Objective To explore the effect of cognitive behavior intervention on the defense style of patients with depression. Methods One hundred and forty-six patients with depression in hospitalized were divided into study group and control group according to the set sequence. The study group and the control group were given cognitive behavioral intervention and routine nursing, respectively. All patients were assessed by Hamilton depression scale (HAMD), Hamilton anxiety scale (HAMA), Simplified Coping Style Questionnaire (SCSQ), and Defense Style Questionnaire (DSQ) . Results The differences of conceal type and positive coping factor were significantly before and after intervention in the study group ( < 0.05) . In the 6th week, the differences of conceal type and positive coping factor were significantly between the two groups ( < 0.05) . The differences of HAMD and HAMA scores were significantly between the 0 th and 6 th weeks of the two groups ( <0.05) . There was negatively correlated between HAMD and positive coping factor ( <0.05) . There were positively correlated between HAMD and the cover up defense mode ( <0.05),HAMA and the cover up defense mode ( <0.05) . Conclusion The cognitive behavior intervention could improve the depression and anxiety symptoms and defense style in patients with depression.

Objective To study the influence of cognitive behavioral intervention on the quality of marriage and family function in patients with depression. Methods 146 hospitalized patients with depression were divided into study group and control group according to the set sequence. The study group were given cognitive behavioral intervention and the control group were given routine nursing.All patients were assessed by Hamilton depression scale (HAMD), Hamilton anxiety scale (HAMA), family functioning (FAD), marriage quality questionnaire (ENRICH) to evaluate the depression and family function. Results The difference in ENRICH total score, marital satisfaction, conflict resolution, children and marriage, leisure activities and the relationship with family and friends,FAD total score, problem solving, emotional response was statistically significant between before and after the intervention of the study group ( <0.05) . In the 6th weeks, the difference in ENRICH total score, marital satisfaction, conflict resolution methods, children and marriage,leisure activities and the relationship with family and friends, FAD total score, problem solving,emotional response was statistically significant between the two groups ( <0.05);The difference in HAMD,HAMA score was statistically significant between the 0th and 6 th weeks of the two groups ( <0.05) . There was a negative correlation between HAMA and conflict resolution factor (=-0.48, <0.05) . There was a positive correlation between FAD and the HAMD total score (=0.43,<0.05) . There was a negative correlation between ENRICH and HAMD total scores (r=-0.46, <0.05) .Conclusion The cognitive behavioral intervention can improve the family function and the quality of marriage in patients with depression.

ObjectiveTo study the effect of platelet aggregation rate (PAR) level on the prognosis of percutaneous transluminal coronary angioplasty (PCI).MethodsFour hundred and one patients who performed PCI were divided into 4 groups:group 1 (PAR≤30％,103 cases),group 2 (PAR 31％ - 40％,102 cases),group 3 (PAR 41％ - 49％,103 cases ) and group 4 (PAR ＞ 49％,93 cases ).All the patients were followed up.The survival analysis was performed in 4 groups.ResultsThe data of survival table were tested by 3 methods among 4 groups:statistic was 0.612,degree of freedom was 3,P =0.894 in Log-rank (Mantel-Cox) test,statistic was 0.567,degree 9f freedom was 3,P =0.904 in Wilcoxon test and statistic was 0.586,P =0.900 in Tarone-Ware test.There was no significant difference among them.The mean follow-up duration was 2.2 years,and there was no significant difference in survival function among 4 groups (P ＞0.05).ConclusionPreoperative and postoperative measurement of PAR levels may not improve the prognosis.

Objective To investigate the meaning of expression of apoptosis related molecules NFKBp65 and p53 and GPX1-mRNA in patients with Keshan disease(KSD).Methods Sixteen chronic Keshan Disease patients were enrolled in KSD group according to electrocardiogram,chest X ray film and clinical examinations on 15,September in 2009,and 23 healthy people were included in control group from physical examination taken in The Second Affiliated Hospital of Xi'an Jiaotong University.Fresh blood(5 ml)was collected from antecubital vein of all subjects in the fasting state.Total mRNA and protein of blood sample were isolated using Trizol.GPX Assay Kit was used to detect GPX enzyme activity,and GPX1-mRNA expression was determined by SYBR Real-Time PCR.Meanwhile,expression of apoptosis related molecules NFKBp65 and p53 were determined by Western blot.Results GPX enzyme activity decreased significantly in KSD group[(108.61±14.10)U]compared with control group[(122.78±11.89)U,t=2.874,P<0.05],GPX1-mRNA level of KSD group(0.553±0.299)notably KSD group(0.802±0.057)compared with control group[(1.065±0.355),t=6.829,P<0.01].p53 increased in KSD group(1.604±0.191)compared with control group[(1.137±0.186),t=3.033,P<0.05].Conclusiom Decreased GPX1-mRNA expression may result in lower GPX enzyme activity of patients with KSD.Thus oxidative damage increases and cadioeyte apoptosis is activated by activating apoptosis signal pathway.

Actins ; metabolism ; Bone Neoplasms ; metabolism ; pathology ; surgery ; Desmin ; metabolism ; Diagnosis, Differential ; Fibrosarcoma ; metabolism ; pathology ; Humans ; Leiomyosarcoma ; metabolism ; pathology ; surgery ; Male ; Middle Aged ; Neurilemmoma ; metabolism ; pathology ; Tibia ; Vimentin ; metabolism

The aim of this study was to design a simple, economic, with high Common Mode Rejection Ratio (CMRR), preamplifier and multi-channel masticatory muscle surface electromyography (sEMG) signal acquisition system assisting to diagnose temporomandibular disorders (TMD). We used the USB interface technology in the EMG data with the aid of the windows to operate system and graphical interface. Eight patients with TMD and eight controls were analyzed separately using this system. In this system, we analyzed sEMG by an optional combination of time domain, frequency domain, time-frequency, several spectral analysis, wavelets and other special algorithms under multi-parameter. Multi-channel sEMG System of Masticatory Muscles is a simple, economic system. It has high sensitivity and specificity. The sEMG signals were changed in patients with TMD. The system would pave the way for diagnosis TMD and help us to assess the treatment effect. A novel and objective method is provided for diagnosis and treatment of oral-maxillofacial disease and functional reconstruction.
Algorithms ; Computer Graphics ; Data Collection ; Electromyography ; Humans ; Masticatory Muscles ; physiology ; physiopathology ; Sensitivity and Specificity ; Signal Processing, Computer-Assisted ; Temporomandibular Joint Disorders ; diagnosis ; User-Computer Interface

Objective To analyze the expression characteristics of SRPK2 ( serine /arginine-rich protein specific kinase 2 mRNA and its encoded protein products in mouse testis, and to reveal its molecular role during spermatogenesis. Methods Using semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting to an-alyze the expression of SRPK2 mRNA and its protein products in several mouse tissues.The stage-specific expression pat-tern of SRPK2 mRNA in mouse testis was examined by real-time quantitative PCR.Immunohistochemical staining was ap-plied to identify the SRPK2 protein distribution in mouse testicular seminiferous tubules and indirect immunofluorescence staining was used to detect the subcellular localization of SRPK2 protein in spermatic cells.Results Semi-quantitative RT-PCR and Western blotting analysis revealed that SRPK2 was highly expressed in mice testis.Real-time quantitative PCR analysis showed that SRPK2 mRNA was expressed abundantly at the stage of 5-and 8-week old mouse testes, suggesting that SRPK2 gene has stage-specific expression patterns during mouse spermatogenesis.Immunohistochemical and indirect immu-nofluorescence staining demonstrated that SRPK2 protein was located around the surface of elongated sperm nucleus. Conclusions SRPK2 is highly expressed in mouse testis and has significant stage-specific expression characteristics with distinct nuclear localization.These results indicate that SRPK2 may participate in precursor mRNA splicing during mouse spermiogenesis.The molecular mechanism of SRPK2 is yet to be further studied.

Objective To explore the role of stromal interaction molecule 1(STIMI)on prohteration and intra- cellular Ca~(2+)change in vascular smooth muscle ceils(VSMC).Methods Rat VSMC were isolated from SD rats and primary cultured.Ad-si/rSTIM1 and Ad-hSTIM1 were transfected into VSMC.The protein of STIM1 was measured by Western blot,the proliferation of VSMC was analyzed by ~3H-thymidine(~3H-TdR)incorporation and cell count,the intracellular Ca~(2+)change was assessed By Aquaeosmos system.Ruselts Fourty-eight hours after transfection,as compared with Ad-hSTIMI group,the Ad-si/rSTIMI VSMC had lower expression of STIM1 protein (P

BACKGROUND: Common complication of traditional joint replacement is hip joint dislocation and large head joint implant can effectively solve this problem. OBJECTIVE: To investigate the preliminary efficacy of large head metal-on-metal implants for patients undergoing total hip arthroplasty. DESIGN, TIME AND SETTING: Prospective cohort study. The patients were selected from Department of Joint Surgery, Xinjiang Hospital of Traditional Chinese Medicine between February 2007 and January 2008. PARTICIPANTS: A total of 65 patients (71 hips) undergoing large head metal-on-metal implantation in Xinjiang Hospital of Traditional Chinese Medicine were selected, including 21 females and 44 males with an average age of 54.6 years (ranging 19 to 77 years). Of them, there were 28 cases of necrosis of the femoral head, 24 of hip osteoarthritis, 9 of femoral neck fracture and 4 of acetabular dysplasia. METHODS: According to age, sex, cause of disease and preoperative joint function, the patients were divided into traditional prosthesis (n=30, 33 hips, metal-on-polyethylene implant) and large head M-O-M group (n=35, 38 hips, ASR/XL prosthesis, DePuy, Motech, Warsaw, IN, USA). MAIN OUTCOME MEASURES: The pre and postoperative Harris scores for the hip, range of motion, periprosthetic radiolucency, leg-length inequality and complications were recorded and compared. RESULTS: All patients were followed-up. The traditional group was followed up for 20 months (ranging 13-28 months), and the large head M-O-M group was followed up for 17 months (ranging 10-26 months). The mean Harris score was 89 points (ranging 59-98) in traditional group, and 94 points (ranging 71-100) in large head M-O-M group (P=0.014). The range of motion of hip was improved, and the large head M-O-M group (34?) was superior to traditional group (26?, P=0.004) at 6 months after surgery; the range of motion was improved 27? in traditional group and 37? in large head M-O-M group (P=0.009) over 1 year of follow up. The leg-length inequality was 4 mm (2-11 mm) in large head M-O-M group and 7 mm (5-16 mm) in traditional group (P=0.005). Both groups exhibited periprosthetic radiolucency (≤1 mm, 2 cases in traditional group and 3 cases in large head M-O-M group). One patient in the traditional group suffered dislocation. CONCLUSION: The large head M-on-M implants in total hip arthroplasty has excellent short-term effects on patients compared with traditional prosthesis. They offer the more stability and better restoration of hip articulation function and biomechanical reconstruction.

OBJECTIVETo investigate the effect and mechanism of Gualou Xiebai (GLXB) decoction on pulmonary fibrosis.

METHODPulmonary fibrosis was induced by bleomycin A5 in SD rats. Rats in treatment group were killed after being treated by GLXB by decoction daily for 28 days. The lungs of all rats were harvested for histopathological studies. The contents of platelet-derived growth factor BB (PDGF-BB) in lungs were measured and compared.

RESULTThe contents of PDGF-BB in lungs have increased significantly in model group compared with normal group and treatment group (P<0.05).

CONCLUSIONGLXB decoction could significantly alleviate the increased PDGF-BB expression in lungs of pulmonary fibrosis rats.

Animals ; Bleomycin ; analogs & derivatives ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Female ; Gene Expression ; drug effects ; Immunohistochemistry ; Lung ; drug effects ; metabolism ; Platelet-Derived Growth Factor ; metabolism ; Proto-Oncogene Proteins c-sis ; Pulmonary Fibrosis ; chemically induced ; drug therapy ; metabolism ; Rats