The recent development of process analytical chemistry(PAC) in the past years is reviewed. The content includes process measurement, sensor, chemometrics etc. The future of PAC is also discussed. 62 references are cited

Accidents, Occupational ; Acute Disease ; Aniline Compounds ; poisoning ; Humans ; Occupational Diseases

Objective To investigate the effect of pigment epithelium- derived factor (PEDF) on p38MAPK-CREB pathway and the expression of fibronectin (FN) in human mesangial cells (HMCs) cultured with high glucose. Methods HMCs were treated with different concentrations of glucose and the osmotic control respectively in the presence or absence of PEDF for 24 h:normal glucose (5.6 mmol/L),24.4 mmol/L mannitol,high glucose (30 mmol/L),high glucose+PEDF(30 mmol/L glucose with 10 nmol/L PEDF,40 nmol/L PEDF or 100 nmol/L PEDF).After samples were collected,the expression of phospho-p38MAPK (p-p38) and p-CREB was assessed by Western blotting,while FN mRNA and protein expression was assessed with RT-PCR and ELISA respectively. Results In contrast to normal glucose and mannitol treatments,the expression of p-p38MAPK,p-CREB and FN increased significantly in high glucose group (all P＜ 0.01).However,PEDF abolished the up-regulation of p-p38MAPK,p-CREB and FN induced by high glucose (all P＜0.05). Conclusion PEDF may inhibit fibrosis through P38MAPK-CREB pathway in diabetic nephropathy.

0.05).Conclusion TP may have protective effects on chronical hypoxia induced polycythemia in rats.

Adolescent ; Adult ; Female ; Histiocytic Necrotizing Lymphadenitis ; diagnosis ; therapy ; Humans ; Lymph Nodes ; Male ; Middle Aged ; Neck ; Young Adult

[Objective] To observe the sera immunoglobulin E(IgE) and Complement C3(C3)level changes in patients with chronic urticaria.[Methods] To detect sera IgE and Complement C3 content of 60 cases of chronic urticaria patients and 60 cases of healthy persons by chemiluminescence and Immunity Transmission Turbidity.At the same time,we tested sera IgE and Complement C3 content of both penetration needling and drug groups before and after treatment of 60 cases,and anaylysed the correlation of IgE and Complement C3.[Results]Higher level of sera IgE was detected in patients with chronic urticaria(P 0.05);the correlation of IgE and Complement C3 was negative in both groups.[Conclusions]Detecting Sera IgE and Complement C3 level have reference value in the diagnosis and treatment of chronic urticaria.

Objective To evaluate the effects of Laparoscopic cholecystectomy (LC) combined with intraoperative cholanggiography (IOC)and intraoperative endoscopic sphincterotomy (IOEST) for the treatment of cholecystocholedocolithiasis. Methods 106 cases with cholecystocholedocolithiasis diagnosed and treated by LC combined with IOC and IOEST were reviewed retrospectively. Results 60 40%(64/106)patients diagnosed cholelithiasis pre-LC were found choledocholithisis; LC combined with IOC and IOEST was successfully performed on 99 of 106 cases(93 40%),and their CBD stones were completely cleared in 98 of 99 cases(99 00%). Six cases(6 1%) were complicated with mild acute pancreatitis, 1 case(0 01%) with duodenal perforation,1 case(0 01%) with bile leakage, and 2 cases(0 02%) with stomach stasis. Conclusions LC combined with IOC and IOEST was a safe ,effective approach to diagnosis and minimally invasive treatment of cholecystocholedocholithiasis.

Objective To study the characteristics of infarct-related-artery (IRA) in acute high lateral wall myocardial infarction and to give clinical clue for deducing the location of coronary artery lesions. Methods Ninety-six patients with acute high lateral wall myocardial infarction (77 males, 19 females, mean age 57.3?10.7 years) were enrolled in the study. They all had infarction for the first time and were confirmed to have single vessel coronary artery disease by coronary angiography. The patients were divided into 3 groups according to the ECG changes at the onset of infarction. The first group (group A) was simple high lateral wall infartion (23 cases). The second one (group B) was high lateral with anterior wall infarction (58 cases, among them 27 cases with anteral-septal infarction and the other 31 with extensive anterior infarction). The third group (group C) was high lateral with lateral wall infarction (15 cases). Lesion characteristics of IRA were analyzed. Results The infarction-related lesions were mainly on left anterior descending arteries in group B (56/58), and on left circumflex in group C (13/15). But those in group A could be on either diagonal (12/23) or left circumflex (11/23). The difference of distribution of IRA among these groups was statistically significant (P

The experiments were carried out in mice to investigate the effects of acupuncturing Tsusanli (AT) on physical work capacity and neuroendocrino-immunological system. Mice were divided into four groups. The general health condition of the animals during the experimental period, the swimming duration, the content of hemoglobin and T, C, T/C, C 3, C 4 of alexin in the serum, the coefficients of spleen and thymus were observed. Results showed that AT could effectively improve the general health condition of the mice and strengthen the mice endurance as manifested by the prolonged swimming time,the increased content of hemoglobin and T, T/C, C 3, C 4 of alexin in the serum, and the higher coefficient of spleen and thymus gland. These indicated that AT was effective in elimination of fatigue and improving the endurance performance,showing the possibility of its appllication in the field of sports medicine.

Objective To investigate the relationship of nitric oxide with nitric oxide synthase system in fetal rat kidney and the mechanism of renal injury after acute intrauterine ischemia(hypoxia)/reperfusion ischemia(hypoxia)/reperfusion. Methods Wistar rats of 21 gestational days were selected and divided into seven groups according to the different ischemia(hypoxia)/reperfusion time. The level of nitric oxide and the expression of nitric oxide synthase system were detected by biochemistry, immunohistochemistry and RT PCR. Results The NO level was decreased gradually with extension of ischemia, which was significantly different from sham group ( P