1.Children enuresis treated with point press.
Chinese Acupuncture & Moxibustion 2012;32(4):362-362
Acupressure
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Acupuncture Points
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Child
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Child, Preschool
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Enuresis
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physiopathology
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therapy
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Female
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Humans
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Male
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Treatment Outcome
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Urination
5.Effect of vascular endothelial growth factor on apoptosis of endothelial cells induced by hypoxia
Jun JIN ; Shanjun ZHU ; Lan HUANG ; Changqing XIANG ; Hong LI
Journal of Third Military Medical University 2001;23(2):196-198
Objective To explore the effect of hypoxia on the apoptosis of cultured human umbilical vein endothelial cells(HUVECs) and the role of vascular endothelial growth factor(VEGF) in inhibition of apoptosis. Methods ①Culture and identification of HUVECs.②Establishment of hypoxic model(0,12,24,48 h)in HUVECs.③Incubation of HUVECs with VEGF(0 ng, 100 ng) under hypoxic condition for 24 h. ④Detection of apoptosis of HUVECs with TUNEL method. Results The percentages of apoptosis were different under different hypoxic conditions. The longer hypoxic time was,the higher apoptosis percentage was.VEGF reduced the apoptosis of HUVECs induced by hepoxia. Conclusion Over-apoptosis EVCs in one of the important factors for the impairment of endothelial function. HEGF inhibits the apoptosis of HVCs and having a pretive function on them.
6.Synthesis and inhibition of NO production activities of 1,6-O, O-diacylbritannilactone derivatives
Hong ZHU ; Xiaopeng WEI ; Meihua JIN ; Nan QIN
Journal of China Pharmaceutical University 2017;48(4):440-444
Eight 1,6-O,O-diacetylbritannilactone (OABL) derivatives (compounds 1-8) were synthesized by esterification or reduction of 1-O-diacetylbritannilactone (ABL) isolated from Inula japonica.All derivatives were evaluated for their anti-inflammation activities through the determination of inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages.As results,compounds 5-8 (IC50 < 2 μmol/L) exhibited more potent inhibition of NO production activities than the lead compound OABL.
9.Effect of ecdysterone on apoptosis induced by hypoxia in HUVECs
Jun JIN ; Lan HUANG ; Shanjun ZHU ; Changqing XIANG ; Hong LI ;
Chinese Pharmacological Bulletin 1986;0(05):-
AIM To explore the effect of hypoxia on apoptosis in human umbilical vein endothelial cells(HUVECs) and the role of ecdysterone(EDS)in inhibition of apoptosis. METHODS ① Culture and identification of HUVECs;② Hypoxic model(0,12,24,48 h) in HUVECs was established. While HUVECs was incubated 24 h with EDS(100 mg?L -1 ) under hypoxic condition. Apoptosis in HUVECs was detected by TUNEL;③ HUVECs received EDS 100 mg?L -1 in normal and hypoxic condition. After 24 h, vascular endothelial growth factor (VEGF) was detected with immunohistochemical technique. RESULTS The apoptosis percentages are different under different hypoxic condition. The longer hypotic the time was, the higher the apoptosis percentage was. EDS reduced apoptosis of HUVECs. EDS could enhance expression of VEGF protein in cardiac myocytes of rat in normal and hypoxic condition. CONCLUSION The role of hypoxia in HUVECs apoptosis is more significant along with prolonging of hypoxic time. VEGF play an important role in protective effect of EDS on endothelial cell apoptosis.
10.Quality standard for Gubiling Capsules
Zhu JIN ; Hong WEI ; Bingjun LI ; Quancheng ZHAO ; Xuguo GONG ;
Chinese Traditional Patent Medicine 1992;0(05):-
AIM: To establish the quality standard for Gubiling Capsules (Penis Et Testis Canis, Radix Noto ginseng, Radix Achyranthis Bidentatae, Herba Epimedii, Radix Aucklandiae, etc.). METHODS: Radix Achyranthis Bidentatae, Herba Epimedii, Radix Aucklandiae were identified by TLC, and the ginsenoside Rg 1 content was determined by HPLC. RESULTS: Ginsenoside Rg 1 showed a good linear relationship in the range of 1.2-6.0 ?g ( r = 1.00 0 ) and average recovery was 96.9%. RSD was 0.4%. CONCLUSION: These methods are simple, accurate and specific and can be used for the quality control for Gubiling Capsules.