Objective To analyze the hereditary background of common species from Rhodiola L. in Yunnan Province. Methods Fifty-nine samples of six populations in three natural medicinal species from Rhodiola L. were studied by RAPD method. Results The percentage of polymorphic bands (PPB) of three populations from R. crenulata were 37.97%, 46.15%, and 39.45% respectively; the Nei's gene diversities (h) were 0.110 3, 0.143 7, and 0.137 1, respectively; the Shannon's information indes (I) were 0.173 6, 0.222 1, and 0.206 7, respectively; at the special level, the PPB was 70.47%, I was (0.258 3); the genetic differentiation (G_(st)) was 0.263 0. The PPB of two populations from R. fastigiata were 43.67% and 44.42%, I were 0.215 3 and 0.217 4, respectively; at the special level, the PPB was 65.51%, G_(st) and I were 0.313 2 and 0.313 9, respectively; the PPB of R. yunnanensis was 36.97%, I was 0.188 1. Conclusion RAPD molecular marker could be used to molecular authentication and genetic diversity analysis of medicinal species from Rhodiola L.

Objective To investigate the application of heat-clearing and detoxifying Liangxue prescription decocting-free granules in puerperal infection and its clinical curative effect.Methods 97 cases patients with puerperal infection were chosen in the hospital from Dec.2012 to Dec.2014, according to the digital method, which were randomly divided into experimental group (n=49) and control group (n=48).The two groups were given metronidazole injection 250 mL/d, and 0.2 g mesylate levofloxacin added into 5% glucose injection 250 mL, intravenous drip, twice per day.On the basis of above therapy, experimental group were treated with heat-clearing and detoxifying Liangxue prescription decocting-free granules, orally, twice per day, one bag each time.Both of two groups were treated for 2 weeks as one course.Clinical symptom score, hemodynamic indexes, inflammatory factor, blood biochemical indexes and efficacy were observed and compared between two groups.Results Total effective rate of experimental group was 95.92%, which was significantly higher than 75.00% of control group ( U =3.58, P<0.01 ) .After treatment, the cell count of white blood cell ( WBC) and neutrophilic granulocyte ( GRAN ) , values of middle cell percentage (%MON ) , neutrophilic granulocyte ratio (%GRAN ) , and lymphocyte percentage (%LYM) in both of two groups significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the serum IL-6 and TNF-αlevels in two groups decreased compared with before treatment ( P<0.05 ) , and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the hemodynamic indexes including cell aggregation index, whole blood viscosity ( high, medium and low shear ) and plasma viscosity significantly improved compared with before treatment than control group ( P<0.05 ) , and the above indexes in experimental group significantly improved compared with control group (P<0.05).After treatment, the score of hypogastralgia, chills and fever, uterine body tender and a funicular tenderness of attachment area, lochia score and total score significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group significantly were lower than control group ( P<0.05 ) .Conclusion The heat-clearing and detoxifying Liangxue prescription decocting-free granules can significantly improve the inflammatory symptoms in treatment of puerperal infection, and its mechanism of action may improve microcirculation, reduce inflammatory reaction.The clinical efficacy of therapy is obviously better than western medicine treatment alone, which can be popularized in clinical application.

No abstract available.
Humans ; Hypogonadism* ; Male*

No abstract available.
Growth Hormone* ; Receptors, Somatotropin*

Objective To develop and validate a qual it y of life(QOL) scale for the Chinese patients with uterocervical cancer (QOL-UC C). Methods Based on the internationally accepted de finition of QOL and the clinical characteristics of uterocervical cancer as wel l as the existing QOL scales, we designed the QOL-UCC. Then 113 patients were r ecruited and the reliability, validity and feasibility of the scale were tested. Results It was shown that the QOL-UCC had good reli ability, validity and feasibility. Conclusion QOL-U CC is a reliable and valid instrument for evaluation of the QOL in Chinese pa tients with uterocervical cancer.

Objective To investigate the quality of life (QOL) of cervical cancer patients and its influential factors as well as to explore the method for improving the QOL in this population of patients. MethodsA QOL questionnaire for uterus cervical cancer (QOL-UCC) was designed and used in a survey of 81 UCC patients before they got any treatment and 124 UCC patients after they were treated for a period of time. SPSS 9.0 software was used to analyze data. ResultsThe patients got poor score with psychological status items(2.11? 0.34 ) before treatment, while those who had been treated for a period of time got poor score with social characteristics items(2.01? 0.33 ), especially with the work(1.87)and sexuality (1.00) items. Economic status of the patients, unsuccessful control of progress of the disease and recurrence were the main influential factors of the QOL in the UCC patients. ConclusionPsychosocial factors play very important roles in QOL of the UCC patients. As a result, psychosocial interventions should be stressed in the rehabilitation of this population of patients.

Object To study on the genetic diversity and genetic structure of Iphigenia indica Kunth. Methods Random amplified polymorphic DNA (RAPD) was applied to detect DNA fingerprints of three populations of I. indicafrom Yunnan Province. Results Twenty primers were screened, and a total of 120 DNA fragments were amplified ranging from 0.2-3 kb, among which 71 (59.17%) were polymorphic. The average number of DNA band produced by each primer was 3.55. The Shannon index was (0.199 4) in Ca population, 0.200 7 in Cb population, 0.254 8 in Cc population, respectively; the average value of populations was 0.218 3. The Shannon index was 0.288 6 in species. Nei's genetic identity was 0.930 9 between Ca and Cb, 0.932 7 between Ca and Cc, 0.946 6 between Cb and Cc. G_(st) within population was (0.204 4.) Conclusion For the establishment of protective tactic and measure, and of the standard of good agriculture practice (GAP) growth and heredity breeding, RAPD analysis provides I. indicawith theoretical foundation and basal data.

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Objective To observe the effect of stress caused by neuropathic pain on serum cortisol concentration and expres-sion of 11β-hydroxysteroid dehyarogenase 1(11βHSD1)and investigate the action mechanism of curcumin for alleviating neuropathic pain. Methods The 72 male SD rats were randomly divided into 4 groups with 18 rats in each group:sham operation group(Sham), chronic constrictive injury group(CCI),solvent contrast group(SC)and curcumin treated group(Cur100). Curcumin(100 mg/kg/d) was given after the operation in Cur100 group. Paw thermal withdrawal(PTWL)and paw mechanical withdrawal threshold(PMWT)of rats were measured on 2 pre-operative and 1,3,5,7,10,and 14 post-operative days. The animals were deeply anesthetized,the blood was taken from the heart,and the L4-L5 dorsal root ganglion(DRG)of the operated side was removed. The change of cortisol was measured by ELISA and the change of 11βHSD1 expression in spinal cord and DRG was determined by immunochemistry and Western blot. Results Compared with sham group,PTWL and PMWT decreased significantly after operation in CCI group(P<0.01),serum cortisol concentration was significantly increased(P<0.01)and the expression of 11βHSD1 was significantly increased in CCI group(P<0.01). Compared with CCI group,the PMWT and PTWL was increased in CCI group(P<0.05),the serum cortisol concentration was decreased(P<0.05)and the expression of 11βHSD1 was inhibited in Cur100 group(P<0.05). Conclusion Stress caused by neuropathic pain triggered the release of cortisol to the blood and increased the expression of 11βHSD1 at the same time. Cur-cumin could alleviate thermal hyperalgesia and mechanical hyperalgesia induced by CCI and could also inhibit the serum cortisol con-centration and the expression of 11βHSD1 in spinal cord and DRG,which was likely to be related to pathogenesy and maintenance of neuropathic pain.

This paper is to study the inhibitory effect of water soluble polymers--methyl cellulose (MC), hydroxypropyl methyl cellulose (HPMC), hydroxypropyl cellulose (HPC-M), poloxamer (F68) and polyvidon (PVP) on osthole (OST) crystallization and investigate the impact of polymer concentration and viscosity on crystallization behavior. Also, UV spectrophotometry method was used to determine the drug concentration at different time point to draw the OST concentration-time curve. Results show that HPMC has the most significant inhibition effect on OST crystallization, and drug concentration level is 1.61 times higher than that in control solution within 8 h followed by PVP (1.54) and MC (1.45) respectively. The kinetics of OST recrystallization can be described using first-order reaction, and the crystallization rate constants obtained by analyzing the regression equation indicate that HPMC-60SH-4000 and HPMC-60SH-10000 can greatly influence OST crystal formation. The dissolution rate of drugs precipitated from water-soluble polymer solutions is faster compared with controls in pH 1.2 HCl and pH 6.8 phosphate buffers, which demonstrated that water-soluble polymers can not only change the behavior of drug crystallization but markedly improve the dissolution rate of water insoluble drugs.