Objective To evaluate the expression of annexin V in maternal blood and placenta,and to explore the relationship between annexin V and preeclampsia(PE). Methods 120 women with PE who delivered babies in the Second Hospital of Hebei Medical University from December 2007 to December 2009 were chosen as study groups. They were classified into four groups: early-onset mild group (n =30),early-onset severe group (n=30), late-onset mild group ( n = 30) and late-onset severe group (n=30). 30women without perinatal complications who accepted elective term cesarean section were chosen as control group. Western blot and immunohistochemistry were used to detect the expression and localization of annexin V in placenta and maternal blood. Flow cytometry was employed to detect the apoptosis of cytotrophoblast.Annexin V mRNA level was determined by reverse transcription (RT)PCR. Prothrombin time (PT),activated partial thromboplastin time (APTT), fibrinogen (FiB), international normalized ratio (INR) were detected in each group. Results (1) The expression of annexin V in placenta and maternal blood were:0.54±0.12 and 0.62±0.17 in early-onset mild group; 0.47±0.15 and 0.56±0.24 in early-onset severe group; 0.74±0.23 and 1.08±0.32 in late-onset mild group; 0.68±0.28 and 0.72±0.21 in late-onset severe group;1.73±0.35 and 1.55±0.27 in control group. They were significantly lower in PE groups than in control group (P＜0.05 ). However, there was no significant difference among PE groups (P＞0. 05). (2) The early apoptosis, late apoptosis percentage of trophoblast cells were: 3. 21%, 0. 86%, in early-onset mild group; 5.32%, 0. 72%, in early-onset severe group; 2. 43%, 0. 63%, in late-onset mild group;4. 28%, 0. 48% in late-onset severe group; 1.05%, 0. 59%, in control group. Early apoptosis percentage in each group of PE was higher than that in control group ( P ＜ 0. 05 ). However, there was no significant difference among PE groups (P＞0.05). (3) The annexin V mRNA levels in placenta were:25.0±3.0 in early-onset mild group; 24. 8 ± 3.0 in early-onset severe group; 25.4 ± 3. 9 in late-onset mildgroup; 25.1±2.7 in late-onset severe group, respectively. All were significantly higher than that in control group (30. 6±3.0, P＜ 0. 05), and no significant difference was found among PE groups (P＞0.05).(4) PT, APTT, FiB, INR levels were: (11.3±2.4), (25.6±2.9) s, (4.6±0.9) g/L and 0.9 ±0.2in early-onset mild group; (12.1±1.9), (27. 2 ±2. 1 ) s, (5.0 ± 1. 0) g/L and 0. 9 ±0. 2 in early-onset severe group; (11.7±2.3), (26.5±2.3)s,(5.0±0.7)g/L and 0.8±0.3 in late-onset mild group;(11.4±2.6), (27.3±3.0) s, (4.3 ±0.8) g/L and 0.8 ±0.3 in late-onset severe group; (12.4±2.7), ( 28.0±1.9) s, (5.1±1.2) g/L and 0.9 ± 0.2 in control group. There was no significant difference among PE groups and control group ( P ＞ 0.05 ). Conclusion The expression changes of annexin V in placenta and maternal blood were observed in patients with PE. This indicated that annexin V played an important role in the pathogenesis and progression of PE by affecting coagulation.

OBJECTIVE: To explore the clinical application value of using needle electrode in transurethral plasmakinetic resection of bladder tumor around ureteral orifice. METHODS: Retrospective analysis was performed on the clinical data of 16 cases who had bladder tumors around ureteral orifice and underwent transurethral resection using plasmakinetic needle electrode in Department of Urology, Peking University International Hospital from June 2015 to December 2019. There were nine cases with the tumor of one to two centimeters from the ureteral orifice. The rest of the seven cases had tumor that was within one centimeter from the ureteral orifice, including two cases whose ureteral orifice was invaded by the tumor. All the patients studied were diagnosed before surgery and contraindications were excluded. The plasmakinetic needle electrode was used to treat the tumor with en bloc resection, and all the excised tissue was sent for pathological examination. Intravesical chemotherapy and postoperative follow-ups were performed. Statistical analysis was performed on the operation time, the incidence of obturator nerve reflex, the peri-operative bleeding, the parameters of indwelling ureteral catheter or double-J stent, the incidence of postoperative hydronephrosis, the clinical stage of tumor, and the recurrence rate. RESULTS: The operation was successfully completed for all the sixteen cases. The operation time was 16 to 57 minutes, with an average of (32.6±11.8) minutes. No obvious obturator nerve reflex and perioperative bleeding occurred in all the patients. Ureteral catheters were indwelled prior to the operation of tumor resection in seven cases. Four of the seven cases had the ureteral catheters remained while the rest three were replaced by double-J stent after surgery. Postoperative pathological analysis showed that all the tumors were urothelial carcinoma, including 9 cases of low grade and 7 cases of high grade. Pathological staging: 10 cases were in Ta stage, 5 cases in T1 stage, and 1 case in T2a stage. All tumor bases and lateral margins were negative. All the patients received 3-56 months, with an average of (26.0±18.1) months of follow-up. There was no case of upper urinary tract hydronephrosis or tumor recurrence. CONCLUSION The transurethral plasmakinetic resection of bladder tumor using needle electrode can realize en bloc tumor resection without obturator nerve reflex and reduce the risk of ureteral orifice injury. It is a safe and effective surgical method for treating bladder tumors around the ureteral orifice.
Carcinoma, Transitional Cell ; Electrodes ; Humans ; Neoplasm Recurrence, Local ; Retrospective Studies ; Urinary Bladder Neoplasms/surgery*

Objective: To separate and determine two constituents of Danshensu and protocatechuic aldehyde in compound Danshen Dropping Pill. Methods: The chromatographic conditions were as follow: Nucleosil C18 column, a mixture of methanol-water-acetic acid glacial (19∶80∶1) as the mobile phase with a flow rate of 1 mL per minute and the detection wavelength at 279 nm. P-hydroxybenoic acid was used as internal standard. Results: The calibration curves were linear in the range of 10～80 mg/L (r=0.9995) for Danshensu and 2～16 mg/L (r=0.9996) for protocatechuic aldehyde . The average recovery of Danshensu and protocatechuic aldehyde were 99.4 %and 99.5 %respectively. Conclusion: This method is accurate, simple and convenient, rapid and reproducible and can be used for the quality control of compound Danshen Pill.

Objective To evaluate the clinical effect of Liyanjiedu decoction for the prophylaxis and treatment of acute radiotherapy-induced oral mucositis of nasopharyngeal carcinoma(NPC)and the influence to the effect of the radiotherapy to NPC.Methods A total of 96 patients with NPC were randomly divided into treatment group and control group,48 cases for each group.The two groups were given radical radiotherapy.The treatment group was given Liyanjiedu decoction for buccal.The control group were given western medicine for mouthwash.The course of two groups was all from the beginning of radiotherapy to the end of it.Results The occurring time of radiotherapy-induced mucositis in treatment group and control group were(21?4.2)d and(16?3.6)d,and radiotherapeutic dose were(40.252?11.758)Gy and(29.625? 11.632)Gy,with significant difference between the tow groups(P

Objective To develop a new,three dimensional dynamic system for pulse detection.Methods According to bionics,a multi-information sensing mode with a soft surface is designed on the basis of traditional Chinese medical diagnosis in pulse.This mode imitates the touching sense of human finger and with the application of modern computer information processing technique,the pulse information is extracted in the way that is as close as possible to the traditional Chinese medicine pulse diagnosis.Results With the application of new sensor,pulse signal is detected and the effectiveness of the inovative three-dimension data transimition mode is validated since the pulse signal is three-dimensionally re-displayed on the computer screen.Conclusion The method introduced in this paper is proved feasible for detecting pulse information.Expected results are obtained and the method provides a new way and new feature reference for research and teaching in objectifying the diagnosis of traditional Chinese medical sciences.

Objective:To evaluate the clinical efficacy of treatment of sudden deafness using hyperbaric oxygenation, and to provide guidance for its treatment. Methods:The studies about sudden deafness treated with hyperbaric oxygenation combined with drug were retrieved in Cochrane Library, PubMed , MEDLINE, Wanfang Database and CNKI up to July 2016.RavMan 5.3 provided by Cochrane was used for Meta-analysis.Results:Thirty-nine studies which were randomized controlled trials (RCTs)were included, a total of 4 599 cases,of which 2 418 cases received hyperbaric oxygenation therapy combined with drug treatment as treatment group and 2 181 cases received drug treatment alone as control group.A total of 36 studies about the comparison of clinical efficacy between treatment group and control group were included in the Meta-analysis, the result showed that treatment group had a significant clinical efficacy than control group(RR:1.26,95%CI:1.22-1.30,P<0.0001).A total of 6 studies about the improvement of the pure tone average(PTA)in the two groups were included in the Meta-analysis, the PTA gain was significant better in hyperbaric oxygenation group(WMD:11.8,95%CI:4.77-18.83,P<0.000 1).A total of 11 studies about the stage of using hyperbaric oxygenation therapy were included in the Meta-analysis, the result suggested that the early use of hyperbaric oxygenation therapy had better effect than the late use of hyperbaric oxygenation (RR:1.42,95%CI:1.31-1.53,P<0.000 01).Conclusion:The clinical efficacy of hyperbaric oxygenation combined with drug therapy is better than drug therapy alone, the PTA gain is significantly improved and the introduction of hyperbaric oxygenation therapy at the earlier stage shows better results.

Bacteremia ; diagnosis ; Humans ; Infant, Newborn ; Male ; Pseudomonas Infections ; diagnosis

AIM:To analyze the cause of posterior capsular rupture in small incision non - phacoemulsification cataract surgery and investigate the treatment methods.
METHODS: The clinical data of 108 cases ( 121 eyes ) undergone small incision non - phacoemulsification cataract surgery combined with intraocular lenses ( IOL) implantation were retrospectively analyzed. The causes of posterior capsular rupture and treatment of 6 patients (6 eyes) after surgery were analyzed and summarized.
RESULTS: Six cases ( 6 eyes, 4. 96%) occurred posterior capsular rupture during the operation, of which 2 cases ( 2 eyes ) underwent posterior chamber IOL implantation intraoperative, 2 cases (2 eyes) receivedIIstage IOL implantation in the ciliary sulcus at 1wk postoperative, 2 cases ( 2 eyes ) were given anterior chamber IOL implantation. One case ( 1 eye ) whose vision acuity <0. 1 was macular degeneration; One case ( 1 eye ) whose vision acuity = 0. 3 was diabetic retinopathy;0. 4~0. 6 in 2 cases (2 eyes), 0. 6~0. 8 in 2 cases (2 eyes) after 1 ~3mo postoperatively. No severe syndrome was found after surgery.
CONCLUSION:Posterior capsular rupture which occurs in different stages of the operation is the commonest intraoperative complication in small lincision non -phacoemulsification cataract surgery. With improving operative techniques, and continuously accumulating experience, incidence of posterior capsular rupture can be effectively reduced. Even posterior capsular rupture occurs intraoperative, patients can still get a good outcome after timely and proper treatment.

Synovial sarcoma is a rare tumour found in soft tissue; it is a mesenchymal spindle cell tumour that is not related to the synovial membrane. This tumour has a low incidence, and the most frequent place of occurrence is the lower extremities in young adults. Synovial sarcoma of the head and neck accounts for 3%-5% of sarcomas in this anatomical region. The tumor in the nasal cavity is less than 1%. The treatment of choice for synovial sarcoma of the head and neck is complete surgical excision of the tumour mass followed by adjuvant radiotherapy.
Humans ; Nasal Cavity ; pathology ; Nose Neoplasms ; diagnosis ; radiotherapy ; surgery ; Paranasal Sinuses ; pathology ; Radiotherapy, Adjuvant ; Sarcoma, Synovial ; diagnosis ; radiotherapy ; surgery