To elucidate the expression of WT1 in all types of leukemias and its implications for monitoring minimal residual disease in patients with acute leukemia, the peripheral blood from 55 leukemia patients and 10 normal voluteer was detected by using FQ-RT-PCR. Follow-up monitoring of WT1 expression of peripheral blood was performed for 20 patients with acute leukemia. The results showed that the expression of WT1 gene in all types of leukemias was significantly higher than that in normal control (P < 0.001). For ANLL and ALL patients, the survival time in the group of WT1 6.8 x 10(-3), (P = 0.027). Follow-up detection of the expression of WT1 in peripheral blood samples from 20 acute leukemia patients, 7 cases relapsed after complete remission has been done. In 5 of 7 relapsed patients, the expression of WT1 had obviously increased about 2 - 3 months before clinical relapse became apparent. It is concluded that the established FQ-RT-PCR method is accurate and specific. The expression of WT1 gene is relatively high in all types of leukemias compared with normal peripheral blood cells, the higher WT1 expression may associate with poor prognosis in acute leukemia, and the dynamics of WT1 level correlate with the disease status. The quantitative assessment of WT1 expression in peripheral blood samples by FQ-RT-PCR may be a useful tool for monitoring minimal residual disease.
Acute Disease ; Adolescent ; Adult ; Aged ; Biomarkers, Tumor ; genetics ; Child ; Female ; Gene Expression Regulation, Leukemic ; Humans ; Leukemia ; blood ; genetics ; pathology ; Male ; Middle Aged ; Neoplasm, Residual ; blood ; diagnosis ; genetics ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; methods ; Sensitivity and Specificity ; WT1 Proteins ; genetics

OBJECTIVETo find a satisfied and applicable repairing method for big auricle defect.

METHODSan auricular posterior flap with has two subcutaneous pedicles on mastoidea, was applied for repairing of big auricle defect. A framework of rib cartilage was embedding into the flap to shape auricle.

RESULTSatisfied result was abtained in all 12 cs-es.

CONCLUSIONUsing auricular posterior flap with two subcutaneous pedicles to repair big auricular defect is a satisfied and applicable method.

Adolescent ; Adult ; Ear Auricle ; injuries ; surgery ; Humans ; Male ; Mastoid ; surgery ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; Surgical Flaps ; Young Adult

OBJECTIVETo analyze ultrasonographic features for the complications of breast augmentation with polyamide hydrogel injection.

METHODSClinical study was made for 101 patients who had received the polyamide hydrogel injection for augmentation mammoplasty by using the ultrasonographic examination.

RESULTSThe 195 treated breasts in 98 patients suffered from injectable layer disorder, 1 breast for hematoma, 7 breasts in 5 patients for abscess (infection), 2 breasts in 2 patients for cancer and 3 breasts in 3 patients for fibroadenomas.

CONCLUSIONSUltrasonography may be of great diagnostic value for the complications of breast augmentation with polyamide hydrogel injection.

Acrylic Resins ; adverse effects ; Adult ; Breast Implants ; adverse effects ; Female ; Humans ; Mammaplasty ; adverse effects ; methods ; Middle Aged ; Postoperative Complications ; diagnostic imaging ; Ultrasonography, Mammary ; Young Adult

OBJECTIVETo evaluate the application of ultrasonography in the detection of nasal interdomal fat pad (IFP).

METHODSThirty-eight patients who underwent open rhinoplasty and other surgeries in our hospital were enrolled in this study. All patients were studied by ultrasonography to evaluate the shape, structure, size, and location of IFP. The results of ultrasonography were compared with intraoperative findings.

RESULTSThe boundaries between IFP and the subcutaneous fat were clear under ultrasonography in 32 patients (84.2%), but were not clear in 6 patients (15.8%) who had undergone injection rhinoplasty (n=2) or augmentation rhinoplasty (n=4). As shown by ultrasonography,the average length,width,and height of IFP were (11.81∓1.28) mm, (2.49∓0.57) mm, and (1.90∓0.61) mm, respectively,which were not significantly different between male and female patients (P>0.05). In addition, the length, width, and height of IFP were significantly larger in nasorostral hypertrophy group than in normal group (P0.05).

CONCLUSIONUltrasonography can accurately evaluate the size and morphology of IFP pre-operatively, and therefore is helpful to ensure the success of rhinoplasty.

Adipose Tissue ; anatomy & histology ; diagnostic imaging ; Adolescent ; Adult ; Female ; Humans ; Male ; Nose ; anatomy & histology ; diagnostic imaging ; surgery ; Preoperative Care ; Ultrasonography ; Young Adult

OBJECTIVETo study the effects of different parameters (frequency, intensity, needle-retained time and treatment interval) of electroacupuncture at Fenglong (ST 40) for adjusting blood lipids, so as to find out the optimization parameter.

METHODSFifty-four cases meeting the criteria for hyperlipoidemia were randomly divided into 27 groups with orthogonal design L27 (3(13) ). According to the orthogonal design program they were treated with electroacupuncture at Fenglong (ST 40). Ten sessions constituted one course with a one week's interval between two course. The treatment was given for 2 courses.

RESULTS(1) The parameters of EA at Fenglong (ST 40) for regulating blood lipids in primary and secondary orders are: frequency, needle-retained time, interval of treatment, intensity. (2) The parameters of EA at Fenglong (ST 40) for various programs in regulating various blood lipids are: for TG, frequency AM 50 Hz, needle-retained time 20 ain, intensity 1 mA, twice each week; for TC, frequency AM 100 Hz, needle-retained time 30 min, intensity 1 mA, once every other day; for LDL-C, frequency Am 100 Hz, needle-retained time 30 min, intensity tolerable and comfortable, once every other day.

Acupuncture Points ; Aged ; Cholesterol ; blood ; Cholesterol, HDL ; blood ; Cholesterol, LDL ; blood ; Electroacupuncture ; methods ; Female ; Humans ; Hyperlipidemias ; blood ; therapy ; Lipids ; blood ; Male ; Middle Aged

To establish a quality control method of Dendrobium aurantiacum eye drops, in order to evaluate acute toxicity, irritation and irritability and lay a foundation for its development and utilization in the future. The content of gigantol and SA in D. aurantiacum eye drops were determined by high-performance liquid chromatography (HPLC). The linear ranges of gigantol and SA were 0.040 8-1.530 0 g x L(-1) (r = 0.999 9) and 0.100 8-0.504 0 g x L(-1) (r = 0.999 9), with the average recoveries being 100.8%, 99.84%, and RSD being 1.4%, 1.8% (n = 9) respectively. The sample solution was stable at room temperature within 72 h. The acute toxicity test showed no toxic reaction of D. aurantiacum eye drops in mice. The irritating test for single-dose and multiple-dose administrations of D. aurantiacum eye drops and physiological saline in rabbit eyes and skin, as well as the allergic test in guinea pigs showed no eye irritation and skin irritation and irritability. These findings indicated that D. aurantiacum eye drops are safe and stable, with a good druggability.
Animals ; Cataract ; drug therapy ; Dendrobium ; chemistry ; Diabetes Complications ; drug therapy ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; standards ; Eye ; drug effects ; Female ; Guinea Pigs ; Humans ; Male ; Mice ; Ophthalmic Solutions ; administration & dosage ; adverse effects ; standards ; Quality Control ; Rabbits ; Skin ; drug effects

OBJECTIVETo report a method of repair facial skin defects with a skin flap of SMAS pedicle.

METHODSAccording to the size of defect of skin, design a skin flap with SMAS pedicle for repair of defect.

RESULTSThe method has been successfully applied for skin defects of eyelid and lip in 14 cases with satisfied results. The area of the largest flap was 5 cm x 3 cm.

CONCLUSIONRepairing facial defects such as eyelid skin defect or lip skin defect with skin flap of SMAS pedicle is a very good method. The flap has a good blood supporting and satisfactory color and flexibility.

Adolescent ; Adult ; Child ; Child, Preschool ; Facial Dermatoses ; surgery ; Facial Injuries ; surgery ; Humans ; Male ; Subcutaneous Tissue ; transplantation ; Surgical Flaps ; Young Adult

OBJECTIVETo search for a simple and non-invasive method to assist the treatment of the complications of breast augmentation with polyacrylamide hydrogel injection.

METHODSHigh-frequency ultrasound was used to examine the breast and observe the distributions of the injected polyacrylamide hydrogel. The operation procedure was predetermined according to the ultrasound information. The ultrasound results were compared with what was seen during the operation.

RESULTS40 patients (80 breasts) were examined. The ultrasound results were coincident with the outcomes of surgery. The results of postoperative follow-up were coincident with the predicted.

CONCLUSIONUltrasound is an accurate method for examining the augmented breast with polyacrylamide hydrogel injection. It is helpful in predetermining the operation procedure, predicting postoperative results.

Acrylic Resins ; adverse effects ; Adult ; Breast ; pathology ; surgery ; Female ; Humans ; Injections ; Surgery, Plastic ; adverse effects ; Treatment Outcome ; Ultrasonography, Mammary

OBJECTIVETo explore the relationship between interleukin-6 (IL-6) (-634C/G) genetic polymorphisms and the pneumoconiosis.

METHODSA total of 104 male stage I pneumoconiosis patients diagnosed by the Pneumoconiosis Diagnosis Expert Panel according to the Chinese National Diagnosis Criteria of Pneumoconiosis (GBZ 70 - 2002) were selected. The pneumoconiosis comprised 66 silicosis and 38 coal worker' pneumoconiosis (CWP). A total of 122 workers exposed to same dusts as the patients but without pneumoconiosis including 77 exposed to silica dusts and 45 to coal dusts were selected. The patients and the controls had the same dust exposure history. The peripheral venous blood was drawn from each subject. The IL-6 (-634C/G) genetic polymorphisms were detected by polymerase chain reaction restriction fragment length polymorphisms (PCR-RFLP) techniques.

RESULTSThe frequencies of IL-6 (-634C/G) (CC, CG and GG) genotypes were 66.7%, 19.7% and 13.6% in silicosis group, 42.9%, 42.9% and 14.2% in silica dust exposure group, 73.7%, 18.4% and 7.9% in CWP group, 51.1%, 35.6% and 13.3% in coal dust exposure group respectively. The statistical analysis indicated that there was significant difference in the frequencies of IL-6 (-634C/G) (CC, CG and GG) genotypes between silicosis patients and workers exposed to silica dusts (P < 0.05).

CONCLUSIONIL-6 (-634 C/G) genetic polymorphisms might play a role in the occurrence of silicosis.

Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Interleukin-6 ; genetics ; Male ; Matched-Pair Analysis ; Middle Aged ; Pneumoconiosis ; genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length

Objective To investigate the feasibility of self-made capacitation liquid for in vitro fertilization of Mongolian gerbils, and to provide a reference for gerbil embryo cryopreservation. Methods In vitro fertilization of Mongo?lian gerbil was performed with the self?prepared capacitation solution and semen, and the 2?cell embryos of Mongolian ger?bils were cultured in vitro using an improved KSOM culture medium. Results The in vitro fertilization rate of gerbils was over 60%, and some gerbil 2?cell embryos could develop further in vitro. Conclusions An in vitro fertilization and embry?o development system of Mongolian gerbil has been established, but it needs further optimization.