20 patients with myofascial pain dysfunction syndrome(MPDS)were were treated by NTI-tss splint only during night for 2 months.After treatment,VAS decreased(P <0.01)and active maximum mouth opening degree increased(P <0.01).NTI-tss splint is ef-fective in the treatment of MPDS.Its side effect needs to be further observed.

Background Retinal neovascularization is associated with various disorders.Studying the pathogenesis of retinal neovascularization is of important significance.Oxygen-induced retinopathy(OIR) mouse model is a common animal model for the study of retinal neovascular diseases.However, conventional modeling methods usually cause high animal mortality and low rate of success.Objective This study aimed to establish a modified method of mouse OIR model.Methods Eighty 1-week-old SPF C57BL/6J mice were randomly divided into normal control group and OIR group with 40 mice for each.The newborn mice of the normal control group were kept in a normal air environment with their breast-feeding mothers, but the mice of postnatal 2 days (P2) in the OIR group were raised with two litters per cage until P7.The P7 mice exposed to oxygen tank containing 80％ oxygen together with one or another mother mouse alternately daily for 5 days and then returned to the normal air environment.The success rate of modeling,mortality rate of maternal mice and survival rate of immature mice were evaluated.The mixed solution of fluorescein isothiocyanate (FITC) and PBS with 4％ paraformaldehyde was infused into the hearts of P12, P14,P17 and P21 mice and the eyeballs were obtained after the mice were sacrificed for histopathological examination of retinas and preparation of retinal flatmounts.The number of vascular endothelial cells extending inner limiting membrane was counted and the distribution of retinal vessels was evaluated.The use and care of the animals complied with the Statement of ARVO.Results The survival rate of the neonatal mice was 100％ both in the normal control group and the OIR group,and the survival rate of maternal mice was 85.7％ in the OIR group.Retinal new vessels were found in the mice of the OIR group,with the success rate of modeling 100％.The retinal vessels distributed from optical disc toward periphery in P14 mice in the normal group.However,in the OIR group,non-perfusion area at the posterior pole was seen in P12 mice,new blood vessels at the periphery were found in P14 mice, neovascularization at the junction area between vascular area and non-perfusion area as well as leakages were exhibited in P17 mice,and less non-perfusion area and new vessels were seen in P21 mice.Retinal inner limiting membrane was smooth in the mice of the normal group, and the vascular endothelial cell nucleus extending inner limiting membrane were seen in P12 mice and peaked in P17 mice.The vascular endothelial cell nucleus were (11.44±2.01), (31.24±1.50) and (9.23-±1.12)/slide in P14, P17 and P21 mice in the OIR group,which were significantly more than (0.27±0.14) , (0.30±0.11) and (0.32±0.16)/slide in P14, P17 and P21 mice in the normal group (t=47.90,61.30,40.70,all at P＜0.05).Conclusions The method of OIR modeling is modified by alternating maternal mice,exposing to 80％ oxygen-nitrogen mixture gas and cohabitating immature mice.Modified modeling method is simple with the low death rate of maternal mice and stable OIR phenotype.

Combined Modality Therapy ; Digestive System Surgical Procedures ; methods ; Gastrointestinal Neoplasms ; surgery ; Humans ; Treatment Outcome

Objective To investigate the effective of FGF-23(R176Q)overexpression of adult mouse mandible formation and mineralization.Methods CT scanning,serological examination,HE staining,immunohistochemical staining and RT-PCR analysis were used to compared mandible of FGF-23(R176Q)transgenic mice(TG group)and wild type mice(WT group),serum calcium and phosphorus concentrations mandibule formation and mineralization were analysised.Results Serum calcium,phosphorus and 1, 25(OH)2 D3 concentrations in WT group were significantly higher than that in TG group,the difference was statistically significant (P <0.05);the Ob.s/B.Pm TG group was lower than that of WT group,the difference was statistically significant(P <0.05);the positive percentage of Biglycan in TG group was higher than that in WT group,the difference was statistically significant(P <0.05);the OCN and type I collagen mRNA levels in the WT group were significantly higher than that in TG group,the difference were statistically significant(P <0.05).Conclusion FGF-23(R176Q)overexpression can inhibit the formation of mandible,reduce the formation of mineralized,and reduce the development of the mandible.

Objective To investigate the relationship between pulse wave velocity and cardiovascular risk factors in prehypertensive subjects. Methods We selected 195 normotensives and divided them into two groups, which was 90 subjects with optimal blood pressure(BP

This study was aimed to observe the effect of emodin on cisplatin-induced renal tubular epithelial cells (NRK-52E) injury,in order to explore its possible molecular mechanisms.Firstly,effects of emodin on cisplatin-induced morphological changes in NRK-52E cells were observed.Secondly,the apoptosis-related protein expression of Caspase-3 and cleaved Caspase-3 were detected after the treatment of cisplatin alone or cisplatin together with emodin by western blot.Then,the expression of microtubule-associated protein 1 light chain 3 (LC3) Ⅱ/Ⅰ was detected after the treatment of emodin or rapamycin at different time points by western blot.Changes of pmRFP-LC3 fluorescent particles were observed by fluorescence microscopy.And effects of rapamycin on cellular morphological changes were observed in the environment of cisplatin.Finally,effects of emodin on the activation of AMPK and mTOR signal pathway were further observed,which is considered as the upstream of autophagy signaling pathway.The results showed that cisplatin can induce morphological changes in NRK-52E cell,which was obviously ameliorated by the intervention of emodin.Additionally,the increased protein expression of cleaved Caspase-3 induced by cisplatin was obviously reduced after the intervention of emodin.The LC3-Ⅱ/LC3-Ⅰ ratio was significantly increased after the treatment of emodin or rapamycin at different time points.Rapamycin can significantly ameliorate NRK-52E cell apoptosis induced by cisplatin.Simultaneously,the number of pmRFP-LC3 fluorescent particles increased after the treatment of emodin.As the extension of time by intervention of emodin,the protein expression of p-mTOR was significantly reduced.In contrast,the protein expression of p-AMPK was significantly increased.It was concluded that emodin can ameliorate cisplatininduced apoptosis in NRK-52E cells.Its potential mechanism may be attributed to the activation of autophagy by regulating AMPK/mTOR signaling pathway.And thus,it played a role in renal protective effects.

Objective To investigate the effect of laparoscope combined with ureteroscope for the treatment of hepatolithus. Methods 98 patients were randomly divided into two groups:the study group(laparoscope combined with ureteroscope group) and the control group (the open surgery group). The mean blood loss, the recovery of the stomach and intestines function, hospital stay and the rate of complications were observed. Results Compared with the open surgery group,the mean blood loss,the recovery of the stomach and intestines function,and hospital stay in the study group had statistically significant difference (all P＜0. 05). Compared with the open surgery group, the time of surgery and the patients carryingT tubes in the study group had statistically significant difference (all P＜0. 05). Compared with the open surgery group, the rate of gallstone removal、the rate of gallstone residual and the rate of injures in the study group had no statistically significant difference(all P＞0. 05). Conclusion Laparoscope combined with ureteroscope in patients with hepatolithus had the advantage of less trauma, faster postoperative recovery and fewer complications. It was safe and effective,so it was worth to recommend its clinical use.

Aim ?3 adrenoceptor(?3-AR) is predominantly expressed in white and brown adipose tissue.?3 adrenoceptor agonist stimulates the lipolysis of white adipose tissue and non-shivering thermogenesis of brown adipose tissue to exert its antiobese action.Moreover,?3 adrenoceptor agonist increases the sensitivity and response of adipose tissue to insulin and it play an important role in the treatment of type 2 diabetes.In addition,?3 adrenoceptor agonist can also relax gastrointestinal spasm and show cardiovascular effect in human heart and vascular tissues.There has been increasing concerned in the study and development of ?3 AR agonists since 1983.Up to date,32 kinds of ?3 AR agonists have been identified.The development of ?3 AR agonists can provide new strategy and approach for pharmacotherapy of obesity and diabetic mellitus and will have Spaciously the used out look in the field of medicine.

Objective To survey the epidemiology of chronic kidney disease (CKD) among elderly people in two districts of Jiangsu province, China. Methods A total of 1404 residents aged 60 years or older in Huaian and Suzhou city in Jiangsu province were randomly recruited from the community population. All the people were screened for albuminuria (increased morning spot urine albumin-to-creatinine ratio, UACR) and reduced renal function (decreased eGFR by simplified MDRD equation). Urinary creatinine and albumin, serum creatinine, uric acid, cholesterol,triglyceride, low density lipoprotein-cholesterol, high-density lipoprotein cholesterol and fasting blood (1.73 m2) -1] and/or albuminuria (UACR ≥30 mg/g). The associations between healthy characteristics and indicators of kidney damage were examined. Results A total of 1316 (93.7%) elderly people completed the study. The prevalence of CKD was 32.3% and the awareness rate was only 9.6%. Albuminuria and reduced renal function were found in 30.2% and 3.2% of subjects respectively. The Logistic regression model showed that age, gender, hypertension,hypercholesterinemia and hyperuricaemia were independently associated with CKD. Systolic blood pressure (SBP) of 140-159 mm Hg exhibited a lower adjusted OR value (0.675) for CKD, while SBP of 160-179 mm Hg and of at least 180 mm Hg exhibited higher adjusted OR values (1.330 and 1.146). Similarly, FBG of 5.6-6.9 mmol/L exhibited a lower adjusted OR value for CKD as compared to FBG of at least 7.0 mmol/L (0.628 vs 1.941). Conclusions The prevalence of CKD in elderly people of Jiangsu province is quite high. Age, gender, hypertension,hypercholesterinemia and hyperuricaemia are independent risk factors for the development of CKD.

Robotic system helps surgeons in performing surgery. Currently Da Vinci system is the most popular. Da Vinci system has been used for the stomach and bowel diseases in 27 cases(18 cases of stomach and 9 cases of colon and rectum) in the Zhongshan Hospital, Fudan University. Accurate preoperative staging is crucial, and Da Vinci system is advantageous in lymph node dissection, preservation of nerve plexus, and complete resection of mesorectum. Adoption of gastrointestinal tract reconstruction technique should depend on the operation and experience in surgery. Though Da Vinci system has limitations and the cost is high, it is believed to be the future trend.
Digestive System Surgical Procedures ; methods ; Humans ; Robotics ; methods