Objective To study the expression of survivin in malignant pleomorphic adenoma,relay pleomorphic adenoma and pleomorphic adenoma.Methods The transcription and interpretation of urvivin mRNA in the salivary gland tumor were analyzed via immunohistochemistry and in situ hybridization methods.Results Survivin mRNA expression and protein expression were almost the same in malignant pleomorphic adenoma,relay pleomorphic adenoma and pleomorphic adenoma.But they were different in the gland around the malignant pleomorphic adenoma.There was a significant difference of Survivin mRNA and protein expression in pleomorphic adenoma and malignant pleomorphic adenoma,and relay pleomorphic adenoma.The expressions were almost the same in relay and malignant pleomorphic adenoma.Conclusion The expression of survivin mRNA and protein is increased in pleomorphic adenoma and may be involved in the onset and progression of pleomorphic adenoma.It indicates that mRNA is relatively stable in the mRNA transcription and protein interpretation in malignant pleomorphic adenoma,and it is also showed earlier in gene amplification than in protein expression of the malignant tumor in the initial stage of malignant pleomorphic adenoma.Survivin can be a new diagnostic/therapeutic target in pleomorphic adenoma.

Objective To evaluate the clinical effect and safety of homemade biodegradable drug eluting stents(Excel stents)in patients with coronary heart disease.Methods Two hundred and twelve patients who received Excel stents implantation during February,2006 to March 2007 were included in the study.Procedural success rate and complications during hospitalization were monitored and all the patients were followed up clinically up to 8-21 months after discharge.Results The procedural success rate was 99.1%(210/212).A total of 420 Excel stents were implanted while direct stenting was applied in 125 patients(29.8%).Sudden cardiac death happened in 1 patient during hospitalization.Two hundred and six patients finished their clinical follow up and among them,6 patients had recurrant angina.Fifty patients received CAG examination 6-9 months after PCI and 2 patients reached the criteria of restenosis(stenosis ≥50%)and required revascularization.Conclusion Homemade biodegradable drug eluting stents(Excel stents)are safe and effective for PCI in coronary heart disease patients.

Tumor growth depends on the tumor microenvironment(TME).Tumor-associated neutrophils(TANs)are important inflammatory cells in TME.TANs are divided intoN1type with anti-tumor effect andN2type of tumor-promoting effect.Therefore,TANs have both beneficial and harmful aspects of the body.A large number of studies have been shown that TANs affect tumor formation,metastasis,angiogenesis and immune response,regulated by the secretion of cytokines and chemokines.This review will summarize the biological characteristics of TANs,and tumor development,prognosis and treatment of tumor as well as research progress of the relationship between TANs and tumor.

Objective To analyze the effect of conductive education combined with massage on activity of children with severe cerebral palsy. Methods 11 children (7 males and 4 females) with cerebral palsy receiving rehabilitation therapies in CereCare Wellness Centre of Xuhui, Shanghai from April to October 2010 were enrolled in this study. The type of cerebral palsy was as follows: 7 tetraplegia, 1 diplegia,2 athetoid, and 1 dystonia. The average age was 7.47 years, ranging from 4.7～11.9 years. The participants were divided randomly into 2groups. 5 children in group A accepted conductive education and massage therapy in the first 3 months, and then accepted routine rehabilitation therapies in the next 3 months. 6 children in group B accepted routine rehabilitation therapies in the first 3 months, and then accepted conductive education and massage therapy in the next 3 months. The phase of conductive education combined with massage was called intervention period, and the phase of routine rehabilitation therapies was called contrast period. The gross motor function was assessed with Gross Motor Function Measure Scale (GMFM), while the fine motor function was assessed with Fine Motor Function Measure Scale (FMFM).Pediatric Evaluation of Disability Inventory (PEDI) was used to assess activities of daily living. The different effects between conductive education combined with massage and routine rehabilitation therapies were compared. Results The scores of GMFM and FMFM both increased in intervention period, while both decreased in contrast period. The score of FMFM increased significantly (P<0.05). The scores of PEDI changed without statistical significance. Conclusion Conductive education combined with massage may increase the fine motor function of children with severe cerebral palsy compared with routine rehabilitation therapies.

Objective To study the biological exposure limit in bone metabolism damage caused by coexposure to fluorine and arsenic from coal burning in exposed population.Methods One hundred and ninety-eight cases of fluoride and arsenic co-exposed people from Liuchang village,Qinzhen city,Guizhou province were enrolled in the study.Urinary fluorine (UF),urinary arsenic (UAs),urinary hydroxyproline (UHYP),ross-linked Ntelopeptides of type Ⅰ collagen(UNTX) and bone strength index(STI) were detected.BMDS Version 2.1 software was used to calculate UF,UAs benchmark dose (BMD) and its lower confidence limit (BMDL) on the damage of bone metabolism caused by co-exposure to fluorine and arsenic from coal burning.Results The BMD and BMDL range of UF caused by co-exposure to fluorine and arsenic from coal burning were 0.68-1.35 mg/g Cr,0.57-1.11 mg/g Cr.The BMD and BMDL range of UAs caused by co-exposure to fluorine and arsenic from coal burning were 8.36-18.77 μg/g Cr,7.12-15.40 μg/g Cr.Conclusion The biological exposure limits of UF and UAs for bone metabolism toxicity are proposed as 0.57 mg/g Cr and 7.12 μg/g Cr in co-exposure to fluoride and arsenic from coal burning,respectively.

Cisplatin is effective in the treatment of various solid tumors. However, its clinical application is limited because of the severe side-effects such as nephrotoxicity, hepatotoxicity, ototoxicity and so on. Drug combination is advocated in clinics for synergism and detoxication. In order to provide some referencs for the research of drug combination and clinical treatment regimen, the research advances in the synergism and detoxication of drug combination of cisplatin with chemicals/ traditional Chinese medicines in recent years are reviewed in the paper.

Objective To perform the clinical characferistics of diagnosis and treatment of duodenal trauma and to improve the result of surgical treatment in patients with duodenal injury.Methods The clinical data of 32 cases with duodenal injury were analyzed retrospectively.Results All cases were surgically treated.26 cases were cured 13 cases were with complications,of which 9 cases with duodenal fistula,4 cases with peritoneal infection.6 cases died.Conclusion Early diagnosis and surgical treatment,proper management and intensive postoperative care can improve the result of patients with duodenal trauma and decrease complications.

The tumor microenvironment plays a vital role in the process of tumorigenesis and development.Studies have been confirmed that the tumor microenvironment heterogeneity has a huge impact on the tumor efficacy and drug resistance.This review summarizes the relationship between immune cells and related immune factors in tumor microenvironment as well as vascular endothelial cell heterogeneity and tumor progression and prognosis.So it is better help us understand the tumor microenvironment heterogeneity for the impact of the tumor.This will conduce to us through a variety of methods to enhance the body's anti-tunor ability by inhibi-ting and killing tumor cells.

Based on the analysis of current condition of local medical schools and medi-cal talents demand at the grassroots level,this paper indicates that,taking students’personality development as a breakthrough point,with practice platform construction as main part,supple-mented by multi-knowledge hierarchy and scientific and cultural exposure,backed by workable policy.

OBJECTIVETo analyze the effect of Chinese herbs used by Prof. LI Tao on peripheral blood T subsets in treating multiple sclerosis (MS) by using association rules and statistical methods, thereby providing evidence for optimizing prescriptions.

METHODSData of MS inpatients and outpatients recorded by data collecting system, Xiyuan Hospital, China Academy of Chinese Medical Sciences were resorted. The relationship between Chinese herbs and T cell subsets were analyzed using SPSS17.0 and Aprior module in SPSS Clementine 12.0.

RESULTSRadix bupleuri, Radix Paeoniae alba, Fructus Aurantii, Atractylodes, and Radix Glycyrrhizae were most commonly used herbal combinations.Radix Aconiti lateralis preparata and Rhizoma Smilacis glabrae were often added. Radix Aconiti lateralis preparata was associated with decreased Th1 cells (confidence level 83.78%, supportive level 36.26%). Decreased Th1 cell was associated with Radix Aconiti lateralis preparata (confidence level 71.26%, supportive level 36.26%).Radix Aconiti lateralis preparata was obviously associated with decreased Th1 cells. Radix Bupleuri, Radix Paeoniae alba, bitter orange, Atractylodes , Radix glycyrrhizae, and Radix Aconiti lateralis preparata could reduce peripheral blood Th1 subsets of MS patients and elevate Th2 subsets (all P < 0.01).

CONCLUSIONSThe herbal combination of Radix Bupleuri, Radix Paeoniae alba, Fructus Aurantii, Atractylodes, Radix Glycyrrhizae, Rhizoma Smilacis glabrae, and Radix Aconiti lateralis preparata could lower peripheral blood Th1 cells and elevate Th2 cells, and prevent the relapse of MS possibly by reducing Th1 cells and elevating Th2 cells. Especially Radix Aconiti lateralis preparata played important roles in aforesaid changes of Th1 and Th2.

Aconitum ; chemistry ; Atractylodes ; chemistry ; Bupleurum ; chemistry ; China ; Drugs, Chinese Herbal ; therapeutic use ; Fruit ; chemistry ; Glycyrrhiza ; chemistry ; Humans ; Multiple Sclerosis ; therapy ; Paeonia ; chemistry ; Plant Roots ; chemistry ; Rhizome ; chemistry ; Smilacaceae ; chemistry ; T-Lymphocyte Subsets ; drug effects