This study is to establish the gastric cold model of rats. After gastric feeding with cold water for 5 weeks and extra iced water bath in the last 2 weeks, model group show distinct physical sign of gastric cold syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group(treated with Fanzuojin Wan) show mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than blank group. The difference with significance among model group, treatment group and blank group reveals the success of the establishment of gastric cold syndrome.
Animals ; Cold Temperature ; Disease Models, Animal ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach ; chemistry ; metabolism ; pathology ; physiopathology ; Stomach Diseases ; metabolism ; pathology ; physiopathology

This study is to establish the gastric hot model of rats. After gastric feeding with ethanol solution for 3 weeks and feeding with extra capsaicin and ethanol solution for another 2 weeks, model group show distinct physical sign of gastric hot syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group (treat with Zuojin Wan) shows mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than the blank group. The obvious difference among model group, treatment group and blank group reveals the success of the establishment of gastric hot model.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Eating ; Female ; Humans ; Male ; Rats ; Rats, Wistar ; Stomach Diseases ; drug therapy ; pathology ; physiopathology

Animals ; Avoidance Learning ; physiology ; Cues ; Extinction, Psychological ; physiology ; Fear ; physiology ; Male ; Mental Recall ; physiology ; Rats ; Sleep Deprivation ; physiopathology ; Sleep, REM ; physiology

Objective To evaluate the surgical effects of laparoscopic pancreaticojejunostomy in pigs and investigate the learning curve. Methods A total of 40 porcine cases of laparoscopic pancreaticojejunostomy were performed by a single surgical team from September 2012 to February 2013.According to the operation sequence, pigs were divided into 4 groups with 10 in each group.For analyzing the efficacy of surgery in different stages, the operation time and efficacy of anastomoses were compared between these groups. Results The operation was finished successfully in all the 40 cases.There were statistical significances in operation time among the four groups.The group Ⅳ had significant shorter time (112.7 ±8.3) min than those in the group Ⅰ [(145.1 ± 16.6) min, q=9.154, P<0.05], the groupⅡ[(133.0 ±8.7) min, q=5.735, P<0.05], and the group Ⅲ[(137.2 ±9.0) min, q=6.922, P<0.05], respectively.There was no difference between the group Ⅱ and the group Ⅰ in operation time (q=3.419, P>0.05), so as to other groups.The results of anastomoses among the four groups were not significantly different (χ2 =0.586,P=0.900). Conclusions Laparoscopic pancreaticojejunostomy in pigs is feasible.The learning curve of the operation is about 30 cases.

Objective To investigate the clinic value of enhanced CT-guided percutaneously transthoracic lung biopsy. Methods A total of 423 patients who underwent CT-guided percutaneously transthoracic lung biopsy were retrospectively analyzed. The percentage of specimen are satisfactory,the rate of sample with pathological findings,the number of cases with complications,the average goes of unsuccessful biopsy and the times of changing puncture point were compared between the enhanced CT group and the plain CT group. Results Compare parameters in patients whose procedure were guided with the enhanced CT group and with plain CT group, the average goes of unsuccessful biopsy, the times of changing puncture point in patients, the percentage of specimen that were satisfactory, the rate of sample with positive pathological findings and the rate that complicated with pneumothorax or hemorrhage show statistical significance.(3.08±1.05 vs 3.75±1.34, 8.52%vs 18.54%,90.13%vs 73.60%, 76.62%vs 63.36%, 15.51%vs 27.53%, 7.76%vs 14.04%, P<0.05). Conclusion In patient whose biopsys were guided by enhanced CT, the percentage of samples that are satisfactory is higher, the incidence of complication is fewer and the sample with pathological finding is higher compared to those in patients whose biopsys were guided by plain CT. Therefore, enhanced CT-guided percutaneous transthoracic biopsy is more valuable than plane CT.

Objective To analyze the risk factors of intracranial infection after neurosurgery and the effect of vancomycin.Methods 28 cases of craniotomy in this study, The First Hospital of Ningbo City from January 2011 to July 2014 during the years after the occurrence of intracranial infection patients as the research object, a retrospective analysis of the age, gender, emergency, basic diseases, mastoid airroom, ventricle drainage, posterior fossa surgery, reoperation and operation time (more than 4h).The clinical data, summarize the risk factors of postoperative intracranial infection in department of neurosurgery and to observe the clinical effect of vancomycin in the treatment of such patients.Result The high risk factors of intracranial infection include: Open mastoid gas room during operation, ireoperation, ventricular drainage and operative time (more than 4h).In 28 cases with intracranial infection after craniotomy, the total effective rate of vancomycin treatment was 100.00%.Conclusion The high risk factors of intracranial infection after operation include open surgery, mastoid room, reoperation, ventricular drainage and longer operation time.Vancomycin is an effective drug in the treatment of intracranial infection in the department of neurosurgery.

Objective To explore the clinical prognostic value of rapid detection for plasma NT-proBNP levels on admission in patients with acute myocardial infarction.Methods 56 patients with AMI were measured plasma NT-proBNP imediately in hospital,and then they were divided into A,B and C group according to NT-proBNP levels (A group:＜ 500ng/L,B group:500-2 000ng/L,C group:＞ 2 000ng/L).The incidence of major adverse cardiac events (MACE including congestive heart failure,malignant arrhythmia,cardiogenic shock and sudden cardiac death) in subjects were observed respectively during hospitalisation and 30 days.Results The three group subjects with different NT-proBNP levels presented different incidence of MACE(A group:1,0;B group:3,1;C group:8,6) at duration of hospital stay,30days (x2 =6.705,P =0.035 ; x2 =7.957,P =0.008).With the NT-proBNP levels rising in AMI paitents,the inciedence of MACE increased.The incidence of MACE in A,B and C group were 6.6％,18.18％ and 73.69％ respectively.Conclusion In AMI patients,plasma NT-proBNP levels could predict early MACE incidence,which has an important value to evaluate the early clinical prognosis.

OBJECTIVE:To evaluate the situation and the tendency of antihypertensive use in guangdong province.METHODS:The sales volume,defined daily use(DDDs)and daily expense of antihypertensive from2002to2004in guangdong province were investigated and analyzed.RESULTS:The sales volume of antihypertensive from2002to2004had been in?creasing.Calcium antagonist dominated over all other antihypertensives in respect of sales volume and DDDs.CONCLUSION:Calcium antagonist dominate in the antihypertensive market in guangdong province.

AIM: To investigate the changes in nuclear calcium content and permeability of nuclear pore complex in rat myocardium during ischemia reperfusion injury.METHODS: The rat model of myocardial ischemia reperfusion injury was established.Myocardial nuclei were purified using sucrose density gradient centrifugation.The nuclear calcium content was measured by atomic absorption spectrophotometer.The permeability of nuclear pore complex was assessed through measuring the amount of calmodulin conjugated Alexa Fluo~(TM) 488 as fluorescent probes transported across nuclear membrane with spectrofluorometer.RESULTS: The nuclear calcium content at 15,30,60,120 and 180 min reperfusion following 30 min sustained ischemia increased 1.31-,1.55-,1.73-,1.94-and 2.14-fold,respectively,as compared with sham-operation group.The permeability of nuclear pore complex at 15 min reperfusion following 30 min sustained ischemia showed no difference from sham-operation group,but it only increased 1.31-,1.38-,1.40-,and 1.48-fold at 30,60,120 and 180 min reperfusion following 30 min sustained ischemia compared with sham-operation group.CONCLUSION: The nuclear calcium content during myocardial ischemia reperfusion injury increases earlier than the permeability of nuclear pore complex does.The increase in the permeability of nuclear pore complex may result in adaptive regulatory effects on nuclear calcium overload to a certain extent during myocardial ischemia reperfusion injury.

Child ; Child, Preschool ; Complement C3 ; Disease Progression ; Female ; Humans ; Intercellular Signaling Peptides and Proteins ; blood ; Lipid Metabolism ; Male ; Obesity ; blood