1.Diagnosis and treatment of congenital second branchial fistula.
Jin-ke HONG ; Sheng-hong GAO ; Jun ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(12):945-946
Adolescent
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Adult
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Child
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Cutaneous Fistula
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congenital
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diagnosis
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surgery
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Female
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Humans
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Male
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Middle Aged
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Parotid Region
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abnormalities
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Young Adult
3.Mammotome system in treatment of large benign breast tumor
Hong YIN ; Fengliang WANG ; Sheng GAO ; Fei CHEN ; Cheng LU
Journal of Endocrine Surgery 2014;8(5):406-408
Objective To explore the possibility of using the mammotome system (MMT) with 2.5 cm spiral cutting mode to resect benign tumor of 3-6 cm in diameter.Methods The study group consisted of 160 patients with tumor of 3-6 cm in diameter,who received treatment of MMT cross combination with parallel rotary cutting method.The control group consisted of 160 patients with tumor < 2.5 cm and received routine MMT operation.The therapeutic effects and complications of the two groups were compared.Chi-sqare test was used for statistical analysis.Results All operations were successful on MMT.There was no statistical difference in intraoperative bleeding,skin ecchymosis and postoperative hematomas between the two groups(x2 =0.251 8,P =0.616;x2 =0.328 2,P =0.567 ;x2 =0.146 3,P =0.702).The hospitalization duration,the operation scar and other complications were similar between the two groups.Conclusions The MMT cross combination with parallel rotary cutting method can be used to resect 3-6 cm benign breast tumors.It has the advantages of safety,efficiency,minimal invasiveness,covertincision,good cosmetic effect and low complications.
4.Extrapleural solitary fibrous tumor: report of a case.
Jin-hong MEI ; Hong-ping WAN ; Han LIU ; Ren-sheng CHEN ; Hui-chun GAO ; Shan XU
Chinese Journal of Pathology 2006;35(8):510-511
5.Use of recombinant human luteinizing hormone for ovulation stimulation in in vitro fertilization-embryo transfer
Shiru XU ; Yuan LI ; Hong LIU ; Haiyan LI ; Rong TANG ; Qin GAO ; Yan SHENG ; Zijiang CHEN
Chinese Journal of Obstetrics and Gynecology 2010;45(6):420-423
Objective To evaluate application of recombinant human luteinizing hormone (r-hLH)used in ovarian stimulation of assisted reproductive technique and impact on outcome of pregnancy. Methods From Apr. To Jul. 2009, 123 patients with low LH level ( < 1 U/L) at day 3 of menstruation and downregulation of pituitary function undergoing in vitro fertilization-embryo transfer (IVF-ET) in Reproductive Medical Center, Provincial Hospital Affiliated to Shandong University were enrolled in this study, whom were classified into 66 cases treated by r-hLH in r-hLH group and 57 cases without r-hLH treatment in non-r-hLH group. In the mean time, 145 patients with normal level of serum LH ( 1-2 U/L) not given by r-hLH treatment and undergoing IVF-ET were matched as control group. Total amount of gonadotropin, estradiol levels and LH levels on the administration of human chorionic gonadotropin ( hCG), number of oocytes retrieved, number of 2PN zygotes, rate of high quality embryos, the rates of implantation and clinical pregnancy were compared among these three groups. Results The level of serum LH on the day of hCG administration were ( 1.59 ± 0.77 ) U/L in r-hLH group, (0.54 ± 0.25 ) U/L in non-r-hLH group and (2.39 ± 1.01 ) U/L in control group, which reached tatistical difference between every two groups (P < 0.05). The rates of high quality embryo were 59.36% in r-hLH group, 57.79% in non-r-hLH group,which were significantly lower than 65.94% in control group, respectively (P < 0. 05 ). The rates of 2PN were 67.62% in r-hLH group and 68. 32% in control group, which were significantly higher than 62. 84% in non-r-hLH group, respectively ( P < 0.05 ). The rates of implantation of 29.77% in r-hLH group were significantly higher than 18.26% in non-r-hLH group ( P < 0.05 ). The total amount of gonadotropin,estradiol level on the day of hCG administration, the number of oocytes retrieved, and clinical pregnancy rate were not significantly different among those three groups ( P > 0.05 ). Conclusion The administration of recombinant human uteinizing hormone in patients who are profoundly suppressed after down-regulation with long protocol can get more quality embryos, the higher rates of 2PN and implantation.
6.Enhancing the accumulation of beta-amyrin in Saccharomyces cerevisiae by co-expression of Glycyrrhiza uralensis squalene synthase 1 and beta-amyrin synthase genes.
Ying LIU ; Hong-Hao CHEN ; Hao WEN ; Ya GAO ; Li-Qiang WANG ; Chun-Sheng LIU
Acta Pharmaceutica Sinica 2014;49(5):734-741
Glycyrrhiza uralensis Fisch. ex DC is widely used in traditional Chinese medicine (TCM). Among its various active components, glycyrrhizic acid is believed to be the marker component. Squalene synthase (SQS) and beta-amyrin synthase (beta-AS) are key enzymes in the biosynthetic pathway of glycyrrhizic acid in G uralensis. To reveal the effects of co-expression of SQS1 and beta-AS genes on this pathway, 7 yeast expression vectors harboring different SQS1 variants and beta-AS were constructed and expressed in Saccharomyces cerevisiae as fusion proteins. TLC and GC-MS results showed that co-expression of SQS1 and beta-AS enhanced the accumulation of beta-amyrin. The effects of SQS12 were more obvious than the other two SQS1 variants. This study is significant for further investigations concerned with exploring the biosynthesis of glycyrrhizic acid in vitro and strengthening the efficacy of G. uralensis by means of increasing the content of glycyrrhizic acid.
Farnesyl-Diphosphate Farnesyltransferase
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genetics
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metabolism
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Glycyrrhiza uralensis
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genetics
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Intramolecular Transferases
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metabolism
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Oleanolic Acid
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analogs & derivatives
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metabolism
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Plant Proteins
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genetics
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Recombinant Proteins
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metabolism
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Saccharomyces cerevisiae
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metabolism
7.Analysis of therapeutic outcomes of polycystic ovary syndrome patients with hyperprolactinemia
Yuhua SHI ; Yan SHENG ; Zijiang CHEN ; Junhao YAN ; Qin GAO ; Hong LIU ; Zengxiang MA
Chinese Journal of Obstetrics and Gynecology 2008;43(4):251-253
Objective To study effect of drug treatment in polycystic ovary syndrome patients withhyperprolactinemia.Methods We retrospectively studied 63 women with polycystic ovary syndrome and hyperprolactinemia from the Reproductive Medicine Center,Provincial Hospital between January 2005 andMarch 2007.According to the beginning time of bromocriptine,all women were divided into two groups.Group Ⅰ was composed of 48 cases who received bromocriptine administration before induction of ovulation cycles,and the dose of bromocriptine was modulated depending on the level of serum prolactin.When serum prolactin was controlled at normal levels,we decreased the dosage of bromocriptine step by step(1.25 mgonce),and then continued the treatment at maintenance dosage for no less than 3 weeks.After a baselineultrasonographic examination on day 3,patients were treated with clomiphene citrate at a dosage of 100 mg (2 tablets/day)for 5 days of a normal cycle or progesterone-induced bleeding.On day 9,we monitored the growth conditions of follicles routinely with trans-vaginal uhrasound.If there was no dominant follicle,we added human menopausal hormone(hMG,75 U/d)to the protocol.Human chorionic gonadotropin(hCG,6000-10000 IU)was given intramuscularly when the mean diameter of a follicle reached at least 18 mm.At the same time we instructed the patients to have sexual intercourses or carried out artificial inseminationsbefore and after ovulation.Group Ⅱ were 15 cases in which induction of ovulations were commenced almostsimultaneously with beginning of bromocriptine.The same protocol was given to patients in group Ⅱ.The procedures of ovulation induction and the outcomes of treatment were analyzed and compared.Results Compared with groupⅡ.the days of using hMG in Group Ⅰ was shorter by instructing the time of sexualintercourse.The difference was significant(P=0.004).And there were similar results in the artificial insemination cycles(P=0.009).The rate of pregnancy in group Ⅰ(40%,19/48)was higher than that in groupⅡ(27%,4/15),but the difference was not obvious(P=0.525).Conclusion Bromocriptine administration before the stimulated ovulation therapy can decrease the total dosage and treatment course of ovulating drugs.Induction of ovulations simultaneously with start of bromocriptine therapy can shorten the treatment time of infertility.
8.Diagnosis and treatment of early postoperative infiammatary ileus
Gang AN ; Sheng ZHU ; Hong WANG ; Zhihui SUI ; Taotao GAO ; Chuanlong MIAO
Chinese Journal of Primary Medicine and Pharmacy 2009;16(7):1200-1201
Objective To explore the principle of diagnosis and treatment of early postoperative inflammatory ileus. Method To retrospectively analyse the clinical data and treatment results of early postoperative inflammatory ileus. Results 15 cases after fasting, decompression, nutritional support, somatostatin and dexamethasone, and other conservative treatment were cured. Conclusion Early postoperative inflammatory ileus usually occurred in 2 weeks, has a typical performance of the obstruction, conservative treatment is effective.
9.Application of paclitaxel during trabeculectomy and its anti-proliferative effects
Jing, CHI ; Xiao-hua, MA ; Hong-sheng, BI ; Jing, GAO ; Zhong-en, LI
Chinese Journal of Experimental Ophthalmology 2011;29(1):43-47
Background The filtering surgery is the main method of treating glaucoma.Fibrosis of filtering bleb is a key cause of failure of operation.The study about application of anti-scaring drug in filtering surgery is a hotspot.Objective Present study was to investigate the anti-scaring effect of paclitaxel aher trabeculectomy.Methods Thirty-two adult clean domestic rabbits underwent standardized trabeculectomy and randomly divided into 4 groups.Normal saline solution was used beneath the scleral flap during trabeculectomy for 3 minutes in 16 eyes of 8 rabbits as controls.0.3 g/L mitomycin C,0.2 g/L paclitaxel or 0.3 g/L paclitaxel was administered at the same way respectively in other 3 groups.Intraocular pressure (IOP) was measured,and eye numbers with function blebs were compared among 4 groups at the 4th,7th,14th and 28th day after surgery.The opening of filtration tunnel and the number of inflammatory cells were observed by hematoxylin and eosin staining,and proliferation of new collagen fibers was evaluated by Masson trichrome method.This study complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission(version 1988). Results No significant differences were found in the change of lOP among 4 groups before operation(F=0.54,P=0.83)and the 4th day aher operation(F=0.57,P=0.87).The IOP value was statistically lower in 0.3 g/L mitomycin C group,0.2 g/L paclitaxel group and 0.3 s/L paelitaxel group than the normal saline solution group(P<0.05)with the lowest value in 0.3 s/L paclitaxel group in 7,14,28 days after operation(P<0.05).Functional filtering bleb was seen in all the rabbit eyes in the 4th day after operation.In 7,14,28 days after operation,the number of eyes with functional bleb wag evidently more in 0.3 g/L mitomycin C group,0.2 g/L paclitaxel group and 0.3 g/L paclitaxel group compared with normal saline group(P<0.05).The histological examination showed that the infiltration of inflammatory cells in filtering tunnel was much more obvious in normal saline solution group than the other groups with the most mild response in 0.3 g/L paclitaxel group.Masson trichrome revealed that proliferation of new collagen fibers in 0.3 g/L paclitaxel group was significantly decreased in comparison with those in other three groups at the 4th,7th,14th and 28th day after surgery(all P<0.05). Conclusion Paclitaxel can inhibit the inflammatory response and collagen fibrosis and therefore open the filtering tunnel after it be used topically during the glaucoma trabeculectomy.
10.Study on SNP polymorphism of mitochondrial DNA D-loop region from Nu ethnic population in Yunnan of China.
Shu-hui GAO ; Qing-bo LIU ; Hong-sheng GUI ; Ke QIAO ; Sheng-bin LI
Chinese Journal of Medical Genetics 2007;24(3):354-357
OBJECTIVETo investigate the mitochondrial single nucleotide polymorphism (SNP)of Chinese Nu ethnic population from Yunnan region of China and to provide basic database for ethnic origin investigation and forensic purpose.
METHODSGenomic DNA from the whole blood of 87 unrelated individuals was extracted by standard chelex-100. The sequence polymorphism was analyzed by PCR-based assay and using ABI 3730 Analyzer to detect many number of relatively common point mutations.
RESULTSSixty-two SNP loci were observed among them with 492 point mutations and 59 haploids identified in mitochondrial DNA hypervariable region I (mtDNA HVSI). The gene diversity was estimated to be 0.9675,and the random match probability was calculated to be 0.0437.
CONCLUSIONThe result suggests that mtDNA HVSISNP database of Nu ethnic population can be a useful tool for forensic identity and original research.
Asian Continental Ancestry Group ; genetics ; Base Sequence ; China ; DNA, Mitochondrial ; genetics ; Databases, Genetic ; Ethnic Groups ; genetics ; Forensic Genetics ; Humans ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA