Objective To investigate the feasibility,safety and clinical efficacy of transvaginal myomectomy.Methods A total of 92 patients with uterine leiomyoma were given either vaginal myomectomy(Study Group,n=46) or abdominal myomectomy(Control Group,n=46).Clinical outcomes between the two groups were compared.Results Both vaginal and abdominal myomectomy were performed successfully.The operation time between the two groups was not statistically significant(t=-0.734,P=0.465).There were significant differences between the Study Group and the Control Group in the intraoperative blood loss(60.4(?5.6 ml) vs 82.5?50.2 ml;t=-2.210,P=0.000),the time to first passing flatus(15.3?3.2 h vs 27.5?4.8 h;(t=-14.343,)P=0.030),the incidence of postoperative pain (?~2=29.447,P=0.000),the rate of postoperative pyrexia(?~2=3.903,P=0.048),and the hospital stay(3.1?0.4 d vs 5.2?1.1 d;t=-12.169,P=0.000).Conclusions Transvaginal myomectomy has advantages of little invasion,quick recovery of bowel movement,slight postoperative pain,low rate of postoperative pyrexia,and short hospital stay,being a safe and feasible minimally invasive option for uterine leiomyoma.

Purpose:Evaluation of technique and clinical value of CT-guided transthoracic punc ture biopsy.Materials and methods:Between Feb 1994 and Oct 1997,CT guided transthoracic puncture biopsy was performed in 55cases including unknown pulmonary(44cases),pleural(6 cades)and mediastinal (6 cases)diseases with 20gDFBN and 18g ADGSTN.36 males and 19 females,aged from 23 to 78 years (mean age 55 years)were included.The assembled materials were evaluated cytologically in 25 cases and histopathologically in 55 cases.Results:The needle tip passed through percutaneouslly into the lesion under CT-guided in all 55 cases and obtained specimens for cytology(25 cases)and histopathology(55 cases).Accuracy rate were 60% for cytology and 87.5% for histopathology.Among 35 cases of tumor, histopathologic classification had been done in 26 cases.Complication occurred in 7cases(13%),including pneumothorax(2 cases)and hemoptysis(5 cases).Conclusion:CT-guided TPB is a method of combining advanced imaging tecnique and pathology.It is a simple,highly accurate,safe diagnostic tool,especially for unknown pulmonary,pleura,mediastinal lesions.

Antigens ; analysis ; Microarray Analysis ; methods ; Quantum Dots ; Semiconductors

Objective To explore the effect of Fas/FasL pathway on fluoride.induced apoptosis in hurnan neumbla8toma SH-SY5Y cells.Methods The cell survival rate,percentage of apoptosis,and mRNA expression levels of Fas and FasL were measured respectively after the SH-SY5Y cells were exposed to O(control),20,40,80 mg/L sodium nuoride(NaF)for 24 hours/n vitro.Furthermore,the changes of the percentage of apoptosis and mRNA expression levels of Fas and FasL in 40 mg/L NaF-treated groups incubated with activaling or neutralizing anti-Fas antibody(CH11 or ZB4)also observed respectively.Results Compared with the control group(100.00%), the cell surval rates in 40,80 mg/L NaF-treated groups[(84.63±2.57)%,(69.04±5.63)%]were significandy lower(P<0.01).The percentage of apoptosis in 40,80 mg/L NaF.treated groups[(8.54±1.95)%.(17.94±2.71)%]were higher(P<0.05)than thal in the control group[(3.32±1.33)%],and increased with the dose of NaF.NaF could up-regulate Fas and FasL mRNA expression,and increased the Fas/β-actin [40 ms/L group (0.94±0.51),80 mg/L group(0.99±0.12)]and FasL/β-actin[40 mg/L group(0.96±0.42),80 mg/L group(0.99±0.24)] ratio,compared with the control[Eas/β-actin(0.50±0.33),FasL/β-actin(0.58±0.23)],both the difference had 8tatistical significances (P<0.05).NaF and CH I 1 had a synergisfic effect on apoptosis and mRNA expression levels of Fas and FasLL(F=32.89,18.46,.14.69,P<0.01)while NaF and ZB4 had an antagonistic effect (F=5.73,24.26,10.17,P<0.05 or<0.01).Conclusion NaF exposure can cause apoptosis in SH-Y5Y cells,and the Fas/FasL pmhway may play an important role in NaF-induced apoptosis.

Child ; Child, Preschool ; Endoscopy, Gastrointestinal ; methods ; Female ; Gastrointestinal Diseases ; diagnosis ; Humans ; Male ; Purpura, Schoenlein-Henoch ; complications ; diagnosis

Objective To explore the early diagnosis of Alport′s syndrome(AS).Methods Renal and skin biopsy was carried out in 5 patients who manifested with isolated hematuria and nephritic syndrome(NS).By using indirect immunofluorescence method,the expression of type Ⅳ collagen ? chains was detected on epidermal basement membrane(EBM) and glomerular basement membrane(GBM).Results ?_1 chains on EBM and GBM were expression in all patients,but ?_5 chains on EBM and ?_3,?_5 chains on GBM form 2 female patients were segmental expression.Thus the goal for early diagnosis was achieved.Conclusions ? chains for EBM type Ⅳ collagen and GBM type Ⅳ collegan should be investigated if condition permits for those patients with isolated hematuria,NS(steroid-resistant) and thinned GBM in electron microscopy.It can be useful for diagnosis and differenial diagnosis of AS.

OBJECTIVEDetection HBV DNA among HBsAg negative children who have been vaccined at birth, in order to improve the evaluation of the indicator for HBV DNA infection.

METHODSSelection HBsAg negative children who have been vaccined at birth and then detection HBV DNA from sera using QIAamp Viral DNA Mini Kit, HBV DNA s region was obtained by nested PCR and sequencing.

RESULTS12 of the 140 children were HBV DNA detected were positive and the infectious rate was 8.6% . No mutant of the 12 HBV DNA in "a" determinant.

CONCLUSIONTo evaluate the effection of the prevention of HBV mother-to-child transmission, the standard method should be established. The detection of HBV DNA should be included in the future.

Child ; Child, Preschool ; DNA, Viral ; blood ; genetics ; Female ; Hepatitis B ; blood ; prevention & control ; transmission ; Hepatitis B Surface Antigens ; blood ; genetics ; Hepatitis B virus ; genetics ; Humans ; Infectious Disease Transmission, Vertical ; prevention & control ; Male ; Mutation

Objective To evaluate the effects of δ-aminolaevulinic acid-photodynamic therapy (ALA-PDT) in the treatment of primary carcinomas on the facial skin. Methods In the accordance of these tumors' sites and morphology, 14 patients with squamous cell carcinoma (SCC), 38 patients with basal cell carcinoma (BCC) and 5 patients with Bowen disease were given four to eight times of topical ALA followed by PDT. Results Ten (71.4%) SCC cases, 34 (89.5%) BCC cases and all (100%) Bowen disease cases completely recovered after ALA-PDT. The others all obtained signifi-cant improvement after final treatment. Their unaffected tissues around these tumors kept well and no scaring appeared after ALA-PDT. The recurrence rates among the completely-recovered cases were 10.0% (SCC), 11.8% (BCC) and 0% (Bowen disease), respectively, by the end of six-month's follow-up. Conclusions Topical ALA-PDT is an effective new therapeutical method with lower recur-rence rates, fewer side effects, no scar formation and excellent cosmetic results for primary carcinomas localizing on the facial skin.

Objective To assess the value of integrated 18 F-fluorodeoxyglucose (FDG) PET/CT in differentiation of malignant and benign pericardial effusion. Methods 18F-FDG PET/CT were performed in 23 patients with pericardial effusion. The detected soft tissue tumor or nodulous lession in pericardium or the thickened pericardium, with the maximum standardized uptake value( SUVmax ) ≥2.5, was defined as PET/CT-positive. The invaded lession in pericardium with SUVmax ≥2.5 was also as the positive. The difference of SUVmax of benign and malignant lesions was analyzed with two-independent-sample test of nonparametric tests. The final diagnosis was confirmed by biopsy or post-operative pathology. Results The diagnosis were confirmed with 14 malignant and 9 benign lesions. The median of SUVmax was 6.0 in malignancy group and 2.2 in benign group (z= -3. 279, P =0.001 ). According to the pathology results, there were one false negative case and two false positive cases with PET/CT imaging interpretation. The sensitivity, specificity,accuracy, positive predictive value ( PPV ) and negative predictive value ( NPV ) of 18 F-FDG PET/CT in diagnosis of benignity or malignance of pericardium effusion were 92.9% ( 13/14), 7/9, 87.0% (20/23),86.7% (13/15) and 7/8, respectively. Conclusion For the patients with pericardium effusion 18F-FDG PET/CT may be a helpful modality for malignancy differentiation

Objective To explore the problems in the introducing of complementary food in infants and provide a guidance for cli nical practice.Methods The questionnaire survey was carried out by childhood health doctors in mothers or caregivers on fixed outpatient health care day. The data of survey was entered into computer and analyzed with SPSS 11.5 software.Results 1.Earlier (