Animals ; Humans ; Interleukin-6 ; physiology ; Liver Cirrhosis ; physiopathology ; Mice ; Pulmonary Fibrosis ; physiopathology ; Rats

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Psoriasis ; diagnosis ; therapy

To generate recombinant adenovirus expression vector of human Sema4C gene and observe its expression in mouse myoblasts cell line C2C12 for ensuring easy access to investigate the role of Sema4C gene during myogenesis. The recombinant plasmid was packaged and amplified after being transfected in HEK293 cells through Lipofectamine. After infecting C2C12 myoblasts with recombinant adenovirus vector, the adenoviral infection efficiency was determined by confocal microscope which showed that the expression of green fluorescence could be detected at 12h and then reached peak at 24h after recombinant adenovirus infection. The infection efficiency was almost 100% confirmed by FACS examination. Detection of WB indicated that the expression of Sema4C in C2C12 of recombinant adenoviral infection group was significantly higher than that of the control group (P

Up to now, a variety of microRNAs have been found in a number of studies, that specifically expressed in retinal neuroepithelial, lens, cornea and retinal pigment epithelium, in which miR-126 plays a certain role in the proliferation of tumor cells, the development of thymus lymphocytes and cardiovascular diseases.Some researches show that miR-126 has certain correlations with the formation of corneal neovascularization, the development of diabetic retinopathy, and the immune system related eye disease.In this paper, the current miR-126 in the role of eye disease mechanism and research progress were reviewed.

Background Sphingosine-l-phospate (S1P) is a bioactive lipid and important messenger molecule in cells.It participates in the regulation of many biological processes,such as cell proliferation,migration,survival,differentiation,apoptosis,etc.Hypoxia is a trigger factor of choriod neovascularization (CNV) and pathological basis of many diseases,and retinal pigment epithelial (RPE) cells are involved in formation of CNV.However,the effects of S1P on proliferation and apoptosis of RPE cells are below understood.Objective This study was to investigate the influence of S1P on proliferation and apoptosis of human RPE cells under hypoxic conditions.Methods Human RPE cells line-D407 cells were cultured and passaged and generation 3-5 cells were used and divided into 6 groups.The cells were regularly cultured in the blank control group using DMEM containing 10％ fetal bovine serum.CoCl2(200.00 μmol/L) was added into the colture medium for 2 hours in the hypooxic group.S1P of different concentrations (0.01,0.10,1.00,10.00 μmol/L) were added in culture medium 2 hours after the affection of 200.00 μmol/L CoCl2.The proliferative values of the cells were detected using WST-1 method as the absorbance (A value) and the proliferative rate of different groups were calculated.The apoptosis of the cells was assayed by Hoechst staining.The results were compared among different groups.Results Cultured cells showed the round-like in shape with clear nuclei and pigment.The proliferative values (A value) was 0.91 ±0.08,0.37±0.09,0.46±0.08,0.52±0.09,0.61 ±0.06,0.70±0.10 in the blank control group,hypoxic group and 0.01,0.10,1.00,10.00 μmol/L S1P groups,respectively,with a significant difference among the groups (F=21.104,P=0.000),and A values in various S1P groups were higher than those in the hypoxiac group (all at P＜0.05).The proliferative rate was gradually raised with the increase of dose of S1P.Hoechst staining exhibited a few apoptosis cells in the blank control group,but in the hypoxic group,a lots of apoptosis cells were seen with the light-blue nuclei and condensable chromatin.However,the number of apoptosis cells was significantly decreased in various concentrations of S 1 P groups.The apoptosis rates were (1.21 ±0.08) ％,(8.99 ±0.09) ％,(6.60 ±0.08) ％,(5.95 ±0.09) ％,(4.81 ± 0.06)％ and (3.96±0.10)％ in the blank control group,hypoxic group and the 0.01,0.10,1.00,10.00 μmol/L S1P groups,respectively,with a significant difference among the groups (F =25.070,P =0.000).Compared with the hypoxia group,the cellular apoptosis rates of various S1P groups were lower (all at P＜0.05).Conclusions Under the hypoxia condition,S1P can promote the proliferation of human RPE cells and inhibit apoptosis.

OBJECTIVE: To investigate the effects of microwave radiation on learning and memory abilities in mice, and to study pilose antler peptide's intervention. METHODS: Fifty mice were divided into five groups randomly, designated as control group, radiation group, pilose antler peptide (25, 50, and 100 mg·kg-1) groups. Learning and memory impairment model in mice was established by microwave radiation of 2 450 MHz average surface power, 10.0 mW·cm-2 for 90 min every day for 28 d.The radiation rats were treated with low-, mid-, and high-dose (25, 50, and 100 mg·kg-1) pilose antler peptide by sc injection for 28 d. The learning and memory ability of mice was determined by avoiding darkness experiment and Y maze experiment.The contents of S100B, tumor necrosis factor-α(TNF-α), interleukin-10(IL-10), malondialdehyde (MDA), and nitric oxide(NO) in the brain of mice were determined respectively after the behavioral experiments. RESULTS: Compared with control group, radiation group could shorten the latency of avoiding darkness experiments, increase the numbers of errors both in avoiding darkness experiment and in Y maze experiment. Radiation group could rise the contents of S100B, TNF-α, IL-10, MDA and NO in the brain of mice (P<0.05 or P<0.01). Compared with radiation group, pilose antler peptide (50, 100 mg·kg-1) groups could lengthen the latency of avoiding darkness experiments, significantly shorten the numbers of errors both in avoiding darkness experiment and in Y maze experiment, and reduce the contents of S100B, TNF-α, MDA and NO, increase the content of IL-10 in the brain of mice (P<0.05 or P<0.01). CONCLUSION: Pilose antler peptide could significantly perfect the learning and memory ability of mice exposed to microwave radiation. The mechanism may be related to its anti-oxidative actions by anti-inflammatory action, further lowering neurotoxic effects of NO.

Objective To investigate the level of serum retinol-binding protein 4 (RBP4) and related factors in chronic hepatitis C (CHC). Methods Fifty-six patients with CHC (CHC group) and 35 healthy volunteers (control group) were selected. Serum RBP4 level was measured by ELISA method.Fasting blood glucose ( FBG ), triglycerides (TG), total cholesterol ( TC ), alanine aminotransferase (ALT),γ-glutamyl transpeptidase (γ-GT) were measured, HCV-RNA level was tested by qualitative polymerase chain reaction(q-PCR). Results There were no significant difference in FBG, TC,TG, γ-GT between two groups (P ＞ 0.05 ). Serum RBP4 level in CHC group [(33.38 ± 6.43 ) mg/L] was higher than that in control group [(26.11 ± 3.35) mg/L](P＜ 0.01),the CHC patients with ALT normal (26 cases) had significantly higher RBP4 level [( 38.96 ± 4.09) mg/L] compared with ALT abnormal [30 cases, ( 28.53 ± 3.43 ) mg/L](P ＜ 0.01 ). ALT level was negative with RBP4 in CHC group (r = -0.6368, P ＜ 0.05 ). Conclusion Serum RBP4 level is significantly associated with CHC and negatively correlated with ALT level,but not associated with FBG, TC,TG, γ-GT and HCV-RNA.

Objective To investigate the application and clinical significance of 24-hour multichannel intraaluminal impedance-pH (MII-pH) monitoring in gastroesophageal reflux disease (GERD).Methods Fiftythree patients with GERD were enrolled in this study according to the Montreal consensus (consulting for twice reflux a week or above) from July 2011 to June 2012.Patients were divided into erosive esophagitis (EE,n =25)group and non-erosive reflux disease (NERD,n =28) group after endoscopy and MII-pH monitoring.Fifteen healthy volunteers were recruited as the normal controls.The change of pH and MII-pH parameters were compared among the three groups and the significance of the change was investigated.Results Twenty-four-hour pH monitoring showed that all the acid reflux events in the EE and the NERD groups were significantly higher than those in the control group (P ＜ 0.05).MII-pH monitoring showed that the frequencies of total reflux,acid reflux,weakly acidic reflux and percentage of acid reflux in the GERD group were higher than in the control group (total reflux:83 (54,118) vs.62 (44,111) vs.42 (20,70),P =0.003 ; acid reflux:45 (25,79) vs.22 (11,45) vs.3(1,10),P =0.000 ;weakly acidic reflux:36(18,47) vs.43(21,82) vs.23(11,43),P =0.001 ;percentage of acid reflux:53％ (37％,81％) vs.32％ (13％,48％) vs.11％ (1％,23％),P =0.002).The frequency and percentage of acid reflux in the EE group were higher than those in the NERD group (P =0.000)The percentage of weakly acidic reflux in the NERD group was higher than in the EE group (66％ (43％,79％) vs.46％ (21％,57％),P ＜0.01).The frequencies of liquid reflux and mixed reflux in the GERD groups were higher than those in the control group (22 (12,40) vs.18 (12,26) vs.9 (4,18) ; 54 (39,79) vs.42 (25,77) vs.29(14,48) ;P ＜0.01).The frequency of gas reflux in the control group was higher than in the GERD group (86(56,207) vs.31 (14,62) vs.34 (15,119),P ＜ 0.01).The frequency and percentage of proximal reflux in the GERD group were significantly higher than in the control group (28(18,41) vs.16(12,34) vs.3 (2,9) ; 33％ (22％,49％) vs.29％ (22％,35％) vs.11％ (6％,22％),P ＜ 0.001).The percentage of symptom positive index in patients with acid reflux,non-acidic reflux and total reflux in the EE group were 36.0％ (9/25),20.0％ (5/25) and 56.0％ (14/25) respectively which were higher than in the NERD group (21.4％ (6/28),14.3％ (4/28) and 35.7％ (10/28)).Conclusion MII-pH monitoring can detect more reflux events.Acid reflux plays an important role in GERD.The detective rate of GERD will be elevated when combined with MII-pH monitoring in the diagnosis.MII-pH monitoring has a distinct advantage in diagnosing GERD.

Objective: To explore the inhibitory effect of flavonoids from Sophora flavescens on the myocardial fibrosis induced by isoprenaline (Iso) and its mechanism in rats. Methods: Myocardial fibrosis model in rats was established by sc injection with Iso (5 mg/kg) for 7 d. The model rats were treated with low-, mid-, and high-dose (100, 200, and 400 mg/kg) flavonoids from S. flavescens and captopril (50 mg/kg) respectively by ig for 21 d. Myocardial indexes (heart weight/body weight, HW/BW and left ventricular weight/body weight, LVW/BW) were measured after the experiment was finished. The contents of collagen I, collagen III, tumor necrosis factor α (TNF-α) in myocardium and the level of angiotensin II (AngII) in blood serum were determined by ELISA. The concentration of malondialdehyde (MDA) in myocardium was assayed with spectrophotometry. The content of nitric oxide (NO) in myocardium was detected with colorimetry. The pathological changes of myocardium were observed. Results: Compared with the control group, the myocardial indexes and the contents of collagen I, collagen III, TNF-α, MDA in myocardium, and the level of AngII in blood serum were markedly increased, the content of NO in myocardium was decreased in the model group (P<0.05, 0.01). Compared with the model group, the myocardial indexes, the contents of collagen I, collagen III, MDA in myocardium, and the level of AngII in blood serum were markedly reduced, the content of NO in myocardium was increased in mid-and high-dose flavonoids from S. flavescens groups (P<0.05, 0.01). The level of TNF-α in myocardium was reduced in all flavonoids from S. flavescens groups (P<0.05, 0.01). HE staining showed that myocardial tissue had obviously myocardial damage and the formation of fibrosis lesions in the model group; The myocardial tissue had few fibrosis lesions in mid-and high-dose flavonoids from S. flavescens groups. Conclusion: Flavonoids from S. flavescens could inhibit myocardial fibrosis and protect myocardium in rats, and its mechanism may be associated with decreasing AngII level in circulation and myocardium, lowering TNF-α content in myocardium, and inhibiting collagen synthesis by increasing the NO level and anti-oxidation.

OBJECTIVE To study the drug-resistant diversity of Gram-negative bacilli isolated from inpatients during recent five years.METHODS A total of 1 464 Gram-negative bacilli isolated were detected and retrospectively analyzed from 1999 to 2003.Antimicrobial susceptibility testing was performed using Kirby-Bauer method.RESULTS The resistance of Pseudomonas aeruginosa to piperacillin rised from 17.6% of 1999 to 79.2% of 2003,and that to ciprofloxacin rised from 4.3% of 1999 to 36.0% of 2003.The resistance of Escherichia coli to quinolones was above 50%,while to third-generation cephalosporins was 30-40%;the resistance of E.coli to piperacillin rised from 42.9% of 1999 to 68.9% of 2003,and that to ciprofloxacin rised from 40.0% of 1999 to 73.5% of 2003.The resistance of Acinetobacter to piperacillin rised from 31.2% of 1999 to 67.5% of 2003,and that to ceftriaxone rised from 36.0% of 1999 to 74.1% of 2003.The resistance of Serratia to ceftazidime,ceftriaxone,gentamicin,amikacin and piperacillin rised sharply.Imipenem was the most active antibiotic tested against Gram-negative bacilli.Cefoperazone/sulbactam and piperacillin/tazobactam also showed excellent activity against Gram-negative bacilli.CONCLUSIONS During recent five years,the resistance of the most common Gram-negative bacilli has increased rapidly.How to delay the resistance development of common strains become a global problem.