The development of thoracoscopic minimal invasive neonate surgery has brought unprecedented challenges for modern anesthesiology.Many factors including neonatal physiology,pathological changes and surgery itself,anesthetic techniques and intraoperative ventilation mode affect neonate perioperative safety and therapeutic efficacy.We analyze the reasons and solutions of complications by focusing on the perioperative hypoxemia,hypercapnia,ventilatory disorders and hypotension that may occur during artificial pneumothorax to improve the anesthetic safety of neonate minimal invasive surgery.

Objective To explore the characteristic of metabolic disorders between adolescent and childbearing period PCOS women with obesity or without obesity.Methods 80 PCOS patients were according to obesity divided into 52 patients with PCOS(BMI ≥25kg/m2) and 28 patients with non-obese PCOS (BMI ＜ 25kg/m2),and according to age divided into 36 cases of adolescent(age 14-19 years) and 44 cases of child-bearing age PCOS patients (age 20-32 years).The height,weight,blood pressure,oral glucose tolerance test (OGTT),insulin releasing test (IRT),serum lipid,liver function test were observed.Results (1)Compared with non-obese PCOS women,the obese PCOS women had significantly higher fasting blood insulin,HOMA-IR,fasting blood glucose,AUCG,serum lipid (TC,TG,LDL,ApoB),and the more serious impaired glucose tolerance(IGT).(2)Compared with adolescent PCOS women,the child-bearing period PCOS women had significantly higher AUCG,serum lipid,and more serious IGT.Conclusion The adolescent and child-bearing period PCOS patients always have different degree metabolic disorders.The obese PCOS women especially obese child-bearing period PCOS women may have increased risk of suffering from metabolic disorders in glucose and lipid.For adolescent PCOS women particularly obese ones,we should take early screening,diagnosis,intervention,to avoid the further development and long-term complications.

Objective To study the effectiveness of long-range electrocardio-monitoring system for aged people outside the hospital.Methods Long-range electrocardio-monitoring network system was adopted to perform monitoring 3 times every day for 320 retired leaders in Tianjin.Results The coincidence rate was 92.4%,and the morbidity rate 1.1%.Conclusion The long-range electrocardio monitoring network system can transfer patient's real-time information.Patients can be monitored by hospital at any time and in any place.

Objective Effects of different factors on shoot tip culture in vitro were studied by using Dioscorea bulbifera as test material to find the media suitable to the shoot tip differentiation of D.bulbifera and the method of virus delection.Methods Plant tissue culture method was used in shoot tip culture and the symptom observation method,instruction plant method,and RT-PCR method were used in virus detection of virus-free plantlets.Results The best disinfection method for D.bulbifera shoot tips was firstly disinfected for 30 s with 70% alcohol and then disinfected for 12 min with 0.1% HgCl2;It is better for D.bulbifera to cut shoot tips in 0.5 mm length after 37 ℃ heat treatment for 7 d;The best proliferation medium of D.bulbifera shoot tips was MS+KT 2 mg/L+NAA 0.5 mg/L;The best rooting medium of regeneration buds from D.bulbifera shoot tips was 1/2 MS+NAA 0.5 mg/L;The best matrix of regeneration plantlets from D.bulbifera shoot tips was perlite-vermiculite (2∶1);RT-PCR Method was primary mean to detect potato virus Y (PVY) of regeneration plantlets from D.bulbifera shoot tips,and average rate of PVY-free was 86.5% by RT-PCR.Conclusion The virus-free culture system of D.bulbifera shoot tips is established for the first time,providing a technological basis for the rapid propagation and factory production of D.bulbifera virus-free plantlets.

Objective Effects of different factors on proliferation and rooting of stems with a bud were studied by using Dioscorea bulbifera as test material to optimize the rapid propagation system of D.bulbifera virus-free plantlets.Methods Plant tissue culture method was used in shoot tip culture and rapid propagation study,and RT-PCR method was used in virus detection of virus-free plantlets.Results The best proliferation medium of D.bulbifera stems with a bud was MS+KT 2 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L;The best sucrose and agar concentration of D.bulbifera stems with a bud was 30 g/L and 0 g/L,respectively;The best rooting medium of D.bulbifera stems with a bud was 1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP_(333) 1 mg/L;The best transplanting matrix of regeneration plantlets from D. bulbifera stems with a bud was perlite-vermiculite(2:1);The best PP_(333) concentration of D.bulbifera regeneration plantlets for transplanting was 50 mg/L.Conclusion The rapid propagation system of D. bulbifera virus-free plantlets is established successfully for the first time,which provides a technological basis for factory production of D.bulbifera virus-free plantlets.

Aim To search for small molecule NGF mimetic s using a primary screening model.Methods Both rapid quantitati ve bioassay for NGF(-like) activity by measurement of neurite extension in NGF -induced PC12 cells and a following MTT technique to monitor cells alive were u sed.Results More than 1 600 compounds were tested using the se methods and several effective samples with lower cytotoxicity were found. One of them, the compound B,was in good dose-and time-effect relationship. Conclusion All above studies provide a useful model and chemical info rmation for further screen of neurotrophic mimetics and neural cell tumor induce rs.

Acidic leucine-rich nuclear phosphoprotein 32E (ANP32E) belongs to a family of acidic nuclear phosphoprotein 32, and participates in a broad range of biological processes. ANP32E is similar to ANP32A in structure. It has been suggested that the nueleo-cytoplasmic shuttling might be regulated by phosphorylation. ANP32E is able to inhibit the activity of PP2A. Recent findings indicate ANP32E acts as a tumor suppressing gene in mice fibrosarcoma, and is correlated with lung metastasis in the tumorigenesis of breast cancer. But the mechanism is not yet clear.

More and more evidence indicates that microvesicles (MVs) play a key role in cell-to-cell communication. The MVs are circular fragments of membrane released from the endosomal compartment as exosomes or shed from the cell surface membranes of most types. Components of donor cells are incorporated into MVs that contain bioactive lipids, proteins, genetic cargoes. MVs derived from stem cells may reprogram cells that survived in injury tissue and favor tissue regeneration by delivering their bioactive cargoes to influence the behaviors of recipient cells. Compared with mesenchymal stem cells (MSCs), MVs derived from MSCs were found to mimic the beneficial effects of these cells. These proregenerative effects mediated by MVs can be explained by the fact that MVs are enriched in bioactive lipids, anti-apoptotic and pro-stimulatory growth factors or cytokines, and deliver mRNAs, regulatory miRNAs and proteins that improve overall cell function. Therefore, it opens novel perspectives in exploiting these MVs in tissue regeneration and repair. In addition, the use of MVs instead of stem cells could represent a safe and potentially more advantageous alternative to cell-therapy approaches.
Exosomes ; Humans ; Regeneration ; Stem Cells ; cytology

Objective To explore the correlation of random spot albuminuria to creatinine ratio (ACR) and 24 h uri?nary protein, and the diagnostic value of ACR in women with hypertensive disorders complicating pregnancy (HDCP). Meth?ods A total of 584 women with HDCP (169 with gestational hypertension, 205 with mild pre-eclampsia, 173 with severe pre-eclampsia and 37 with chronic hypertension) and 2 038 normal pregnant women were included in this study. Changes of ACR, 24-h urinary protein and other biochemical indicators were compared between groups. Bivariate correlation analysis was used to analyze the relationship between ACR, 24-h urinary protein and other biochemical indicators. Receiver operat?ing characteristic curve (ROC) was used to analyze the sensitivity, specificity and optimal cut off value of ACR for predicting HDCP. Results (1)There were significantly higher levels of ACR, 24-h urinary protein, blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total cholesterol (TC) and low density lipoprotein cholesterol (LDL) and significantly lower val?ues of platelet (PLT), serum albumin and total protein in women with severe preeclampsia than those of other groups ( P <0.05). (2) There were positive correlation between ACR and 24-h urinary protein, BUN, Cr, UA, D-dimer, TC, LDL (P <0.05). (3) The optimal cut off value of ACR for predicting gestational hypertension, mild preeclampsia, severe preeclampsia (24-h urinary protein≥2 g,≥5 g) were 1.44, 10.48, 39.84, 94.91 g/mol separately. Conclusion There is positive correla?tion between ACR and 24 hour urinary protein. ACR can be used for diagnosing and estimating the severity degree of hyper?tensive disorders complicating pregnancy.

Objective:To study the effects of artesunate on K562, K562 /ADM cell apoptosis and NF-κB p65 expression in hu-man leukemia. Methods: The effect of artesunate with different concentrations on K562 and K562 /ADM cell apoptosis at various times was studied by a flow cytometer. The effect of artesunate with 15 μmol·L-1 on NF-κB P65 expression in K562 was explored u-sing a Western blot method. Results:Artesunate showed little effect on K562 cell apoptosis, while exhibited notable effect on K562 /ADM cell apoptosis. K562 /ADM cells were more sensitive than K562 to artesunate at the concentration of 7. 5 μmol·L-1 and 15μmol·L-1(P<0. 01), and after 4-hour treatment with 15 μmol·L-1 artesunate, K562 /ADM cell apoptosis was much higher than K562(P<0. 01). Artesunate could reduce leukemia cell p65 expression at the concentration of 15 μmol·L-1. Conclusion:Artesu-nate can induce the apoptosis of leukemia cells by down-regulating the expression of p65.