Due to influnce of various factors, the transplanted kidney will appear the chronic renal insufficiency symptom at a certain period after transplantation. How to prolong the function of transplanted kidney and life of the patients is a key subject in clinical research. This study based on the retrospective analysis of 22 cases kidney transplant of chronic kidney failure patients enrolled in the Department of Blood Purification, Hainan SanYa Nongken Hospital. According to the different stages of the chronic renal failure of patients, tacrolimus (FK506) was used instead of cyclosporine A (CsA) combined with blood purification treatment (hemodialysis or/and hemoadsorption), the abnormal biochemical markers decreased significantly in all patients after treatment, with 7 cases are clinical cured (7/22, 31.8%), 10 cases are ameliorated (10/22, 45.5%) and 5 cases gave up treating (5/22, 22.7%). The result indicates that this therapy can ameliorate the transplanted kidney function, and improve the life quality of the patients.

Objective To explore the relationship of apolipoprotein E(ApoE) and its gene polymorphism with phlegm and blood stasis syndrome in patients with coronary heart disease(CHD).Methods Ninety-seven qualified CHD patients involving phlegm syndrome(PS),blood-stasis syndrome(BSS),and phlegm and blood stasis syndrome(PBSS) were adopted.Thirty-five healthy volunteers were enrolled into the control group.Serum ApoE level was examined in all of the subjects,and their whole blood DNA were extracted for the detection of ApoE gene polymorphism by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Correlation analysis of the data was performed with SPSS11.0 software.Results The difference of diabetes mellitus,smoking,low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) was significant between CHD patients and healthy volunteers(P

Objective To explore the methods of preparing whole blood control of seven trace elements (magnesium,manganese,iron,copper,zinc,lead,calcium) in laboratory and evaluate its performance.Methods Heparin sodium anticoagulant calf whole blood was used as substrateMetal salt or standard solution with target concentration of each element was added.And whole blood control product was made after process of anticorrosion,mixing and sub-packaging.Antibacterial effect was observed,uniformity and stabilitywasevaluatedaccording to CNAS-GL03 and matrix effects was evaluatedaccording to CLSI EP14.SDI (standard deviation index) and detection coefficient of variation (CV)were calculated to evaluateapplication effectiveness.Results Laboratory preparation of whole blood control reached target concentration,sterility tests was qualified,results of uniformity and stability indicated that the substrate was even and stable at least for one year.Besides,matrix effects of other six elements can be ignored except lead.Historical and inter-laboratory comparisons had shown that laboratory preparation of whole blood control has no obvious difference with commercial ones in performance.Conclusion The formulation and evaluation scheme of whole blood control of seven trace elements (magnesium,manganese,iron,copper,zinc,lead,calcium) was feasible and can be used as commercial ones for elementary tests in medical laboratory.

Objective To explore the changes and clinical significance of prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT),fibrinogen time(FGB)and platelet(PLT)on Kawasaki disease(KD)in children with acute and convalescent 10 d,which aimed at early diagnosis,prediction and prognosis of coronary artery lesions.Methods Thirty-eight cases who were diagnosed KD were selected as KD group,30 cases age-matched acute respiratory infections in children with fever as fever group,moreover,30 cases of a class of elective surgery preoperative children admitted to surgical departments were put as control group.The plasma PT,APTT,TT,FGB,PLT of all cases and plasma APTT,FGB,PLT in recovery 10 d in children with KD disease were detected,and then the results were compared between the 3 groups;and the results of APTT,FGB,PLT in KD children with acute and convalescent 10 d to coronary artery dilatation groups or not were compared.Results 1.APTT prolonged and FGB,PLT increased in KD children with acute stage,which had a significant difference compared with other groups(Pa0.05).2.When comparing the results of APTT,FGB,PLT in KD children with acute and convalescent 10 d,the difference was significant(Pa

This paper was mainly on pesticide degrading microorganisms. The major pesticide degrading bacteria were Pseudomonas, Bacillus, Flavobacterium, Alcaligenes, Arthrobacter, etc. The major pesticide degrading fungi were Aspergillus,Pinicielium, Rhizopus, Trichoderma, Fusarium, etc. The major pesticide degrading actinomycetes were Nocardia, Streptomyces, etc.

Objective To explore the characteristics of gross motor development in 1～6-year-old children with cerebral palsy at different levels. Methods 708 children (487 males and 221 females, age range: 1～6 years, from 6 rehabilitation centers in Shanghai) with cerebral palsy (CP) were assessed with Chinese version of Gross Motor Function Classification System （GMFCS） and Gross Motor Function Measure (GMFM). Distributions of GMFM scores at different GMFCS levels in children with cerebral palsy were analyzed. Results The GMFM-66 scores increased most in children with GMFCS Level Ⅰ, and more than 75% of them would be greater than 67 points in GMFM-66 score after the age of 48～50 months. The children with GMFCS Level Ⅱ～Ⅳ appeared similar increasing range of GMFM-66 scores in 1～6 years old. Less than 25% of the children at GMFCS Level Ⅱ would be greater than 67 points in GMFM-66 score before the age of 6 years, more than 50% of those at GMFCS Level Ⅲ would be less than 56 points, more than 75% of those at GMFCS Level Ⅳ couldn't exceed 46 points （except groups of 54～56 months and 66～68 months）. Compared with the children with other GMFCS levels, the GMFM-66 scores were always at very low level in children with GMFCS Level V, and trended to decrease with time after 5 years old. Conclusion The characteristics of gross motor development are different in children with cerebral palsy at different GMFCS levels.

Objective To explore the predictive value of Kinetics of white blood cell (WBC) elimination following induction chemotherapy for eider with acute myeloid leukemia(AML). Methods 71 elder with AML were reviewed. Chi-square and the Kaplan-Meier methods were used to identify the relationship between the nadir WBC count and time to WBC nadir with efficacy and survival. Results 28 patients (39.44%) achieved a complete re-mission(CR),another 19 (26.76%) had a partial remission,17(23.94%) patients had a non-remission,and 7 pa-tients(9.86%)died. Overall survival over 3 years was about 11.27% (8 cases),over 2 years about 23.94 % (17 cases),and over 1 years about 47.89 % (34 cases). The low WBC nadir and high WBC nadir in the bone marrow CR (9.86% and 12.68%)and the total survival rate (8.45% and 11.27%)were lower than the median absolute WBC nadir (16.90%, 15.49%), but were no statistical discrepancy (χ23.32,1.22, P=0.77, 0.54). The pa-tients who achieved WBC nadir in less than or equal to 10 days in the bone marrow CR(12.68%) and the total sur-vival rate(8.45%) were statistical significantly higher than those achieved it in greater than 10 days(26.76%). The patients whose WBC attained at low level less than or equal to 3 days in the bone marrow CR(16.90%) and the total survival rate(12.68%) were statistical significantly higher than those greater than 3 days (22.54%) (χ2 15, 57,11.71,4.85,9.54,P=0.001,0.01,0.03,0.04). Conclusion WBC nadir in loss than or equal to 10 days and WBC attained at low level greater than 3 days may serve as a worse prognosis.

OBJECTIVETo study the basic law of circulation of channels and collaterals.

METHODSInherit and develop ripe experiences of predecessors based on The Yellow Emperor's Internal Classic and other classic medical books.

CONCLUSIONCirculation of channels and collaterals has the eight laws, including naming law, distribution law, converging law, exterior-interior association law, beginning-ending running law, meridian-qi bidirectional circulation law, zang- and fu-organ pathway liaison law, and liaison law of connecting with trunks and sense organs.

OBJECTIVETo analyze and evaluate practical significance of the ebb-flow theory of ying-qi.

METHODSBased on The Yellow Emperior's Internal Classic, the comparisons of the number of channels, collaterals and their bone-length measurement participating in ebb-flow of ying-qi, ying-qi ebb-flow theory with the practically respiratory-pulse ratio, human heart rate and pulse rate, human respiratory times, ying-qi ebb-flow theory with early channel theory, ying-qi ebb-flow theory with degrees of whole day, were tested and verified.

CONCLUSIONPractically, the ying-qi ebb-flow theory conforms few to human body, which possibly is an assumption of the ancients.