1.Application of p504S、CK34?E12、p63 in the differential diagnosis of benign and malignant lesions of the prostate
Hong DU ; Wen LI ; Yanxiao LIANG
Chinese Journal of Postgraduates of Medicine 2006;0(12):-
Objective To evaluate the value of p504S,CK34?E12 and p63 immunostaining for the diagnosis of benign and malignant lesions of the prostate. Methods Expression of p504S、CK34?E12 and p63 in benign and malignant lesions of the prostate was observed by microwave SP immunohistochemical method. Results Continuous positive expression of CK34?E12 and p63 was found in the acini and ducts of most benign prostatic hyperplasia, and few cases showed interrupted expression, but p504S staining presented negative reaction; Interrupted expression of CK34?E12 and p63 was found in high prostatic intraepithelial neoplasia cases, and was weekly positive or negative for p504S; CK34?E12 and p63 staining was positive in five atypical adenomatous hyperplasia cases, one case presented focally positive reaction by p504S; CK34?E12 and p63 staining was negative in forty prostatic carcinoma, but positive by p504S;The gleason histologic grading of prostate carcinoma was negatively related to expression of p504S. Conclusion Combined detection of p504S、CK34?E12 and p63 can be most helpful for diagnosis of prostate carcinoma, especially in needle biopsy specimen.
2.Neonatal arrhythmia in two cases.
Fang LIANG ; Hong-bin JIANG ; Wen-xin ZHOU
Chinese Journal of Pediatrics 2007;45(11):879-880
3.The relationship between NDRG1 gene and differentiation level and metastasis in prostate cancer
Wen LI ; Hong DU ; Yanxiao LIANG ; Guorong LIU
Journal of Chinese Physician 2008;10(4):457-459
Objective To investigate the expression of NDRG1 and its relationship to differentiation level and metastasis in prostate cancer. Methods The expression NDRG1 was examined by immunohistochemistry LSAB method in 86 samples of prostate cancer and benigh prostate hyperplasia. Results The NDRG1 was shown to be highly expressed in 30(73.3%)of prostate cancer and 70.0%of benign prostate hyperplasia.There were no evident difference between prostate cancer and prestate hyperplasia(P>0.05).The expression of NDRG1 was gradually reduction with the Gleason score from 2-4 to 5-7 to 8-10 in prostate cancer, which was statistical significance(P<0.05).The expression of NDRG1 in lymph node or bone metastasis was significantly lower than that in no metastasis of prostate cancer. There was a negative correlation between the expression of NDRG1 and lymph node or bone metastasis. The age had no correlation with the expression of NDRG1(P>0.05).Conclusion NDRG1 is involved in the genesis and development of prostate cancer, which may be related to the differentiation level of prostate cancer and serve as a useful marker for early diagnosis of cancer and metastasis.
5.Irbesartan suppresses expression of VCAM-1 and ICAM-1 in myocardial ischemia-reperfusion areas in rabbits
Xingsan LI ; Yufeng CHEN ; Hong WEN ; Daoye LIANG
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To investigate the effects of irbesartan on acute myocardial ischemia-reperfusion injury and on expression of ICAM-1 and VCAM-1 in myocardial ischemia-reperfusion areas in rabbits. METHODS: Anterior descending branch of left coronary artery (LAD) was ligated in ischemia-reperfusion (IR) group but not in the sham group. Rabbits of IR group were subjected to 60 min of LAD occlusion, and 360 min of reperfusion. In the treated group, the rabbits were given irbesartan until the trial ended. Then, all rabbits were killed and tissue samples were removed from IR areas. After disposed with routine histological methods and stained with hematoxylin and eosin (HE), these samples were examined by light microscopy. The expression of ICAM-1 and VCAM-1 were also investigated by employing immunohistochemical SP method in the tissue samples. RESULTS: Degree of damage in cardiocytes and of neutrophil infiltration were more serious in IR group than those in sham group (P
6. Impurity profile study of cloxacillin sodium for injection
Chinese Pharmaceutical Journal 2016;51(6):488-493
OBJECTIVE: To investigate the impurity profile of cloxacillin sodium for injection and study the source of major impurities. METHODS: The impurities in samples from seven manufacturers were profiled by an optimized reversed-phase HPLC method and a newly established normal-phase HPLC method. The major impurities were prepared by semi preparation technology and identified by LC-QTOF-MS, 1H-NMR and 13C-NMR. RESULTS: Two new impurities relating with the salt-forming reaction and the purification process respectively, and four other new impurities were all successfully identified in this study. CONCLUSION: The study is suitable for profiling the impurities in cloxacillin sodium for injection and is quite useful for enhancing the quality control.
7.Quantitative determination of aqueous flare and cells in healthy eyes
Hong WANG ; Zeng-Chao ZHOU ; Wen-Bin WEI ; Liang LIANG ; Bin HOU ; Hai-Ting CHEN ;
Ophthalmology in China 1993;0(03):-
Objective To quantify aqueous flare and cells in the eyes of healthy subjects and to evaluate the effect of age and sex on the blood aqueous barrier. Design Prospective case series. Participants Four hundred and forty-two eyes of 221 healthy sub- jects. Methods Aqueous flare and cells of 442 eyes were evaluated with FC-2000 laser flare cell meter (LFCM). Main Outcome Mea- sures Aqueous flare and cells. Results The mean flare values of all of eyes was 4.7?2.9 pc/ms, it was 3.1 pc/ms in the age group of less than 10 years, 3.8 pc/ms in the age group of 40-49 years and 11.0 pc/ms in the age group of 80 years or over. The mean flare val- ues in the age groups of 50 years or over were significantly higher than that in the age group of 40-49 years (P
8.SELENIUM STATUS AND DIETARY SELENIUM CONTENT OF POPULATIONS IN THE ENDEMIC AND NON-ENDEMIC AREAS OF KESHAN DISEASE
Guang-Lu XU ; Wen-Lan XUE ; Pei-Yi ZHANG ; Chu-Fen FENG ; Shan-Yang HONG ; Wen-Sheng LIANG ;
Acta Nutrimenta Sinica 1956;0(03):-
By using whole blood selenium, 24 hr urinary selenium and hair selenium contents as the indices of assessing human selenium status, it was found that the populations in the endemic areas of Keshan disease were practically in a selenium poor status. The selenium contents in locally grown staple grains and daily diets in the endemic areas were also lower than those in the non-endemic areas. In an area covering a cross section of Keshan disease geographic belt in our country, the hair selenium contents of agricultural populations were measured. The results indicated that all the hair selenium contents in the endemic sites were always at a lower level, whereas those in the non-endemic sites distant from the endemic areas were generally at a higher level; they decreased gradually until the endemic areas were reached; and finally, along the contiguous region of the endemic and non-endemic areas they were insignificantly different.The hair selenium contents among the agricultural populations were significantly lower than those among the non-agricultural ones in the same endemic areas. However, no regular correlation had been observed between the seasonal prevalence of Keshan disease and the variation of hair selenium contents in the same populations living in the same endemic sites.It is considered that the endemic areas of the disease seem to be a Se-deficiency belt, and Se-deficiency probably might be a pathogenic geo-gen in the prevalence of Keshan disease.
9.Chemical constituents of Indigofera pseudotinctoria.
China Journal of Chinese Materia Medica 2010;35(20):2708-2711
OBJECTIVETo study the chemical constituents in the roots of Indigofera pseudotinctoria.
METHODThe constituents were isolated by column chromatography and their structures were elucidated by spectroscopic methods.
RESULTFourteen compounds were isolated from the ethanol extract of the plant and identified as maackiain (1), 3beta-hydroxy-olean-9 (11), 12-diene (2), 12-oleanen-3,11-dione (3), 3beta-acetoxy-12-oleanen-11 -one (4), formononetin (5), formononetin-7-O-beta-D- glucoside (6), 7,4'-dihydroxy-3'-methoxy isoflavone (7), afromosin (8), genistein (9), calycosin-7-O-beta-D-glucoside (10), vicenin-2 (11), isoliquiritigenin (12), beta-sitosterol (13), and daucosterol (14).
CONCLUSIONAll compounds were isolated from the plant for the first time, eleven compounds (except 1, 13 and 14) were isolated from the genus Indigofera for the first time.
Indigofera ; chemistry ; Plant Extracts ; analysis
10.Changes in intraosseous pressure and bone blood flow of the distal femoral shaft after femoral medullary canal blocking with bone cement
Hongyu LI ; Hong AN ; Bin LIANG ; Rongzhu LI ; Wen TIAN ; Minke WEI
Chinese Journal of Tissue Engineering Research 2008;12(14):2785-2788
BACKGROUND: Implantation of artificial joint of bone cement can result in long-term blocking of recipient site medullary canal and blood vessel lesion, and lead to changes in intraosseous pressure and bone blood flow of distal femoral shaft.OBJECTIVE: To explore the changes in the intraosseous pressure and bone blood flow of distal femoral shaft after blockage of the proximal and middle femoral medullary canal by bone cement.DESIGN: Controlled observation.SETTING: People's Hospital of Guangxi Zhuang Autonomous Region.MATERIALS: The experiment was performed at the Experimental Animal Center of Chongqing Medical University between July 2002 and April 2003. Thirty-two healthy adult New Zealand rabbits were selected and randomly divided into model group (n=24) and control group (n=8). Polymethyl methacrylate (PMMA) TJ bone cement was provided by Tianjin Synthetic Materials Research Institute.METHODS: The rabbit model was established by infusing femoral medullary canal of left side with PMMA. The lateral greater trochanter of anesthetized rabbits were resected below the third trochanter through spatium intermusculare by posterior lateral femur approach, but the neck of femur was remained to expose intertrochanteric fossa and entry of medullary canal following by intramedullary reaming to 1/2 length of femur. The wound was washed repeatedly to remove the destroyed myeioid tissues, and was dried with gauze. Bone cement was prepared by manually stirring powder with solution at a ratio of 2:1, until dough shape formed. A small piece of dough-shaped bone cement was filled in middle femoral stenosis as cavity blocker. Ten minutes later, the solidified bone cement was re-blended until dough shape and implanted into medullary canal fully. When the bone cement was completely solidified, the incision was sutured. The 24-modeled rabbits were randomly divided into 4 subgroups according to the following observation time points (n=6): postoperative 0 day (T0),4th week (T4), 8th week (T8) and 16th week (T16).MAIN OUTCOME MEASURES: ①Physiological pressure-measuring instrument was used to detect and compare the intraosseous pressure of bilateral distal femoral medullary canal of the model and control groups. ②Radionuclide bone imaging was used to detect and compare the dynamic and static images of bilateral distal femoral of the rabbit models at different time points.RESULTS: Thirty-two rabbits were all included in final analysis with no loss. ①There were no significant differences in the intraosseous pressure between the distal femurs in normal rabbits (P>0.05); the pressure of the experimental side increased significantly compared with control side (P<0.01). In model group, there were no significant differences in the intraosseous pressure between the control sides of rabbits at different time points (P>0.05), but the experimental sides presented continuous high intraosseous pressure state. The pressure of T4 was higher than that of T16 (P<0.05); there were no significant differences between any other two time points (P>0.05). ②Compared with the dynamic and static images at different time-points, the counting of nuclide in the experimental sides was markedly lower than the control sides at T0 and T4 while higher at T8 and T16; the count reached the peak at T16. There were no significant differences in the nuclide counting ratio between T0 and T8, T0 and T16, T4 and T16, T8 and T16, T4 and T8, respectively except T0 and T4 (P<0.05). The dynamic and static radionuclide bone imaging showed the same changes.CONCLUSION: The intraosseous and intramedullary blood circulation is severely damaged after blocking of the proximal and middle femoral medullary canal with bone cement, resulting in local alterations of hymodynamics, a series of changes in intraosseous pressure and bone blood flow in the distal femur, which causes continuous and a long-term high intraosseous pressure.