3.Research Progress on Insect-resistant Genes From Plants
Hong-Mei CHEN ; Kun-Zhi LI ; Li-Mei CHEN ;
China Biotechnology 2006;0(11):-
Plant insect-resistantgenetic engineering hasopened a new way to prevent and cure against the insect pest incrop and wood productions. With the progresses instudies, many insect-resistantgeneswere cloned and characterized. The classification of most insect-resistantgenes from plant, the functionalmechanism and the applications of theplantinsect-resistantgenes, the problems and prospectsforusing the plant insect-resistant genes in genetic engineering was described.
7.Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gram-negative Bacteria
Hong-Juan NIAN ; Li-Mei CHEN ; Kun-Zhi LI ;
China Biotechnology 2006;0(12):-
With development of wide-host-range vector systems,Tn5 transposon and its derivative vectors have been widely applied to genetic research of gram-negative bacteria.The applications of Tn5 transposon mutagenesis technology to genetic researches of bacteria were briefly discussed,including researches on biological control mechanisms of biocontrol bacteria,identification of bacterial essential genes,discovering virulence genes of bacterial pathogens,characterization of metabolism regulatory genes and genetic improvements of bacteria.
8.Application of combination transfer pump to continuous blood purification
Hongjun YANG ; Zhi CHEN ; Lidan GAO ; Hong ZHANG ; Lu KANG
Chinese Medical Equipment Journal 1989;0(04):-
Objective To evaluate the effect of combination transfer pump and single blood pump to 32 cases of renal failure patient who need blood purification therapy.Methods The combination transfer pump and single blood pump were used.According to the clinical requirement,the parameters of transfer pump were set such as therapy time,replacement liquid flow rate,dialysate flow rate,temperature of warmer and filtrate flow rate,etc.Results 32 patients with varying degrees of illness have been alleviated.Patients' acute left heart failure,water and electrolyte turbulences,acid intoxication and azotemia caused by liquid retention were corrected,which ensured a role for further treatment.Conclusion It is the functions of adjustable time and transfusion volume that are used in the combination transfer pump,the task of which is accomplished by several transfer pumps together.This method is easy to implement and can meet the clinical requirement largely without special CBP instrument.
9.Diffusion tensor imaging(DTI) and its application in visual development and amblyopia research
Junjie, ZHI ; Yongxin, XING ; Hong, YAN ; Jun, CHEN
Chinese Ophthalmic Research 2010;28(3):281-284
Diffusion tensor imaging(DTI) is an efficient and noninvasive tool to investigate the characteristics and functions of white matter fiber tracts,and it can be used to study the relationship between the structure and function in the nervous system,the plasticity and damage repair of visual development,abnormalities of the white matters that relate to the visual pathways.Recently,the quantification of white matter fiber tracts from the lateral geniculate bodies to the occipital lobe visual cortex using DTI offers morphological information for the function and structure of the white matters during visual development.Some studies attempt to compare the DTI outcomes and tractography between amblyopic children and normal children so as to better display the variations of optic radiation in children with amblyopia,and to evaluate the relationship between structure and function of the white matters.DTI becomes an important supplementary research approach in these fields.This paper reviews the application of DTI in visual development and amblyopic eyes.
10.Detection of Legionella Pneumophila in Low Respiratory Tract Infection in Children Less Than 5 Years Old
guo-hong, ZHU ; ying-shuo, WANG ; zhi-min, CHEN
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To study the present situation of legionella pneumophila(Lp) infection of low respiratory tract infection in children less than 5 years old.Methods Three hundred hospitalized patients with the diagnosis of low respiratory tract infection were enrolled in the study,191 males,109 females,aged 27 d to 5 years old,course from 1 d to 6 months.Serum antibody(IgM,IgG) of Lp serogroup 1-7 was detected by enzyme-linked immunosorbent assay(ELISA).Results In the patients,17 cases were positive,and the positive rate was 5.67%.Of the 17 children,the males(n=11) and females(n=6) did not significantly influence the positive rate(5.76% vs 5.50%).Four of them were between 27 days and 1 year old;7 were 1 to 3 years old,6 were 3 to 5 years old.The positive rates were significantly different among the 3 groups(2.53% vs 7.37% vs 12.77%) and tendentially increased following the increase of age(?2=7.8164 P