Objective In acidity condition, arsenic and mercury can react on newly produced H2 to produce hydride which can be detected by atomic fluorescence spectrometry. The goal of the present paper is to establish a method to determine arsenic and mercury of the water at one time. Methods A quantitative method for determination by hydride generation-atomic fluorescence spectrometry was used .The electric current of the arsenic and mercury lamp were all 80 mA. The ratio of main arsenic electric current with the assist one was 50:30. 1% KBH4 of 0.2% NaOH solution were used as the reducers. Results The linear range of the arsenic was 0-10 ?g/L with correlation coefficient r=0.999 9. The RSDs and recovery rates of the arsenic were 2.0%-5.3% and 84%-113% respectively. The mercury showed a good linear result in the range of 0-5 ?g/L either, and the correlation coefficient was 0.999 3. The RSDs and recovery rates of the method were 1.9%-5.7% and 84%-107% respectively. Conclusion This method is simple, rapid, sensitive and accurate. It can determine arsenic and mercury in water at one time.

BACKGROUND:Hematopoietic stem cel transplantation is a promising treatment for a variety of malignant and nonmalignant disorders. But noninfectious pulmonary complications folowing hematopoietic stem cel transplantation are a major cause of morbidity and mortality in these patients. And the diagnosis and treatment are difficult. OBJECTIVE: To review the noninfectious pulmonary complications after hematopoietic stem cel transplantation in terms of onset time, risk factors, clinical manifestations, characteristics of the high-resolution CT, histopathological measurement, related genes and treatment options. METHODS: A computer-based search of VIP, PubMed and Sciencedirect databases was performed for articles related to noninfectious pulmonary compli cations after hematopoietic stem cel transplantation published from January 2005 to October 2014. The key words were “HSCT, pulmonary complications, non-infectious, pirfenidone” in Chinese and English in the title and abstract. Finaly, 31 articles were included in result analysis. RESULTS AND CONCLUSION: The incidence of noninfectious pulmonary complications after hematopoietic stem cel transplantation has become more and more, with atypical clinical manifestations and limited diagnosis and treatments. According to the different clinical onset time, diagnostic criteria, clinical manifestations and the appropriate laboratory tests, clinicians can make early diagnosis and early intervention. Especialy, the usage of high-resolution chest CT and bronchofiberscope for bronchoalveolar lavage as wel as the timely drug administration can improve the survival rate of patients with noninfectious pulmonary complications after hematopoietic stem cel transplantation.

OBJECTIVE:To study the effect of kallidinogenase on senile diabetic nephropathy.METHODS:95senile cases with diabetic nephropathy treated with the basic therapy were randomly divided into treatment group(plus kallidino?genase)and control group(plus aspirin),the proteinuria ejection level,hemorheological parameters and blood lipid level of2groups in24hours were investigated before and after treatment.RESULTS:As compared with prior treatment and the coutrol group,the proteinuria ejection level decreased significantly,the hemorheology improved and the blood lipid level decreased in the treatment group.CONCLUSION:Kallidinogenase is effective to some degree in the prevention and cure of senile diabetic nephropathy and diabetic macroangiopathy.

Objective To establish the analytical methods for determining the contents of tannins in different vegetative organs of Abutilon indicum (L.) Sweet. Methods The content of tannins, expressed as gallic acid equivalents, was measured by using the phospho molybdenum tungstic acid-casein colorimetry. Results The good linearity between concentrations and absorbances of gallic acid was found in the range of 2～10 mg/L, the average recovery was 99.63% and RSD was 1.08%, the contents of tannins in leaves, stems and roots of Abutilon indicum (L.) Sweet were 0.34%, 0.16% and 0.27% respectively. Conclusion The proposed method is sensitive, precise, accurate and reproducible, and it is suitable for the determination of tannins in Abutilon indicum (L.) Sweet.

Objective To compare the scale of the spinal bones removed by microendoscopic discectomy (MED) and interlaminal fenestration approach discectomy (IFAD),and their postoperative influence on the lumber degeneration in patients with lumber disc herniation.Methods Totally 76 cases of MED and 80 cases of IFAD were enrolled in this study.The scale of the spinal bones resected were compared by measuring the width and height of the bone window,as well as the percentages of the removed superior and inferior articular processes by using X-ray.To compare the efficacy of the two procedures,Nakai criteria was employed. The height and angle of intervertebral space and intervertebral displacement were detected preoperation,and then were followed up after the surgery.Results Both the width and height of the bone window and the percentages of the removed superior and inferior articular processes of the MED group was significantly less than those in the IFAD group (P=0.000);whereas,no marked difference was found in the scale of disc removal between the two group[(1.75?0.58) g vs (1.95?0.77)g,t=-1.825,P=0.070].The outcomes of the two group was similar,the rate of excellent and good outcomes of the MED and IFAD groups were 86.8% and 88.8%, respectively (Z=-0.184,P=0.854).In the MED group,the intervertebral space was reduced by 30%?18%,which were similar to that in the IFAD group 34%?15%(t=-1.246,P=0.215);3 cases of the MED group and 5 of the IFAD group had intervertebral instability after the surgery (x~2=0.425,P=0.515).Conclusions Both MED and IFAD are effective for lumbar disc herniation.However,MED shoud be the first choice for the disease,because the procedure causes less trauma.

Based on the survey of the learning preference of Kunming Medical College students,we analyze relationship between students’learning style and personalities.In order to match different learning style,teachers must find some strategies in their English teaching.

ObjectiveToobserve the killing effect of herpes simplex virus thymidine kinase/ganciclovir (HSV tk/GCV) suicide gene therapy system on human pancreatic carcinoma cell line. MethdsHSV tk gene was constructed into a retroviral vector pDOR neo. The recombinant plasmid pDOR tk neo was transfected into the retroviral packaging cell PA317. Finally HSV tk gene was transferred into human pancreatic cancer cell line PC 2. The sensitivity to GCV and bystander effect of PC 2/tk cells was tested in vitro. Antitumor effects were also observed after the administration of GCV in nude mice bearing tumor derived from PC 2/tk cells. ResultsHSV tk gene was successfully integrated into host cell. The killing effect of GCV on PC 2/tk cells was in a dose depandent and time depandent manners. The PC 2/tk cells showed a significant "bystander effect". Intraperitoneal injection of GCV postponed the formation of the implanted tumors. ConclusionPancreatic cancer cells expressing HSV tk can be effectively killed by GCV both in vitro and in vivo.

The characteristics and purpose of treatment of tibiofibular comminuted fracture is reviewed in this paper.The present points of view is that tibiofibular comminuted fracture is a kind of smash fracture,that is valuable for restoring its tubular structures.The appearance of a series of new type plates and external fixations represents the trend of researching.The combination of internal/external-fixation and sequential treatment is waited for more research.

Objective To investigate a water contamination accident caused by industrial wastewater during Feb 27th-29th ,2004,in Tuojiang river, analyze the variation of sanitary quality of drinking water during and after the accident. Methods After the occurrence of the accident,consecutive surveillance and analyses were done to discover the variation of the levels of amino-nitrogen,nitrite and nitrate in source water and finished water of Jianyang Water Works and Neijiang No 2 Water Works,which located at the lower reaches of Jianyang. Results The levels of amino-nitrogen in source water and finished water of Jianyang Water Works reached the first peak [(47.04?2.48)?(45.97?2.37)mg/L]at the 3rd day(March 3rd) after the end of discharge of industrial wastewater from contamination source,decreased at the 4th day ,reached the second peak at the 5th day[(42.26?2.88)?(39.50?2.64)mg/L], then decreased gradually, presented the normal levels at the 11th day[(0.54?0.10)?(0.30?0.12)mg/L]. The levels of nitrite in source water and finished water of Jianyang Water Works reached the first peak [(2.09?0.23)?(0.91?0.15)mg/L] at the 2 nd-3rd day after the end of discharge of industrial wastewater from contamination source,decreased at the 4th day ,reached the second peak at the 5th day[(2.18?0.33),(0.61?0.16)mg/L], then decreased gradually,presented the normal levels at the 11th day[(0.16?0.07)?(0.02?0.01)mg/L]. The levels of nitrate in source water and finished water of Jianyang Water Works(3.56～9.15 mg/L) didn't exceed the standard ruled the Sanitary Standard for Drinking Water (2001) throughout the duration of accident. The levels of amino-nitrogen and nitrite in source water and finished water of Neijiang No.2 Water Works both exceeded the related standards, the levels of nitrate met the requirement of the Sanitary Standard for Drinking Water (2001) during the period from the first day to the third day after the end of discharge of industrial wastewater from contamination source. From the forth day to the 25 th day, Neijiang No.2 Water Works was changed to be supplied by the Five-star reservoir,and produced safe and sanitary finished water meeting the requirement of the Sanitary Standard for Drinking Water (2001). Conclusion This wastewater contamination accident seriously deteriorates the quality of drinking water in Jianyang and Neijiang.

[Objective]To introduce the treatment and result of meniscal cyst by arthroscopic surgery.[Method]During the period between 1997 and 2005,20 patients suffered from meniscal cysts have been treated by arthroscopic surgery in Xijing Hospital.Meniscal cysts were excised in all cases with shaver under arthroscopic visualization,whereas outside-in suture repair of meniscus was undergone in 8 cases to close the lacuna and repair the meniscal peripheral free which were resulted from remoral of cyst.The patients were followed up from 3 to 42 months with an average of(18.7?12.2)months.The patients were assessed pre-and post-operatively by the Lysholm score and Glasgow Assessment.[Result]Sixteen of total 20 patients were followed up.Meniscal cyst recurred in a patient one year after operation.Lysholm score of the 16 patients showed a significant improvement from(71.2?8.7)pre-operation to(97.1?2.9)post-operation(t=32.948,P