AIM: To evaluate the relationship between axial length ( AL ) , corneal and lens refractive power, and the refractive error in children.
METHODS:Totally 44 children 88 eyes with refractive error who underwent retinoscopy with cycloplegia, to be measured spherical equivalent refractive error. Axial length was measured by a noncontact optical biometry ( ZEISS IOL-Master) , and corneal K and anterior chamber depth ( ACD) were also measured by the same machine. The refractive power of the lens was calculated by using the SRK formula. The patients were divided into 3 groups, myopia (SE<0), hyperopia (SE>+0. 50D) and emmetropia ( 0 to + 0. 50D ). Linear Correlation and Regression were used to evaluate the correlation among the optical parameters.
RESULTS:Totally 44 subjects, 88 eyes, average 9. 04±2.39 years, spherical equivalent (SE) -3. 50D to +8. 75D. Hyperopic AL was shorter than the other two groups ( P<0.05), same trend in lens power. No significant differences among the 3 groups in corneal K and ACD. There was a negative correlation between age and SE, SE and AL, SE and lens refractive power, and there was a positive correlation between age and AL, age and lens power.
CONCLUSION:As the children's growing up, SE trends to myopia, and AL becomes longer, and lens power is stronger.

Objective To explore the treatment effect and mechanism of A-NK cells on the subcutaneous trans?planted tumor of oral tongue squamous cell carcinoma in nude mice through observing the external growth and hyperplasia of A-NK cells and to provide theoretical evidence for squamous cell carcinoma treatment. Methods A-NK cells and NA-NK cells were both derived from healthy human peripheral blood and cultured in vitro. Cell growth was observed under micro?scope. The squamous cell carcinoma model in nude mice was established through subcutaneous implanting of Tca8113 cells. Then they were randomly assigned into three groups who were injected with either saline solution, or A-NK cells or NA-NK cells paraneoplastically. All animals were sacrificed after 33 days when tumor were isolated then weight and change in tumor size were assessed. Finally curve of tumor growth was drawn. Results Under the microscope, the proliferation of A-NK cells peak in 15 days and NA-NK cells peak in 12 days. After 3 weeks, the number of A-NK cells increased by 39.33 times while the number of NA-NK cells increased by 16.33 times. The Volume of tongue squamous cell carcinoma in saline solu?tion group was larger than that in A-NK cells and NA-NK cells groups, and volume in the NA-NK cells group was larger than that in A-NK cells group. The volume of tongue neoplasms in different groups, time, and interaction effects are statisti?cally significant (P<0.01). The tongue neoplasms weight in the saline solution group was greater than that in the A-NK cells and NA-NK cells group, and the weight in NA-NK cells group was greater than that in A-NK cells group, and the difference are statistically significant (P<0.05). Conclusion A-NK cells and NA-NK cells can significantly inhibit the subcutane?ous transplanted tumors in nude mice and the anti tumor effect of A-NK group is stronger than NA-NK.

Peroxiredoxin 1(Prdx1)belongs to peroxide oxidoreductase protein(peroxiredoxin, Prdx)family,which is over-expressed in multiple cancers and it play an important role in antagonizing reactive oxygen species (ROS) therefore as works as anti-oxidations. Its expression is closely related with tumor proliferation, differentiation and metastasis as well as with sensibility to radiotherapy and chemotherapy. Its structure and biological function in tumors and regulation mechanism were reviewed in this paper. It can provide evidence to screen new therapeutic targets in the treatment of tumors.

BACKGROUND:The cytokine has an effect of immunoregulation and immediate induction in the reconstruction of periodontal tissue. At present, the role and mechanism of hepatocyte growth factor involving the reconstruction of periodontal tissue under orthodontic force are stil unclear.
OBJECTIVE:To explore the mechanism underlying hepatocyte growth factor in the tooth movement and periodontal tissues remodeling under the inflammation periodontal tissue condition.
METHODS:Thirty Sprague-Dawley rats, aged 8 weeks, were used to establish periodontitis model. The obtained model was randomly divided into two groups:inflammatory control group and inflammatory force group. In the force group, rats were treated with the fixed orthodontic appliance by 50 g forces in the maxil ary first molars. After 1, 3, 5, 7 and 14 days of tooth movement, five rats were sacrificed respectively. Hematoxylin-eosin staining and immunohistochemistry methods were used to analyze the expression and distribution of hepatocyte growth factor in the periodontium for rats at different tooth movement stages.
RESULTS AND CONCLUSION:Hematoxylin-eosin staining results showed that, remodeling of periodontal tissues existed in al groups. (2) The immunohistochemical results showed that hepatocyte growth factor had positive expression in periodontal tissue, and the distribution was even in the control group. In the force group, hepatocyte growth factor expression was increased and reached the peak on day 5, then began to decline. Osteoblast, fibroblast and osteoclast were strongly expressed. The findings indicate that, hepatocyte growth factor is involved in the periodontal tissues remodeling under orthodontic force, and inflammation can increase the expression of hepatocyte growth factor in periodontal tissue.

Objective To study the protective effects of curcumin on LPS-induced acute renal injury to offer the clinical evidence of treatment in mice. Methods Fifty-two mice were randomly divided into 3 groups, the control group (n=12), the LPC group (n=20) and the treated group (LPS + curcumin group, n=20). LPS (5 mg/kg) was injected intraperitoneally into the mice of the LPS group. Curcumin (20 mg/kg) was injected intraperitoneally after an intraperitoneal injection of LPS (5 mg/kg) to the mice of the treated group. The TNF-? level of the renal tissue, the serum level of urea nitrogen (BUN) and serum creatinine (Cr) were determined in 3, 6, 12 and 24 h after injection. The expression of TLR2 mRNA was detected with semi-quantitative RT-PCR and the histopathological changes of the kidneys were studied. Results The TNF-? level of renal tissue and the serum levels of BUN and Cr were significantly increased in 3 h after injection in the LPS group and reached the peak at 6 h. The significant increase of the parameters lasted for 24 h. The changes of the expression of TLR2 mRNA were similar to those of TNF-?, BUN and Cr. The histopathological changes of the kidneys included renal tubular necrosis and detachment, formation of casts, glomerular ischemia and shrinkage and infiltration of large amount of PMN in LPS group. In the theated group, the tissue level of TNF-?, the serum levels of BUN and Cr and the expression of TLR2 mRNA were significantly decreased in 3, 6 and 12 h after injection and the histopathological changes were obviously alleviated. Conclusion Curcumin can alleviate LPS-induced acute renal injury and exerts good protection on the functions of the kidneys.

Objective To observe the clinical efficacy and safety of dexmedetomidine on analgesic effect in patients after thyroid surgery.Methods A total of 102 patients after thyroid surgery were divided into control group and treatment group,each group 51 cases.Patients in both groups were given thyroid operation.Patients in both groups were given sulfentanyl 10 μg + 0.375％ ropivacaine mixture 20 mL before operation,granisetron hydrochloride injection 5 mg by intravenous drip,once a day after operation.Control group was given midazolam hydrochloride injection 0.03 mg · kg-1 by intravenous drip before operation.Treatment group was received dexmedetomidine hydrochloride injection 1 μg · kg-1 by intravenous drip before operation.The clinical efficacy,3-endorphin (3-EP),interleukin-6 (IL-6),C-reactive protein (CRP) and adverse drug reactions in two groups were observed.Results After treatment,total effective rates in treatment and control groups were 92.16％ (47/51 cases) and 78.43％ (40/51 cases),with significant difference (P ＜ 0.05).After treatment,β-endorphin in control group and treatment group were (268.47 ± 29.58),(284.47±30.05) pg· L-1,CRP were (4.47 ±0.54),(4.07 ±0.42)mg · L-1(P＜0.05).The adverse drug reactions in treatment group were lethargy,respiratory depression,nausea and vomiting,the incidence of adverse drug reactions was 5.88％ (3/51 cases).The adverse drug reactions in control group were itching,respiratory depression,drowsiness,nausea and vomiting,the incidence of adverse drug reactions was 11.76％ (6/51 cases,P ＞ 0.05).Conclusion The analgesic effect of dexmedetomidine after thyroid surgery is effective with high safety.

This study investigated the protective effect of ATP on skeletal muscle satellite cells damaged by H2O2 in neonatal rats and the possible mechanism. The skeletal muscle satellite cells were randomly divided into four groups: normal group, model group (cells treated with 0.1 mmol/L H2O2 for 50 s), protection group (cells treated with 16, 8, 4, 2, 1, 0.5, or 0.25 mmol/L ATP for 24 h, and then with 0.1 mmol/L H2O2 for 50 s), proliferation group (cells treated with 16, 8, 4, 2, 1, 0.5, or 0.25 mmol/L ATP for 24 h). MTT assay, FITC+PI+DAPI fluorescent staining, Giemsa staining and immunofluorescence were performed to examine cell viability and apoptosis, and apoptosis-related proteins. The results showed that the survival rate of skeletal muscle satellite cells was decreased and the apoptosis rate was increased after H2O2 treatment (P<0.01). Different doses of ATP had different effects on skeletal muscle satellite cells damaged by H2O2: the survival rate of muscle satellite cells treated with ATP at 4, 2, or 1 mmol/L was increased. The protective effect was most profound on cells treated with 2 mmol/L ATP. Immunofluorescence showed that ATP could increase the number of Bcl-2-positive cells (P<0.01) and decrease the number of the Bax-positive cells (P<0.01). It was concluded that ATP could protect skeletal muscle satellite cells against H2O2 damage in neonatal rats, which may be attributed to the up-regulation of the expression of Bcl-2 and down-regulation of Bax, resulting in the suppression of apoptosis.

Adult ; Child ; Endoscopy ; Fibrous Dysplasia of Bone ; pathology ; surgery ; Humans ; Male ; Nose ; surgery ; Orbit ; pathology

Blood Flow Velocity ; Coronary Angiography ; Coronary Vessels

Aleutian mink disease parvovirus (AMDV) causes a persistent infection associated with immune complex disease, hypergammaglobulinemia, and high levels of antiviral antibodies. Despite the presence of an antibody, the virus is not cleared in vivo. Pre-existing antibodies may enhance viral infections, by Fc-receptor-mediated antibody-dependent enhancement (ADE), but the mechanism that underlies ADE has not been fully defined. Three models have been proposed, including: (1) interactions between antibody and FcR, complement C3 fragment and CR, or between C1q and C1qR, which promotes viral attachment to cells; (2) suppression of IFN-gamma-mediated host-cell antiviral gene expression by the upregulation of negative regulators of pathogen pattern recognition; and (3) the promotion of early IL-10 secretion. In addition, the role of cytokine IL-6 in ADE mediated disease development is discussed, to facilitate a better understanding of the pathogenesis of AMDV infection, as well as give insights into rational vaccine design approaches.
Aleutian Mink Disease ; immunology ; virology ; Aleutian Mink Disease Virus ; genetics ; immunology ; Animals ; Antibodies, Viral ; immunology ; Antibody-Dependent Enhancement ; Mink ; immunology ; virology