combination of internal/external-fixation and sequential treatment is waited for more research.