In this paper, the traditional fault tree analysis method is presented, detailed instructions for its application characteristics in medical instrument maintenance is made. It is made significant changes when the traditional fault tree analysis method is introduced into the medical instrument maintenance: gave up the logic symbolic, logic analysis and calculation, gave up its complicated programs, and only keep its image and practical fault tree diagram, and the fault tree diagram there are also differences: the fault tree is no longer a logical tree but the thinking tree in troubleshooting, the definition of the fault tree's nodes is different, the composition of the fault tree's branches is also different.
Algorithms ; Durable Medical Equipment ; standards ; Maintenance

Due to the high technology and the complexity of medical equipment, as well as to the safety and effectiveness, it determines the high requirements of the medical equipment maintenance work. This paper introduces some basic methods of medical instrument maintenance, including fault tree analysis, node method and exclusive method which are the three important methods in the medical equipment maintenance, through using these three methods for the instruments that have circuit drawings, hardware breakdown maintenance can be done easily. And this paper introduces the processing methods of some special fault conditions, in order to reduce little detours in meeting the same problems. Learning is very important for stuff just engaged in this area.
Equipment and Supplies, Hospital ; standards ; Maintenance ; methods ; Safety

Objective: To improve the method of the international popular traditional CBM, and apply it to medical equipment maintenance. Methods: The monitor equipment real-time monitoring was replace by operating personnel’s real-time monitoring and by engineering technical personnel’s professional inspection regularly. The engineering and technical personnel replace experience with data, regularly test data with continuous data, and experience and reasoning of fuzzy with data processing and calculation of the precise results when analyzing the monitoring equipment unit, so as to achieve the purpose of CBM. Results:Compared with the PM mode, CBM mode’s maintenance workload and downtime maintenance reduce more than 30%and maintenance funds spending save 20~30%. Relative to BM mode, the quality success rate of medical instrument improves more than 5 percent and the personal safety hidden danger has fallen sharply. The medical benefit and economic benefit have been improved. Conclusion:It is effective and feasible to introduce the CBM mode to medical equipment maintenance.

Objective:To complete the medical equipment''s quality control on the premise of the lack of professional testing equipment.Methods: According to the working principle of instrument, to come up with corresponding simple and effective way to carry on the daily quality inspection, and make a qualitative conclusion that whether the instrument is qualified.Results:Effectively to ensure the safety and quality of medical equipment, medical equipment each year the average measurement test pass rate above 95% (bureau of quality and technical supervision measurement test results per year); perfectness ratio is around 98%.Conclusion: In the case of lack of professional testing equipment, through the integrated use of various effective means, can do the qualitative check that whether the performance of all sorts of equipment is qualified , achieve the goal of quality control.

Gansu province is a high incidence area of gastric cancer,and the mortality caused by gastric cancer ranks first in China.A safe and effective treatment of gastric cancer is the key point in the research.In recent years,tumor-targeted therapy has become a new research direction along with the gradually increased understanding of the molecular biology of cancer mechanism.Several studies have shown that increased expression of cyclooxygenase (COX)-2 plays an important role in the occurrence and development of malignant tumor.Celecoxib,a non-steroidal anti-inflammatory drug,is a novel COX-2 selective inhibitor which can significantly reduce the incidence of gastrointestinal malignancy,and inhibit tumorigenesis through inhibiting tumor growth and tumor angiogenesis,inducing the apoptosis of tumor cells,reversing multidrug resistance,enhancing the cytotoxicity and radiosensitivity of chemotherapy drugs,and producing synergistic effects with combining chemotherapy and radiotherapy.Therefore,celecoxib has the potential to be the treatment of choice for gastric cancer.

The present study is to investigate the protective actions of guggulsterone against the cytotoxicity produced by exposure to hydrogen peroxide (H2O2) in PC12 cells. It was evaluated by MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide] reduction assay, lactate dehydrogenase (LDH) release assay, and the release of nitric oxide (NO). ROS and Ca2+ in cells were evaluated by DCFH and Fura 2-AM, respectively. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine 123 (Rh 123). Apoptosis and morphological alteration in PC12 cells were monitored with flow cytometry and electric microscope. Vitamin E, a potent antioxidant, was employed as a comparative agent. The results showed that preincubation of PC12 cells with guggulsterone (0.1-10 μmol·L-1) prevented cytotoxicity induced by H2O2. Extracellular accumulation of LDH, NO and intracellular accumulation of ROS, Ca2+ resulting from H2O2 were significantly reduced by guggulsterone. Incubation of cells with H2O2 caused a marked decrease in MMP, which was significantly inhibited by guggulsterone. The percentage of H2O2-induced apoptosis in PC12 cells was 24.3%, and decreased in the presence of guggulsterone (0.1-10 μmol·L-1) by .8.4%, 15 .9%, 11.8%, respectively. Guggulsterone exhibited comparable potency against oxidative stress induced by H2O2 in PC12 cells as that of vitamin E. The present findings showed that guggulsterone attenuated H2O2-induced cytotoxicity, extracellular accumulation of LDH and NO, intracellular accumulation of ROS and Ca2+, loss of MMP, and apoptosis, which may represent the cellular mechanisms for its neuroprotective action.

BACKGROUND: As a group of primary cells,stem cells have the potentials of self-renewal and multi-directional differentiation, and can replace the damaged cardiomyocytes to construct new vessels, and enable the asthenic heart to obtain the blood pumping function again.This study summarized the progress of stem cell transplantation in treating cardiovascular diseasesDATA SOURCES:A computer-based online search of Pubmed database was undertaken to identify English articles published from January 2000 to December 2005, the keywords were "stem cell transplantation, cardiovascular diseases".STUDY SELECTION: The articles were primarily checked, and the references of each literature were looked up. Inclusive criteria:articles correlated with the classification and the transplanted pathways of stem cells,the present status,problems and prospect of its basic and clinical application in treating cardiovascular diseases. Exclusive criteria: repetitive study or Meta analysis.DATA EXTRACTION:Totally 50 relative literatures were collected, and 31 of them accorded with the inclusive criteria,and the other 19 were excluded due to the old or repetitive contents. Of the 31 involved literatures, 19 were about the classification of stem cells used in cardiovascular system, 1 about the transplanted pathways of stem cells, 8 about the application of stem cells in treating cardiovascular diseases, and 3 about the existing problems and prospect.DATA SYNTHESIS:With the continuous improvement of living level and aging, cardiovascular diseases have become the main causes for death and disability in modern society.Stem cells have the potentials of multi-directional differentiation, and have become the main seed cells for cell replacement therapy, and provided important investigative and applicating field for the treatment of cardiovascular diseases. At present, there are many stem cells to be used in cardiovascular system, including embryonic stem cells, umbilical cord blood stem cells, peripheral blood stem cells, bone marrow stem cells, skeletal muscle satellite cells, myocardial stem cells, adipose tissue-derived stem cells, etc. The common pathways for stem cell transplantation are direct injection into myocardium,intracoronary artery injection and intravenous injection.Stem cell transplantation can treat cardiovascular diseases by promoting angiogenesis and myocardial regeneration, decreasing myocardial apoptosis and reducing myocardial collagen, then ameliorate the cardiac function, and breakthrough and progress have been made in both animal models and clinical studies. However, problems sill exist to be solved, and there is agreat distance to the routine clinical application.CONCLUSION: Stem cells are the important fields of the basic research and clinical application for preventing and treating cardiovascular diseases,thus it will provide powerful theoretical and practical supports for the more effective prevention and treatment of cardiovascular diseases to solving the key points and difficulties in cardiovascular diseases.

To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C, we soaked the crystal of 6-phosphate-beta-glucosidase (Bg1A) in the liquor containing p-nitrophenyl-beta-D-glucopyranoside-6-phosphate (pNPbetaG6P). The diffraction data at 2 A resolution was collected and an intact and unambiguous electron density map of pNPbetaG6P was obtained. These results provide an effective method for the research of cryoenzymology and the intermediate state of enzyme-substrate complex in the future.
Cold Temperature ; Crystallization ; Glucosidases ; chemistry ; metabolism ; Substrate Specificity ; X-Ray Diffraction

[Objective]To explore the method of rehabilitation for serious open injury below knee.[Method]A fotal of 153 cases with serious open injury below knee were treated.In which 106 cases were primary repair,47cases were second repair,treated with 7 kinds of internal fixation methods,11 kinds of flap.[Result]A fotal of 132 cases were followed up from 6 months to 5 years after operation,the average time was 17 months,the postoperative satisfaction rate was 85.7%,the flap survival rate was good 25 cases with local necrosis,4 cases with non-union,8 cases with chronic infection,3 cases with osteomyelitis.[Conclusion]The improvement of the flaps enlarge the application and reduce the complication of the flaps.The study offer a reasonable method to repair serious open injury below knee.The proficiency microsurgical scissors and reasonable application of various tissue flaps was the key to repair the injury successfully.

Literatures about patella fracture fixation in recent years are analyzed retrospectively in this paper.Its characteristics and therapeutic purpose are investigated.A series of new type of internal fixations and external fixations are emphasized.