1.Effect of formaldehyde inhalation on red blood cell number and the hemoglobin level in mice.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(3):142-142
Administration, Inhalation
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Animals
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Erythrocyte Count
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Erythrocytes
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drug effects
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Female
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Formaldehyde
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toxicity
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Hemoglobins
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analysis
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Male
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Mice
2.Neuroprotective effects of salidroside against focal celebral ischemia/reperfusion injury in rats
Wenfang LAI ; Xiaoqin ZHANG ; Haimian HONG ; Xiuli XIE ; Guizhu HONG
Chinese Pharmacological Bulletin 2015;(6):775-779,780
Aim To investigate the effect of Salidroside on the focal celebral ischemia/reperfusion injury in rats and its underlying mechanism. Methods Adult male Sprague-Dawley rats, weighing 260-300 g, were ran-domly divided into three groups: sham, MCAO, MCAO+salidroside ( Sal ) groups. The rats were sub-jected to local celebral ischemia reperfusion with su-
ture-occluded method. The rats of MCAO +Sal group were treated intraperitoneally with salidroside ( 50 mg ·kg-1 ) for 6 days. Neurological deficit testing was performed with Longa’ s Scale. The mRNA expressions of Neun,Nogo-A,and NgR were detected by RT-qPCR in ischemic brain. The protein expressions of Neun, NGF , BDNF , Nogo-A and NgR were determined by
Western blot. Results Compared with MCAO group, salidroside significantly improved the neurological defi-cit,promoted the expressions of Bcl-2,Neun,NGF,BD-NF, and inhibited the expressions of Nogo-A, NgR. Conclusion Salidroside can reduce neurological defi-cit, increase the number of Nissl’ s Body and the ex-pression of Neun, and protect rats against focal cele-
bral ischemia/reperfusion injury,which may be accom-plished by increasing the expressions of Bcl-2, NGF, BDNF, and inhibiting the expressions of Nogo-A, NgR.
3.Protective effects of salidroside in MCAO rats
Xiuli XIE ; Haimian HONG ; Wenfang LAI ; Xiaoqin ZHANG ; Guizhu HONG
Chinese Pharmacological Bulletin 2015;(10):1452-1457
Aim To investigate the neuroprotective effect of salidroside in MCAO rats. Methods The rats were subjected to middle cerebral artery occlusion with suture-occluded method, and the neurologic injury and infarct size of rats were evaluated. According to the gene chip detected, the protein expressions of caspase-3 , cleaved caspase-3 were determined and the mRNA expressions of IL-6, Vgf, Hba-a2, Hbb-b1, Hbb, CD44, Pnpla2, Slc6a5 and Slc5a7 were tested. Re-sults Compared with MCAO group, salidroside signif-icantly improved the neurological deficit, reduced the infarct sizes, inhibited the expressions of cleaved caspase-3 protein and IL-6 mRNA, promoted the ex-pressions of Vgf, Hba-a2, Hbb-b1, Hbb, and reduced the expressions of CD44 , Pnpla2 , Slc6 a5 and Slc5 a7 . Conclusions Salidroside can reduce the neurological deficit and infarct size, and protect rats against focal cerebral ischemia-reperfusion injury, which may be ac-complished by increasing the expressions of Vgf, Hba-a2, Hbb-b1, and Hbb, and decreasing the expressions of cleaved caspase-3 , IL-6 , CD44 , Pnpla2 , Slc6 a5 and Slc5 a7 .
5.Comparison of proximal femoral locking plate and proximal femoral intramedullary nail-spiral blade in minimally invasive internal fixation for elderly patients with intertrochanteric fracture
Lai CHEN ; Lin LIU ; Lingchao YE ; Huaxing HONG ; Deqing ZHANG
Chinese Journal of General Practitioners 2014;13(11):910-913
Objective To compare the proximal femoral locking plate (LPFP) and proximal femoral intramedullary nail-spiral blade (PFNA) in minimally invasive internal fixation for elderly patients with intertrochanteric fractures.Methods One hundred and thirty patients aged over 60 years with intertrochanteric fractures were treated with minimally invasive internal fixation from January 2010 to June 2013,among them LPFP was applied in 69 cases and PFNA in 61 cases.The operation time,the numbers of intraoperative X-ray fluoroscopy,intraoperative blood loss,postoperative flow and decreased postoperative hemoglobin,postoperative complications,medical expenses,fracture healing time,postoperative hip Hariss function score were documented and compared between two groups.Results There were no significant differences in intraoperative blood loss [(132 ± 95) and (110 ± 79) ml],postoperative draining [(44 ± 16) and (41 ± 17) ml],decreased postoperative hemoglobin,fracture healing time [(10.5 ± 1.0) and (10.4 ± 1.5) weeks] and postoperative hip Hariss function score (87 ± 10 and 90 ± 8) between two groups.In LPFP group the number of intraoperative X-ray fluoroscopy (8.1 ±0.8 and 8.6 ± 0.9),hospitalization expenses,postoperative complications (7/69 and 16/61) were less than those in PFNA group (P<0.05 or 0.01),however,the operation time was longer (P<0.01).Conclusion LPFP may be a better choice in internal fixation for elderly patients with intertrochanteric fractures.
6.Role of autophagy and apoptosis in tumor.
Jin-long TANG ; Hong-he ZHANG ; Mao-de LAI
Chinese Journal of Pathology 2012;41(8):573-576
Animals
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Antineoplastic Agents
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therapeutic use
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Apoptosis
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Apoptosis Regulatory Proteins
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metabolism
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Autophagy
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physiology
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Beclin-1
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Humans
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Membrane Proteins
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metabolism
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Neoplasms
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drug therapy
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metabolism
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pathology
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Signal Transduction
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TOR Serine-Threonine Kinases
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metabolism
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Tumor Suppressor Protein p53
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metabolism
7.Clinical value and the serum levels of soluble interleukin-2R and interleukin-6 in patients with Kawasaki disease
Hua HONG ; Jun DENG ; Lingying ZHANG ; Changning YE ; Deming LAI
Chinese Journal of Rheumatology 2001;0(05):-
Objective To explore the change of serum sIL-2R and IL-6 levels in patients with Kawasaki disease (KD) and its role in the pathogenesis. Methods The serum slL-2R and IL-6 were detected by sandwich ELISA. The serum hs-CRP level was measured by DADE BEHRING BN ProSpec. Results The serum levels of sIL-2R and hs-CRP in KD before and after high-dose intravenous gama globulin(IVIg) were significantly higher than in healthy controls (P
8.Effects of remifentanil post-conditioning on aquaporin-1 expression during myocardial ischemia-reperfusion injury in rats
Zhongmeng LAI ; Hong ZHENG ; Wenhua CHEN ; Liangcheng ZHANG ; Pengtao LIN
Chinese Journal of Anesthesiology 2014;34(9):1128-1130
Objective To evaluate the effects of remifentanil post-conditioning on aquaporin-1 (AQP-1) expression during myocardial ischemia-reperfusion (I/R) injury in rats.Methods Twenty-four male.SpragueDawley rats,weighing 250-300 g,were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (group S),group I/R,and remifentanil post-conditioning group (group RP).Myocardial I/R was induced by 45 min occlusion of left anterior descending branch of coronary artery followed by 24 h reperfusion.Remifentanil 10 μg· kg-1· min-1 was infused over 10 min starting from 10 min before reperfusion in group RP,while the equal volume of normal saline was given instead in S and I/R groups.At the end of reperfusion,all the rats were sacrificed and their myocardial specimens from left ventricles were obtained for microscopic examination of thepathological changes and for determination of AQP-1 mRNA (using real-time fluorescent quantitative PC R) and AQP-1 protein (by Western blot) expression in the ischemic area and myocardial water content.Results Compared with S group,myocardial water content was significantly increased in the other two groups,AQP-1 mRNA and protein expression was up-regulated in group I/R,and no significant change was found in AQP-1 mRNA and protein expression in RP group.Compared with I/R group,myocardial water content was significantly reduced,and AQP-1 mRNA and protein expression was down-regulated in RP group.Conclusion Remifentanil post-conditioning reduces myocardial I/R injury possibly through down-regulating AQP-1 expression in myocardial tissues of rats.
9.Comparison of early clinical effects between Activ C cervical disc replacement and anterior cervical discectomy and fusion for single-level cervical spondylosis.
Hong-ke LI ; Chang-jiang ZHANG ; Ming-jun WANG ; Xian-yu YANG ; Lai-hao LI
China Journal of Orthopaedics and Traumatology 2015;28(11):1026-1031
OBJECTIVETo compare the early clinical effects of Activ C cervical disc replacement (ACDR) and anterior cervical discectomy and fusion (ACDF) in treating single-level cervical spondylosis.
METHODSThe clinical data of 76 patients with single-level cervical spondylosis underwent surgery from July 2009 to September 2012 were retrospectively analyzed. Among them, 28 patients were treated with ACDR (ACDR group), including 18 males and 10 females, aged from 32 to 62 years old with an average of (45.2±6.2) years; and 48 patients were treated with ACDF (ACDF group), including 28 males and 20 females, aged from 33 to 60 years old with an average of (45.8±6.4) years. Visual analogue scale (VAS), Japanese Orthopedics Association (JOA) score, Short Form-36 (SF-36), imaging data were used to assess the clinical effects after operation.
RESULTSA total of 76 patients were followed up from 6 to 24 months with an average of 13.2 months. VAS of neck pain and brachialgia were improved in all patients after operation (P<0.05), there was no significant difference between two group (P>0.05). Somato-score and psycho-score of SF-36 of two groups were obviously increased (P<0.05), ACDR group was better than that of ACDF group (P<0.05). In ACDR group, there was no significant difference in the range of motion of surgical segments and adjacent segments between preoperative and postoperative (P>0.05); heterotopic ossification around the edge of vertebral body occurred in 1 case on the 6th month after operation, no fusion was found on the 1st year after operation. In ACDF group, the adjacent vertebral disease occurred in 1 case and the patient underwent the reoperation.
CONCLUSIONActiv C cervical disc replacement can reduce the degeneration of adjacent segments and its early outcomes for the treatment of single-level cervical spondylosis are satisfactory, but the long-term effects still need study.
Adult ; Cervical Vertebrae ; surgery ; Diskectomy ; methods ; Female ; Humans ; Male ; Middle Aged ; Spinal Fusion ; methods ; Spondylosis ; surgery ; Total Disc Replacement ; methods
10.Phylogenetic analysis for Fritillaria hupehensis: evidence from ITS, rpl16 and matK sequences.
Hong-wu LAI ; Yao-dong QI ; Hai-tao LIU ; Jiu-shi LIU ; Ben-gang ZHANG
China Journal of Chinese Materia Medica 2014;39(17):3269-3273
The systematic position of Fritillaria hupehensis has been in dispute. Phylogentic analyses were conducted on sequences of ITS, rpl16, matK sequences for species of F. hupehensis and allies. Lilium davidii was designed as outgroup. The analyses were performed using MP and ML methods. Conclusions could be achieved as follow. The topologies of MP and ML are consistent. The samples of F. hepehensis from different places form a supported clade with a strong bootstrap. And then form a strongly supported clade with F. anhuiensis, F. monantha. The results suggests that although F. hupehensis has a closet relation with the two ones, it exists some difference.
DNA, Plant
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chemistry
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genetics
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DNA, Ribosomal Spacer
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genetics
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Endoribonucleases
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genetics
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Fritillaria
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classification
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genetics
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Molecular Sequence Data
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Nucleotidyltransferases
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genetics
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Phylogeny
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Plant Leaves
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genetics
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Ribosomal Proteins
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genetics
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Sequence Analysis, DNA
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Species Specificity