Objective: To construct the Behavior Inhibition Scale. Methods: The compilation of items was in accordance with the content validity of seven kinds behavior inhibition. The Self Consistency and Congruence Scale and SCL-90 were also applied as validity index. Results: Factor analysis on 660 subjects rating on original 86 items of Behavior Inhibition Scale yielded seven factors composed of 42 items, measuring the inhibition of help-seeking, self-confidence, independence, negativeness, alienation, dominate, and refuse, respectively. Behavior inhibition correlated significantly with SCL-90 and self congruence, and significant sex and education differences were also detected. Conclusion: The Behavior Inhibition Scale has good reliabilities and well validated.

Objective: To investigate the characteristics of Behavior Styles personality dimension among subjects of dif- ferent age, genders, marriage status, and occupations. Methods: After subjects fulfilled Chinese Personality Scale (QZPS), score differences of Behavior Styles and its sub- factors were compared among age, genders, marriage status, and occupa- tions. Results: Significant differences were detected among different age, genders, and occupations. Males have lower scores than females; the elder the subjects, the higher the scores; executives and technicians have the same scores, and both have higher scores than workers and peasants. Some interactions were also significant. Conclusion: There were sig- nificant differences among subjects of different gender, age, and occupation.

Objective To investigate the main predisposing factors of hematoma enlargement in spontaneous intracerebral hemorrhage,reduce the early mortality,and improve clinical outcomes.Methods By a retrospective study of 268 patients,all patients underwent their first computed tomography (CT)scan within 24 hours of arrival and a second CT scan within 72 hours of admission.These predisposing factors were analyzed by means of univariate and multivariate logistic regression analyses.Results The patients occurred early hematoma enlargement were accounted for 27.99％ (75/268).Univariate analyses indicated that hematoma shape,midline shift,breaking into the ventricle,thrombin clotting time (TT),white blood cells,neutrophil percentage,serum creatinine,fasting plasma glucose (FPG),and Glasgow coma scale (GCS) were significantly different between the patients with or without hematoma enlargement (P ＜0.05).Furthermore,multivariate logistic regression analyses revealed that the following four factors were independently associated with hematoma enlargement:the midline shift [OR (95％ CI) =3.356 (1.251-8.999),P =0.000],the GCS score [OR (95 ％ CI) =0.745 (0.671-0.827),P =0.023],the FPG [OR (95％ CI) =3.022 (1.232-7.408),P =0.016],and the breaking into the ventricles [OR (95％ CI) =2.851(1.158-7.019),P =0.005].Conclusions The midline shift of brain CT scan,the GCS score,the FPG,and the breaking into the ventricles maybe the independent predisposing factors of hematoma enlargement.Therefore,timely review of CT scan and dynamic monitoring of peripheral blood biochemical indicators should be very important for further therapy.

Concurrent thyroid carcinoma and parathyroid adenoma is rare, they can and do coexist. We present here a 63-year old man who had bilateral papillary thyroid carcinoma and a parathyroid adenoma in the right thyroid lobe. Thyroid cancer was confirmed surgically. After the operation, the patient was found hypercalcemie and hypophosphatemia along with an elevated parathyroid hormone (PTH), indicating primary hyperparathyroidism. Also, the parathyroid 99mTc-MIBI scan demonstrated parathyroid adenoma in the right lower pole of the thyroid. The abnormal parathyroid tissue was carried out, and then serum calcium and PTH levels decreased to normal ranges.
Calcium ; blood ; Carcinoma ; pathology ; surgery ; Carcinoma, Papillary ; Humans ; Hyperparathyroidism, Primary ; Male ; Middle Aged ; Parathyroid Glands ; pathology ; Parathyroid Hormone ; blood ; Parathyroid Neoplasms ; pathology ; surgery ; Thyroid Cancer, Papillary ; Thyroid Neoplasms ; pathology ; surgery

Objective Through investigating the situation of health care resources in Shanghai ,from the point of population and geographical area distribution, the article analyze the fair allocation of medical resources in Shanghai to provide the basis of decision-making in rational allocation of medical resources. Methods Use Lorenz curve and the Gini coefficient indicators, as well as Chain Proportion Method. Results The allocation of medical resources in population of Shanghai is fairly,and the trend of fairness will be enhanced. In the geographical allocation in space is more unfairness in Shanghai,and the fairness will be reduced. Conclusion The allocation of medical resources Municipal Population has few gap between urban and rural areas in the Shanghai which reached a fair comparison, but in the geographic distribution of has a larger gap between urban and rural areas. Most of the medical resources concentrated in large hospitals in the urban centers. The distribution of Medical resources should be rational and the health service system should be improved to improve medical and health service accessibility.

Aim To investigate subcellular distribution and accumulation of ADR in the sensitive and multidrug-resistant HL-60 cells and its relation to multidrug resistance.Methods The subcellular distribution and accumulation of ADR were studied by confocal scanning laser microscope and flow cytometry.The effects of verapamil,BSO,brefeldin A and chloroquine on ADR distribution and accumulation in HL-60/ADR cells were also examined.Rhodamine123,NBD-ceramide and neutral red were used as fluorescent probes to stain the mitochondria,Golgi apparatus and lysosomes respectively were used to identify the subcellular compartments where ADR was sequestered.Results In drug-sensitive cell line HL-60,ADR fluorescence distributed evenly in the nucleus and cytoplasm,while in multidrug-resistant cell line HL-60/ADR,ADR fluorescence distributed in a punctated pattern in the cytoplasm and was reduced in the nucleus.The mode of ADR distribution in HL-60/ADR cells is highly similar to that of NBD-ceramide.BSO and brefeldin A,instead of verapamil and chloroquine could reverse the abnormal distribution and accumulation of ADR in HL-60/ADR cells.Conclusions The change of ADR distribution and reduction of ADR accumulation in multidrug-resistant cell line was involved in the mechanism of multidrug resistance.

Objective:To investigate the effects of survivin antisense oligonucleotide (ASODN) on expression of survivin and ACC-M cell apoptosis. Methods: A phosphorothioate antisense oligonucleotide (ASODN) of specific target survivin was designed and synthesized and then transferred to ACC-M cell line by lipofectin. At the same time blank control group, sense oligonucleotide (SODN) group were set up for comparison. MTT assay was used to detect cytotoxicity. Apoptosis was observed by flow cytometry. Survivin expression was determined by RT-PCR and Western-Blotting. Results: Compared with control group and SOND group, in ASODN groups, the expression of survivin mRNA and protein were obviously weak, apoptosis rate apparently increased, cells growth was inhibited. There was no obviously difference in SODN and control groups.Conclusion:ASODN can down-regulate the expression of survivin gene in ACC-M cell line specifically. It plays an important role in inducing tumor apoptosis and suppressing cell proliferation.

Objective To investigate the effect of autophagy on histone-mediated apoptosis of human proximal tubular endothelial cells(HK-2).Methods To investigate the effect of histones on the autophagy and apoptosis,HK-2 cells were treated with increasing concentrations of histones.The rate of apoptosis and the expressions of autophagy-related protien LC3Ⅱ and Beclin1 in HK-2 cells were detected by using flow cytometry and immunoblotting assay,respectively.To further confirm the effect of autophagy on apoptosis of HK-2 cells,cells were incubated with histones after one hour pretreatment with 10 mmol/L 3-MA,a pharmacological inhibitor.The rate of apoptosis and the activity of caspase-3 of HK-2 cells were detected separately by using flow cytometry and immunoblotting assay.Results Histones significantly enhanced apoptosis of HK-2 cells in a dose-dependent manner,with the increased expressions of LC3Ⅱ and Beclin1.Blockage of autophagy by 3-MA significantly increased the apoptosis of HK-2 cells and the activity of caspase3.Conclusion Autophagy in proximal tubules protects against apoptosis induced by histones,with potential value in acute kidney injury (AKI).

AIM: To investigate the effect of 17?-estradiol(E2) on myocardial hypertrophy induced by endothelin-1(ET-1) and the related mechanism.METHODS: Myocardial cells from neonate rats were cultured in vitro and myocardial hypertrophy model was established with ET-1.The effects of 17?-estradiol on myocardial hypertrophy were observed.The role of ERK1/2 in the effects of 17?-estradiol was also detected.RESULTS: Compared with control group,ET-1 increased cell protein content,cell surface area and -Leucine(-Leu) incorporation.Pretreatment with E2 for 24 h could inhibit the increase in cell protein content,cell surface area and -Leu incorporation induced by ET-1.ET-1 significantly stimulated ERK1/2 activity,which was prevented by pretreatment with E2.Tamoxifen,estradiol receptor antagonist,partially inhibited the effect of E2.The ability of ET-1 to stimulate -Leu incorporation was significantly blocked by PD98059,which could enhance the inhibitory effect of E2 on the increase of -Leu incorporation in cardiomyocytes induced by ET-1.CONCLUSION: E2 can inhibit cardiomyocyte hypertrophy induced by ET-1.This effect is mediated by estrogen receptor.ERK1/2 signal pathway is closely correlated with the inhibitory effect of E2 on cardiomyocyte hypertrophy induced by ET-1.

OBJECTIVE To study the correlation between the expression level of Cyclin G2 and the laryngeal squamous cell carcinoma.METHODS The clinical data and paraffin-embedded tumor samples of 81 patients with LSCC treated at Shenyang 463 Hospital between Jan. 2000 to Dec.2000 were retrieved.Cyclin G2 expression was analyzed immunohistochemically in tumor tissues and adjacent normal laryngeal mucosa.The relationship between Cyclin G2 expression and clinical data including age,gender,tumor stage,pathological type,and prognosis were analyzed using software SPSS12.0.RESULTS The positive rate of Cyclin G2 in laryngeal squamous cell carcinomas was 66.7% and it was correlated with tumor differentiation,lymph node metastasis(both P value less than 0.05).CONCLUSION The expression of CyclinG2 correlates with the malignant degree and development tendency of LSCC negatively.Cyclin G2 is an important biological factor affecting the prognosis.