In the present study, forced immobilization induced-pul-monary fat embolism as an experimental model was developed for the first time in rabbits.The area of emboli was calculated by an electronic caculator.At the same time, the activities of lipoprotein lipase in post-heparin plasma (PHP-LPL) and the concentrations of free fatty acids(FFA)were measured,The determination of plasma catecholamines, the analysis of blood gas, the observation of pulmonary morphology (including ultrastructure)were also carried out,It was found that forced immobilization for 5h could induced pulmonary embolism in rabbits, accompanied by the elevation of PHP-LPL and FFA in pulmonary circulation, hypocapnia, and the tendency of surfactant insufficiency.The results suggest that the disorder of homeostasis caused by stress is strong enough to induce pulmonary embolism without the participation of fracture

Objective To study the effect of atorvastatin on lipid profile modification and brachial artery endothelial function in hypercholestrolemic patients.Methods Totally 128 hypercholestrolemic patients were randomized to receive atorvastatin(10 mg/d,qn,n=64) or simvastatin as control(20 mg/d,qn,n=64).Blood lipid levels were determined prior to lipid lowering treatment and at the end of 4-and 8-week treatment respectively.The changes of brachial artery internal diameter under reactive hyperemia situation or after taking nitroglycerin(500 ?g) were respectively evaluated by B-mode ultrasonography.Results The effects of atorvastatin in lowering serum total cholesterol(TC) and low density lipoprotein cholesterol(LDL-C) and triglyceride(TG) levels were the same as simvastatin.The changes of brachial artery internal diameter under reactive hyperemia situation and after taking nitroglycerin(500 ?g) in hypercholestrolemic patients were significantly increased after treatment.Conclusion Atorvastatin could decrease the serum level of TC,LDL-C and TG and improve the brachial artery endothelial function,and its effects were similar to simvastatin.

Wogonoside,wogonin,baicalin and baicalein are major chemical constituents of S.baicalensis.Baicalin,baicalein and wogonin have been reported previously to exert anti-cancer effects.The present study compared the anti-cancer effects of the four flavonoids individually towards four human cancer cell lines after 24-and 48-hour treatment based on cell viability assay.As a result,wogonin inhibited the growth of the four cell lines.The IC50 values after 48 hours of incubation were 97.9 μM for A549 lung adenocarcinoma cells,while 15.3 μM for HeLa cervical carcinoma cells,147 μM for HepG2 hepatocellular carcinoma cells and 104 μM for MCF-7 breast adenocarcinoma cells.In contrast,wogonoside,the glycoside of wogonin,showed no inhibitory effect against any one of the four cell lines.Baicalein inhibited the growth of HeLa cells,while baicalin inhibited the growth of HepG2 cells,both with higher IC50 values and less potent than wogonin.These findings established wogonin as the most active anti-cancer agent among the four major flavonoids of S.baicalensis.Since baicalin,the most abundant flavonoid in the herb,was found to enhance the growth of MCF-7 cells.Clinical applications of ingredients from S.baicalensis to the treatment of cancer should be carried out with purified compounds.

Objective: Depression is prevalent in patients with diabet es It is associated with poor glycemic control and is linked to an increased r i sk for diabetic complications In this study, we assessed the efficacy of Parox e tine for depression in patients with diabetes Methods: 48 pati e nts with diabetes (type 1, n=7; type 2,n=41) and major depressive disorder enter ed an 8-week randomized placebo-controlled trial Patients were given daily dos e s of Paroxetine (up to 40 mg/day) The Beck Depression Inventory (BDI) and Hami l ton Rating Scale for Depression (HAMD) were used to measure the severity of depr ession and to determine the percentage of patients who achieved substantial impr ovement or complete remission GHb levels were obtained to monitor glycemic con t rol Results: Reduction in depression symptoms was significantl y greater in patients treated with Paroxetine compared with those receiving place bo (BDI-141 vs -89, P

The management of hospital medical periodicals is now facing many realistic problems such as management system of editorial department, ability of editorial team, quality of articles and outside living environment. Co-publishing periodicals with pharmaceutical enterprises, with foreign publications and innovating publishing model of periodicals have been adopted by some domestic hospitals. By studying cases of periodical management innovation, and summing up the latest domestic point of view on medical periodical management, this paper held the view that the hospital medical periodicals management should be grouped into the framework of hospital scientific research management. Basically, the hospital should focus on solving the problem of management system in priority;developing in a way of specialization, collectivization and brand-orientation;establishing of a high-level editorial team;strengthening international communication and publishing periodicals according to international rules.

OBJECTIVE:To study the release characteristics of Evening primrose oil microspheres in vitro. METHODS:UV spectrophotometry was adopted to determine the accumulated release of microspheres under different media,and the drug release behaviors in vitro were fitted using zero-order,first-order and Higuchi equations. RESULTS:The accumulated release of microspheres in the gastrointestinal liquid (within 6 h) was up to(92.84?0.35)%;under the media of artificial gastric juice,the release of drug conformed to Higuchi equation:Q=5.941t1/2—11.083(r=0.991 7); under the media of artificial intestinal juice,the release of drug was in line with first-rate equation:ln(100—Q)=—0.487 4t+4.588(r=0.996 4). CONCLUSION:This method is simple,accurate and reproducible,and it can be used for the determination of the release rate of the Evening primrose oil microspheres.

Transforming growth factor-?(TGF-?) is a multifunctional growth factor. It plays a very important role in the growth, dif ferentiation, migration, apoptosis of cells and production of extracellular matr ix throughout many signaling pathways. MAPK cascade is one of those signaling pa thways. TGF-? can activate MAPKs and fulfill its multiple regulation on a varie ty of cells.

Patent competition has become one of the key issues of global competitions in economy,science and technology. In hospitals, the patent management strategy provides guidance for and impels knowledge innovation. However, hospital management is quite weak in China, which may have been caused by various reasons, such as the over enthusiasm for published paper and the technological achievement contrasted with negligence in patent application and exploitation. In the paper, countermeasures are proposed,which include the whole process management during the scientific research, setting up detail targets and standard procedures for patent management, training patent management staff, et al.

Abundant evidences indicate a role of GABAergic system in the pathogenesis of neuropsychiatric dis-orders. GABAA receptors modulators have been long used in clinical. But the unwanted side-effect and risk of drug dependence have limited their use. With the increase understanding of the role of individual GABAA recep-tor subtypes in the pathology of these diseases, the subtype-selective GABAA receptors modulators and the multi-compound therapy may serve as potential therapeutic strategies. Here we review the researches which revealed the involvement of GABAA receptors in anxiety, depression and schizophrenia, and the research for new development in subtype-selective GABAA receptor modulators.

Objective:To explore the effect of costunolide on sensitivity of chronic myeloid leukemia cell line K562/ADR to doxorubicin and its mechanism.Methods:K562/ADR cells in the logarithmic phase were used, and the cells were treated with different concentrations of costunolide, doxorubicin or costunolide combined with doxorubicin for 72 h. The cell proliferation was detected by CCK-8 method, the cell proliferation rate was calculated, and the half inhibitory concentration (IC 50) of the two drugs was obtained. The cells were treated with 10 μmol/L costunolide, 10 μmol/L doxorubicin or costunolide combined with doxorubicin for 48 h, the apoptotic rate was detected by flow cytometry, and the expression level of p38-MAPK pathway related proteins was detected by Western blot. Results:The cell proliferation rate in the costunolide combined with doxorubicin group was lower than that in the corresponding concentration of the two drugs alone groups, and the differences were statistically significant both ( P < 0.05). The IC 50 of doxorubicin for K562/ADR cells was (13.50±0.86) μmol/L, and costunolide was (7.30±0.55) μmol/L ( t = 7.044, P = 0.002). The results of flow cytometry showed that the apoptosis rate of K562/ADR cells in the 10 μmol/L costunolide combined with 10 μmol/L doxorubicin group was higher than that of the blank control group, costunolide alone group and doxorubicin alone group, and the differences were statistically significant [(19.68±3.21)% vs. (2.96±0.87)%, (9.34±2.89)%, (9.18±2.13)%, all P<0.01]. Compared with the 10 μmol/L costunolide alone group and the 10 μmol/L doxorubicin alone group, the expression of the apoptosis inhibitor protein bcl-2 in the two-drug combination group was down-regulated, and the expressions of bad, p-p38, cleaved-caspase-3 and cleaved-PARP proteins were up-regulated. Conclusion:Costunolide can enhance the inhibitory effect of doxorubicin on the proliferation of K562/ADR cells and promote doxorubicin-induced apoptosis, which may reverse the drug resistance of K562/ADR cells by regulating the p38-MAPK pathway.