Objective To investigate health behavior level and self-efficacy in patients underwent coronary artery stent implantation,and to discuss the relationship between health behavior and self-efficacy and to offer proof to the intervention strategy of health behavior.Methods HPLP Ⅱ,self-efficacy questionnaire were used as main tools.106 patients underwent coronary artery stent implantation were investigated.Statistics analyses were performed using the Statistical Package for Social Science(SPSS)13.0.Results (1)The score of total HPLPⅡwas 130.08±21.25,the range of that was 84～180.The health behavior level of47.2%patients was general or worse,and that of 52.8%patients was good or better.(2)The score of self-efficacy was 71.37±16.32,the range of thatwas 38～110.(3)There were significant positive relationship in the scores of total HPLP Ⅱ and the six elements,and self-efficacy.The correlation coefficient was 0.389～0.916(P＜0.01).Conclusion The health behavior level of patients underwent coronary artery stent implantation was not in an ideal state.Self-efficacy has a positive effect on health behavior.The importance motivating factor should be attached to nursing practice and education by medical staff,and medical staff should establish and supervise to carry out corresponding intervention measure.

Objective To investigate the pathological changes of ultrastructure of mucosa in various bronchial segments from type 2 diabetic patients. Methods Sixteen cases of type 2 diabetic patients were selected,2-3 pieces of bronchial mucosa and submucosal tissue of the lesion were taken from various bronchi during bronchoscopy and these samples were observed under a transmission electron microscope. Results The basal lamina of bronchial capillary were diffusely thickened and mostly showed onion-skin like change,protein deposited around and mixed with basal menbrance;irregular highly electron dense materials were found to deposite around capillary,capillary lumen became narrow or even collapsed,neutrophilic leucocyte marginated in lumen and adhered with endothelium;protein deposited in the interstitial;endothelial cells and pericytes had dark cell changes.The cistern of rough endoplasmic reticulum dilated and vesicle formed.Conclusion Bronchial mucosa and its surrounding tissues show characteristic pathological changes of diabetes,bronchial is also the target organ of chronic diabetic damage.

Objective To investigate the mechanism of acupuncture in anti-depression. Methods 100 depressive patients were randomly divided into acupuncture group and Amitriptyline group, 50 cases in each group. They were respectively treated by acupuncture at Neiguan (PC6), Sanyinjiao (SP6), Baihui (DU20), Fengchi (GB20), and oral western meicine. The needles were manipulated once every 20 minutes and treatment was given once a day. HAMD score and levels of serum cell factors of two groups were observed. Results Serum levels of interleukin 1? (IL-1?), interleukin-6 (IL-6) interleukin-2 (IL-2) and tumor necorsis factor-? (TNF-?) in depressive subjects before treatment were significanly higher than that of nomal subjects (all P

[Objective]The clinical efficacy of endometriosis pelvic pain using traditional Chinese medicine in cycle treatment is discussed in the paper.[Methods] Forty patients of endometriosis diagnosed by chromatic colour type-B ultrasound or 1aparoscopy with typical dysmenorrhea were treated for three months in cycle therapy with traditional Chinese medicine based on dispersing liver,clearing away heat and activating blood circulation.The pain symptoms are evaluated before and after therapy per month.[Results]After three months treatment of traditional Chinese medicine,the diversified bellyache was significantly relieved and dysmenorrhea score was alleviated from 4.83?1.6 to 2.85?2.23(P

Objective To study the value of blood glucose changes and the severity of the disease in patients with the systemic inflammatory response syndrome(SIRS).Methods In 328 SIRS patients without diabetes,intravenous blood glucose was monitored before open intravenou channel and subsequently 3~8h after intervals.The blood glucose was reviewed if glucose was abnormal 24h later.Results The blood glucose was higher than normal in 287(87.5%)patients with SIRS,of which 44 cases were death(13.4%).The higher the blood glucose level was,the higher the mortality rate was.Conclusion Blood glucose of patients with SIRS was elevated.If the blood glucose was 3 times higher than the normal,patients with SIRS had a higher mortality rate.

Objective To investigate the effects of early pregnancy exposure to hazardous indoor air pollutants due to interior decoration and hyperthermia on neurobehavioral development of postnatal rats.Methods Twenty-two pregnant rats were randomly divided into 4 groups.Each group was placed in pollutants cabinet,hyperthermia cabinet,hyperthermia+pollutants cabinet and empty cabinet respectively for 2 h per day for 10 d since their pregnancy.The weight and neurobehavioral development of postnatal rats were observed at different time points.The number of apoptotic and dead neurons in the CA1 region of hippocampus was observed in postnatal rats.The expression of the NR1 subunit of NMDA receptor of hippocampal neurons was also detected.Results Indoor pollutants and hyperthermia could accelerate apoptosis and necrosis of hippocampal neurons in CA1 region of postnatal rats and increase the expression of NR1 subunit of NMDA receptor.Furthermore,hazardous indoor pollutants and hyperthermia could induce remarkable influences on postnatal rats’ neurobehavioral development,learning and memory capability.Indoor pollutants combined with hyperthermia aggravated these effects.Conclusion Exposure to environment of the hazardous indoor pollutants especially combined with hyperthermia during early pregnancy has remarkable influences on the nervous system of postnatal rats.

Objective To study the effects of Ginkgo biloba extract(EGb) on the volume of cerebral infarction and expression of caspase-3 in ischemic-reperfusion rats.Methods Setting up the brain ischemic and reperfusion model in rats by Nagasawa way.Both groups rats treated with small dose of EGb and large dose of EGb were injected intraperitoneally with EGb 50 mg/kg and 100 mg/kg at 30 min before the trial and 1 h after cerebral ischemic respectively.The infarction volume and caspase-3 expression were detected by TTC staining and immunohisto-chemistry.Then the results were compared with ischemic-reperfusion group.Results In groups treated with small dose of EGb and large dose of EGb,infarction volumes [(83.4?2.9)mm3,(37.0?2.5)mm3] were smaller obviously than that in the ischemic-reperfusion group [(138.8?6.1)mm3](all P

BACKGROUND:Self-assembling short-peptide has been shown to be a kind of hovel nanometer biomaterial.Following culture combined with chondrocytes,self-assembling short-peptide can promote eell marex secretion,cell division and proliferation.and eell phenotype maintenance.For this reason,it has become a candidate for all ideal cell scaffold used for cartilage tissue engineering.OBJECTIVE:To observe the biological characteristics of rabbit articular chondrocytes cultured in three-dimensional nanoscale self-assembling short.peptide KLD-12 hydrogel seaffold.DESIGN.TIME AND SETTING:An observational experiment was performed at the laboratory of Institute for Nanobiomedical Technology and Membrane Biology,Sichuan University between November 2007 and April 2008.MATERIALS:Nanoscale short-peptide KLD-12 with a sequence of Ac-KLDLKLDLKLDL-CONH2 was synthesized by Shanghai Biotech Company,China.METHODS:Chondrocytes were harvested from New Zealand rabbit articular cartilage by enzyme digestion.Following in vitro culture.chondrocytes of passage 3 were inoculated into 4 g/L self-assembling short-peptide KLD-12 solution at a density of 5x 109/L.Peptide gel formation was induced by phosphate buffered saline for three-dimensional culture.MAIN OUTCOME MEASURES:①Observation of chondrocyte morphology in the short-peptide hydrogel with inverted phase contrast microscope.②Detection of intracellular matrix secretion by toluidine blue staining and immunohistochemistry.③Measurement of mRNA expression levels of aggrccan,type Ⅱ collagen and type Ⅰ collagen in chondrocyte-seeded hydrogels by reverse transcription-polymerase chain reaction.RESULTS:①The viability of chondrocytes digested and isolated from rabbit articular cartilage was over 95%.④Chondrocytes cultured in three-dimensional short-peptide hydrogel maintained their round shape,proliferated actively,and aggregated in clusters.In addition.cartilage lacuna could be seen surrounding the clusters.③Both toluidine blue staining and type Ⅱ collagen immunohistochemistry results were positive.The mRNA expression levels of aggrecan and type Ⅱ collagen were increased with time.④Reverse transcription-polymerase chain reaction results revealed that after 3 weeks of in vitro culture in the short-peptide hydrogel,chondrocytes maintained the capability to secrete aggrecan and to express type Ⅱ collagen.CONCLUSION:Self-assembling short-peptide KLD-12 three-dimensional hydrogel Call well inainrain the normal cellular morphology,function and proliferative capability of chondrocytes

Objective To construct eukaryotic expressing vector of the mouse NMNAT1 (nicotinamide mononucleotide adenylyl-transferase) gene and examine its ability to express the NMNAT1 gene in Hela cells.Methods The full-length NMNAT1 cDNA sequence was amplified by PCR and cloned into the plasmid of T-vector and then to pcDNA3.1 construct.The recombinant plasmid pcDNA3.1-NMNAT1 was identified by DNA sequencing and then transfected with Lipofectamine2000 into Hela cells.The expression of NMNAT1 was detected by real time quantitative PCR (qPCR) and Western blot after 48 h transfection.Results The recombinant eukaryotic vector carrying NMNAT1 gene was constructed successfully in a match with database and this vector could up-regulate the expression of the NMNAT1 gene both in mRNA and protein levels in Hela cells.Conclusions The eukaryotic vector carrying NMNAT1 gene (pcDNA3.1-NMNAT1) enhances the expression of NMNAT1 gene.

Objective:To investigate the influence of Mannan Peptide combined with 5-Fu on the proliferation and apoptosis of HepG2 cells and to explore the related mechanism.Methods:HepG2 cells were divided into four groups:the control group,5-Fu group,Mannan Peptide group and combination group.Cell proliferation was evaluated by MTT assay.Flow cytometry was used to examine the cell cycle,proliferation index(PI)and apoptosis ratio.The expression of Bcl-2 protein in each group was analyzed by immunocytochemical method.Results:The inhibitive effect of 5-Fu plus Mannan Peptide was significantly stronger than that of Mannan Peptide alone,in a time-and dose-dependent manner. Cells were treated with medicine for 48 hours.Compared with cells in the control group and 5-Fu group,the percentage of cells in G_0/G_1 stage and the apoptosis ratio in the combination group were increased(P<0.01).In immunocytochemical analysis,the combination of Mannan Peptide and 5-Fu could significantly decrease Bcl-2 protein level(P<0.01).Conclusion:The combination of Mannan Peptide and 5-Fu can synergistically suppress the proliferation and induce apoptosis of human hepatoma HepG2 cells.The mechanism might be associated with down-regulation of Bcl-2 protein expression.