5.Expression of liver X receptor in the liver tissues of rat with multiple organ dysfunction syndrome induced by wound infection
Yu BAN ; Hong SHEN ; Tanshi LI
Chinese Journal of Emergency Medicine 2008;17(9):930-934
Objective To study the expression of liver X receptor (LXR) during the progress of multiple organ dysfunction syndrome (MODS) caused by wound infection, and its role in the lipid metabolism during MODS. Method The MODS models caused by wound infection were produced. One hundred Wistar rats were randomly divided into wound group (group T, n = 30) and MODS caused by wound infection group (group M, n = SO). The closed multi-fracture and extensive soft-tissue injury was produced in rats using jaw. After 12 hours, a thirty percent total body surface area Ⅲ (TBSA Ⅲ ) bum was induced in group M,and the rats were contaminated by pseudomonas aeruginosa. The levels of endotoxin ( LPS), alanine transaminase (ALT), free fatty acid ( FFA), TG, HDL, VLDL were determined before injuries and post injuries 24, 48, 96, 120 hours, and at each time point in each group, only 10 rats were determined. And at the same tune, real-time quantitative reverse transcription polymerase chain reaction technique was used to measure the gene expression of LXR. Chi-square test was used. Results The blood ALT and LPS levels in rats were increased slowly, the levels of FFA reached peak at 96 hours post injuries (P < 0.05), the level of TG increased first and then decreased to the lowest at 96 hours, HDL dropped slowly, and VLDL decreased first and then increased. The gene expression of LXR increased slightly at 24 hours and then gradually decreased in group T, and the gene expression of LXR decreased more obviously in group M, and there was significant difference compared with group T. Conclusions ALT increased slowly, and was positively correlated with IPS post injuries. This indicated that infection aggravated the liver injury. The gene expression of LXRα increased in the early stage and then decreased in the late stage, LPS and LXRα were negatively correlated, which may cause the change of LXRα. The decreased expression of LXRα during MODS may contribute to dysfunction of cholesterol transport and fatty acid synthesis.
6.Nursing rare of 12 newborns with thyroglossal cyst
Fengying YU ; Li LUO ; Hong JIANG
Chinese Journal of Nursing 2009;44(8):715-716
This paper summarize the perioperative nursing of 12 newborns with thyroglossal cyst. The preoperative nursing measures focused on prone position,gastric tube feeding,close monitoring of vital signs and tracheal intubation as needed. If the TeSO2 was normal,oxygen therapy was not recommended. After the operation,continuously monitoring vital signs,respiratory care and use of sedatives as needed were carried out. As a result,all of the 12 infants recovered and discharged.
7.The Measurement and Analysis of CT Linearity The Measurement and Analysis of CT Linearity The Measurement and Analysis of CT Linearity The Measurement and Analysis of CT Linearity The Measurement and Analysis of CT Linearity
Xiaoe YU ; Jun LI ; Deming HONG
Journal of Practical Radiology 2001;17(1):18-20
Objective:To measure CT linearity and evaluate if it would show linear relationship between coefficient and attenuation.Methods:The Catphan phantom was used to measure CT linearity in 18 computed tomography equipments,and the results were calculated.Results:(1)The CT linearity were qualified in 14 equipment but 4.Conclusion:(1)There are many factors which affect CT linearity.The artifact will be produced if the CT linearity is unqualified.(2)CT linearity must be calibrated periodically to assure the stability of the CT linearity and to make for the accurate diagnoses.