2.Clinical Efficacy of Yiqi Huoxue Decoction in the Treatment of Children with Spleen and Kidney Deficiency Type Nephrotic Syndrome
Chunshui DA ; Hong GAO ; Hong ZHANG ; Ruiqi HAI ; Wenshan QIAO
Progress in Modern Biomedicine 2017;17(26):5087-5090
Objective:To study the clinical efficacy ofYiqi Huoxue Decoction in the treatment of children with spleen and kidney deficiency type nephrotic syndrome.Methods:A total of 80 children with spleen and kidney deficiency type nephrotic syndrome in our hospital from June 2014 to October 2016 were enrolled in this study.The subjects were divided into control group (n=40) and treatment group (n=40) according to the random number table method.The control group was treated with prednisone,and the treatment group was treated with prednisone combined with Yiqi Huoxue Decoction,the two groups were treated for 4 months.The clinical efficacy of the two groups were evaluate.The 24h proteinuria,total cholesterol (TC),plasma albumin (Alb),and renal function parameters including blood urea nitrogen (BUN),serum creatinine (Scr),and serum creatinine clearance (Ccr) of the two groups before and after treatment were compared.Results:The total effective rate of the treatment group was 95.00%,which was significantly higher than 67.50% of the control group,the difference was statistically significant (P<0.05).The 24h proteinuria,TC,BUN and Scr of the two groups after treatment were significantly lower than before treatment,the Alb,Ccr of the two groups were significantly higher than before treatment,and above indexes in treatment group were better than the control group,the differences were statistically significant (P<0.05).Conclusion:Yiqi Huoxue Decoction have good clinical efficacy in the treatment of children with spleen and kidney deficiency type nephrotic syndrome,which can obviously improve children renal function,and is worthy of clinical application.
5.Prokaryotic soluble expression, purification and function study of LEDGF/p75 protein.
Da-Wei ZHANG ; Hong-Qiu HE ; Shun-Xing GUO
Acta Pharmaceutica Sinica 2014;49(8):1200-1207
HIV-1 integrase (IN) is a key enzyme for the viral replication. The protein-protein interaction (PPI) between HIV-1 IN and a cellular cofactor lens epithelium-derived growth factor (LEDGF/p75) is a validated target for anti-HIV drug discovery. In order to build the platform for screening inhibitor against PPI between IN and LEDGF/p75, the vector containing the LEDGF/p75 protein cDNA was constructed and expressed in Escherichia coli and the function of the LEDGF/p75 protein was assayed. The LGDGF/p75 encoding gene optimized according to the preference codon usage of E. coli, was synthesized and cloned into the expression vector pGEX-4T-1 to form a recombined plasmid, then transformed into host cell E. coli BL21 (DE3). The recombined clones were identified and confirmed by BamH I/Sal I digestion and sequencing, the successfully recombined plasmid in the host cell was induced by IPTG and the condition of the expression was optimized. The expressed protein was purified by the Ni2+ affinity chromatography column and SDS-PAGE was used to analyze the molecular weight and specificity. In addition, ELISA assay was used to analyze the function of the recombinant protein. The recombinant LGDGF/p75 was soluble, and expressed highly and stably in E. coli. The protein was proved to enhance HIV-1 IN strand transfer activity in vitro by ELISA. It will be helpful to build the platform of screening inhibitors against PPI between IN and LEDGF/p75.
Cloning, Molecular
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Escherichia coli
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metabolism
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HIV Integrase
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metabolism
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HIV-1
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physiology
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Humans
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Intercellular Signaling Peptides and Proteins
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biosynthesis
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Protein Binding
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Virus Replication
6.Expression of Heat-Shock Protein 70 in Kidney of Acute Injury Rats Caused by Gentamicin
hong-yan, ZHANG ; ling-di, SUN ; da-ya, ZHAO
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To illustrate the expressional distribution of heat shock protein 70(HSP 70) in the kidney of acute renal injury rats caused by gentamicin.Methods One hundred and fifty rats were randomly divided into 3 groups: group A (normal group), group B [80 mg/(kg?d) gentamicin to be injected], group C [160 mg/(kg?d) gentamicin to be given]. The variations of renal pathology were observed at different time phasess by light scope and electromicroscope. Simultaneously, the expression of HSP 70 in kidney was evaluated by immunohistochemistry.Results HSP 70 distributed in epithelial cells of renal tubular in acute injury rats. The expression of HSP 70 increased markedly from the 6th hour after injection, peaked at the 12th hour and lasted for 48 hours. The expression of HSP 70 in group C was higher than that in group B(P
7. Studies on chemical constituents in Guizhi Fuling Capsule (VII)
Chinese Traditional and Herbal Drugs 2020;51(16):4113-4116
Objective To study the chemical constituents from the n-butanol extract of Guizhi Fuling Capsule. Methods The chemical constituents were isolated and purified by multiple chromatographic methods. Their structures were identified on the basis of the spectral data and physicochemical properties. Results Five compounds were identified as 3'-O-galloylsucrose (1), 4'-O-galloylsucrose (2), 6'-O-galloylsucrose (3), 1'-O-galloylsucrose (4), and 1,2,3,4,6-pentagalloylglucose (5). Conclusion Compounds 1-5 are isolated from Guizhi Fuling Capsule for the first time, and compound 1 is a new compound. Compounds 1-5 inhibited calcium influx in uterine smooth muscle cells of primary mice.
8.CD34+ cell and interleukin 5 and eosinophil in the peripheral blood of allergic rhinitis patients.
Hui ZHANG ; Da-liang ZHANG ; Hong-ping ZHANG ; Yingjie CHEN ; Chenghao GUO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(7):541-542
Adolescent
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Adult
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Antigens, CD34
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blood
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Case-Control Studies
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Eosinophils
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Female
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Humans
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Interleukin-5
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blood
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Leukocyte Count
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Male
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Middle Aged
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Rhinitis, Allergic, Perennial
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blood
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Young Adult
9.Transperitoneal laparoscopic pyelolithotomy(report of 19 cases)
Li-Rong YUAN ; Da-Hong ZHANG ; Lei RUN ; Chang-Fu WEI ; Li-Xin ZHANG ;
Chinese Journal of Urology 2001;0(07):-
Objective To evaluate the feasibility and initial experience of laparoscopic pyelolithoto- my within renal sinus.Methods A total of 19 patients(14 men and 5 women)of nephrolithiasis on 21 sides underwent laparoscopic pyelolithotomy within renal sinus.Of them,9 cases with other upper urinary tract diseases were treated by laparoseopic pyelolithotomy at the same time.The patient age ranged from 16 to 67 years(mean,41 years).The calculi were 1.2 to 3.5 cm in diameter,and were found on the right side in 11 cases,left side in 6,and both sides in 2.Five cases concomitantly had ureteral calculi;of them,1 had bi- lateral ureter calculi with ipsilateral steinstrasse,2 had pyelic polyp(1 with giant hydronephrosis after open ureterolithotomy),and 1 had giant bydronephrosis with pyelolithiasis during pregnancy with double-J tube drainage.Twelve cases(13 sides)had experienced unsuccessful extracorporeal shock wave lithotripsy,and 1 failed with minimally invasive percutaneous nephrolithotomy.Results The procedure was successful in all the 19 cases.The operative time ranged from 75 to 240 rain(mean,115 rain).The intraoperative blood loss ranged from 30 to 100ml(mean,50 ml).One case experienced 5-d urine leakage,and was spontaneously cured.The hospital stay was 5-9 d(mean,6 d).Double-J tube drainage was 4-6 weeks.During the follow- up(3-36 months),KUB and IVU showed no upper urinary tract stricture and no residual stones except for 1 stone of 0.7 cm in the renal lower calyx in 1 case.The kidney function was improved in all cases.Con- clusions Laparoscopic pyelolithotomy is new and minimally invasive alternative approach for renal calculi. With this procedure the urinary tract complications can be treated concomitantly,thus it can partially replace open surgery.
10.Clinicopathologic study of pulmonary epithelioid hemangioendothelioma in thoracoscopic biopsy.
Hong-xia LI ; Zhi-hong ZHANG ; Da-li CHEN ; Xiao LI ; Wei-ming ZHANG ; Guo-xin SONG ; Qin-he FAN
Chinese Journal of Pathology 2012;41(3):197-198
Adenocarcinoma
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metabolism
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pathology
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Adult
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Antigens, CD34
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metabolism
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Biopsy
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Diagnosis, Differential
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Follow-Up Studies
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Hemangioendothelioma, Epithelioid
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diagnostic imaging
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metabolism
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pathology
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surgery
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Humans
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Lung
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metabolism
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pathology
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Lung Neoplasms
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diagnostic imaging
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metabolism
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pathology
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surgery
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Male
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Mesothelioma
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metabolism
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pathology
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Middle Aged
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Multimodal Imaging
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Platelet Endothelial Cell Adhesion Molecule-1
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metabolism
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Positron-Emission Tomography
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Thoracoscopy
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Tomography, X-Ray Computed
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Tuberculosis, Pulmonary
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metabolism
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pathology