1.Effects of mechanical stretch on expression of pentraxin-3 mRNA and protein in A549 cells
Huaqing SHU ; Qingping WU ; Lei YANG ; Hong ZHANG ; Shanglong YAO
Chinese Journal of Anesthesiology 2008;28(11):1023-1025
Objective To evaluate the effects of mechanical stretch on pentraxin-3(PTX-3)mRNA and protein expression in human alveolar epithelial cells (A549 cells).Methods The human lung epithelial adenocarcinoma cells A549(A549 cells)were purchased from cell biology laboratory,Tongji Medical College,Huazhong University of Science and Technology.The cultured A549 cells were inoculated on collagen Ⅰ BioFlex plates and divided into 5 groups(n=3 wells each):group Ⅰ normal control;groupⅡsham mechanical stretch;group Ⅲ mechanical stretch;groupⅣsiRNA and groupⅤ siRNA+mechanical stretch.In group Ⅲ the cells underwent square cyclic mechanical stretch for 4 h using the Flexercell Systcm.In group Ⅳ the cells were transfected with chemosynthetic PTX-3 specific siRNA by RNAi technique.In group Ⅴ at 24 h after being transfected with PTX-3 siRNA the cells underwent mechanical stretch for 4 h.In groupⅡ mechanical stretch of the cells were prevented by Flexstep.The expression of PTX-3 mRNA in the cells was detected by real-time PCR and the expression of PTX-3 protein in the culture media was determined by Western blotting.Apoptosis of the cells was measured bv flow cytometry(Beeten-Dickinson,USA).Results PTX-3 mRNA and protein expression was signlficantly up-regulated by mechanical stretch in groupⅢand decreased by transfection with siRNA in group Ⅳand Ⅴ as compared with group Ⅰ andⅡ(P<0.05 or 0.01).The apoptosis ratio was significantly higher in group Ⅲ and Ⅴ than in groupⅡ and was significantly lower in group Ⅴ thanin group Ⅲ(P<0.01).Conclusion Mechanical stretch can up-regulate PTX-3 mRNA expression in A549 cells.
2.An outbreak and treatment of Norwegian scabies occurred in a hospital
Ronghua WU ; Xiaoting LEI ; Hong LIN ; Hui SUN
Chinese Journal of Infection Control 2015;(1):57-59
Objective To investigate the causes and treatment measures of an outbreak of Norwegian scabies oc-curred in a hospital.Methods In May 2013,an outbreak of Norwegian scabies among health care workers(HCWs) occurred because of the misdiagnosis of a patient with Norwegian scabies,epidemiological investigation was carried out by healthcare-associated infection(HAI)control department,medical intervention and disinfection and isolation measures were performed.Results A total of 27 HCWs and patients’relatives developed Norwegian scabies.After active medical treatment,patients’condition improved;all appliances used by patients were cleaned and disinfected after being wrapped and sealed with plastic bags for one week.Epidemic trend of infection was under control and no new case was found.Conclusion With highly contagious,Norwegian scabies can be spread in local area,it is neces-sary to improve HCWs’diagnostic ability to this disease and take effective measures to prevent the epidemic once HAI occur.
3.Three-dimensional finite element stress analysis in periodontal tissue of left mandibular incisor and first molar with Richtts Arch
Zhenzhong KANG ; Min HU ; Hong WU ; Lei LIU
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To investigate the stress in the periodontal tissues using three-dimansional finite element method when Richtts Arch was used.Methods Three-dimensional finite element models of the lower left central incisor and first molar were set up by means of CT.Stress distribution in root,PDL and alveolar bone,and the tendency of the tooth movement were obtained by calculation under Richtts Arch.Results The value of the force at the left lower incisor was -0.34 Newton and the value of the force at the left lower molar was 0.65 Newton.The molar model revealed that the tensile stress concentration was at the mesial root cervix and the compressive stress was at the distal root cervix.The incisor model showed that the tensile stress was concentrated at the lingual cervix and the compressive stress was concentrated at the apical tip.The arch wire exerted intruding force,lingual force and the incisor tended to lingual movement and intruding movement.The molar had the tendency of lingual movement and distal tipping movement.Conclusion The anchorage molar will incline mesially when Richtts Arch is used in treatment of deep overbite,the additional torque must be used in the anchorage molar.
4.Local impedance measurement method for monitoring and evaluation of injured muscle
Xu WU ; Lei WANG ; Yongjun WANG ; Hong SHA
International Journal of Biomedical Engineering 2014;37(1):26-30
Objective To establish a new methodology of evaluating rehabilitation status of local muscle injured by measuring the impedance and phase information.Methods An evaluation method was set up,which take symmetrical parts of injured muscle as control group during the measurement.Impedance and phase values of injured muscle were extracted with local impedance measurement method before and after rehabilitation to evaluate muscle recovery.Results Injured muscle tissue impedance values decreased significantly.In the process of rehabilitation of injured muscle,the impedance and phase values of the injured muscles were gradually approaching that of healthy muscle,and then achieved basically the same as reference values after rehabilitation.Conclusions Local impedance measurement method may provide a new,non-invasive and convenient evaluation method for the recovery of injured muscle tissue,and it has good prospects for clinical application.
5.Hand hxgiene compliance rates of health care workers in general hospitals from 20 1 0 to 20 1 2-A sxstem review
Xiaoting LEI ; Hong LIN ; Hui SUN ; Ronghua WU
Chinese Journal of Infection Control 2014;(6):339-344,352
Objective To assess the prevalence of hand hygiene compliance rates of health care workers (HCWs) at general hospitals in China in 2010-2012. Methods Literatures about hand hygiene compliance of HCWs from 2010 to 2012 were retrieved from China Biology Medicine disc (CBM),China National Knowledge Infrastructure (CNKI),Wan Fang database,VIP ,and PubMed database ,Comprehensive Meta Analysis V2 software and Stata 1 2 .0 software were adopted to conduct statistical analysis . Results Ninety literatures were selected with heterogeneity (Q= 48 118.32,P<0.01),random effect model was used. The overall hand hygiene compliance rate of HCWs was 47.83% (95% CI:43.27% -52.42% );When stratified by occupation group,the overall compliance rate of doctors,nur-ses,and unclassified HCWs was 40.36% (95% CI:35.42% -45.49% ),46.70% (95% CI:41.81% -51.65% ),and 40.72% (95% CI:27.75% -55.13% )respectively. According to subgroup analysis,there was no statistical difference in compliance rate between doctors and nurses(Q= 3.12,P>0.05);the compliance rate after patient contact was higher than before patient contact (54.33% [95% CI :44.76% -63.59% ]vs 20.21% [95% CI:14.12% -28.06% ])(Q= 32.59, P<0.01 );hand compliance rate from field observation was higher than from covert observation (70.91% [95% CI :70.71% - 71.10% ]vs 41.20% [95% CI:37.55% -44.94% ])(Q= 247.66,P<0.01).Conclusion The overall hand hygiene compliance rate of HCWs in 2010-2012 was low,in order to reduce the risk of healthcare-associated infec-tion,hand hygiene compliance of HCWs need to be increased .
6.Application of negative pressure drainage on nasal septum recons tructomy by endoscopic.
Huijun REN ; Lei TONG ; Goumin WU ; Jianhe HONG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(22):1765-1768
OBJECTIVE:
To investigate the clinical effect of negative pressure drainage after septum surgery.
METHOD:
One hundred and two cases with septum deviation were randomly divided into two groups, i. e. experimental group and controlling one. With 51 cases in each. Degree of comfort and complication of two groups were compared.
RESULT:
The cases in experimental group showed significantly relieved postoperative reaction with negative pressure drainage, when compared with that of controls, the incidence of complication were not increased.
CONCLUSION
Negative pressure drainage can be taken as one of the ideal materials for hemostasia after septum surgery.
Drainage
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Endoscopy
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Humans
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Incidence
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Nasal Septum
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surgery
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Nose Deformities, Acquired
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Postoperative Period
7.Clinical Analysis of 22 Cases of Basaloid Squamous Carcinoma
Lei WANG ; Lei ZHANG ; Qiuli WU ; Xuefeng KAN ; Zhongli ZHAN ; Leina SUN ; Hong ZHU ; Changli WANG
Chinese Journal of Clinical Oncology 2010;37(5):280-283
Objective: To discuss the clinical features of basaloid squamous carcinoma(BSC)and the factors relating to its prognosis and to compare patient survival between poorly differentiated squamous cell carcinoma(PDSC)and BSC. Methods: Clinical and pathological data of BSC and PDSC cases seen in our hospital between January 2004 and December 2008 were reviewed. Results: There were no statistical differences in demographic and clinical features between PDSC and BSC patients,with the exception that a larger proportion of BSC patients were female(P=-0.001).Additionally,higher tobacco consumption was observed among BSC male patients (P=0.003).There were no significant differences in survival rate between BSC and PDSC groups(X2=0.03,P=0.5470).The median survival time of BSC and PDSC patients was 19 months and 30 months,respectively.The 4-year survival rate was 22.4%and 36.1%,respectively(u=0.740,P=0.230).No significant difference was found in survival rate between stage Ⅰ and stage Ⅱ patients(X~2=0.109,P=0.2974).The median survival time of stage Ⅰ and stage Ⅱ patients was 19 months and 46 months,respectively;and the 4-year survival rate of stage Ⅰ and stage Ⅱ patients was 47.3% and 45.2%,respectively(u=0.122,P=0.450).Using Cox proportional hazard model,we found that surgical types and clinical stages of BSC were correlated with its prognosis.Compared with that of patients who received lobectomy,the postoperative mortality hazard of patients who received pneumonectomy and segmentectomy was increased by 1.379 times(P=0.031)and 1.634 times(P=0.061),respectively.A more advanced clinical stage was associated with an increase in the postoperative morta,ty hazard ratio(X~2=14.12,P=0.000).The postoperative mortality hazard of patients of stage Ⅲ and stage Ⅳ was 2.437 times higher than that of stage Ⅰ patients(P=0.018).There were no statistical differences in postoperative mortality risk between stage Ⅰ patients and stage Ⅱ patients(P=0.057). Conclusion: Compared with that of PDSC,the incidence of BSC is higher among females.However,there is no difference in the prognosis between BSC and PDSC.BSC can be treated with the same therapies as those for other types of non-small cell lung cancer(NSCLC).
8.Comparison of postoperative analgesic effect of flurbiprofen axetil and parecoxib sodium in patients with humeral shaft fracture in painless ward
Qianjin YU ; Lei HONG ; Meijing DOU ; Guiyou WU ; Guoqiang CHEN ; Lei CHEN ; Wenfeng LI
Chinese Journal of Postgraduates of Medicine 2021;44(4):289-293
Objective:To compare postoperative analgesic effect of flurbiprofen axetil and parecoxib sodium in patients with humeral shaft fracture in painless ward.Methods:All of 200 hospitalized humeral fractures patients were retrospectively studied in the painless ward of the Forth Medical Center of PLA General Hospital from January 2017 to September 2019 , the clinical effects of flurbiprofen axetil and parecoxib were compared.Results:Postoperative visual analogue scale (VAS) scores after 3 d of two groups of patients were significantly lower, compared with preoperative results: (4.26±0.96) scores vs. (6.09±1.38) scores, (4.04±1.19) scores vs. (6.04±1.11) scores, and the differences were statistical significantly ( P<0.01). Postoperative VAS score after 3 d of two groups had no statistical significance ( P>0.05). Two groups had different degree of adverse reactions after operation, and flurbiprofen axetil group had singnificant gastrointestinal adverse reaction: 22 patients vs. 3 patients( P<0.05). The number of patients in the parecoxib group were more than that in the flubiprofen axetil group without troubled sleep: 20 patients vs. 8 patients. Two groups of patients were satisfied with the pain care during hospitalization. Conclusions:Two analgestic drugs can obtain obvious analgesic effect in the treatment of bones surgery. The side effects and sleep disturbance in the flurbiprofen axetil group are higher than those in the parecoxib group. Good pain control can improve patients satisfaction with pain care.
9.An analysis of the examination and evaluating results in controlling and eliminating coal-burning-borne endemic fluorosis in Shanxi Province
Zhaoming WU ; Zhenghui WANG ; Pengfei LI ; Min WU ; Yulan JING ; Hong WEI ; Yanqing LEI
Chinese Journal of Endemiology 2017;36(4):261-264
Objective To master the results of examination and evaluation of coal-burning-borne endemic fluorosis in Shanxi Province,and to provide a scientific basis for further prevention and treatment of the disease.Methods In 2012-2015,the prevention and control situation and condition survey were carried out in all coalburning-borne endemic fluorosis villages of 20 counties in Shanxi Province.A county-level self-inspection and a provincial-level spot-check were conducted to investigate the changes of improved stove and correct usage of the improved stove,and the correct drying of corn and pepper for human consumption.Children aged 8 to 12 were examined for dental fluorosis.The endemic areas were selected according to the Control Standards of Endemic Fluorosis in Endemic Areas (GB 17017-2010) and Eliminating Standards of Coal-burning Type Endemic Fluorosis,and dental fluorosis children was diagnosed based on the Diagnosis of Dental Fluorosis method (WS/T 208-2011).Results Totally 3 371 villages and 716 683 households were investigated,the rates of the improved stove and the correct usage of the improved stove were 98.72% (707 502/716 683) and 98.74% (698 608/707 502);the correct drying rate of corn and pepper for human consumption was 99.85% (715 605/716 683);the dental fluorosis rate of 8 to 12 years old children and the dental fluorosis index were 6.41% (6 762/105 453) and 0.14.A total of 20 counties in the province,9 counties had reached the control standards,11 counties had reached the elimination standards.Provincial and municipal level checked a total of 57 towns,157 villages,and the results of the overall compliance rate was 87.26% (137/157).Conclusions The prevention and control effect is obvious on coalburning-borne endemic fluorosis in Shanxi Province.Disease surveillance,health education,the management and maintenance work on defluoridation stoves are keys in control of coal-burning-borne endemic fluorosis.
10.Definition of home-based rehabilitation mode for patients with stroke
Lei CHENG ; Jianping WU ; Hong ZHANG ; Dongsun WU ; Yongxiang ZHOU ; Xiaoqing LI
Chinese Journal of General Practitioners 2017;16(3):179-183
Based on the experiences in Quyang Community Health Service Center,and through focus group discussions and literature research,the home-based rehabilitation mode for stroke patients in community was developed.With the principles of safety and effectiveness,rehabilitation physicians and family physicians are responsible for rehabilitation program,while rehabilitation therapists and community nurses implement the rehabilitation program.The rehabilitation services are provided for patients and their families at home with the community service resources,including counseling services,rehabilitation aids and other cares according to their needs and demands.The definition and contents of the mode provide a basis for further clarification and operation of home-based rehabilitation for stroke patients in community.