Objective To clone and bioinformatically analyze the full-length sequence of F protein binding protein 1 (FBP1). Methods Yeast two-hybrid system was employed to obtain an unknown gene and named it as FBP1.The coding sequence of FBP1 was cloned using molecular biological techniques. Results The coding sequence of FBP1 was cloned successfully. Conclusion FBP1, a cellular protein binding to F protein of HCV, plays an important role in the interaction of virus protein and host cell protein.

AIM: Mesenchymal stem cells (MSCs) isolated from human umbilical cord blood can differentiate into hepatocyte-like cells under a suitable induction condition. The optimal condition is still unclear. This study investigated the feasibility of differentiation of MSCs isolated from human umbilical cord blood into hepatocyte-like cells with hepatocyte growth factor (HGF) and interleukin-6 (IL-6) in vitro to find a new cell source for live tissue engineering. METHODS: The experiment was performed from March to September 2007 at the Central Laboratory of First Hospital of Lanzhou University. ①Three cases of umbilical cord blood were collected from full-term pregnant women. Pregnant women had signed an informed consent. The experiment was approved by Hospital’s Ethical Committee. ②MSCs from umbilical cord blood were separated by density gradient centrifugation and adherent method. Cell surface molecule was measured by flow cytometry. Third passage of cells were divided into four groups and cultured in DMEM medium with HGF (HGF group), IL-6 (IL-6 group), HGF+IL-6 (HGF+IL-6 group) or no growth factor (control group). ③The characteristics of proliferation and growth of MSCs were studied by microscopy and methyl thiazolyl tetrazolium (MTT). The phenotypes of MSCs were identified by flow cytometry and immunohistochemical method. Albumin levels in culture supernatants were determined with enzyme-labeled immunosorbent assay (ELISA). RESULTS: ①Growth and division of adherent cells obtained from human umbilical cord blood were good. The shape of MSCs changed into triangle, polygonal or round on days 21-28 in induction groups. ②Positive staining reaction for alpha fetoprotein (AFP) on day 7, for CK18 on days 21, 28 after induction. Albumin production by induced MSCs increased in a time-dependent manner. The positive percents of every hepatocytic marker were higher in the HGF+IL-6 group than in the IL-6 group and HGF group (P

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OBJECTIVE:To prepare and appraise eucalyptus oil?-cyclodextrin inclusion compound,and to testify the feasibility of transforming the dosage form of eucalyptus oil by clathration techniques.METHODS:The physicochemical properties of inclusion compound was identified by thin-layer chromatography(TLC),infrared spectroscopy(IR),ultra-violet spectroscopy(UV)and gas chromatograph mass spectrometer(GC-MS),respectively.Meanwhile,the changes in constituents and clathration outcomes of eucalyptus oil before and after clathration were also investigated.RESULTS:The analytic result of TLC,IR and UV showed that stable inclusion compound has been formed from eucalyptus oil and?-cy?clodextrin.The result of GC-MS demonstrated that there was no significant change in the essential components and the percentage composition of eucalyptus oil before and after inclusion.CONCLUSION:Stable inclusion compound can be made from eucalyptus oil and?-cyclodextrin meanwhile without changes in main components and the percentage composition of eucalyptus oil.

Objective To describe the change of chloroform concentration in swimming pool water and to study the influence factors. Methods The swimming pool water samples were collected in 10 public swimming pools and were determined and evaluated according to the Sanitary Standard for Drinking Water(2001), GB/T 17220-1998, GB/T18204.9-2000, GB9667-1997. Results Chloroform concentration in 21 water samples was from lower than 0.006 to 0.184 mg/L, the median was 0.120 mg/L. In the present study, chloroform concentration was correlated positively with turbidity, CODMn, tetrachloride, the free chlorine residual and pH. A negative correlation was found between chloroform concentration and coliform group. Chloroform concentration among the different swimming pools exhibited statistical significance. Conclusion Chloroform concentration in swimming pool water was significantly higher than that in tap water in Jiading, Shanghai. Change of chloroform is different among swimming pools. The increase of chloroform may be related to the pollutants and chlorination in swimming pool water.

Carotid ultrasound examination is a very mature examination method, it is widely used in clinical diagnosis and health checkup. Carotid ultrasound examination plays an important role in the prediction of the occurrence of cardiovascular disease. However, there are still certain problems in the application of the examination in the domestic health checkup population, which mainly include that the method and the standard are not unified, and the reporting form is not standardized. Carotid ultrasound examination must be standardized as soon as possible.

Objective To investigate the treatment effect of fasudilon inflammatory bowel disease ( IBD) in rats. IBD was induced by using an enema of trinitro-benzene-sulfonic acid ( TNBS ) . Methods A total of 30 female SD rats were randomly divided into normal control group (0. 9% sodium chloride for induction and 0. 9% sodium chloride for treatment), model control group (TNBS for induction and 0. 9% sodium chloride for treatment), and fasudil group (TNBS for induction and fasudil 4. 5 mg·kg-1 for treatment). The disease activity index(DAI) was estimated 14 days later. The MPO activity, TNF-αcontent and IL-1β level in the colon were investigated, while Rho kinase expression was detected by Western blot. The pathological changes were detected by HE staining. Results The DAI of the normal control, model control, and fasudil groups were:(0. 00±0. 00),(7. 76±1. 32),and (3. 20±0. 98), respectively. MPO activity was (59. 32±9. 08),(96. 65±16. 57),and (69. 58±11. 40) U·g-1, respectively. TNF-α was (0. 15±0. 11),(0. 28±0. 22),and (0. 20±0. 62) ng·mL-1, respectively. IL-1β content was (0. 04±0. 01),(0. 08±0. 02),and (0. 06±0. 02) ng·mL-1, respectively. Rho kinase expression was 0. 713± 0. 170,1. 083±0. 210,and 0. 907±0. 260, respectively. Conclusion Fasudil excerts protective effects against IBD in rats by lowering the expression of Rho kinase and attenuating the release of inflammatory mediators, suggesting that Rho kinase could be a new therapeutic target for IBD.

Objective To evaluate the effects of dexmedetomidine on the oxidative stress responses during global cerebral ischemia-reperfusion (I/R) in rats.Methods Thirty-six male Sprague-Dawley rats,weighing 250-300 g,were randomly divided into 3 groups (n =12 each) using a random number table:sham operation group (group S),global cerebral I/R group (group I/R) and dexmedetomidine group (group D).Global cerebral ischemia was induced by occlusion of bilateral common carotid arteries combined with hypotension (MAP maintained at 35-45 mmHg).In group D,dexmedetomidine was infused at a rate of 3 μg · kg-1 · h-1until 2 h of reperfusion after a loading dose of dexmedetomidine 3 μg/kg was intravenously injected immediately after onset of reperfusion.The neurological deficit score (NDS) was assessed at 24 h of reperfusion,the rats were then sacrificed,and their brains were immediately removed for determination of cell apoptosis and levels of malondialdehyde (MDA),superoxide dismutase (SOD) and catalase (CAT).Apoptotic rate was calculated.Results Compared with group S,NDS,apoptotic rate and MDA level were significantly increased,and SOD and CAT levels were decreased in I/R and D groups.Compared with group I/R,NDS,apoptotic rate and MDA level were significantly decreased,and SOD and CAT levels were increased in group D.Conclusion Dexmedetomidine attenuates global cerebral I/R injury through inhibiting the oxidative stress responses.

Objective To investigate the effects of dexmedetomidine on global cerebral ischemia-reperfusion (I/R) injury in rats.Methods Fifty-four adult male SD rats weighing 200-250 g were randomly divided into 3 groups (n =18 each): shame operation group (group S),global cerebral I/R group (group I/R) and dexmedetomidine group (group D).Global cerebral I/R was produced by occlusion of bilateral common carotid arteries combined with hypotension (MAP maintained at 35-45 mm Hg).In group D dexmedetomidine 3 μg/kg was injected iv immediately after I/R,followed by infusion of dexmedetomidine at a rate of 3 μg· kg- 1 · h- 1 until 2 h of reperfusion.The neurological deficit score (NDS) was assessed (0 =normal,100 =brain death) at 6 h (T1),24 h (T2)and 72 h (T3) of reperfusion.Then six rats were sacrificed in each group and brain tissues were removed for microscopic examination of hippocampus CA1 region and determination of activity of myeloperoxidase (MPO),contents of TNF-α and IL-1β and expression of glial fibrillary acidic protein ( GFAP).Results Compared with group S,NDS,MPO activity and the contents of TNF-α and IL-1β at T1-3 were significantly increased,the expression of GFAP was up-regulated at T2,3 in groups I/R and D ( P ＜ 0.05 or 0.01).Compared with group I/R,NDS,MPO activity and TNF-α concent were significantly decreased at T1-3,IL-1β concent was decreased at T1,2,the expression of GFAP was down-regulated at T2,3 in group D (P ＜ 0.05 or 0.01 ).The pathologic changes were significantly attenuated in group D as compared with group I/R.Conclusion Dexmedetomidine can attenuate global cerebral I/R injury in rats,and the inhibition of inflammatory response may be involved in the mechanism.

Objective To apply EP7‐A2 document for evaluating the interference of hemolysis the total bilirubin detection in clinic .Methods The interferent was affirmed according to thepaired‐differencetest required by the EP7‐A2 document .The rela‐tion between interferent concentration and interference degree was analyzed by using the dose‐effect test .Results The paired‐difference test results showed that 5 .00 g/L hemoglobin had negative interference effect on two kinds of total bilirubin reagents . But the reagent kit A had little interference degree of hemolysis;The dose‐effect test results showed that hemoglobin produced the linear negative interference effect on the reagent kit A of total bilirubin detection .The linear equation of low value sample was Y=-0 .146X+14 .1 ,r=0 .964 ,which of middle value sample was Y = -0 .546X+92 .24 ,r=0 .947 and which of high value sample was Y= -1 .153X+307 .2 ,r=0 .979 .Conclusion Hemolysis has a negative interference effect on total bilirubin detection in clinic . The total bilirubin value could be corrected by using the hemoglobin concentration of sample;the EP7‐A2 document has certain ap‐plication value in the aspect of analyzing interference and evaluation .