1.Signal pathways in breast cancer stem cells and the targeted stem cell therapy.
Chinese Journal of Oncology 2010;32(12):881-885
Animals
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Breast Neoplasms
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metabolism
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pathology
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therapy
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Cell Proliferation
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Female
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Hedgehog Proteins
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metabolism
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Humans
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Neoplastic Stem Cells
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metabolism
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pathology
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Phosphatidylinositol 3-Kinases
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metabolism
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Receptors, CXCR4
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metabolism
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Receptors, Notch
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metabolism
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STAT Transcription Factors
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metabolism
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Signal Transduction
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Wnt Proteins
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metabolism
2.Protective effects of Lazaroid on early-stage diabetic retinopathy of rats
hong, WANG ; yuan-yuan, GONG ; kun, LIU ; xun, XU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(06):-
0.05).Amplitudes of OPs and b wave were significantly decreased after experiment in STZinduced diabetic group(P
4.A randomized controlled trial of the efficacy of combination of gemcitabine and carboplatin or paclitaxel and combination of paclitaxel and carboplatin in the treatment for advanced non-small cell lung cancer.
Qing GAO ; Fu-yuan WANG ; Hong YU
Chinese Journal of Oncology 2011;33(11):874-876
Adult
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Aged
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Aged, 80 and over
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Anemia
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chemically induced
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Antineoplastic Combined Chemotherapy Protocols
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adverse effects
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therapeutic use
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Carboplatin
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administration & dosage
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Carcinoma, Non-Small-Cell Lung
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drug therapy
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pathology
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Deoxycytidine
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administration & dosage
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analogs & derivatives
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Female
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Follow-Up Studies
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Humans
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Leukopenia
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chemically induced
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Lung Neoplasms
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drug therapy
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pathology
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Male
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Middle Aged
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Neoplasm Staging
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Paclitaxel
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administration & dosage
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Remission Induction
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Survival Rate
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Thrombocytopenia
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chemically induced
5.Effects of Light Pollution on Development of Myopia in Guinea Pigs
Hong WANG ; Kang ZHUANG ; Yuan TAO
Journal of Environment and Health 1989;0(06):-
green light(480 nm).
6.Selective of different surgical procedure in treatment of thoracic and lumbar spine tuberculosis with severe kyphosis and paraplegia
Hao WANG ; Julaiti TAYIERJIANG ; Hong YUAN
Orthopedic Journal of China 2006;0(11):-
[Objective]To evaluate the clinical results of the patients with severe kyphosis and paraplegia in differnt spine segment due to tuberculosis of thoracic and lumbar which had been treated by different surgical procedure. [Methods]There were a total of 23 patients, 16 male and 7 female in this stuady. The average age were 25 years, ranging from 12~56 years old. Tuberculosis lesion were located in different spine segment(T1~4)four cases, (T5~10)four cases, (T11~L2) nine cases,(L3~5) six cases, two vertebral bodies were involved in 8 patients and three vertebralbodies involved in 12 patients. Surgical procedure:four patients treated through modified anterior cervico-thoracic approach.Five patients treated through one stageposterior total vertebral osteotomy, other patients combined with anterior-posterior approach, according to the patient's surgical situation, twelve patients accepted anterior and posterior combined operation, two patients were treated by two stages.The period of bone grafting fusion, kyphosis deformity correction degree ,and neurological function recovery record were investigated postoperatively.[Results]All patients got solid fusion in a average of seven months. The kyphosis deformity was corrected 49? in average, which was maintained well within follow-up period. Patients neurological function deficiency achieved completely recovery, the longest time being within 10 months. All patients were cured thoroughly and there was no seroius complication.[Conclusion]Different surgical procedure should be selected, according to different spine segments due to tuberculosis of thoracic and lumbar vertebrae with the extent of severe kyphosis and paraplegia, it can achieve thoroughly debridement of tubereulosis, solid interbody fusion, good correction of deformity and forever stability of spine with anterior or posterior internal fixation.It could get successful rehablitation for patient’s neurological function and improve the patient’s life quality.
7.Effect of propofol on the expression of BMF-7 mRNA in heart daring ischemia-reperfusion in rats
Zhiguo YUAN ; Hong MA ; Junke WANG
Chinese Journal of Anesthesiology 1994;0(01):-
Objective To investigate the effect of propofol on the expression of bone morphogenetic protein-7 ( BMP-7 ) mRNA in heart during ischemia-reperfusion ( I/R) in rats. Methods Thirty Wistar rats weighing 280-300 g were randomly divided into three groups of 10 animals : (1) control group; (2) I/R group and (3) propofol group. In control group sham operation was performed. Chest was opened and closed. In I/R group the anterior descending branch of the left coronary artery was reversibly blocked with a 3-0 thread. Ischemia of the myocardium was confirmed by the color of apex turning from bright to dark red. The block was maintained for 10 min, then relieved for reperfusion. In propofol group propofol 1mg?kg-1?min-1 was continuously infused iv during 10 min of myocardial ischemia. The animals were saclificed at the end of 4h reperfusion. Specimen was obtained from apex of heart for determination of expression of BMP-7 mRNA using RT-PCR. Results The expression of BMP-7 mRNA was 1.14 ?0.08 in control group, significantly higher than that in I/R group (0.9 ? 0.05) and propofol group (1 .00 ? 0.08) (P
9.Apoptosis of human fetal glomerular endothelial cell induced by neutrophil proteinase 3 from ANCA positive children with SLE
Hong MA ; Cuiling WANG ; Yuan LIU
Chinese Journal of Rheumatology 2000;0(06):-
Objective To investigate whether the neutrophil serine proteinase 3 (PR 3),a specific autoantigen of C ANCA,could induce human fetal glomerular endothelial cell apoptosis in vitro .Methods PR 3 was isolated from polymorphonuclear leukocytes obtained from C ANCA positive children with SLE.The cultured glomerular endothelial cells from human fetal kidneys were treated with PR 3 and apoptosis was assessed by different methods including the propidium iodide staining,DNA fragmentation analysis,and morphology observation.Results Percentage of apoptotic cells significantly increased ( P
10.Effects of high glucose on number and activity of endothelial progenitor cells from peripheral blood
Hong YUAN ; Junzhu CHEN ; Xingxiang WANG
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
Objective To investigate the effect of high glucose on the number and proliferation, migration and adhesion of peripheral endothelial progenitor cells (EPCs). Methods Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin coated culture dishes. After 7 days of culture, several groups of attached cells were incubated with glucose in a series of concentrations (15, 25, 35, 45mmol/L) for different durations (6, 12, 24 and 48h). EPCs were characterized as adherent cells which were double positive for DiLDL uptake and lectin binding by direct fluorescent staining demonstrated under a laser scanning confocal microscope. EPCs were further documented by demonstrating the expression of KDR, VEGFR 2 and AC133 with flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis activity were assayed with MTT assay, modified Boyden chamber assay and in vitro vasculogenesis kit, respectively. EPCs adhesion assay was performed by replating MNCs on fibronectin coated dishes, and then the adherent cells were counted. Results Incubation of isolated human MNCs with high glucose concentration decreased the number of EPCs, and this effect was most prominant when glucose concentration was 45mmol/L, and incubated for 24 hours (approximately 1 fold decrease, P