Adult ; Aged ; Female ; Heart Neoplasms ; pathology ; Heart Valves ; pathology ; Humans ; Lymphangioma ; pathology ; Middle Aged

Cytokines ; metabolism ; Humans ; Interleukins ; metabolism ; Lung ; metabolism ; pathology ; Pneumoconiosis ; etiology ; metabolism ; Receptors, Platelet-Derived Growth Factor ; metabolism ; Transforming Growth Factor beta ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

Objective To observe the effects of repetitive transcranial magnetic stimulation (rTMS) on the expression of glial fibrillary acidic protein (GFAP) and OX-42 in hippocampus and dentate gyrus in rats.Methods The rats were treated with 1 Hz, 100 mT TMS 10 min once a day for 14 days, and then the expression of GFAP and OX-42 in hippocampus and dentate gyrus were investigated by ABC technique of immunohistochemistry.Results Compared with the control group, there were no significant difference in the expression of GFAP and OX-42 between the two groups.Conclusion rTMS using our parameters does not cause brain injury in rats.

Objective To investigate expression of recombinant adenovirus(rAV) vector-mediated report gene in kidney, and to seek for the best passway of rAV vector-mediated gene transfer in vivo.Methods Thirty Sprague-Dawley(SD) rats were divided into 5 groups randomly. rAV-GFP(1013viral particales?L-1) was injected through different ways. 2, 7 and 14 d after injection, GFP expression in left kidney, right kidney, liver and lung was evaluated by fluorescence microscope and computer software. Results GFP gene expressed in kidey and reached peak 7 d after injection expression. Conclusion rAV vector-mediated report gene can be transducted in kidney. The rAV-mediated transgene expression in kidney is a potential strategy in the treatment of renal diseases.

Objective:To investigate the association between polymorphisms of estrogen receptor ? (ER?) gene (rs3020444) and perimenopausal syndrome (PS) in patients with liver qi stagnation syndrome (LQSS). Metheds:The ER?T/C genotype in 100 patients of PS of LQSS type (PS-LQSS group),86 patients of PS of non-LSDS type (PS-non-LQSS group) and 100 healthy subjects (control) was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay. Results:Partitions of ?2 method showed that the higher prevalence of ER?-TT genotypes was noted in PS-LQSS group (?2=8.307,P=0.004),and the higher prevalence of T alleles was noted in PS-LQSS group (?2=7.129,P=0.008). and Multinomial logistic-regression indicated that the odds ratios (OR) of the ER?-TT genotypes vs TC/CC for PS-LQSS was 2.222 (95%CI:1.172-4.744,P=0.015) after adjusting for common miscellaneous factors. Conclusions:It was suggested that ER?-TT genotypes was significantly associated with PS-LQSS,and ESR?-TT may be one of the genes that contribute to PS-LQSS.

Objective To evaluate the training effect of Public health emergencies.Methods The testing materials from the training course on public health emergencies in longgang district of Shenzhen in 2009 were analyzed.Results Of all 162 students,98.2% considered the training course was completely important,97.3% students considered the training was very necessary,after training,80% students considered the master degree of related knowledge was improved,93.8% students considered the effect of training on the role of dealing with public health emergency training was very well or average.Conclusions Emergency response training for dealing with the public health emergencies is very effective and completely necessary.

A 27-peptide,a fragment of hepatitis delta antigen(HDAg),was synthesized and used to develop an ELISA method for ihe detection of anti-HD.It was found that positive anti-HD reaction occurred between the coated 27-peptide and a stored sample of serum which was known anti-HD positive.Absorption test revealed that the synthetic peptide competed with natural HDAg for anti-HD,suggesting that the peptide possessed the antigenicity similar to that of natural HDAg.The antigenicity of the synthetic peptide was quite specific wihtout cross reaction with normal human and mouse sera and with anti-HA.anti-HB and anti-HC sera.Among 300 blood donors,there was only 1 case(0.33%)anti-HD positive with an ALT level 2 times higher than normal.In 41 cases of non-B hepatitis and 52 cases of HAV hepatitis,none was anti-HD positive.In 211 cases of various types of HBV hepatitis,21 were(9.95%)anti-HD positive,among whom 2/82(2.5%)werehealthy HBV carriers,6/43(13.95%)were patients with a-cute icteric hepatitis,6/60(10.00%)were patients of chronic active hepatitis,4/18(22.20%)were patients of severe hepatitis,and 3/8(37.50%)were those with liver cirrhosis.These results were consistent with those in our previous reports.

AIM: To investigate the potential causes and management of the clusters of diffuse lamellar keratitis (DLK) after laser corneal refractive surgery.METHODS: The study enrolled 98 eyes (53 patients) complicated with DLK after receiving laser in situ keratomileusis (LASIK), FS-LASIK or small-incision lenticule extraction (SMILE) in our center from February 10th,2016 to February 22th,2016.They were given clinical classification treatments according to corneal layer inflammatory extent and then followed up after 1, 3, 5, 7, 10d and 1mo.RESULTS: The clusters of DLK occurred 5 times in the study period.The incidence and degree of DLK significantly decreased after changed the sterilization, surgical equipments, temperature and humidity of the operating room.There were 80 eyes (82%) had stage 1 DLK, 11 eyes (11%) had stage 2, 4 eyes (4%) had stage 3 and 3 eyes (3%) had stage 4.The incidence of DLK after FS-LASIK was 40% (79 eyes in 42 patients), that after LASIK assistant by Hastome keratome was 45% (10 eyes in 5 patients), that after SMILE was 20% (9 eyes in 6 patients).After intensive treatment, as glucocorticoid treatment and flap lifting flushing, all cases recovered within 1mo.CONCLUSION: The outbreak of DLK may be associated with the disposable item, flushing liquor, temperature and humidity of the operating room.Early diagnosis, prevention and treatment are the key of decreasing the incidence of DLK.

0.05).Conclusions The serum concentration of C_3 rises and the titer of AChRAb decreases in MG patients after treatment with glucocorticoid. There is no correlation between C_3 concentration and titer of AChRAb. The complement possibly cooperates with AChRAb in pathogenesis of MG.

Objective To explore the effect of ghrelin on insulin release of mouse pancreatic islet ?-cell line(NIT-1 cells) and its probable mechanism.Methods NIT-1 cells were incubated in high-glucose DMEM with ghrelin.Then,the media was sampled for the assay of insulin by RIA.The(mRNA) expressions of glucose transporter 2(GluT2),pancreatic-duodenal homeobox-1(PDX-1),inwardly rectifying potassium channel with two transmembrane regions(Kir6.2) and sulphonylurea receptor 1(SUR-1) in the cells were detected by using RT-PCR.The cell proliferation was determined by MTT assay.Results(1) 10~(-9)mol/L to 10~(-7)mol/L of ghrelin inhibited dose-dependently the high-glucose challenged insulin release of the NIT-1 cells.(2) The mRNA expression of Kir6.2,but not GluT2,PDX-1and SUR-1,was down-regulated by 10~(-7)mol/L of ghrelin.(3) Ghrelin had no effect on proliferation of the cells.Conclusions Ghrelin inhibits high-glucose induced insulin secretion of the islet ?-cells.This effect may be secondary to the down-regulation for the expression of Kir6.2,(a component) of ATP-sensitive potassium channel.