1.Inflammatory stress induces insulin resistance in CD36 knockout mice
Xian CHEN ; Qing LIU ; Han LEI ; Hong LIU
Journal of Third Military Medical University 2003;0(09):-
Objective To explore whether the inflammatory stress can increase insulin resistance in the CD36 knockout (KO) mice.Methods After the mice were fed a standard chow for 14 weeks,oral glucose tolerance test,insulin release tests,lipids metabolism,SAA,IL-6,TNF-? and hepatic mRNA and proteins expression of mTOR,S6K,IRS-1,pIRS-1,2 were measured.Results Compared with the wide-type,the CD36 KO mice un-inflamed exhibited insulin resistance,and the insulin resistance index was increased[(3.01?1.24) vs (0.81?0.12),P
2.Extraction and purification of giant salamander skin mucous glycoprotein and study its anti-cancer activity of lung cancer
Weiliang XU ; Dejing CHEN ; Yu LIU ; Hong WEI ; Qing LIU
Chinese Journal of Biochemical Pharmaceutics 2015;(8):44-47
Objective To study the appearance of skin mucous glycoprotein in vitro on the activity of human lung cancer cells A549. Methods Used alkali extraction and DEAE-52 ion exchange chromatography and Sephadex G-100 gel chromatography purification salamander mucous glycoprotein; Salamander mucous glycoprotein inhibition of human lung cancer cells A549 was detected by MTT colorimetric method in vitro.ResuIts It showed that the total sugar content in the appearance of mucus was 4.23%, the relatively pure glycoprotein component, by SDS protein electrophoresis tests, it contained a single glycoprotein component, its molecular weight was about 30 kDa.With glycoprotein pure concentration increased from 1,10, 20,40μg/mL, the glycoprotein inhibition rate of A549 cells increased; when the glycoprotein concentration was 40 μg/mL, for 24 h action, the inhibition rate of A549 cells was up to 85.66 %, while the role of 48 h, the inhibition rate of A549 cells was up to 92.32%.Inhibition effect of mucus glycoprotein on A549 cell compared with positive control drug, had significant difference.ConcIusion Salamander mucus glycoproteins of human lung cancer cells has obvious inhibitory effect, can provide theoretical basis for the development of lung cancer drug resistance.
4.Case of up-rushing gas syndrome and chest apoplexy.
Hong-Wei JI ; Qing-Zhang FENG ; Fang-Ming LIU
Chinese Acupuncture & Moxibustion 2013;33(10):904-904
Acupuncture Therapy
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Chest Pain
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therapy
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Female
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Humans
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Middle Aged
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Stroke
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therapy
5.A clinical analysis of dermatomyositis with interstitial lung disease in 20 patients
Hong LI ; Qing LIN ; Hongmei JIAO ; Xinmin LIU
Chinese Journal of Internal Medicine 2012;51(3):214-216
Objective To investigate the clinical features of the patients with dermatomyositis (DM)complicated with interstitial lung disease(ILD).Methods The clinical manifestations and the laboratory data of the 20 patients with DM complicated with ILD were retrospectively analyzed.Results Among 169 DM patients,20(11.8%)were founded with ILD and 4 had no complaint of respiratory system.The ratio of male to female was 1∶2.33.The level of serum creatine kinase(CK)was elevated in 11 cases.Anti-Jo-1 antibody was positive in 4 cases.Bilateral infiltrates including ground-glass attenuation,interlobular septal thickening and reticular opacities were found by chest high resolution computed tomography.The impairment of lung function was manifested as the decreased diffusion capacity for carbon monoxide of the lung(DLCO)and restricted ventilatory function abnormalities.Fifteen cases showed hypoxemia or respiratory failure by arterial blood gas analysis.All patients were treated with glucocorticoid and/or immunosuppressive agents.Totally,14 patients had improved,while 2 patients with no improvement and 4 patients died with a mortality of 20%.Conclusions DM complicated with ILD which is easily missed has a high morbidity and mortality.The understanding of DM complicated with ILD should be enhanced,thus early diagnosis and intervention could be performed for better prognosis.
6.The building of the county level hospital nursing assistance need indicator system in Jiangxi province
Congxiang LIU ; Langjuan TANG ; Qing LI ; Yan HONG
Chinese Journal of Practical Nursing 2014;30(22):7-11
Objective To build the county level hospital nursing assistance need indicator system in Jiangxi province.Methods Literature review,qualitative research method and Delphi expert consultation method were adopted to build the county level hospital nursing assistance need indicator system.The indicator system was revised through trial test.Results Two rounds of expert consultation showed that expert authority degree coefficients were 0.79,0.83; Coordination coefficient(Kendall's W) was 0.11~0.16; content experts consistency coefficient was 0.78,reliability Cronbach's α coefficient was 0.85.Through expert advice and pre-examinaion,the county level hospital nursing assistance need indicator system was built,consisting of 3 level Ⅰ indicators,8 level Ⅱ indicators,43 level ⅠⅡ indicators,including assistance contents,assistance ways and assistance time.Conclusions This study established the county level hospital nursing assistance need indicator system with better scientific and feasible characteristics.It can provide experience and guidance for the relevance and effectiveness of the implementation of follow-up care aid work.
7.Intravascular lymphomatosis of the prostate gland.
Xiu-rong YIN ; Hong LIU ; Hui-qing CHEN
Chinese Journal of Pathology 2005;34(3):189-190
Aged
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Antigens, CD20
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metabolism
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Humans
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Leukocyte Common Antigens
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metabolism
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Lymphoma, B-Cell
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complications
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metabolism
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pathology
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surgery
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Male
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Prostate
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pathology
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Prostatectomy
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Prostatic Hyperplasia
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complications
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metabolism
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pathology
;
surgery
8.Clinical Study of Advanced Primary Hepatocellular Carcinoma Cells After TACE with use of Portal Vein Perfusion Chemothepary
Yamin LIU ; Dongmei JIANG ; Hong QING ; Xiaohong FANG ; Qingyong MA
Journal of Practical Radiology 1992;0(11):-
Objective To study the efficacy of transcatheter arterial chemoembolization (TACE )and continuous portal vein perfusion chemothepary (VPC) on advanced hepatocellular carcinoma cells (HCC). Methods The patients in group A (n=83) received the epirubicin (EPI) 40 mg and the cisplatin (CDDP) 80mg, lipiodol-anticancer drug emulsion was injected into the feeding arteries of tumors followed by embolization with gelatin sponge (GS) particles. Patients in group B (n=59) after receiving the same as the group A then VPC was carried out. Results The response rate ( CR + PR) in group A and B was 45.8% and 61.0%(P
9.Analysis of Trace Elements in Blood of 312 Children with Rachitis in Qingdao
qing-yi, ZHU ; jing-dong, LIU ; yu-hong, JIANG
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To investigate the relationship between trace elements and rachitis in children.Methods Three hundred and twelve patients with rachitis and 297 healthy children were selected for this study.Blood zinc(Zn),iron(Fe),plasma copper(Cu),calcium(Ca),magnesium(Mg),lead(Pb) and cadmium(Cd) were assayed by atomic absorption spectrophotometer.Results The levels of Zn,Fe,Cu of rachitis in blood were significantly lower than those of healthy children,while the levels of Mg,Pb were higher.There were significant differences between 2 groups(P
10.A case of intestinal neuronal dysplasia (type B).
Shan-xian LOU ; Hong-qi SHI ; Qing-wei LIU
Chinese Journal of Pathology 2005;34(9):611-612
Autonomic Nervous System Diseases
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metabolism
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pathology
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surgery
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Colon
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Enteric Nervous System
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abnormalities
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pathology
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Hirschsprung Disease
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pathology
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Humans
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Infant, Newborn
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Intestinal Diseases
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metabolism
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pathology
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surgery
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Male
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Phosphopyruvate Hydratase
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metabolism
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S100 Proteins
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metabolism