Objective:To study the characteristic of energy metabolism in moderate and severe OSA before and after CPAP. Methods:The REE was measured before and after 5-8 week treatment by CPAP in OSA patients. Health staff of single obesity in the hospital were as control. Results:During sleep, the enengy-metabolism was increased significantly in OSA patients. After treatment by CPAP, it was decreased (P

Eighty-seven patients with hyperthyroidism were enrolled in a retrospective study,and 35.6％ of them also suffered from pulmonary hypertension(PH).Advanced age and systolic or diastolic dysfunction of left heart were risk factors in the mechanism of PH associated with hyperthyroidism (all P＜0.05).However,autoimmune disorder was found as a non-related factor in the mechanism of PH(P＞0.05).

Objective To investigate the relationship of intraoperative cerebral oxygen saturation(rSO_2 )monitored with near-infrared cerebral oximeter (INNOS 5100) and postoperative cognitive dysfunction in patientsoperated upon under isoflurane or sevoflurane anesthesia and to determine the critical rSO_2 value below whichpostoperative cognitive dysfunction may occur.Methods Sixty ASA Ⅰ- Ⅱ patients of both sexes aged 62-80 yr,weighing58-77 kg schedules for elective abdominal surgery or surgery on the low limb were divided into threegroups according to their levels of education: group Ⅰthe illierate and uneducated (n = 20); group Ⅱ primaryschool education (6yr education (n = 20). Each group was furtherdivided into isoflurane and sevoflurane subgroups (n = 10 in each subgroup). The patients were unpremedicated.Anesthesia was induced with intravenous atropine 0. 3mg, propofol 1 .0-1. 5 mg?kg~(-1), fentanyl 2-3?g?kg~(-1) andvecuronium o. 1-0.2 mg?kg~(-1) and maintained with isoflurane or sevoflurane inhalation (0.9-1. 1 MAC )supplemented with intermittent i.v. boluses of fentanyl and recorded before anesthesia (baseline), after O_2inhalation(T_1), after induction of anesthesia(T_2), after skin incision (T_3), during operation (T_4)and at the end ofsurgery (T_5). Mini-Mental State Examination (MMSE) was performed before anesthesia and 1,4, 8, 12 and 24 hafter surgery. BP, HR, ECG, SpO_2, P_(ET) CO_2 and end-tidal concentration of inhalational anesthetics werecontinuously monitored during anesthesia. Results In all three groups rSO_2 was significantly lower during operation(T_4) and at the end of surgery (T_5 ) than baseline (T_0 ) (P

Animals ; Birds ; Hemagglutinin Glycoproteins, Influenza Virus ; genetics ; immunology ; Humans ; Influenza A virus ; genetics ; immunology ; pathogenicity ; Influenza in Birds ; immunology ; pathology ; virology ; Influenza, Human ; immunology ; pathology ; virology ; Virulence ; genetics

Objective:To prepare and optimize sorafenib-Eudragit RS nanoparticles( S-E üPs)and investigate the physicochemi-cal properties. Methods:S-E üPs were prepared by a solvent-nonsolvent method. Single factor experiments were used to research the effect of solvent,stabilizer type,carrier ratio and the proportions of water phase and organic phase on the physicochemical properties of S-E üPs. S-E üPs were evaluated by the particle size,zeta potential and morphology,and the in vitro drug release of S-E üPs was studied using dialysis technology. Results:The mean size was(86. 72 ± 3. 71)nm,the PDI and zeta potential was(0. 20 ± 0. 032)and (36. 6 ± 0. 3)mV,respectively,S-E üPs showed spherical shape with uniform distribution. The drug release in vitro was accorded with a Weibull equation. Conclusion:The solvent-nonsolvent method is appropriate for the preparation of S-E üPs. The nanoparticles have small particle size,uniform distribution,regular morphology and significant sustained-release property.

Adipocytes ; cytology ; Cell Line ; Endothelial Cells ; cytology ; drug effects ; Epithelial-Mesenchymal Transition ; drug effects ; Humans ; Melatonin ; pharmacology ; Mesenchymal Stromal Cells ; cytology ; drug effects ; metabolism

Objective To investigate the effects of different depths of sedation during combined intravenous-inhalational anesthesia on postoperative cognitive function in patients undergoing gynecological laparoscopic surgery.Methods Ninety ASA Ⅰ or Ⅱ patients,aged 20-64 yr,with a body mass index of 19-30 kg/m2,scheduled for elective gynecological laparoscopic operation,were randomly divided into 3 groups (n =30 each).Anesthesia was induced with midazolam,fentanyl,propofol and rocuronium.The patients were tracheal intubated and mechanically ventilated.Anesthesia was maintained with inhalation of sevoflurane (end-tidal concentration 1.0％-1.5 ％),iv infusion of remifentanil and intermittent iv boluses of rocuronium.The infusion rate of remifentanil was adjusted to maintain BIS value:30 ＜ BIS value ≤ 40 in group Ⅰ,40 BIS value ≤ 50 in group Ⅱ and 50 ＜ BIS value≤60 in group Ⅲ.Cognitive function was assessed using Mini-Mental State Examination (MMSE) and TrailMaking Test (TMT) at 1 d before anesthesia and 1 d after surgery.Results MMSE scores were ＞ 24 at 1 d before anesthesia and 1 d after surgery in all the three groups,and there was no significant difference within each group and among the three groups (P ＞ 0.05).Compared with the baseline value,TMT completion time was significantly prolonged at 1 d after surgery in groups Ⅰ and Ⅲ,while shortened in group Ⅱ (P ＜ 0.05).Compared with groups Ⅰ and Ⅲ,TMT completion time was significantly shortened at 1 d after surgery in group Ⅱ (P ＜0.05).Conclusion The depth of sedation,40 ＜ BIS value ≤ 50,during combined intravenous-inhalational anesthesia with sevoflurane and remifentanil has less influence on postoperative cognitive function in patients undergoing gynecological laparoscopic surgery.

72 sera of highly sensitized antibody patients were detected by 35 samples of undonor lymphocytes before and after the dithothreitol (DTT) treating.The results showed that 72 sera reacted completely to B lymphocytes in both 4℃ and 37℃,but incompletely to T lymphocytes.After removal of the transplant kidney due to rejection,the sera antibody levels of T or B lymphocytes were higher than those of the patients'sera after blood transfusion or pregnancy.The serum T or B antibody levels of pregnancy were the lowest.In DTT-treated sera of highly sensitized patients,the antibodies were mixed types of IgG and IgM.

0.05).Furthermore,logistic regressive equations of prediction probability and RE,SE,BIS were calculated as ln [Pk/(1-Pk)]=-19.825+0.243x,ln [Pk/(1-Pk)]=-18.012+0.236x and ln [Pk/(1-Pk)]=-21.780+0.316x,respectively.From the perspective of sensitivity/specificity,RE,SE and BIS had threshold values for regaining consciousness of 83,76 and 71 with sensitivity/specificity of 0.90/0.85,0.89/0.80 and 0.88/0.78,respectively.RE was 30s earlier than BIS in predicting regaining consciousness.Conclusion Compared with BIS,entropy index seems to be a more accurate and sensitive electroencephalographic measure,and is promising in clinical application.

0.05),while the entropy index showed significant differences between the two groups(P