Objective To study the changes of adriamycin-induced glomerular epithelial cells (GECs) permeability and cytoskeleton, and to explore the role and possible mechanism of epithelia growth factor (EGF) on adriamycin-induced glomerular epithelial barrier function. Methods Rat GECs on Milicell-PCF Inserts were exposed to adriamycin (0.5 ?mmol/L) 24 hours in the absence or presence of EGF. The paracelluar permeability to BSA and cell viability were evaluated. The expression of F-actin and alpha-actinin were analyzed by immunofluorescence and Leica laser scan confocal microscopy. Results After induced by adriamycin for 24 hours, paracelluar permeability to BSA and F-actin reorganization rate increased. Disassembling of cortical cytoskeleton architecture was observed. Stress fiber in cytoplasm disappeared. Alpha-actinin staining showed perinuclear enhancement, which is different from normal cytosolic pattern. EGF significantly reduced adriamycin induced effect. Higher level of BSA passed through GEC monolayer in a dose-dependent manner. EGF prevented adriamycin-induced cytoskeletal reorganization. Disassembled cortical cytoskeleton was recovered, and stress fiber appeared again. Alpha-actinin staining mainly exhibited cytosolic uniform distribution as control GEC. In addition, administration of either AG1478, a specific inhibitor of EGF-receptor tyrosine kinase, or U73122, a specific inhibitor of PLC? before EGF treatment attenuated the EGF-mediated effect, whereas neither AG1478 nor U73122 exerted influence in the absence of EGF. Conclusions Adriamycin increases GEC paracellular permeability to BSA as a result of adriamycin-induced cytoskeleton disassembling and disruption. EGF may prevent adriamycininduced cytoskeleton reorganization and maintain glomerular epithelial barrier function through EGF mediated EGF-EGFR-PLC? signal pathway.

Objective: To manage various medical consumable scientifically in different consumable materials, the new separate-store management of consumable management was developed. Methods: The medical consumable depot could be divided into the general consumable depot, the in vitro diagnostic products (IVD) depot and the high-value consumable depot, different management modes could be provided varying from different consumable depots mentioned above. Results:Different items of management processes are regulated, the full-cost accounting of consumables are promoted and the social and economic benefits of hospitals are enhanced. Conclusion: This management mode had the characteristics of normalization and practice.

Humans ; Ischemia ; metabolism ; pathology ; therapy ; Ischemic Postconditioning ; methods ; Myocardial Reperfusion Injury ; metabolism ; pathology ; therapy

The substantia gelatinosa of subnucleus caudalis of the trigeminal nerve of the rat shows an intense extralysosomal acid phosphatase activity which disappears after the removal of it's skin of the facial regions.Different regions of the facial skin of 69 rats were excised and the projections of the cutaneous nerves were traced by studying the extralysosomal acid phosphatase activity with the modified Gomori's methods.A somatotopical localization was found within the substantia gelationosa. It agreed with what has been found in earlier studies where other techniques have been used. The findings showed that the fibers of the cutaneous areas along the dorsoventral axis of the body were found in a reverse order in the receptive fields in the substantia gelatinosa. The vertex and fore-head lay in the ventral, while skin of the mandibular areas in the dorsal region, and the skin of the facial areas lay successively from ventral to dorsal in following order: vertex and forehead eye region, dorsal vibrissae, ventral vibrissae-upper lip and finally lower lip-mandibulare region, in the receptive fields of the substantia gelatinosa.The cutaneous facial regions were disproportionately represented in substantia gelatinosa, for example, the field receiving projections from the vibrissae areas or fields were as large as 4mm~2 in the most medial portion of the ventral vibrissae-upperlip which are disproportionately large while the fields receiving projections from the forehead and mandibular cutaneous regions were disproportionately small. The provides a possible explanation for the specific function of analgesia of the acupuncture point Renzhong.

Objective To observe dynamic changes of Kir2. 3 mRNA in the hippocampus of rats with chronic temporal lobe epilepsy, and to discuss the relationship between Kir2. 3 expression and the pathogenesis of chronic temporal lobe epilepsy. Methods We used pilocarpine to induce status epilepticus (SE) in rats,which became chronic temporal lobe epileptic rats in 2 weeks. The expression of Kir2.3 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) at the time points of 0, 6, 72 hours and 2 weeks after SE. Results The ratios of Kir2. 3 mRNA to β-actin of normal control and 0, 6, 72 hours, 2 weeks after SE were 0. 080 ± 0. 030, 0. 103 ± 0. 045, 0. 164 ± 0. 026, 0. 132 ± 0. 024, and 0. 011 ± 0. 008, respectively. The ratio was significantly higher 6 and 72 hours after SE and significantly lower 2 weeks after SE than that of the normal control. Conclusions Two weeks after SE, when the rats had spontaneous recurrent seizures, the expression rate of Kir2.3 reached a turning point, which possibly became the basis of epileptogenesis.

Acupuncture Therapy ; Child ; Child, Preschool ; Drugs, Chinese Herbal ; therapeutic use ; Enuresis ; drug therapy ; etiology ; Female ; Humans ; Male ; Phytotherapy

Adult ; Farmer's Lung ; Female ; Humans

The peripheral nerves supplying the acupuncture points of the facial regionwere sectioned from 63 rats and their central terminations were traced by the loss ofthe acid phosphatase(ACP)activity in the substantia gelatinosa(SG)with themodified Gomori's method.1)The supraorbital nerve was cut,the loss of the ACP was confined to thelateral aspect of the substantia gelatinosa in spinal segments C_1.2)The infraorbi-tal nerve was transected,the ACP activity was absent from the central and lateralsectors of the SG in the medulla oblongata.3)The mental nerve was divided,theACP activity was absent from the most medial sector of SG in the medulla oblon-gata and C_1.4)The auriculotemporal nerve was severed,the activity of the enzymewas absent mainly in the inner medial sector of SG in the C_1 and C_2.5)Thefacial nerve was divided,the area deprived of enzyme activity is mainly found inthe central medial sector of SG in C_2.6)The vagal nerve was divided,a medialsector or central sector of SG in the C_1 and C_2 segment is devoid of ACP.7)Thegreat auricular nerve and the cutaneous cervical nerve were severed,the ACP activitywas absent in SG of the upper four cervical cords.The above results show that the primary sensory nerve of the peripheral nerveswhich supply the facial acupuncture points terminate in the SG of the subnucleuscaudalis of the trigeminal nerves and the dorsal horn and the primary sensory nervefibre of these nerves mostly terminate in C_1 and C_2 segment.

On the model of isoprenaline (ISO)-perinjured rat heart, the protctive effect of ischemic preconditioning (IPC) on ischemia reperfusion (I/R) damage was observed.It was found that compared with alone I/R group,IPC ameliorated I/R-induced reduced reduction of coronary blood flow (CBF) (8. 8 + 0. 6 vs 6. 6 + 0. 4mL/min,P