Objective: To study the effect of metastasis suppressor gene Kail/CD82 on the cell proliferation of Hep-2 cell line. Methods: Recombinant adenovirus rAd-Kail was amplified, purified, and was used to transfect laryngo-carcinoma Hep-2 cell line. Cells transfected with blank vector and untransfected cells served as controls. MTT assay was used to assess the influence of Kail gene on proliferation of Hep-2 cells; RT-PCR was used to examine the expression of Kail gene in cells of different groups. Results: The titer of rAd-Kail reached 6 X 1010 PFU/ml after expansion and purification. When the concentration of the adenovirus was 30 MOI, the transfection rate of Hep-2 cells could be higher than 90%. MTT assay showed that the cell proliferation abilities were similar in the two control groups, and the proliferation ability of cells in the Kail transfection group was significantly lower than that in the non-transfected group (P<0. 05) ,with the inhibitory rate in the Kail group being 29%. Conclusion: The Kail/CD82 gene can inhibit the proliferation of Hep-2 cells.

Objective To explore the risk factors of lung cancer in rural area. Methods A 1∶1 matched case_control study was conducted in 106 individuals in Heilongjiang province. Results The amount of passive smoking (OR:2.48,95%CI:1.51～4.08),history of mental scar (OR:4.63,95%CI:1.51～14.15), smoking from Kang (OR:1.69,95%CI:1.10～2.59), smoking indexes (OR:1.75,95%CI:1.10～2.79) and chronic bronchitis (OR:4.67,95%CI:1.12～19.49) had a closely correlation with lung cancer in rural area.Conclusions Lung cancer might be caused by multiple factors synergetically.The main countermeasures for controlling lung cancer were to give up smoking and to improve the conditions of heatable brick bed and heating method.

Adolescent ; Humans ; Male ; Parasomnias

Streptolysin S (SLS),one of the important virulence factors of Streptococcus,exist in several kinds of human and animal pathogenic bacterial,including Streptococcus pyogenes,Strepstococcus iniae and Streptococcus anginosus.SLS is a peptide toxin encoded by nine consecutive genes (sagA-sagⅠ).The functions of SLS include contributing pathogenic bacterium to pass through epithelial barrier,causing tissue damage,resisting to phagocytic clearance of host immune cells and interacting with other virulence factors.In addition,SLS as a signaling molecule of cell quorum sensing is involved in regulating the expression with other virulence factors.This paper summarized the structures and the biological functions of SLS in Streptococcus infection.

A new caffeate compound, (E)-erythro-syringylglyceryl caffeate (1), was isolated from the roots and rhizomes of Nardostachys chinensis Batal., together with nine known phenolic compounds, including (+)-licarin A (2), naringenin 4', 7-dimethyl ether (3), pinoresinol-4-O-β-D-glucoside (4), caraphenol A (5), Z-miyabenol C (6), protocatechuic acid (7), caffeic acid (8), gallic acid (9) and vanillic acid (10). Their chemical structures were elucidated on the basis of spectroscopic data and physicochemical properties. Furthermore, this is the first report of compounds 2, 5 and 6 from Nardostachys genus.
Caffeic Acids ; chemistry ; isolation & purification ; Flavanones ; chemistry ; isolation & purification ; Furans ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Hydroxybenzoates ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Nardostachys ; chemistry ; Plant Roots ; chemistry ; Rhizome ; chemistry ; Vanillic Acid ; chemistry ; isolation & purification

Objective To analyze the association between mtDNA mutations and photodamagc after ultraviolet B (UVB) irradiation. Methods Primary human skin fibroblasts (HSF) and primary human epi- dermal keratinocytes of adult (HEKa) were irradiated by sub-lethal doses of UVB thrice a day for 4-5 days. Thereafter, genomic DNA was extracted from irradiated cells and conventional PCR was applied to detect the frequency rates of 4977 bp and 3895 bp mtDNA deletion. To quantitatively analyze the mutation levels, SYBR Green real-time PCR method was performed. Results In both cell lines, the frequency rates and relative copy number of deletions increased with the cumulative doses of UVB exposure (P<0.05). The prevalence rate of 3895 bp deletion peaked 53.3% and and relative copy number reached (49.63±4.38)×10-5, showing a more intense response to the accumulation of UVB radiation than 4977 bp deletion. In HSF, the minimum cumu- lative dose of UVB radiation was 150 mJ/cm2 for the induction of 3895 bp deletion, and 200 mJ/cm2 for the induction of 4977 bp deletion. It seemed that mtDNA deletion was more readily to be induced by UVB radia- tion in HSF than in HEKa. Conclusions The development and accumulation of mtDNA mutation are intimately related with cumulated UVB dose received by skin cells, and the 3895 bp deletion is more reliable in moni- toring the photodamage caused by UV than 4977 bp deletion. Therefore, the 3895 bp deletion may serve as a biomarker for the detection of photodamagc in skin cells. HSF appear to have an increased susceptibility to UVB radiation, which results in a higher frequency and level of mtDNA mutations compared with HEKa.

Against the deficiencies of the current preventive medicine education curriculum,training programs and curriculum were reformed according to the training objectives of the modem preventive medicine, while new ideas of preventive medical personnel training were explored in order to cultivate practical professionals with a certain ability to innovate and to adapt the development of modern public health and preventive medicine preferably.

Objective To understand employment status of the eight-year program medical graduates from different medical colleges and put forward reasonable proposals on the current prob-lems. Method Through questionnaire, we conducted research among eight-year program medical graduates of three different grades (Grade 04,05,06) on the general condition of employment, research level, actual working time, expectations and actual salary, satisfaction with jobs and so on. Data statis-tics was done by Excel 2007. Totally, our research involved 11 medical colleges and 389 questionnaires. Results 94.34% (367/389) of the eight-year program medical graduates work in top three hospitals. The top five factors affecting the employment were reputation of the school 86.38%(336/389), reputation of the Principal Investigator 66.83% (260/389), social relations 60.15% (234/389), scientific research level 52.96% (206/389) and gender 46.79% (182/389). The amount of published papers from those graduates and their satisfaction with jobs vary greatly. Conclusion Eight-year program medical grad-uates employment faces both opportunities and challenges, and a clear training objective and improve-ment of continuing medical education system for the eight-year program medical graduates will have great significance on the promotion of their employment.

Objective To discuss the application effect of LVIS stent-assisted coil embolization in intracranial wide-neck small aneurysms.Methods The clinical data of 32 patients with intracranial wide-neck small aneurysms treated with stent-assisted coil embolization were analyzed retrospectively and evaluated.Results In 32 cases,the embolization grade was Raymond Ⅰ in 23 cases,Raymond grade Ⅱ in 7 cases,and Raymond Ⅲ in 2 cases.The successful embolization rate was 93.8%.No aneurysm was ruptured during embolization.One patient had thrombotic event in the surgery,but the occluded artery was recanalized after the treatment of immediate thrombolysis.Disorder of contralateral limb's activity and confusion were appeared in two patients after surgery.The symptoms improved by giving clopidogrel,aspirin and increasing the dosage of nimodipine.All the survival patients recovered well after 6 months of follow-up.No recurrence of aneurysm was confirmed by DSA and parent artery was patent.Conclusion Lvis stent is simple to be operated and has various models,suitable for most intracranial aneurysms,showing good performance.It suits embolization therapy in intracranial wide-neck small aneurysms,but the strong thrombosis should be valued.

OBJECTIVE:To improve the determination method for the contents of main components and related substances in Loxoprofen sodium tablets. METHODS:RP-HPLC method was adopted. The determination was performed on Inspire C18 column with mobile phase consisted of acetonitile-0.01 mol/L potassium dihydrogen phosphate(containing 0.2% triethylamine,phosphoric acid adjusted to 3.0±0.1,62 : 38,V/V)at the flow rate of 1.0 mL/min. The column temperature was 40 ℃,and the detection wave-length was set at 221 nm. The sample size was 20 μL. RESULTS:The peak of loxoprofen sodium was well separated with the peak of its related substances(R>1.5). The linear range of loxoprofen sodium ranged 30.0-90.0 μg/mL(r=0.9998). The detection lim-it of loxoprofen was 0.3 μ g/mL. RSDs of precision,stability and repeatability tests were <1.0% . The average recovery rates ranged 99.00%-99.87%(RSD=0.33%,n=9). CONCLUSIONS:This method is accurate,simple,rapid and suitable for the quali-ty control of Loxoprofen sodium tablets.