3.Inhibitory effects of total flavonoids of scutellaria baicalensis georgi on S_(180),Hep-A-22 and Bcap-37 tumor cells
Juan SHENG ; Zhen YANG ; Hongliu JIANG ; Tie HONG
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To study the inhibitory effects of total flavonoids of scutellaria baicalensis georgi(TFSB) on S180,Hep-A-22 and Bcap-37 tumor cell proliferation in vitro and on S180,Hep-A-22 in mice bearing tumor in vivo.Methods In vitro,S180,Hep-A-22 and Bcap-37 cells were divided into control group and TFSB groups(12.5,25.0,50.0,100.0 mg?L-1).The inhibitory effects of TFSB on proliferation of S180 and Hep-A-22 were measured by XTT colorimetric assay,and Bcap-37 cells were measured by MTT colorimetric assay.In vivo,the mice bearing tumor were divided into control group,CTX group(30 mg?kg-1),high,middle,low doses TFSB groups(200,100,50 mg?kg-1).After the mice bearing S180 and Hep-A-22 tumor cells were treated with TFSB for 15 d,the tumor weights were measured,the inhibitory rates of S180 and Hep-A-22 were calculated and survival of Hep-A22 was measured after administration of TFSB for 10 d.Results TFSB inhibited the proliferation of S180,Hep-A-22 and Bcap-37 cells,IC50 values were 16.04,17.74 and 9.05 mg?L-1,respectively.The tumor weight of mice bearing S180 and Hep-A-22 cells in TFSB groups(200,100,50mg?kg-1) were lowered than that in control(P
4.Effects of nuclear factor-?B decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclampsia
Rong-Zhen JIANG ; Ya-Juan HUANG ; Jing-Hong GU ; Wei-Hong MU ; Han-Ping CHEN ;
Chinese Journal of Obstetrics and Gynecology 2001;0(02):-
Objective To study the effects of nuclear factor-?B(NF-?B)decoy oligodeoxynucleotide(ODN)on the preeclamptic umbilical serum induced expression of precollagen Ⅰ,Ⅲ mRNA and tumor necrosis factor-?(TNF-?)in cultured human umbilical artery smooth muscle cells (HUASMC).Methods Primary cultured HUASMC of normal pregnancy were divided into four groups: group A(HUASMC were incubated with umbilical serum of normal pregnancy);group B(HUASMC were incubated with umbilical serum of preeclampsia);group C(HUASMC were transfected with NF-?B cis decoy ODN 48 h before incubation with umbilical serum of preeclampsia);group D(HUASMC were transfected with NF-?B scramble ODN 24 h before incubation with umbilical serum of preeclampsia).NF-?B cis decoy ODN and NF-?B scramble ODN were transfected with cationic lipofectamine to the latter two groups,respectively.The proliferation of human umbilical artery smooth muscle cells was evaluated by methyl thiazolyl tetrazolium and the apoptosis was analyzed by flow cytometry.The expression levels of precollagen Ⅰ,Ⅲ mRNA were detected by RT-PCR,the expression levels of TNF-? were detected by western blot.Results(1)The proliferation of group B(0.19?0.02)and group D(0.18?0.03)was significantly increased as compared with those of group A(0.11?0.02)and group C(0.14?20.02)(P0.05).(5)The expression of TNF-? of group B(0.74?0.11),group C(0.36?0.09)and group D(0.79?0.12)were significantly higher than that of group A(0.15?0.03)(P0.05).Conclusions NF-?B cis decoy ODN could down-regulate the proliferation,as well as the expression levels of precollagen and TNF-? of HUASMC induced by umbilical serum of preeclampsia.NF-?B may play an important role in the pathogenesis of placental artery abnormalities in preeclampsia.
5.Comparison of liquid-based and conventional preparations in cytologic diagnosis of pancreatobiliary lesions.
Hong JIANG ; Zi-juan QI ; Ming DU ; Xu REN ; He-ming WU ; Lin LIU
Chinese Journal of Pathology 2013;42(4):269-270
Adult
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Aged
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Aged, 80 and over
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Bile Duct Diseases
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diagnosis
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Bile Duct Neoplasms
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diagnosis
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Cholangiopancreatography, Endoscopic Retrograde
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Cytodiagnosis
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Cytological Techniques
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methods
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Female
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Humans
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Male
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Middle Aged
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Pancreatic Diseases
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diagnosis
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Pancreatic Neoplasms
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diagnosis
6.Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis.
Chang-Ming WANG ; Juan CHEN ; Ming JIANG ; Xiu-Ping XUAN ; Hong-Xiu LI
Acta Pharmaceutica Sinica 2014;49(1):142-147
This study is to investigate the effect of artesunate on transforming growth factor-beta1 (TGF-beta1) induced epithelial-mesenchymal transition (EMT) and its possible mechanism. After the in vitro cultured RLE-6TN cells were treated with TGF-beta1 then artesunate intervened on it, after 24 h, expression of the markers of mesenchymal cell was assayed using Western blotting and real-time PCR analysis. Western blotting was also used to detect the effect of TGF-beta1 on the Smad3 and Smad7 expressions of RLE-6TN cells. Morphological alterations were examined by phase-contrast microscope, and ultrastructure changes by electron microscope. Incubation of RLE-6TN cells with TGF-beta1 resulted in the up-regulation of the expression of the mesenchymal cell markers, after artesunate intervened on it, resulted in the down-regulation of the expression. Meanwhile, incubation with artesunate intervened on RLE-6TN cells could lead to the apparent down-regulation of the expression of Smad3 and up-regulation of Samd7 and the transition of RLE-6TN cells to mesenchymal-like by TGF-beta1 induction, after artesunate intervened on it, RLE-6TN cells to epithelial-like. TGF-beta1 induced epithelial-mesenchymal transition process; artesunate can inhibit TGF-beta1-induced epithelial-mesenchymal transition process, the possible mechanism is up-regulation of the expression of Smad7 and down-regulation of the expression of Smad3, meanwhile inhibits phosphorylation of Smad3.
Actins
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genetics
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metabolism
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Animals
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Artemisia
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chemistry
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Artemisinins
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isolation & purification
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pharmacology
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Cell Line
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Cell Proliferation
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drug effects
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Epithelial Cells
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cytology
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metabolism
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Epithelial-Mesenchymal Transition
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drug effects
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Idiopathic Pulmonary Fibrosis
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pathology
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Plants, Medicinal
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chemistry
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Pulmonary Alveoli
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cytology
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RNA, Messenger
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metabolism
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Rats
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Smad3 Protein
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genetics
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metabolism
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Smad7 Protein
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genetics
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metabolism
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Transforming Growth Factor beta1
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pharmacology
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Vimentin
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genetics
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metabolism
7.Study on relations between transient receptor potential vanilloid 1 and pungent property of traditional Chinese medicines.
Xing WANG ; Yan-Ling ZHANG ; Yun WANG ; Zhen-Zhen REN ; Hong-Juan BAO ; Yan-Jiang QIAO
China Journal of Chinese Materia Medica 2014;39(13):2422-2427
The five-flavor theory of traditional Chinese medicines (TCM) and the flavor efficacy generation mechanism has long been focuses and difficulties in studies on traditional Chinese medicinal properties. In this paper, by using the pharmacophore-based virtual screening technique, the authors discussed the relations between the pungent property and transient receptor potential vanilloid 1 (TRPV1) by studying the TCM components' role in regulating TRPV1 ion channel. The results showed that the matching relationship between TRPV1 agonist pharmacophore model and TCM chemical components could identify the active ingredients from pungent herbs. Therefore, the authors proposed that TRPV1 is one of the potential targets for efficient pungent herbs. The pungent property of TCMs is decided by its chemical components, and consistent with the inherited and additive characteristics.
Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Smell
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TRPV Cation Channels
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antagonists & inhibitors
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metabolism
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Taste
8.Deactivation of alveolar macrophages in neutropenic acute respiratory distress syndrome (ARDS) in children with hematologic malignancy
Juan QIAN ; Liming JIANG ; Biru LI ; Li ZHAO ; Yanwen YANG ; Hong REN ; Ying WANG
Chinese Pediatric Emergency Medicine 2010;17(4):312-314
Objective To study the alveolar macrophages (AM) activation in neutropenic ARDS in children with hematologic malignancy. Methods Thirteen patients of ARDS were prospectively enrolled into the study and divided into two groups,neutropenic (5 cases) and nonneutropenic (8 cases). Bronchoalveolar lavage fluid (BALF) samples were analyzed for differential and total cell counts, and alveolar activation marker expression (HLA-DR) was determined. Results In neutropenic patients,the BALF total cell count and the macrophage absolute count were significantly lower compared to those in nonneutropenic group ((62. 6 ±9. 6)/μl vs ( 124. 0 ± 6. 7)/μl,P < 0. 01 and (40. 8 ±4. 3)/μl vs (67. 6 ± 10. 7)/μl,P < 0. 01,respectively). As compared with the nonneutropenic group, the percentage of AM in neutropenic patients was significantly higher (65.9% ±9. 0% vs 54. 6% ± 8. 7% ,P <0. 05) ,the mean percentage of AM HLA-DR expression was significantly lower (35. 3% ± 5.8% vs 62. 2% ± 5. 8%, P < 0. 01 ). Conclusion These results suggest an alveolar hypocellularity and a deactivation of AM in neutropenic ARDS in children with hematologic malignancy.
9.Age related reference value for reticulocyte hemoglobin equivalent among children aged one to thirteen years
Yuefang WANG ; Hui YANG ; Xia WANG ; Juan LI ; Lan CHEN ; Hong WANG ; Qi CHEN ; Yongmei JIANG
Chinese Journal of Laboratory Medicine 2008;31(7):767-770
Objective To establish age related reference value for the new reticulocyte hemoglobin equivalent (RET-He) parameter in healthy children aged one to thirteen years. Methods Reticulocyte hemoglobin equivalent was measured by Sysmex XE-2100 after completing daily internal quality control in 424 healthy children and the outlying observations was handled afterward, Age related reference value anddistribution of RET-He level were described as the 2.5th and 97.5th centiles, and the equation regardingage related reference value was established through curve fitting methods. Results After grouping accordingto sex, the medians of boys and girls in RET-He were 31.30 pg,31.80 pg. Non-parametric test showed therewas no differences between the boys and girls. When the data from two group was integrated together, thefitted equations of RET-He were: P2.5 =-0.008X3+0.125X2 - 0.178X+26.456; P97.5= 0.021X2-0.184X+34.670(X refers to age) ; R2 were 0.85 and 0.90,respectively(P <0.05). There was statisticallysignificance for the above equations. Conclusions The reference value of RET-He in healthy children isdifferent from those in adult. The equations can be used to produce age related reference value. It could beuseful for anemia diagnosis, differential diagnosis and hematopoiesis screening at early stage.
10.Eukaryotic Expression of ?ig-h_3 Gene and Its Effects on Secretion of MMPs in the Human Hepatoma 7721 Cells
Juan TANG ; Jian-Li JIANG ; Hong-Wei ZHOU ; Hua XIONG ; Xiang-Min YANG ; Zhi-Nan CHEN ;
China Biotechnology 2006;0(09):-
?ig-h_3 was first identified as a transforming growth factor-beta1-inducible gene in human lung adenocarcinoma cell line. It encodes for a secreted extracellular matrix (ECM) protein, which is thought to act on cell attachment and ECM composition. Previous study showed that ?ig-h_3 were highly expressed in human hepatoma cell lines and lowly expressed in human normal hepatic cells. The present study aimed to transfect ?ig-h_3 into 7721 cells to investigate its effect on secretion of MMPs in the transfected human hepatoma cells. Full-length ?ig-h_3 gene,cloned by reverse transcription polymerase chain reaction (RT-PCR) was inserted into the eukaryotic expression vector pEGFP-C_2. The recombinant plasmid was transfected into 7721 cells with Lipofectamine2000 and Gelatin-Zymography were adopted to detect the production of MMPs in the transfected cells. Results showed that ?ig-h_3/pEGFP-C_2 recombinant expression plasmid was successfully constructed and achieved high transfection efficiency. MMPs expression of the transfected cells was promoted significantly. These results suggest that overexpression of ?ig-h_3 promoted the production of MMPs, indicating that ?ig-h_3 may play roles in the invasive and metastatic processes of hepatoma.