Objective To investigate the ureteral injury in gynecological laparoscopies and discuss its diagnosis,treatment and prevention.Methods Six cases of ureteral injury were reviewed.The clinical features including the type of diseases,uterine size,pelvic adhesion and the methods of diagnosis,treatment were studied.Results Ureteral injuries happened during laparoscopically assisted vaginal hysterectomy(LAVH). The gynecological disorders included pelvic adhesions,endometriosis and leiomyoma in cervical.Patients presented flank pain,fever,increased volumes discharge.Conclusion Ureteral injury is an uncommon and sever complication in gynecological laparoscopies.Fever,flank pain and abnormal vaginal discharge should be paid attention to be ureteral injury.

Objective To investigate the effect of nursing intervention on preventing leakage of urine in patients with indwelling catheter in neurology department.Methods A total of 76 patients with indwelling catheter in neurology department were randomly assigned into the observation group and the control group with 38 cases in each group.The control group received routine care,the observation group received systemic nursing intervention on the basis of routine care.The incidence of leakage of urine and patients satisfaction degree with nursing service was observed between two groups.Results The incidence of leakage of urine in the control group was 21.1％,higher than 2.6％ of the observation group.he score of satisfaction degree of the observation group was (4.66±0.62),higher than(3.26±0.42) of the control group.Conclusions Nursing intervention can effectively reduce the incidence of leakage of urine and improve the satisfaction degree of patients with indwelling catheter in neurology department,it is worthy of clinical application.

ObjectiveTo investigate the feasibility of apphcation of risk factors management in nursing of the fever outpatient.Methods145 patients from May to December 2009 admitted to fever clinic was defined as the control group, 157 patients from January to May 2010 admitted to fever clinic were set as the observation group. The control group was given routine nursing, while the observation group adopted risk factor management measures. The management indices of the two groups were compared.ResultsThe patients' recognition score of risk factors of fever clinic was (85.4 ± 2.7)in the observation group, higher than(73.2 ± 3.9)in the control group. Patients' satisfaction degree was 84.7％ in the observation group, higher than 67.6％ in the control group. Rate of risk event of the observation group was 1.3％, lower than 6.2％ in the control group, the incidence of nursing errors was 0.6％ in the observation group, lower than 4.1％ in the control group, rate of patients' complaints of nurses was 1.3％ in the observation group, lower than 5.5％ in the control group.ConclusionsThe implementation of risk factors management in fever clinics can reduce medical accidents, improve nursing quality, so this clinical management mode deserves promotion and application.

Objective To investigate the epidemiology and clinical features of epidemic cerebrospinal meningitis of children in Shanghai area in the last 2.5 years. Methods A retrospective study was conducted in 27 hospitalized patients with epidemic cerebrospinal meningitis by analyzing the clinical manifestation,epidemiology and laboratory test fingdings from Jan.2006 to Jun.2008.Statistical analysis of the clinical manifestations of cases in serogroup A,B and C was performed according to Neisseria meningitidis infection. Results There were sporadic cases of children with epidemic cerebrospinal meningitis in Shanghai area in the last 2.5 years.The seasonal distribution illustrated the peak occurrence from Feb.to Apr.Among all these cases,92.6% were floating children.Common types(74.1%) were the predominant clinical manifestations and the others were fulminate types(25.9%).Four cases in each of serogroup A and B,and 8 cases of serogroup C were identified by serotyping on 14 Neisseria meningitidis strains and genotyping on the other 2 cases′ cerebrospinal fluid specimens.All the patients in serogroup A with meningococcal diseases were not vaccinated before and 25.0% of them belonged to fulminate types.Half of the cases had petechia on the skin and the prevalence of skin necrosis was 25.0% and 50% had complications of nervous system.No fulminate type was present in cases of serogroup B meningococcal disease and only 1 case had slight petechia on the skin.However,intensive convulsions occurred in 75.0% of the patients,and all of them had complication of nervous system,with a few had sequela.Among the patients in serogroup C meningococcal disease,62.5% patients were vaccinated with serogroup A Neisseria meningitidis.In serogroup C,Neisseria meningitidis infection was found in each age group,with petechia on skin,skin necrosis,immunity correlation fever and complications of nervous system in 87.5%,50.0%,75.0% and 12.5% of the patients,respectively. Conclusions 1.Epidemic cerebrospinal meningitis occurred sporadically in children in Shanghai area,the majority of patients were floating populations.2.Clinical manifestation was typical in patients of serogroup A meningococcal disease.Subdural effusion or ventricalitis was more common in patients of serogroup B meningococcal disease.In serogroup C,Neisseria meningitidis was the most prevalent serotype leading to epidemic cerebrospinal meningitis.More cases were found in patients infected with serogroup C,Neisseria meningitidis with severe shock and immunity correlation fever compared with the other groups.Prophylactic vaccination of serogroup C polyvalent meningococcal organism should be recommended.

Objective To study the effect of H_2O_2 on the cultured melanocytes and the relationship between oxidative stress and vitiligo.Method Melanocytes were cultured with H_2O_2,the proliferation and melanogenesis of melanocyte were analysed by MTT and NaOH assay.Result H_2O_2 can inhibite the proliferation and melanogenesis of melanocytes,and the inhibition was concentration and-time-dependent.Conclusion Oxidative stress can inhibite the proliferation and melanogenesis of melanocytes,but oxidative damage can not induce vitiligo.

To analyze the specific mechanism of Jinxueyuan granules, the relationship between the Jinxueyuan granules increased the saliva secretion of xerostomia model SD rats and excitement of receptors were studied in this experiment. In the study, three groups of xerostomia model rats were successfully established by using M-receptor blockers-4-diphenyl-acetoxy-N-methyl-piperidine (4-DAMP) and atropine, or adrenergic receptor blocker phentolamine; after the modeling, the medicine Jinxueyuan granules were gavaged. According to the clinical dose of Jinxueyuan granules and SD rats body surface area, the rats in atropine group were divided three dose groups respectively, namely low, medium and high dose of Jinxueyuan granules groups. The 4-DAMP group and phentolamine group were gavaged medium dose of Jinxueyuan granules. And the amount of salivary secretion for 150 minutes in all groups continuously were measured, and the effect of Jinxueyuan granules increased salivation and the relationship between characteristics and the receptors were observed; and submandibular gland tissue of the rats was isolated, then the effect of Jinxueyuan Granules for expression of the water channel protein aquaporin-5 (AQP5) in submandibular gland cells was analyzed by the Western blot technology. It was found that the saliva secretion of Jinxueyuan Granules groups was increased significantly, and compared with the saline control group, phentolamine group, 4-DAMP group and atropine group, difference was significant, P < 0.05. There was no significant difference between the low-dose of Jinxueyuan granules group and the saline group, but the medium dose of Jinxueyuan granules group had a significant difference, compared with the saline group (P < 0.05). In the time distribution of increasing saliva secretion, there was a significant difference between the saline and Jinxueyuan granules group in the saliva secretion (P < 0.05). After administration of Jinxueyuan granules, the expression of AQP5 protein in the submandibular gland cells expressing of treatment groups was increased, and compared with the blocker groups, there was a significant difference, P < 0.05. Except the atropine group, there was no significant difference in Jinxueyuan granules relieving the inhibition induced by blocks in phentolamine group and 4-DAMP group, compared with the saline group. Compared the AQP5 expression in three blockers groups, there was no significant difference in the efficacy of Jinxueyuan granules between phentolamine group and 4-DAMP group; but there was a significant difference between the atropine group and other groups (P < 0.05). Therefore, it was considered that the mechanism of Jinxueyuan granules increasing saliva secretion (effectiveness of nourishing Yin and generating body fluid ) possibly through the pathway mediated by muscarinic M receptor, especially M3 receptor, or adrenergic receptor, and increased expression of salivary gland AQP5 membrane, and then stimulate saliva production.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Rats ; Saliva ; secretion ; Salivary Glands ; drug effects ; secretion ; Xerostomia ; drug therapy ; metabolism

Dental Porcelain ; classification ; Humans

Objective To explore the functional expression and temporality of MDR1 gene in bone marrow of rabbits after autologous bone marrow transplantation with MDR1 transferred bone marrow mononuclear cells. Methods The supernatant of the amphotropic virus producer cell line PA317-HaMDR1/A was collected and concentrated to cocultivate with the bone marrow mononuclear cells of the rabbits. After large dose of chemotherapy with cyclophosphamide,the transferred cells were autotransplanted into the bone marrow. The integration,transfection rate and physiological function of MDR1 gene were tested by PCR,SP immunohistochemical method and daunorubicin (DNR) extrusion test respectively. Results After autologous bone marrow transplantation had been executed for 1-4 months,the integration of MDR1 gene in genome of bone marrow mononuclear cells was detected by PCR,and the expression rates of P-gp in cells tested by SP immunohistochemical method were 9.5%,8.5%,6.0% and 3.5% respectively. The physiological function of MDR1 gene in bone marrow cells was proved by DNR extrusion test. Conclusion After the autotransplantation with bone marrow mononuclear cells transferred by MDR1 gene,the MDR1 gene can implant into the bone marrow of rabbits and has expressed functionally for 4 months,which has provided a basis for further research on chemoprotection experiment of the MDR1 gene transferred into the bone marrow cells.

Objective To investigate the effects of short-segment pedicle screws system on type A thoracic and lumbar spine fracture with or without pedicle screws on the fractured level. Methods 23 patients with type A thoracic and lumbar spine fracture were included, in which 11 cases received traditional short-segment pedicle screws system without pedicle screws on the fractured level (group A), and other 12 cases with pedicle screws on the fractured level (group B). The anterior vertebral height, posterior vertebral height and local kyphosis an-gle were measured before and after operation. Results All pedicle screws were successfully implanted with good location and stability. The vertebral height and kyphosis angle significantly improved after operation in both groups (P<0.05), and the improvement of kyphosis angle was more in group B than in group A (P<0.05). Conclusion Short-segment pedicle screws system with pedicle screws on the fractured level may fine correct the kyphosis angle in short term.

Objective To explore the impact of different anesthesia methods and anesthesia-related factors in initial bladder cancer patients with partial cystectomy, and to provide a reference for clinical anesthesia choice and the questions that should be concerned. Methods Ambispective cohort study was performed in consecutive case of illness with initial occurrence bladder cancer patients treatedwith partial cystectomy from 2009 to 2012. A historical cohort was from January 5, 2009 to January 4, 2011. A prospective cohort was from January 5, 2011 to December 31, 2011. Follow-up was terminated in December 31, 2012. All cases were followed up to one full year after surgery. The 402 cases who were diagnosed of initial occurrence bladder cancer and partial cystectomy patients, according to different anesthesia method, were divided into general anesthesia group and intraspinal anesthesia group. Postoperative recurrence was determined within 1 year of follow-up. Multivariate analysis was carried out based on univariate analysis to analyze the causal connection factors for one year recurrence in initial occurrence bladder cancer patients with partial cystectomy. Variables included gender, age, blood type, smoking history, drinking history , number of tumors, tumor size, pathologic TNM stage, anesthesia method, duration of anesthesia, postoperative visual analog scale (VAS) score at 12 h, tumor-associated factor Ki67, P53, CK20, VEGF (whether two or more positive). Tumor recurrence was considered the dependent variable (outcome). Univariate analysis used χ2 or rank sum test, and multivariate analysis used Logistic regression model fitting analysis and the maximum likelihood method. SPSS 16.0 software package was used for data analysis and processing. Results Four hundred and two patients were followed up and got a result of 396 cases (98.51％). Two hundred and eleven cases showed recurrence within one year (53.28％). Univariate analysis showed that age, smoking history, drinking history, duration of anesthesia, pathologic stage, postoperative VAS scores at 12 h less than 3 scores, blood type, tumor-associated factor Ki67, P53, CK20, VEGF (two or more positive) were associated with one year recurrence in initial occurrence bladder cancer patients (P < 0.01) with partial cystectomy, while the difference of two anesthesia methods was not shown (P>0.05). Multivariate Logistic regression model analysis showed that the following factors were associated with postoperative recurrence within a year in initial occurrence bladder cancer patients with partial cystectomy: age (RR = 0.371, 95％ CI 0.183-0.752), drinking history (RR=0.348, 95％CI 0.161-0.752), postoperative VAS scores at 12 h less than 3 scores (RR=0.023, 95％CI 0.011-0.050), duration of anesthesia (RR=3.042, 95％CI 1.495-6.192), and tumor-associated factor Ki67, P53, CK20, VEGF of two or more positive(RR=8.676, 95％CI 4.018-18.733). Among these, duration of anesthesia and tumor-associated factors Ki67, P53, CK20, VEGF of two or more positive were risk factors, and drinking history, age, and postoperative VAS scores at 12 h less than 3 scores were protection factors. Conclusions The impact of general anesthesia and intraspinal anesthesia on one year recurrence in initial occurrence bladder cancer patients with partial cystectomy is not statistically significant. Duration of anesthesia depends on the part of the surgical time which is associated with the postoperative recurrence within a year. Postoperative analgesic perfect (VAS score at 12 h less than 3 scores) is the protective factor. Age and drinking history are also the protective factors for one year recurrence in initial occurrence bladder cancer patients of partial cystectomy.