TCM experimental teaching has a very important position in university training process, in which the laboratory standardized management and the strengthening of the teaching quality monitoring play important roles. According to the the experimental teaching reforms and requirements enacted in recent years by the Ministry of Education of the People’s Republic of China, Hunan TCM University has conducted a series of standardized management of exploration and practice in the laboratory.

Objective To evaluate the effect of preconditioning with penehyclidine hydrochloride (PHC)on mitochondria-dependent pathway of apoptosis during myocardial ischemia-reperfusion(I∕R)in rats. Methods Twenty-four healthy adult male Wistar rats were allocated into 3 groups(n=8 each)using a random number table: sham operation group(group S), I∕R group and PHC preconditioning group (group P). Myocardial I∕R was produced by occlusion of left anterior descending branch of coronary artery for 30 min followed by 3 h reperfusion. PHC 10 mg∕kg was injected via the tail vein at 30 min before oc-clusion in group P. The rats were sacrificed at the end of reperfusion, hearts were removed and left ventric-ular specimens were obtained for determination of apoptosis in cardiomyocytes(by TUNEL), and apoptosis index(AI)was calculated. Mitochondria and cytoplasm were isolated for detection of mitochondrial mem-brane potential(MMP, by JC-1 method)and expression of Bcl-2, cytochrome c(Cyt c)in mitochondri-a and cytoplasm and activated caspase-3 in myocardial tissues(by Western blot). Results Compared with group S, AI was significantly increased, the expression of Bcl-2 and mitochondrial Cyt c was down-regula-ted, MMP was decreased, and the expression of Cyt c in cytoplasm and activated caspase-3 was up-regula-ted in I∕R and P groups(P<0. 05). Compared with group I∕R, AI was significantly decreased, the ex-pression of Bcl-2 and mitochondrial Cyt c was up-regulated, MMP was increased, and the expression of Cyt c in cytoplasm and activated caspase-3 was down-regulated in group P(P<0. 05). Conclusion The mechanism by which PHC preconditioning attenuates myocardial I∕R injury is related to inhibiting mitochon-dria-dependent pathway of apoptosis in rats.

Objective To explore the influencing factors of serum uric acid (UA) level and its relationship with SLC2A9 gene polymorphism. Methods A total of 2000people in the health examination center of Yangpu District Central Hospital were selected to examine their blood pressure, blood sugar, blood lipid and other biochemical indicators. The single nucleotide polymorphism (SNP) locus rs2241480 of SLC2A9 gene was detected and analyzed. According to UA level, UA was divided into high UA group (n=217), middle UA group (n=1705) and low UA group (n=78). The biochemical indexes and SLC2A9 genotype of each group were compared, and the relationship between UA and SLC2A9 gene polymorphism was analyzed. Results The prevalence of hyperuricemia (HUA) was 10.85% in physical examination population, 12.92% in males, which was significantly higher than 8.48% in females (P﹤0.05). With the increase of UA level, body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), blood urea nitrogen (BUN), creatinine (Cr) increased significantly, and high density lipoprotein (HDL-C-c) increased significantly.) there was a significant decrease, with statistical significance (P﹤0.05). The genotyping of rs2241480 locus in different UA levels showed significant difference (P﹤0.05). Male (OR=1.99), BMI (OR=3.01), SBP (OR=3.77) were independent risk factors for HUA, while HDL-C (OR=0.27) and rs2241480 locus genotype (CC, OR=0.41) were protective factors (P﹤0.05). Conclusion Traditional cardiovascular risk factors such as blood pressure and lipid are independent risk factors for UA level. SLC2A9 gene polymorphism may be associated with the occurrence of HUA.