Objective To evaluate the efficacy of sleep regulation technique of thought imprint psychotherapy in lower resistant state (TIP) for the treatment of primary insomnia.Methods 45 hospitalized outpatients with primary insomnia from sleep medical clinic of Guang'anmen Hospital were randomized at a 2 ∶ 1 ratio into a therapy group and a control group using random table.In the therapy group,patients received sleep regudation technique of TIP twice per week.In the control group,patients were asked to take zopiclone 3.75mg per night 30 minutes before bedtime.And the maximum dose can be 7.5mg according to their condition.The treatment lasted one month.Pittsburgh sleep quality index (PSQI) was used to assess sleep quality.Results ① After 4 weeks treatment,the clinical efficacy showed significant difference between the two groups (therapy group was 80％,control group was 33.3％).② Inner group comparison:therapy group was significantly improved in PSQI sleep quality (1.20 ± 0.71),sleep latency(1.53 ±1.01),sleep time(1.23 ±0.82),sleep efficiency(0.90±1.21),sleep disorders (1.30±0.65),sleep medication(0.13±1.36),daytime function (1.67±0.99),total score(8.30±3.19),(P＜0.05); the control group was significantly improved in the PSQI sleep quality(1.60±0.91),sleep latency(1.87±0.64),(P＜0.05).③ Inter group comparison:after treatment,compared to the control group,the therapy group was superior in the sleep latency,sleep time,sleep efficiency,sleep medication,daytime function,and total score ofPSQI (P＜0.05).Conclusion Sleep regulation techmique of thought imprint psychotherapy in lower resistant state can improve sleep quality of primary insomniacs.

Objective To evaluate the efficacy of sleep-regulating technique of low resistance thought imprint psychotherapy (TIP3-2) for insomnia. Methods A total of 120 patients with insomnia were enrolled and randomly allocated to either a treatment group (60 patients) or a control group (30 patients). The patients in the treatment group and the control group were treated by TIP3-2 and zopiclone for 4 weeks, respectively. The therapeutic effect was evaluated using the Pittsburgh Sleep Quality Index (PSQI), polysomnography and the self-made sleep confidence scale. Results A total of 79 patients in the treatment group and 28 patients in the control group completed treatment. PSQI scores showed that overall sleep quality (1.3 ± 0.65 vs. 1.8 ± 0.50;t=6.378, P=0.000), daytime function (1.5 ± 0.89 vs. 2.1 ± 0.66;t=13.624, P=0.000) and total score (9.6 ± 3.35 vs. 12.4 ± 2.83;t=22.124, P=0.000) in the treatment group were significantly improved compared with the control group. Polysomnography showed significant difference between two groups in the times of awakening (3.4 ± 2.49 vs. 4.2 ± 4.02;t=4.196, P=0.043), and the percentages of sleep stages 1 and 2 (stage 1:35.0%± 19.42%vs. 43.0%± 23.31%, t=4.593, P=0.034;stage 2:40.3%± 16.47%vs. 34.9%± 16.57%, t=4.255, P=0.042). The total score of the sleep confidence scale in the treatment group showed a significantly improvement compared with the control group (8.6 ± 5.85 vs. 12.8 ± 3.84, t=11.346, P=0.001). Conclusion TIP3-2 can improve sleep confidence, have certain efficacy for insomnia, and may be superior to zopiclone.

Physiology course has become an important teaching part of non-medical professions in medical colleges,but there are no normative teaching textbooks and the corresponding teaching syllabus for these non-medical professions,which causes teaching contents chaos and affects the teaching effect.According to their attributes and characteristics,the research group explores continuously the physiology teaching contents suitable for non-medical professions,pays attention to contents settings which are common-sense,practical,systematic and flexible,in order to promote physiology teaching development in non-medical professions.

The centres of functional experiment in whole medical colleges accept many new teachers every year,and it is a problem how to improve the new teachers' teaching quality as soon as possible.We have gradually summarized an effective model of training the new teachers in functional science experiment based on the long-term effort.

Combined with the advantages of traditional teaching methods and problem-based teaching methods,this study proposed setting a teaching-need oriented,multimedia optimized and interactive teaching method and thus helped students understand life phenomenon dialectically and build their own study methods appropriately.

Purpose: To investigate the mechanism of acupuncture treatment of diabetic peripheral neuropathy. Methods: Acupuncture therapy was used to treat diabetic peripheral neuropathy, and compared with oral calcium antagonist and vitamin therapy by random control observation.Electromyography was performed for analysis at the same time. Results: Acupuncture treatment alleviated symptoms such as extremity numbness, pain and paresthesia in varying degrees in diabetic patients with peripheral neuropathy. The results of electromyography showed a marked improvement in motor and sensory conduction velocities. Conclusion: It is indicated that acupuncture therapy is markedly superior to oral calcium antagonist and vitamin therapy in clinical effect on diabetic peripheral neuropathy, and electromyographic recovery.

BACKGROUND: Fructose-1,6-diphosphate (FDP) of certain dosage plays a protective role in the pancreatic islets damaged by interleukin-1 beta (IL-1β), and there are different effects of FDP of low and high dosages.OBJECTIVE: To investigate the effects of FDP of low and high dosages on the islet cells damaged by IL-1β.DESIGN: A grouped design and controlled animal experiment.SETTING: Department of Physiology, North Sichuan Medical College.MATERIALS: The experiments were carried out in the Tumor Laboratory and Central Laboratory of Rheumatology and Immunology, Department of Surgery, North Sichuan Medical College between July 2004 and February 2006. Twenty Wistar rats within 1-3 days after birth were selected.METHODS: The pancreases of the rats were removed to collect islet cells, and then the cells were divided into normal control group, IL-1β damaged group, IL-1β+1, 25, 50 mmol/L FDP groups. The cellular activity was detected with methyl-thiazol-tetrazolium (MTT) assay, basic amount of insulin secretion and that stimulated by high glucose with radioimmunoassay, content of nitric oxide and activity of nitric oxide synthase (NOS) with nitric oxide and NOS kits, and the with [Ca2+]i with Fura-2fluorescent assay.MAIN OUTCOME MEASURES: Activity of islet cells; basic amount of insulin secretion and that stimulated by high glucose; content of nitric oxide and activity of NOS; [Ca2+]i.RESULTS: ① The activities (A values) of the islet cells in the IL-1β damaged group, IL-1β+1, 25, 50 mmol/L FDP groups were obviously lower than that in the normal control group (0.116±0.012, 0.129±0.008, 0.125±0.015, 0.120±0.016, 0.252±0.020, P ＜ 0.01). The activities (A values) of the islet cellswere not significantly different from that in the IL-1β damaged group (P ＞ 0.05) when the FDP dosage was too low (1 mmol/L) or too high (25 mmol/L). ② The basic amount of insulin secretion and that stimulated by glucose were significantly lower in the IL-1β damaged group, IL-1β+1, 25, 50 mmol/L FDP groups than in the normal control group [(237.00±22.21), (230.83±11.58), (225.16±12.46), (220.50±15.63),(425.67 ±16.85) mIU/L; (90.17 ±6.11), (96.62 ±8.64), (87.66-±8.24),(85.46±9.59), (204.50±10.78) mIU/L, P ＜ 0.01], and there were no significant differences between the FDP groups of Iow and high dosages and the IL-1β damaged group (P ＞ 0.05). ③ The NOS activity and content of nitric oxide in the supernatant were obviously higher in the IL-1β damaged group than in the normal control group [(332.07±25.34), (144.86±12.17) μkat/L;(457.64±19.29), (84.67±10.23) μmol/L, P ＜ 0.01], and those in the IL-1β+1, 25, 50 mmol/L FDP groups were not significantly different from those in the IL-1β damaged group. ④ The [Ca2+]i concentration in islet cells was obviously higher in the IL-1β damaged group than in the norrmal control group [(328.50±26.28), (73.42±1.79) nmol/L, P ＜ 0.01], but obviously lower in the IL-1β+1, 25, 50 mmol/L FDP groups than in the IL-1β damaged group [(152.72± 11.86), (216.39±15.32), (233.61±21.76),(328.50±26.28) nmol/L, P ＜ 0.01].CONCLUSION: FDP of low and high dosages can not protect the islet cells damaged by IL-1β.

Objective To investigate the neurodevelopmental outcomes and its risk factors of very low birth weight infant (VLBWI) and extremely low birth weight infants (ELBWI).Methods Data of 85 VLBWI and ELBWI hospitalized in Children's Hospital of Fudan University from October 2005 to November 2009 who had finished infant development test of Baley Scales of Infant Development Ⅱ (Bayley Ⅱ) for neurological development at corrected gestational age between 18 to 42 months were retrospectively reviewed.Twelve infants who accepted treatment in other hospital over 10 days were excluded; the rest 73 infants were divided into normal (neurodevelopment) group or abnormal group according to the definition of neurodevelopmental impairment reported by National Institute of Child Health and Human Development (NICHD) Neonatal Network.Potential risk factors of neurodevelopmental impairment were analyzed with Logistic stepwise regression.Results The mean gestational age of 73 infants was (30.4±2.3) weeks; among which 13 were smaller than 28 weeks,42 between 28 and 32 weeks,and 18 older than 32 weeks.The mean birth weight was (1208.0±208.5) g; among which 15 (20.6％) ＜1000 g,and 58 (79.4％) were between 1000 g and 1500 g.Four babies (5.5％) were diagnosed as movement retardation,and neurodevelopmental impairment occurred in 16 cases (21.9 ％),psychomotor developmental index ＜70 occurred in 6 cases (8.2％,one case complicating with cerebral palsy); mental developmental index ＜70 occurred in 2 cases (2.7％); both psychomotor developmental index and mental developmental index ＜70 occurred in 7 cases (9.6 ％,two cases complicating with cerebral palsy),and one case (1.4 ％) was cerebral palsy only.Blind in either eyes and hearing impairment requiring deaf-aid were not found in any of the 73 babies.Logistic stepwise regression showed that use of mechanical ventilation was related to neurodevelopmental impairment (OR =6.183,95％ CI:1.664-22.983,P =0.003).Psychomotor developmental index of infants who needed mechanical ventilation (77.5±15.1) was lower than that of infants did not need (87.3±15.1)(t=2.646,P=0.010).Conclusions VLBWI and ELBWI are in high risk of neurodevelopmental impairment,especially those who need mechanical ventilation.

Objective To investigate the protective role of FDP to STZ induced islest apoptosis and the potential mechanisms.Methods The pancreases of the rats were treated to collect islets cells.The cells were incubated with STZ with/or FDP.Cell morphology,insulin secretion,HO-1 activity,CO content,SOD activity,GSH-px activity,iNOS activity were examined.No conetent and apoptotic percentage was detected.Results HO-1 activity and CO content of the normal control group were low.STZ induced a significant decrease of cell activity and insulin release,flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of STZ,FDP obviously improved the islets cellular activity damaged by STZ,basic amount of insulin secretion and stimulated by high glucose were improved(P

Colitis, Ulcerative ; complications ; drug therapy ; Humans ; Male ; Middle Aged ; Psoriasis ; complications ; drug therapy ; Pyoderma Gangrenosum ; drug therapy ; etiology