1.Molecular biological studies of erectile dysfunction: an update.
National Journal of Andrology 2015;21(2):99-106
Erectile dysfunction (ED), as a common male disease, seriously affects the patients' sexual life quality. Most ED patients benefit from phosphodiesterase type 5 (PDE5) inhibitors, but some refractory ED sufferers fail to respond to them. With the rapid development of molecular biology, the relevant molecular signaling pathways of penile erection and molecular pathogenesis of ED have been gradually clarified, and attempts have been made at a better management or a complete cure of ED with advanced molecular biological methods such as the gene therapy. This article presents an overview on the research progress in the molecular signaling pathways, molecular pathogenesis, and gene therapy of ED.
Biomedical Research
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Erectile Dysfunction
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genetics
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therapy
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Genetic Therapy
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Humans
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Male
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Penile Erection
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genetics
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Phosphodiesterase 5 Inhibitors
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therapeutic use
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Quality of Life
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Signal Transduction
3.Expression of EGF mRNA in renal parenchyma of congenital hydronephrosis in children
Yi YANG ; Shijun JI ; Hong GAO
Chinese Journal of Urology 2000;0(05):-
Objective To detect the expression of EGF mRNA in renal parenchyma in congenital hydronephrosis in children and to evaluate the role of EGF in causing chronic renal damage. Methods The expression of EGF mRNA in renal parenchyma, renal pelvis and PUJ tissues from congenital hydronephrosis in children was studied by means of RT PCR. Results The expression of EGF in the aff ected renal parenchyma and PUJ tissues decreased.It also decreased in the affected pelvis but the difference was not significant. The decrease of EGF expression was the most obvious in the affected renal parenchyma. Conclusions EGF expression decreased in renal parenchyma in congenital hydronephrosis and the decrease might be related to the chronic renal damage and renal atrophy caused by hydronephrosis.
4.Protective effect of FMCL on the apoptosis of PC12 cells induced by H_2O_2
Yingshi JI ; Hong LI ; Shijie YANG
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To investige the protective effect of FMCL on the apoptosis of PC12 cells induced by H_2O_2 and its mechanisms.Methods Apoptosis of PC12 cells induced by H_2O_2 at the concentration of 0.5 mmol?L~(-1).The cell activity was determined by MTT.The morpholog changes were observed by invert microscope.DNA ladder was observed through agarose gel electrophoresis.The subdiploid peaks showing cell apoptosis rate and △?m were detected by flow cytometry.Results Compared with those of the Model group the cell activity of FMCL 100、30 mg?L~(-1) group reinforced,the injury of the cell relieved.The apoptosis rate of those two groups decreased,but the△?m and the cell activity increased in a dose-dependent manner.Conclusion FMCL can inhibit the apoptosis of PC12 induced by H_2O_2 which might be correlated with the increasion of △?m.
5.The inhibitory effect of capsaicin on streptozocin-induced apoptosis of rat retinal cells
Ting, ZHANG ; Ji-hong, YANG ; Zheng, GUO
Chinese Journal of Experimental Ophthalmology 2013;(1):34-38
Background Diabetes mellitus (DM) can provoke the apoptosis of retinal cells and downregulate the expression of calcitonin gene related peptide (CGRP) in the retina.Capsaicin promotes the release of CGRP and elicits protective effects on human organs.However,whether CGRP protects retinal cells in diabetic retinopathy (DR) is still unclear.Objective The study was designed to examine the effect of capsaicin on the apoptosis of retinal cells in diabetic rats and its relationship with CGRP.Methods Forty clean healthy adult male Sprague-Dawey rats were randomly divided into the diabetes group,capsaicin pretreated group,streptozocin (STZ)control group,capsaicin control group and plain control group,with 8 rats per group.The diabetic model was established by the intraperitoneal injection of 60 mg/kg in all rats except those of the plain control group.0.4 mL of a 1% capsaicin injected at 20 mg/kg was subcutaneously injected for 3 consecutive days prior to model establishment in the capsaicin pretreated group,after which 1.2 mL of STZ was intraperitoneally injected on the fourth day.Rats from the STZ control group were administered intraperitoneally 1.2 mL of 0.1 mol/L,pH 4.5,citrate buffer.The capsaicin control group received subcutaneous injections of 0.4 mL of 1% capsaicin at 20 mg/kg for 3 consecutive days,after which 1.2 mL of 0.1 mol/L,pH 4.5,citrate buffer was administered intraperitoneally.The rats were sacrificed at the tenth week after model establishment and retinal specimens were prepared for the apoptosis assay by TUNEL staining and the quantitative analysis of caspase-3 activity.Expression of CGRP in the retina and serum was detected using ELISA.The use of experimental animals followed the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission.Results Retinal cell apoptosis was mainly localized to the retinal ganglion cell (RGC) layer.The apoptosis rate of RGCs was (43.4±5.0)% in the DR model group and (30.0±5.1)% in the capsaicin pretreated group,showing a significant difference (t =5.930,P<0.01).Compared with the DR model group and capsaicin pretreated group,the apoptosis rates of the DR control group (12.4±9.9) % and the capsaicin control group (17.6-±6.1) % were significantly lower (t =8.800,t =4.925,P<0.01).The apoptosis rate of the plain control group was (16.2±6.9)%,exhibiting significant differences in comparison with the DR control group and capsaicin control group (t =-0.989,t =0.951,P>0.05).The specific activity of caspase-3 was (2.19±0.86) in the DR model group and (1.96±0.56) in the capsaicin pretreated group,presenting a significant difference (t =-0.515,P<0.05).Those of the DR control group and capsaicin control group were (1.47±0.14) and (0.74±0.27),respectively,with considerable decline in comparison with the DR model group and capsaicin pretreated group (t=2.142,t=2.797,P<0.05).The retinal and serum CGRP levels were (424.4±44.2)and (148.8±39.1) ng/L,respectively,displaying significantly lower levels than (543.2±74.4) and (237.5±78.7) ng/L (t =3.070,2.359,P<0.05) from the capsaicin pretreated group.Conclusions Apoptosis of retinal ganglion cells occurs in the STZ-induced diabetic rats.Pretreatment of capsaicin reduces retinal cell apoptosis,which may be associated with an increase of CGRP in the retina.
6.Questionnaires on male sexual dysfunction and their clinical application.
Yang CAI ; Tao WANG ; Ji-Hong LIU
National Journal of Andrology 2014;20(9):840-845
With the increasing incidence and prevalence of male sexual dysfunction, andrologists are more and more in need of accurate and efficient tools to assess therapeutic efficacy and patients' satisfaction and to help patients achieve satisfactory treatment results. This article summarizes some of the most commonly used questionnaires for the diagnosis and assessment of the treatment of male sexual dysfunction, including International Index of Erectile Function (IIEF), Erection Hardness Score (EHS), Quality of Erection Questionnaire (QEQ), Erectile Dysfunction Inventory of Treatment Satisfaction (EDITS), Treatment Satisfaction Scale (TSS), Self-Esteem and Relationship (SEAR), Premature Ejaculation Profile (PEP), Premature Ejaculation Diagnostic Tool (PEDT), Index of Premature Ejaculation (IPE), Arabic Index of Premature Ejaculation (AIPE), Aging Male Symptoms Scale (AMS), Androgen Deficiency in the Aging Male (ADAM), and Symptomatic Inventory for Screening Late-Onset Hypogonadism in Males (SILOH), and presents an overview on their clinical application.
Erectile Dysfunction
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Humans
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Male
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Surveys and Questionnaires
7.Analysis of application and utilization of anti-tumor drugs and its assistant patent Chinese drugs.
Rui YANG ; Ji-hong GUO ; Yang JIANG
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(12):946-949
Antineoplastic Agents
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administration & dosage
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Costs and Cost Analysis
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Drug Prescriptions
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statistics & numerical data
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Drugs, Chinese Herbal
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administration & dosage
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Humans
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Materia Medica
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Neoplasms
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drug therapy
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Nonprescription Drugs
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therapeutic use
8.Clinical experiences of the pericranial and subcaleal fascial flap.
Ji Young SONG ; Won Yong YANG ; Sung Pyo HONG
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(5):783-790
No abstract available.
10.Determination of 4-Hydroxy-3-Methoxy Benzoic Acid by RP-HPLC and Inhibitory Effectof Phloroglucinol on Catecholamine-O-Methyl Transferase
Ruiling YANG ; Hong LIU ; Qi WANG ; Kefei JI ; Mengjiao SONG
Herald of Medicine 2014;(10):1303-1306
Objective To detect content of 4-hydroxy-3-methoxy benzoic acid by RP-HPLC and observe the inhibitory effect of phloroglucinol on catecholamine-O-methyl transferase (COMT). Methods This study used the principle of 3,4-dihydroxy benzoic acid transforming to 4-hydroxy-3-methoxy benzoic acid under COMT’ s catalytic action. COMT (20 μL) was extracted from mouse liver homogenate. In a reaction system,10 μL catecol (1í10-3 mol·L-1 ) and 10 μL phloroglucinol (5í10-3 , 1í10-3 and 2í10-4 mol·L-1 ,respectively) were added. Products were determined by RP-HPLC to analyze effects of 4-hydroxy-3-methoxy benzoic acid on COMT. Results Phloroglucinol had inhibitory effect on COMT activity at concentrations of 5í10-3 mol·L-1 ,1í10-3 mol·L-1 and 2 í10-4 mol ·L-1 ,with the inhibition rate being 25. 3% ,17. 6% and 8. 9% ,respectively. Conclusion Phloroglucinol has an inhibitory effect on COMT activity,which is weaker than the effect of catechol of the same concentration.