Objective: To study the relationship between the renin (REN) gene G10631A, angiotensinogen (AGT) gene T704C, C521T mononucleotide polymorphisms and cerebral infarction. Methods: One hundred eighty patients with cerebral infarction and 130 healthy controls were recruited. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the REN G10631 site, AGT T704 and C521 site genotype and allele. The differences of the genotype and allele frequencies in both groups were compared. Logistic regression was used to analyze the risk factors for cerebral infarction. The haplotype structure of the population was analyzed in order to find cerebral infarction related polymorphism combination in this population. Results: Circled digit oneThe REN 10631 AA genotype frequency (31.7%) and the A allele frequency (49.4% ) in the cerebral infarction group were high-er than 10.0% and 30.3% in the healthy control group (P<0.05). Circled digit twoThe AGT 704 CC genotype frequency (63.3% ) and C allele frequency (79.7% ) in the cerebral infarction group were higher than 34.6% and 61.2% in the healthy control group (P<0.05). Circled digit threeThe AGT 521TT genotype frequency (21.7% ) and T allele frequency (27.8%) in the cerebral infarction group were higher than 6.9% and 11.9% in the healthy control group (P<0.05). Circled digit fourMultivariate Logistic regression analysis showed that REN 10631AA genetype, AGT 704CC genetype, and AGT52ITT genetype could increase the probability of cerebral infarction. The relative risk (OR) of the onset was 2.617, 2.699, and 3.362, respectively (P < 0.05); Circled digit fiveThe distribution frequency of the haplotype 521T-10631A-704C in the cerebral infarction group was higher than that in the healthy control group (P = 0.000). Conclusion: REN gene 10631AA genetype and A allele, ACT 704CC genetype and C allele, and AGT 521TT genetype and T allele may be the susceptible factors of cerebral infarction. Haplotype 521T-10631A-704C may be the genetic risk factors for the onset of cerebral infarction.

Through analyzing the data of ectopic thyroid glands, to know this kind of congenital lesion deeply. In all the cases, parathyroid gland types were more than aberrant thyroid types; the majority cases were single ectopic thyroid; most of ectopic thyroid were present in the neck and mouth; local mass was the most common symptom; adenoma was often associated with ectopic thyroid and there was a high proportion of combined malignant; most cases could not be correctly diagnosed.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Choristoma ; pathology ; Female ; Humans ; Infant ; Male ; Middle Aged ; Thyroid Gland ; pathology ; Young Adult

Objective To study the hope level in patients with high-risk gestational trophoblastic neoplasm and to explore its related factors.Methods 83 cases of patients with high-risk gestational trophoblastic neoplasm were collected and investigated with Herth Hope Scale,Social Support Rating Scale (SSRS) and self-designed questionnaire.The results underwent analysis.Results The hope level of most patients underwent investigation was at the moderate level,among which the positive attitude for future and reality scored (10.80 ± 1.77),adopting positive action scored (10.30 ± 2.36),keeping close relationship with others scored (9.63 ± 2.13),the total scored (30.74 ± 4.82).The hope oevel was positively correlated with rigor personality (r=0.254),but negatively correlated with neuroticism (r=0.237),the difference was statistically significant.Conclusions The hope level of patients with high-risk gestational tropoblastic neoplasm is above the moderate level and is correlated with many factors.Doctors and nurses should evaluate carefully enough and take nursing measures to improve the hope level and life quality.

Objective To observe the effect of the outpatient mobile infusion system in the department of infectious outpatient night infusion.Methods From July to December in 2012,520 patients in department of infectious outpatient infusion in our hospital were selected and were divided into the observation group (using outpatient mobile infusion system)and the control group (adopting conventional infusion) with 260 cases in each group.The infusion effect was compared between two groups.Results The average occupancy per patient care time in the observation group was less than the control group,the difference was statistically significant.The satisfaction degree in the observation group was higher than that of the control group,the difference was statistically significant.There was no nursing error occurred in both of the observation group or the control group.Conclusion The use of outpatient mobile infusion system can significantly improve the nurses work efficiency,ensure nursing safety,improve quality of care and patients' satisfaction degree.

Objective To investigate the effects of desflurane and/or nitric oxide (NO) on the lung injury induced by ischemia-reperfusion ( I/R) during cardiopulmonary bypass (CPB) in children with pulmonary hypertension secondary to congenital heart disease (CHD) .Methods Forty children with CHD and pulmonary hypertension (24 male, 16 female) aged 0.6-3.7 yrs weighing 7.1-11.9 kg undergoing cardiac surgery under CPB were randomly divided into 4 groups ( n = 10 each): group control; group DES; group NO and group DES + NO. The patients were premedicated with oral midazolam 0.5 mg?kg-1. Anesthesia was induced and maintained with fentanyl and vecuronium. Radial artery was cannulated for MAP monitoring and blood sampling. Pulmonary catheter was placed under direct vision after chest was opened. The patients received inhalation of desflurane (1-1.3 MAC) (group DES)/NO (10-20 ppm) (group NO) /DES + NO (group DES + NO) immediately after pulmonary catheterization until the start of CPB. MAP, PAP, peak airway pressure (Ppeak) and compliance of respiratory system (Crs) were recorded at 5 min after induction (T0 ), 5 min before CPB (T1 ), 5 min after start of CPB (T2) and at the end of surgery (T3) . Blood samples were taken at T0 and T3 for determination of methemoglobin (Met-Hb), soluble intercellular adhesion molecule-1 (sICAM-1), XOD and MDA.Results The four groups were comparable with respect to age, M/F ratio, body weight, duration of CPB and type of operation performed. MAP was significantly decreased after inhalation of desflurane alone or desflurane + NO before CPB ( at T1) as compared to the baseline value at T0; while PAP was significantly decreased after inhalation of NO alone or NO+ desflurane before CPB (at T1) as compared to the baseline value. Ppeak was significantly lower while Crs was significantly higher at end of surgery (T3) in group NO and NO + desflurane than in control group. Blood sICAM-1 and MDA concentrations and XOD activity were significantly lower at the end of surgery (T3 ) in group DES, NO and DES + NO than in control group. Met-Hb was significantly increased at the end of surgery (T2) as compared to the baseline (T0) in group NO and DES + NO but was still within normal range. Conclusion Inhalation of desflurane and/or NO can ameliorate the lung injury during CPB in children with pulmonary hypertension secondary to CHD, in addition to inhibit the pulmonary hypertension.

Diabetic cardiomyopathy ( DC) is an independent complication of diabetes mellitus accompanied with cardiac metabolic imbalances ( increase of fatty acids and reduction of glucose) ,which would impair cardiac function and structure seriously. Peroxisome proliferator-activated receptors ( PPARs) are ligand-activated transcription factors belonging to the nuclear hormone receptor superfamily,and they could transcriptionally regulate energy metabolism and function. PPARs also play an important role in regulating metabolism in DC heart,and directly or indirectly affect cardiac function and structure.

Diagnosis, Differential ; Humans ; Intestinal Diseases ; etiology ; Intussusception ; etiology ; Pancreatitis ; complications ; diagnosis ; Purpura, Schoenlein-Henoch ; complications ; diagnosis

Objective To evaluate the application value of the one-stepdiabetes education management system in clinic. Methods The “one-step” diabetes education management system was construct.80 newly diagnosed patients with type 2 diabetes mellitus were selected and randomly divided into the control group and the experimental group with 40 patients in each group.The patients in the experimental group received “one-step”diabetes education management system by diabetes specialist nurses,while patients in the con-trol group received routine diabetic education.The fasting blood glucose (FBG),HbAlc,medical compliance behaviors and other indices were compared between two groups. Results FBG,HbAlc and medical compliance were better,and the risk score of complications was higher in the experimental group than those of the control group,there were significant differences between them. Conclusions Application of “one-step”diabetes education management system in treatment and nursing of patients shows maneuverability and practicality.It has clinical application value and deserves to be spread.

Vibrio parahaemolyticus has been considered as one of the most important foodborne bacterial pathogens.The loop-mediated isothermal amplification(LAMP) that amplifies DNA with high specificity and rapidity under an isothermal condition was applied for rapid detection of this pathogen for the first time.A set of four primers,two outer and two inner primers,was designed specifically to recognize the thermolabile hemolysin gene(tlh) of V.parahaemolyticus.The LAMP reaction mix was optimized.The most optimal reaction temperature and time of the LAMP assay for the tlh gene were 60℃ and 60min,respectively.Genomic DNAs from 28 bacterial strains including 14 V.parahaemolyticus strains were amplified using LAMP,and no amplicon was observed in other bacterial strains.The detection limit of this LAMP assay was around 90 fg of V.parahaemolyticus genomic DNA and 24 colonies forming units for pure cultures.In addition,this method was applied to detect artificially contaminated food samples,and the detection limit was 89 cfu/g for non-cultured artificially contaminated food samples.These results suggested that detection of V.parahaemolyticus by LAMP is an effective and low-cost procedure with high specificity and sensitivity that requires no specialized equipment.This assay is expected to become a valuable tool for rapid detection and identification of V.parahaemolyticus.

Objective To evaluate the usefulness of diffusion-weighted MR combined with routine T2 WI in finding the possible residual foci in uterine cervical cancers after radical chemo-radiation therapy.Methods This was a retrospective study including 25 consecutive cervical cancer patients who received hysterectomy after radical chemo-radiation therapy.All of them underwent MR examinations post-chemoradiation and just before operation.Images of T2 WI alone and those of T2 WI combining DWI were evaluated respectively by 2 senior radiologists,in order to decide whether there were residual tumors.ADC values were also measured.Taking the post-operation pathological results as the gold standard,the accuracies,sensitivities and specificities of T2 WI alone,T2 WI combining DWI,and ADC values were all calculated.Results In those 25 patients,9 were found with foci of residual cancer in operative pathology,while no cancer cells were found in the other 1 6 patients.The accuracy,sensitivity and specificity in finding the positive residual cancer using T2 WI alone were 56.0%,77.8% and 43.8%,comparing with 72.0%,66.7% and 75.0% in T2 WI combining DWI.The accuracy and specificity increased with statistical significance after combining DWI (P =0.01 6 for accuracy,P =0.031 for specificity),while the sensitivity decreased but did not reach statistically significant level (P =0.099).No difference in ADC values was found.Conclusion DWI can be used as a supplementary sequence in finding the existence of residual tumors of cervical cancer after radical chemo-radiation therapy.Routine T2 WI combing DWI increased the specificity and accuracy,but still facing the risk of decreasing sensitivity.